Typical and atypical manifestations of Anaplasma phagocytophilum infection in dogs

Eighteen clinically ill dogs, naturally infected with Anaplasma phagocytophilum, were examined at a veterinary practice in Baxter, Minnesota. A clinical examination, complete blood cell count, enzyme- linked immunosorbent assay (ELISA) for A phagocytophilum, Borrelia burgdorferi, and Ehrlichia canis antibodies and Dirofilaria immitis antigen, and a polymerase chain reaction test for A phagocytophilum DNA were obtained for all dogs. Physical examination findings included fever, arthropathy, lymphadenopathy, epistaxis, acute gastritis, cervical hyperpathia, and central nervous system dysfunction. Complete blood cell count abnormalities included thrombocytopenia, morulae in neutrophils, anemia, leukopenia, eosinopenia, lymphopenia, and monocytosis. Seroreactivity to A phagocytophilum was found in 61%, B burgdorferi antibodies in 17%, and D immitis antigen in 5% of the dogs. Fever, arthropathy, neurologic dysfunction, and epistaxis are clinical syndromes that can be associated with A phagocytophilum infection. Treatment with doxycycline resulted in rapid resolution of clinical signs in all dogs.     

Accumulation of specific flavonoids in soybean (Glycine max (L.) Merr.) as a function of the early tripartite symbiosis with arbuscular mycorrhizal fungi and Bradyrhizobium japonicum (Kirchner) Jordan

This study is the first report assessing the effect of soil inoculation on the signalling interaction of Bradyrhizobium japonicum, arbuscular mycorrhizal fungi (AMF) and soybean plants throughout the early stages of colonisation that lead to the tripartite symbiosis. In a study using soil disturbance to produce contrasting indigenous AMF treatments, the flavonoids daidzein, genistein and coumestrol were identified as possible signals for regulating the establishment of the tripartite symbiosis. However, it was unclear whether soil disturbance induced changes in flavonoid root accumulation other than through changing the potential for AMF colonization. In this study, soil treatments comprising all possible combinations of AMF and B. japonicum were established to test whether (1) modifications in root flavonoid accumulation depend on the potential for AMF colonization, and (2) synthesis and accumulation of flavonoids in the roots change over time as a function of the early plant-microbial interactions that lead to the tripartite symbiosis. The study was comprised of two phases. First, maize was grown over 3-week periods to promote the development of the AM fungus Glomus clarum. Second, the interaction between soybean, G. clarum and B. japonicum was evaluated at 6, 10, 14 and 40 days after plant emergence. Root colonization by G. clarum had a positive effect on nodulation 14 days after emergence, producing, 30% more nodules which were 40% heavier than those on roots solely inoculated with B. japonicum. The tripartite symbiosis resulted in 23% more N₂ being fixed than did the simpler symbiosis between soybean and B. japonicum. The presence of both symbionts changed accumulation of flavonoids in roots. Daidzein and coumestrol increased with plant growth. However, development of the tripartite symbiosis caused a decrease in coumestrol; accumulation of daidzein, the most abundant flavonoid, was reduced in the presence of AMF.     

Effects of different variable sets on the potential distribution of fish species in the Amazon Basin

Estimating species’ potential distribution is one of the main objectives of macroecology, especially when sampling biases can affect knowledge on how environmental variables affect species distribution. Ecological niche models estimate species’ environmental niches from different variables and their occurrences. Using the presence-only data from eight Amazonian fish species, which inhabit rivers and streams, we aimed to (a) explore the effect of different sets variables on the spatial distributions of target species and (b) evaluate the predictive responses of MaxEnt to sets of variables with different degrees of complexity. MaxEnt has high flexibility in relation to the input data and its performance is influenced by a moderate number of adjustable parameters, allowing for high precision results when balancing underestimation and overestimation errors. We used environmental predictors in MaxEnt the principal components of climatic, topographic and edaphic variables as inputs. The combination of topographic and edaphic variables produced more precise and spatially restricted distribution ranges for all species when compared to those generated with climatic variables. All models reached high AUC values, especially for stream species. Modelled range sizes were broader for the river species, suggesting different tolerance thresholds and habitat preferences when compared to stream species. The complexity of the different variables sets did not affect MaxEnt's prediction capacity. However, for stream species, MaxEnt showed a greater predictive power. This work increases the knowledge with regards to the influence of different environmental predictors on the spatial patterns of the distribution of Amazonian fish.     

Amplified fragment length polymorphism as a tool for molecular characterization of almond germplasm: genetic diversity among cultivated genotypes and related wild species of almond,and its relationships with agronomic traits

Amplified fragment length polymorphism (AFLP) analysis is a rapid and efficient method for producing DNA fingerprints and molecular characterization. Our objectives were to: estimate genetic similarities (GS), marker indices, and polymorphic information contents (PICs) for AFLP markers in almond cultivars; assess the genetic diversity of almond cultivars and wild species, using GS estimated from AFLP fingerprints and molecular characterization; and facilitate the use of markers in inter-specific introgression and cultivar improvement. The genetic diversity of 45 almond cultivars from Iran, Europe, and America, were studied assaying 19 primer combinations. In addition, several agronomic traits were evaluated, including flowering and maturity times, self-incompatibility, and kernel and fruit properties. Out of the 813 polymerase chain reaction fragments that were scored, 781 (96.23%) were polymorphic. GS ranged from 0.5 to 0.96, marker indices ranged from 51.37 to 78.79, and PICs ranged from 0.56 to 0.86. Results allowed the unique molecular identification of all assayed genotypes. However, the correlation between genetic similarity clustering as based on AFLP and clustering for agronomic traits was low. Cluster analysis based on AFLP data clearly differentiated the genotypes and wild species according to their origin and pedigree, whereas, cluster analysis based on agronomic data differentiated according the pomological characterization. Our results showed the great genetic diversity of the almond cultivars and their interest for almond breeding.     

