酵母硒对种公牛精液品质影响的研究

试验选用12头荷斯坦成年种公牛为试验牛,并随机分为四组。试验前期饲喂基础日粮,试验期添加不同剂量的酵母硒;90d时,采集种公牛的精液,并对精液量及精液品质进行检测。试验结果表明:日粮中适量添加硒能显著提高种公牛的精子冻前活率和冻后活率,并降低精子畸形率(P0.05),而对精液量、精子密度和精子顶体完整率无显著影响。     

浅论饲料企业质量管理体系长效机制的构筑

1饲料企业构筑质量管理体系长效机制的必要性1.1进一步全面宣贯ISO9000族标准的需要ISO9000族标准是系列标准,其结构互为补充。企业通过了质量管理体系的认证,仅仅证实企业具有提供"满足顾客要求"和"适用法律法规要求"产品的基本能力。从管理角度来看,这种质量管理体系尚不够完整,从提升企业总体业绩与     

锌源对凡纳滨对虾生长免疫的影响

凡纳滨对虾配合饲料中添加20、40、60、80和100mg/kg硫酸锌和蛋氨酸锌。饲养4和10周称质量,并采集对虾血清、肝胰脏和肌肉,测定血清中酚氧化酶(PO)和碱性磷酸酶(AKP)活性及肝胰脏和肌肉中的锌含量。试验结果表明,锌源和锌水平影响对虾4周末体质量,但对10周末的体质量无影响。锌源和锌水平不影响血清中的PO,但硫酸锌组AKP表现出随添加量的升高而显著提高,蛋氨酸锌组AKP差异不显著。2种锌源的添加水平对肌肉中的锌含量无影响,但对肝胰脏中锌含量影响显著。生长、酶活和组织锌含量的统计结果表明,锌源之间存在显著差异,蛋氨酸锌的营养效果好于硫酸锌。饲料中蛋氨酸锌的添加量为40~60mg/kg时,凡纳滨对虾的生长和免疫效果最好。     

Molecular taxonomic relationships of Psoroptes and Chorioptes mites from China based on COI and 18S rDNA gene sequences

In this present study, the mitochondrial DNA gene cytochrome coxidase subunit I (COI) and the small subunit ribosomal RNA (18S rDNA) gene were used to determine the taxonomic relationships of Psoroptes and Chorioptes mites from China. The neighbor-joining and maximum-parasimony approach were used to evaluate the evolutionary relatedness among different hosts in the genera Psoroptes and Chorioptes. Phylogenetic analysis showed that Psoroptes cuniculi and Psoroptes natalensis may be two different species within the genus Psoroptes, and Chorioptes texanus and Chorioptes panda are different species within the genus Chorioptes.     

生物纤维饲料在蛋鸡生产中的应用研究

生物纤维饲料是对玉米淀粉湿磨国工过程中的浸泡液，黄浆和玉米皮渣等副产品经生物发酵方法而形成的一种新型饲料。本文研究了生物纤维饲料在蛋鸡饲养中的饲喂效果。试验表明，半风干生物纤维饲料（含水分26．68％）以5％比例添加蛋鸡日粮中的饲喂效果与对照组相比，日均产蛋量提高6．4％，平均产蛋率提高6．05％，料蛋比下降13．01％，毛利润提高90．70％，而风干生物纤维饲料（含水分15．44％），则以10％比添加于蛋鸡日粮时，表现出较明显的增产效果，其与对照组相比，日均产蛋量提高4．04％，平均产蛋率提高4．18％，料蛋比下降4．09％，毛利润提高25．74％。     

动物福利法的历史起源、现状及意义

动物福利法的基本出发点是让动物在康乐的状态下生存,即减少其所受的不必要的痛苦,并且针对不同动物采取不同的福利标准,让人与动物和谐相处,从而推动社会的文明进步。本文对动物福利法的历史起源、现状及意义进行了叙述,并对动物福利法的立法宗旨、福利标准、结构框架及对中国法制进程的影响等诸方面进行了讨论。     

浅谈精细化管理在动物卫生检疫证照管理中的应用

检疫合格证明是动物产品溯源的重要途径,检疫证明的管理是否规范关系到动物卫生监督职能发挥的有效性。本文从检疫证照管理的具体实践中探讨引进精细化管理理念的优势,从而证明在精细化管理过程中,从"精细思维"的养成到"索证、验证、上缴核销"流程中的精细化操作,有效促进检疫证照管理的规范化、精细化,切实提高整个证照管理的科学性。     

