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71.
72.
Genetic variability in meiotic gynogenetic muskellunge,Esox masquinongy (Mitchell), estimated from segregation of microsatellite alleles
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Benjamin J Reading Paul S Wills Roy C Heidinger Edward J Heist 《Aquaculture Research》2016,47(9):2705-2715
Muskellunge, Esox masquinongy, is an important recreational freshwater fish native to North America. Since muskellunge populations are often maintained through stocking efforts, advances in muskellunge reproductive technologies are of direct relevance to fishery enhancement. We evaluated the efficiency of inbreeding through induced meiotic diploid gynogenesis. Eggs from six female muskellunge were manually stripped and activated using ultra violet‐irradiated yellow perch, Perca flavescens, sperm. Hydrostatic pressure shocking regimes (48 263 kPa) were then applied to the eggs to prevent second polar body expulsion producing unambiguous meiotic gynogens. Six female dams and samples of 12–20 of their gynogenetic progeny were genotyped at seven polymorphic microsatellite loci. Chromosomal recombination frequencies of microsatellite loci based on retention of heterozygosity among gynogens ranged from 0.043 to 0.839 (0.576 ± 0.237). There were no statistical differences in recombination frequency among females at any of the loci. The average inbreeding coefficient (F‐value) ranged from 0.581 to 0.979, equivalent to three to fourteen generations of full‐sibling crosses respectively. The average F‐value overall was 0.712, equivalent to between five and six generations of full‐sibling crosses. Centromere map distances of the seven microsatellite loci ranged from 2.15 to 41.95 cM and meiotic gynogenesis was useful in eliminating heterozygosity at loci proximal to the centromere, but not distal. Since the age at maturity of female muskellunge is approximately 5 years, gynogenesis may pose an expeditious alternative to traditional breeding strategies for creation of homozygous pedigrees for some loci that may be outcrossed to introduce positive heterozygosity effects in the offspring. 相似文献
73.
74.
Paul C. Stoy Mathew Williams Mathias Disney Ana Prieto-Blanco Brian Huntley Robert Baxter Philip Lewis 《Landscape Ecology》2009,24(7):971-986
Transferring ecological information across scale often involves spatial aggregation, which alters information content and
may bias estimates if the scaling process is nonlinear. Here, a potential solution, the preservation of the information content
of fine-scale measurements, is highlighted using modeled net ecosystem exchange (NEE) of an Arctic tundra landscape as an
example. The variance of aggregated normalized difference vegetation index (NDVI), measured from an airborne platform, decreased
linearly with log(scale), resulting in a linear relationship between log(scale) and the scale-wise modeled NEE estimate. Preserving
three units of information, the mean, variance and skewness of fine-scale NDVI observations, resulted in upscaled NEE estimates
that deviated less than 4% from the fine-scale estimate. Preserving only the mean and variance resulted in nearly 23% NEE
bias, and preserving only the mean resulted in larger error and a change in sign from CO2 sink to source. Compressing NDVI maps by 70–75% using wavelet thresholding with the Haar and Coiflet basis functions resulted
in 13% NEE bias across the study domain. Applying unique scale-dependent transfer functions between NDVI and leaf area index
(LAI) decreased, but did not remove, bias in modeled flux in a smaller expanse using handheld NDVI observations. Quantifying
the parameters of statistical distributions to preserve ecological information reduces bias when upscaling and makes possible
spatial data assimilation to further reduce errors in estimates of ecological processes across scale. 相似文献
75.
