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101.
102.
Lucia Prellwitz Fabiana Nunes Zambrini Jos Domingos Guimares Marco Antonio Paula de Sousa Maria Emília Franco Oliveira Alexandre Rosetto Garcia Srgio Novita Esteves Pawel Mieczyslaw Bartlewski Joanna Maria Gonalves Souza‐Fabjan Jeferson Ferreira Fonseca 《Reproduction in domestic animals》2019,54(9):1230-1235
This study compared the effects of intravaginal and intravenous routes of oxytocin (OT) administration in 46 oestrous‐induced Santa Inês ewes (6‐day treatment with progestin‐releasing intravaginal sponges and a single injection of 200 IU of eCG at the time of sponge removal) that underwent transcervical embryo recovery 6–7 days after oestrous onset and mating. All ewes received 37.5 μg of d‐cloprostenol via latero‐vulvar route, and 1 mg of oestradiol benzoate i.m. 16 hr before and 50 IU of OT 20 min before non‐surgical embryo recovery (NSER), with OT being administered intravenously (n = 21) or intravaginally (n = 21). An overall oestrous response was 95.6% (44/46), and adequate cervical retraction could be accomplished in 78.6% (33/42) of ewes. The percentage of successful NSER procedures was 57% (24/42) or 72.7% (24/33) of animals with sufficient cervical retraction. The duration of NSER procedure averaged 28 min (range: 17–40 min) and ~96% of flushing fluid could be recovered (range: 85%–100%). Out of 18 ewes that could not undergo NSER, 12 (66.6%) presented various anatomical barriers, whilst the other 33.4% did not present these barriers and still could not be traversed. Excluding the ewes with those anatomical features, the overall success rate of NSER was 80% (24/30). The route of OT administration had no effect on NSER efficiency or the ease with which transcervical embryo flushing was performed. Both routes of OT administration can be used for cervical dilation protocol. Discarding ewes with anatomical features precluding cervical penetration is highly recommended to increase the efficacy of NSER in sheep. 相似文献
103.
Corrêa AP Dossi AC de Oliveira Vasconcelos R Munari DP de Lima VM 《Veterinary parasitology》2007,143(3-4):267-274
The aims of this study were to evaluate the immunomodulatory role of TGF-β1, IL-10, and INF-γ in spleen and liver extracts and supernatant cultures of white spleen cells from male symptomatic and asymptomatic dogs, naturally infected by Leishmania (Leishmania) chagasi. Thirty dogs from Araçatuba, São Paulo, Brazil, an endemic leishmaniosis area, were selected by positive ELISA serological reaction for Leishmania sp. and divided into two groups: asymptomatic (n = 15) and symptomatic (n = 15) consisting of animals with at least three characteristic signs (fever, dermatitis, lymphoadenopathy, onychogryphosis, weight loss, cachexia, locomotion problems, conjunctivitis, epistaxis, hepatosplenomegaly, edema, and apathy). After euthanasia, spleen and liver fragments were collected for ex vivo quantification of TGF-β1, IL-10, and INF-γ. Naturally active in vitro produced TGF-β1 was also evaluated in spleen cell culture supernatant. Spleen and liver extract of asymptomatic dogs had higher mean TGF-β1 levels than symptomatic dogs. High concentrations of IL-10 were found in spleen, and mainly in liver extract of both groups. Higher INF-γ concentrations were found in spleen extracts of symptomatic dogs, and in liver extracts of asymptomatic dogs. Extract of this cytokine was lower in spleen extract. Although INF-γ is being produced in canine infection, mean levels of TGF-β1 and IL-10 from spleen and liver extracts were quantitatively much higher; suggesting that immune response in both asymptomatic and symptomatic dogs was predominantly type Th2. 相似文献
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Luis Artur Vales Bezerra Matheus Oliveira Freitas Vanessa Salete Daga Thiago Vinicius Trento Occhi Larissa Faria Ana Paula Lula Costa Andre Andrian Padial Viviane Prodocimo Jean Ricardo Simes Vitule 《Fish and Fisheries》2019,20(4):620-639