小肽营养素对奶牛泌乳性能的影响

试验选择泌乳期中国荷斯坦奶牛32头,随机分为4组,A组(对照组)为基础精料日粮组;B、C、D组分别为基础精料日粮 0.1%、0.3%、0.5%小肽营养素。正试期28d。结果表明,添加0.1%、0.3%、0.5%小肽营养素后,奶牛头日平均产奶量分别提高1.67kg(6.38%)(P<0.05)、1.75kg(6.69%)(P<0.05)和1.64kg(6.27%)(P<0.05)。添加小肽营养素后,乳蛋白和乳脂率均有所提高,其趋势随小肽营养素添加量的增加而提高。添加0.1%、0.3%、0.5%小肽营养素后,奶牛头日净增效益3.52元、3.40元、2.86元。综合生产性能和经济效益,以添加0.1%-0.3%小肽营养素为适宜。     

Pharmacokinetics of mequindox and one of its major metabolites in chickens after intravenous, intramuscular and oral administration

Pharmacokinetics of mequindox and one of its major metabolites (M) was determined in chickens after intravenous (i.v.), intramuscular (i.m.) and oral administration of mequindox at a single dose of 10 (i.v. and i.m.) or 20 mg/kg b.w. (oral). Plasma concentration profiles were analyzed by a non-compartmental pharmacokinetic method. Following i.v., i.m. and oral administration, the areas under the plasma concentration-time curve (AUC(0-∞)) were 0.71±0.15, 0.67±0.21, 0.25±0.10 μg h/mL (mequindox) and 37.24±7.98, 36.40±9.16, 86.39±16.01 μg h/mL (M), respectively. The terminal elimination half-lives (t(1/2λz)) were determined to be 0.15±0.06, 0.21±0.09, 0.49±0.23 h (mequindox) and 5.36±0.86, 5.39±0.52, 5.22±0.35 h (M), respectively. The bioavailabilities (F) of mequindox were 89.4% and 16.6% for i.m. and oral administration. Steady-state distribution volume (V(ss)) of 1.20±0.34 L/kg and total body clearance (Cl(B)) of 13.57±2.16 L/kg h were determined for mequindox after i.v. dosing. After single i.m. and oral administration, peak plasma concentrations (C(max)) of 3.04±1.32, 0.36±0.13 μg/mL (mequindox) and 3.81±0.92, 5.99±1.16 μg/mL (M) were observed at t(max) of 0.08±0.02, 0.32±0.12 h (mequindox) and 0.66±0.19, 6.67±1.03 h (M), respectively. The results showed that mequindox was rapidly absorbed after i.m. or p.o. administration and most of mequindox was transformed to metabolites in chickens, with much higher C(max)s and AUCs of metabolite (M) than those of mequindox in plasma.     

Molecular cloning,chromosomal localization and expression pattern of porcine ADP‐ribosylation factor(Arf) gene family

Adenosine diphosphate‐ribosylation factors (Arfs) are a family of guanosine triphosphate‐binding proteins involved in fundamental biological processes including secretion, endocytosis, phagocytosis, cytokinesis, cell adhesion and tumor cell invasion. We report here the molecular cloning, chromosome localization and expression analysis of porcine Arf1–6, of which Arf1–3 (Class I) have >93% similarity to each other and encode nearly similar proteins with 181 amino acids in length. Arf4 and Arf5 (Class II) are 78–81% homologous to Class I Arfs and both encode a protein of 180 amino acids, Arf6 (Class III) shows 64–68% homology to the other Arfs and encodes 175 amino acids. With radiation hybrid mapping, porcine Arf1–6 are assigned to chromosomes 14q21‐q22, 12p14, 5p12‐q11, 13q21.1, 18q24, 1q21‐q27, respectively. Moreover, real‐time quantitative RT‐PCR assays show that porcine Arf1‐6 are ubiquitous in all tissues examined, with the highest levels in the kidney and stomach and the lowest in muscle and the heart. This is the first report of molecular characterization of the Arf gene family in pigs.