The unnaturally dark pigmentation of cultured Australian snapper Pagrus auratus can be improved through dietary astaxanthin supplementation and by holding fish in tanks with a white background. The practical application of these laboratory‐based findings was examined with two experiments to establish if the advantages of transferring fish to light coloured tanks before harvest could be achieved on‐farm using white cages and to determine the effects of fish density on skin colour. For the first experiment, snapper (mean TL=29.7 cm) were transferred from a commercial snapper sea cage to black or white netted cages and fed diets supplemented with unesterified astaxanthin (supplied as Lucantin® Pink, BASF) at 0 or 39 mg kg?1 for 42 days. Skin colour was measured using the CIE (black–white), (green–red), (blue–yellow) colour scale. Snapper held in white netting cages became significantly lighter (higher ) than snapper held in black cages; however, values were not as high as previous laboratory‐based studies in which snapper were held in white plastic‐lined cages. Snapper fed astaxanthin displayed significantly greater and values, and total carotenoid concentrations after 42 days. In addition, total carotenoids were higher in fish from black than white cages. The second experiment was designed to investigate whether density reduced the improvements in skin colour achieved by holding fish in white coloured cages and whether cage colour affected stress. Snapper (mean weight=435 g) were acclimated to black cages and fed 39 mg kg?1 astaxanthin for 44 days before transferring to black or white plastic‐lined cages at 14 (low), 29 (mid) or 45 (high) kg m?3 for 7 days after which time skin colour, plasma cortisol and plasma glucose concentrations were measured. Skin lightness () was greater in snapper transferred to white plastic‐lined cages with the lightest coloured fish obtained from the lowest density after 7 days. Density had no effect on plasma cortisol or glucose levels after 7 days, although plasma cortisol was elevated in snapper from black cages. For improved skin colouration we recommend feeding unesterified astaxanthin at 39 mg kg?1 for approximately 6 weeks and transferring snapper to white plastic‐lined cages or similar at low densities for short periods before harvest rather than producing fish in white netting sea cages subject to biofouling. 相似文献
76.
Toby G Knowles Steve N Brown Paul D Warriss Jeff Lines Ambrose Tinarwo Marta Sendon 《Aquaculture Research》2008,39(16):1731-1738
Food quality aspects of farmed turbot (Psetta maxima) were compared following two methods of slaughter: the current commercial method, by immersion in an ice slurry, which is then dewatered after approximately 20 min, or by first humanely, electrically stunning the fish using a prototype commercial stunner, before immersion in an ice slurry, which is dewatered after 20 min. Quality was assessed for up to 10 days of storage on ice following slaughter. No differences were found between the slaughter methods in terms of an overall carcass quality: overall appearance, haemorrhage, damage, burst gall bladder, staining of the body cavity by leakage from the gut or damage to the spine. No detectable difference was found between the treatments using the industry standard freshness scoring system, the Quality Index Method. Both groups of fish were classified as ‘Fresh’ after 10 days of storage on ice. Using objective measurements of colour, no differences between fish from either treatment were found in fillet colour. Changes in flesh pH were similar in electrically stunned and traditionally killed fish with a mean pH (±SE) at 2 h post‐mortem of 6.80±0.027 declining to 6.44±0.032 at 24 h post‐mortem. Humane electrical stunning of turbot at slaughter neither detectably improved nor decreased product quality as measured between 1 and 10 days of storage on ice. 相似文献
77.
A single‐factor experiment was conducted to investigate the effects of dietary astaxanthin concentration on the skin colour of snapper. Snapper (mean weight=129 g) were held in white cages and fed one of seven dietary levels of unesterified astaxanthin (0, 13, 26, 39, 52, 65 or 78 mg astaxanthin kg?1) for 63 days. Treatments comprised four replicate cages, each containing five fish. The skin colour of all fish was quantified using the CIE L*, a*, b* colour scale after 21, 42 and 63 days. In addition, total carotenoid concentrations of the skin of two fish cage?1 were determined after 63 days. Supplementing diets with astaxanthin strongly affected redness (a*) and yellowness (b*) values of the skin at all sampling times. After 21 days, the a* values increased linearly as the dietary astaxanthin concentration was increased before a plateau was attained between 39 and 78 mg kg?1. The b* values similarly increased above basal levels in all astaxanthin diets. By 42 days, a* and b* values increased in magnitude while a plateau remained between 39 and 78 mg kg?1. After 63 days, there were no further increases in measured colour values, suggesting that maximum pigmentation was imparted in the skin of snapper fed diets >39 mg kg?1 after 42 days. Similarly, there were no differences in total carotenoid concentrations of the skin of snapper fed diets >39 mg kg?1 after 63 days. The plateaus that occurred in a* and b* values, while still increasing in magnitude between 21 and 42 days, indicate that the rate of astaxanthin deposition in snapper is limited and astaxanthin in diets containing >39 mg astaxanthin kg?1 is not efficiently utilized. Astaxanthin retention after 63 days was greatest from the 13 mg kg?1 diet; however, skin pigmentation was not adequate. An astaxanthin concentration of 39 mg kg?1 provided the second greatest retention in the skin while obtaining maximum pigmentation. To efficiently maximize skin pigmentation, snapper growers should commence feeding diets containing a minimum of 39 mg unesterified astaxanthin kg?1 at least 42 days before sale. 相似文献
78.