Responses to water withdrawal of tobacco plants genetically engineered with the AtTPS1 gene: a special reference to photosynthetic parameters

We have previously obtained several lines of tobacco transformed with a trehalose-6-phosphate synthase gene of plant origin (Arabidopsis thaliana), involved in the first step of the biosynthesis of trehalose, a known osmoprotectant. Two showed distinct intensity of expression: high (B5H) and low (B1F). Such lines were analyzed for trehalose-6-phosphate content and the obtained results demonstrated to be in accordance with the expression results. In order to study the responses of photosynthesis to water deficit of transgenic lines in comparison to wild type (WT), three experiments were performed under different conditions: (1) Relative water (2) Leaf gas exchange (3) Modulated Chlorophyll a Fluorescence. Different responses in RWC of plant lines to water withdrawal were detected, with transgenic line B5H indicating less water loss after the water withdrawal period. Similar responses to water deficit regarding the leaf gas exchanges were recorded for the three lines. When subjected to water deficit stress situations, higher F _v/F _m, Φ_PSII and qP were detected for the transgenic lines. Under a SWC of 20% where higher values for such parameters were detected with special relevance for the B5H line, indicating a possible higher ability to withstand severe drought stress and to resist to prolonged periods without water than the B1F and WT lines.     

Inheritance and relationships of important agronomic traits in almond

The inheritance of 16 important agronomic traits and its relationship were studied for four years in a population of 167 almond [P. dulcis (Mill.) D.A. Webb] seedlings obtained from a cross between the French selection ‘R1000’ (‘Tardy Nonpareil’ × ‘Tuono’) and the Spanish cultivar ‘Desmayo Largueta’. For some traits (blooming and leafing date) descendants segregated between the value of both progenitors, meanwhile for others the mean of the offspring was lower (bloom density, productivity and ripening date) or higher (in-shell/kernel ratio and double kernels). As expected, kernel bitterness and self-incompatibility behaved as monogenic traits. Some important correlations between traits were detected. The implications of the transmission and the correlation of these traits in the breeding programmes are discussed.     

A male-fertile Lycopersicon esculentum × Solanum lycopersicoides hybrid enables direct backcrossing to tomato at the diploid level

Solanum lycopersicoides Dun. is a wild nightshade native to Chile and Peru that possesses many traits of potential interest to tomato breeders, including environmental stress tolerance, resistances to disease and insect pests, and certain fruit quality characteristics. Sexual and somatic hybrids with the cultivated tomato (Lycopersicon esculentum Mill.) are readily obtained, yet have a strong tendency towards male-sterility combined with unilateral incompatibility, barriers that have deterred breeding efforts in the past. We report herein the synthesis of a partially male-fertile F₁ hybrid by sexual crosses between tomato and a recently collected population of S. lycopersicoides. Over 280 BC₁ plants were obtained by directed backcrossing to tomato at the diploid level, of which 58 were sufficiently fertile to permit selfing or additional backcrosses. The transmission of S. lycopersicoides genetic markers into a uniform L. esculentum background was confirmed with 24 isozyme or morphological loci on 11 of the 12 alien chromosomes. Potentially useful tolerance to gray mold (Botrytis cinerea) was demonstrated by inoculation of stem cuttings with mycelial plugs: at 6 days post-inoculation, the intergeneric hybrid showed little evidence of disease progression and the length of stem lesions averaged only one third that of susceptible controls. This revised version was published online in July 2006 with corrections to the Cover Date.     

Structuring of some basic pedological processes

In the first section, the author presents a detailed analysis of fundamental processes of pedological evolution in the light of the latest findings as to biogeochemical, mineralogical and granulometric aspects of pedogenesis on crystalline aluminosilicate rocks. This analysis is based on the nature of various geochemical weathering phenomena, on the one hand, and on problems related to the organization of plasmic elements (pédoplasmation) and their possible transfers as particles, on the other. With reference to the latter aspect, two major types of “pedogenesis” may be considered: “associative” pedogenesis in which plasma-skeleton links are kept, and “dissociative” pedogenesis in which plasma—skeleton links are disrupted and plasma is redistributed.Then, in the second section, a structuring of fundamental pedogenetic processes is proposed, based on the observation of three major phyla at the earth surface.Phylum I: Process with predominant biogeochemical evolution without plasma formation or with plasma destruction.Phylum II: Process with predominant biogeochemical evolution with plasma formation and reorganization in situ.Phylum III: Process with predominant textural evolution with plasma mobilization and redistribution.     

A comparison between the spatial distribution pattern of flagellates and some physicochemical parameters in a waste stabilization pond

Protozoological and physicochemical analyses were performed in a waste stabilization pond at Sto. Tomas Atzingo, a small town near Mexico City, in order to discern the internal dynamics of the pond water through the study of the spatial distribution pattern of flagellates and the parallel water volume distribution pattern of some selected physicochemical determined parameters. The statistical method applied to the data obtained was by cluster analysis. The results showed a slight trend to heterogeneity in the system, when it was evaluated through the physicochemically determined parameters. On the other hand, the spatial distribution pattern of the flagellates pointed out a remarkable heterogeneity in the pond. In systems like the one studied where the main feature is an organic matter overload, such a difference in findings indicates a low correlation between the biological parameters, in this case the flagellates, and the physicochemical variables, that should be considered when evaluating the water quality of such systems, either through the physicochemical, the biological or both scopes.