While environmental alterations have made Homo sapiens the hyperkeystone species of the globe, biotic homogenization initiated a new era, the “Homogenocene.” Still, some terrestrial and aquatic ecosystems in South America are considered pristine and wild, which can lead to a general faith that economic progress is consistent with conservation strategies, even without scientific support. We compiled anthropogenic threats to fish biodiversity in a hierarchical meta‐analysis, along with an evidence synthesis of threats related to biological invasions, based on peer‐reviewed research with the aim to represent the actual conservation status of the South American ichthyofauna. We highlighted human‐related threats and synergistic effects of biological invasions, climate change, environmental alterations (e.g., pollution, aquaculture and damming) and fisheries. Considering measures that reinforce novel alien fish (e.g., artificial hybrids or genetically modified) introductions, it became clear why an eventual increase in local or regional species richness is not always beneficial to aquaculture, biodiversity, human well‐being or nature. In fact, citizens in all societal roles, including scientists, should revise their concepts about threats to fish biodiversity. Environmental policies require more than taxonomic diagnostics to achieve conservation goals under an incompatible scenario of a multiplying number of fish species and biotic homogenization. We advocate for countries in South America using science‐based strategies useful to maintain their social and economic growth along with their “remaining nature.” We live a crucial moment when the government overlooks threats to biodiversity and uses agribusiness as the most acceptable manner of fuelling the economy. 相似文献
107.
Graciliana Lopes Georgios Daletos Peter Proksch Paula B. Andrade Patrícia Valent?o 《Marine drugs》2014,12(3):1406-1418
A monoacylglycerol (1) and a 1:1 mixture of two monogalactosyl diacylglycerols (MGDGs) (2 and 3) were isolated from the brown seaweed Fucus spiralis Linnaeus. The structures were elucidated by spectroscopic means (NMR and MS) and by comparison with the literature. Compound 1 was composed of a glycerol moiety linked to oleic acid (C18:1 Ω9). Compounds 2 and 3 contained a glycerol moiety linked to a galactose unit and eicosapentaenoic acid (C20:5 Ω3) combined with octadecatetraenoic acid (C18:4 Ω3) or linolenic acid (C18:3 Ω3), respectively. The isolated compounds were tested for their cytotoxic and anti-inflammatory activity in RAW 264.7 macrophage cells. All of them inhibited NO production at non-cytotoxic concentrations. The fraction consisting of compounds 2 and 3, in a ratio of 1:1, was slightly more effective than compound 1 (IC50 of 60.06 and 65.70 µg/mL, respectively). To our knowledge, this is the first report of these compounds from F. spiralis and on their anti-inflammatory capacity. 相似文献
108.
109.
Silva BM Andrade PB Ferreres F Domingues AL Seabra RM Ferreira MA 《Journal of agricultural and food chemistry》2002,50(16):4615-4618
Qualitative and quantitative analyses of phenolic compounds were carried out on quince fruit samples from seven different geographical origins in Portugal. For each origin, both pulp and peel were analyzed by reversed-phase HPLC-DAD and HPLC-DAD/MS.The results revealed differences between the phenolic profiles of pulps and peels in all studied cases. The pulps contained mainly caffeoylquinic acids (3-, 4-, and 5-O-caffeoylquinic acids and 3,5-dicaffeoylquinic acid) and one quercetin glycoside, rutin (in low amount). The peels presented the same caffeoylquinic acids and several flavonol glycosides: quercetin 3-galactoside, kaempferol 3-glucoside, kaempferol 3-rutinoside, and several unidentified compounds (probably kaempferol glycoside and quercetin and kaempferol glycosides acylated with p-coumaric acid). The highest content of phenolics was found in peels. 相似文献
110.