Renata M. Barroso Paul A. Wheeler Scott E. LaPatra Robert E. Drew Gary H. Thorgaard 《Aquaculture (Amsterdam, Netherlands)》2008,277(3-4):156-163
Infectious hematopoietic necrosis virus (IHNV) is a major constraint to rainbow trout culture. Yellowstone cutthroat trout (Oncorhynchus clarki bouvieri) have greater resistance to this virus than do rainbow trout (O. mykiss), but the genetic mechanism of this resistance is not understood. We conducted a genome scan using a backcross of cutthroat trout into a rainbow trout background to estimate the number and locations of quantitative trait loci (QTL) associated with IHNV resistance and growth in trout. IHNV resistance was considered in terms of both survival (binary trait) and days to death (quantitative trait). The genetic map was scanned using interval mapping via two different approaches: one model considered survival alone and a second two-part model combined both survival and days to death. Three QTL were significantly (P ≤ 0.05) associated with virus resistance genome-wide, explaining 32.5% of the phenotypic variation. Cutthroat alleles at two of these QTL resulted in increased resistance to the pathogen, as expected. No growth QTL were detected in this cross. We suggest that these traits are genetically independent. 相似文献
79.
A review of cyanobacterial odorous and bioactive metabolites: Impacts and management alternatives in aquaculture 总被引:2,自引:0,他引:2
Juliette L. Smith Greg L. Boyer Paul V. Zimba 《Aquaculture (Amsterdam, Netherlands)》2008,280(1-4):5-20
Increased demand has pushed extensive aquaculture towards intensively operated production systems, commonly resulting in eutrophic conditions and cyanobacterial blooms. This review summarizes those cyanobacterial secondary metabolites that can cause undesirable tastes and odors (odorous metabolites) or are biochemically active (bioactive metabolites) in marine and freshwater, extensive and intensive aquaculture systems. For the scope of this paper, biochemically active metabolites include (1) toxins that can cause mortality in aquaculture organisms or have the potential to harm consumers via accumulation in the product (hepatotoxins, cytotoxins, neurotoxins, dermatoxins, and brine shrimp/molluskal toxins), (2) metabolites that may degrade the nutritional status of aquaculture species (inhibitors of proteases and grazer deterrents) or (3) metabolites that have the potential to negatively affect the general health of aquaculture species or aquaculture laborers (dermatoxins, irritant toxins, hepatotoxins, cytotoxins). Suggestions are made as to future management practices in intensive and extensive aquaculture and the potential exposure pathways to aquaculture species and human consumers are identified. 相似文献
80.
Sascha M.M. Fssler Natalia Gorska Egil Ona Paul G. Fernandes 《Fisheries Research》2008,92(2-3):314-321
Fish swimbladders, where present, contribute most to the scattering of sound by fish, as measured by the target strength (TS). The volumes of the swimbladders of two different European stocks of Atlantic herring were compared to consider the effect on estimates of TS. Swimbladder volumes of Baltic and Norwegian spring-spawning herring (Clupea harengus) were measured, together with individual herring fat content. Swimbladder volumes were found to differ significantly between the two herring stocks. Baltic herring have a larger swimbladder volume, which is suggested to be associated with the fish's low fat content, which in turn may be linked to its specific energy budget and the low salinity of the Baltic Sea. A buoyancy model that considered the different salinity conditions and fat proportions was used to evaluate the observed differences in swimbladder volume. The swimbladder volume data were subsequently used to model the mean target strength as a function of depth and growth pattern. Backscattering of the swimbladder was modelled using the modal-series-based deformed-cylinder model (MSB-DCM), describing the swimbladder as a gas-filled, elongated prolate spheroid. The fish body component was modelled as a fluid-filled ellipsoid using the distorted wave Born approximation (DWBA). Modelling results support a different TS-to-size relationship for Baltic herring, with a stronger echo, due to the larger swimbladder. Depth- and length-dependent TS relationships based on the model results are suggested. 相似文献