Early tree growth, crop yields and estimated returns for an agroforestry trial in Goldsboro, North Carolina

A 17 acre (6.9?ha) agroforestry research and extension alley cropping trial was established at the Center for Environmental Farming Systems in Goldsboro, North Carolina in January 2007, with a randomized block design with five replications. Loblolly pine (Pinus taeda), longleaf pine (Pinus palustris) and cherrybark oak (Quercus pagoda) were planted in staggered rows, with each species planted for 140?ft (43?m) per replication. Crop land alleys of 40?ft or 80?ft (12.2–24.4?m) wide were left between the tree rows. Crops of soybeans (Glycine max) and corn (Zea mays) were planted in alternating years since establishment. As of 2011, survival rates were 93% for cherrybark oak, 88% for longleaf pine and 97% for loblolly pine. Average tree diameter at ground level was 1.0?in (2.5?cm) for cherrybark oak, 2.1?in (5.3?cm) for longleaf and 3.2?in (8.1?cm) for loblolly. Heights averaged 4.6?ft (1.4?m) for cherrybark oak, 5.2?ft (1.6?m) for longleaf pine and 10.4?ft (3.2?m) for loblolly pine. Growth, yield and economic projections for traditional timber production indicated that species volumes and values tracked the height and diameter relationships measured on the site. Loblolly pine had the largest projected internal rate of return, at 7.2%, followed by longleaf pine with pine straw harvests at 5.5%, longleaf without pine straw at 3.5% and cherrybark oak at 1.9%. There might be more loss in crop and silvopasture production with loblolly, however, and production of pine straw for longleaf or game mast for cherrybark oak may offer other benefits. Crop yields on the sandy soils on the site were very poor during the 4?years observed, which had a series of droughts and floods. These led to net financial losses in those years for the demonstration site, but state-wide average farm budget returns did show moderate profits. The results support the merits of agroforestry systems in the upper South to diversify income and reduce financial risks.     

First report on the autochthonous gut microbiota of brown trout (Salmo trutta Linnaeus)

A study was conducted to characterize the autochthonous gut microbiota present in the pyloric caeca (PC), anterior mucosa (AM) and posterior mucosa (PM) of brown trout (Salmo trutta). Total viable counts (TVC) bacterial populations were enumerated using tryptone soy agar, lactic acid bacteria (LAB) levels were enumerated on de Man, Rogosa & Sharpe agar and PCR‐DGGE was employed as a culture‐independent method to assess the total communities. No significant differences were observed between the different gut regions for TVC or LAB levels. 16S rRNA sequencing identified all LAB isolates as Carnobacterium maltaromaticum. In contrast, the TVC community was more diverse; Firmicutes and Bacteroidetes were present but all gut regions were dominated by Proteobacteria, accounting for 88.4–92.6% of the communities. Citrobacter freundii was the dominant species and accounted for 51.0–57.8% of the isolates. Complex bacterial communities were observed using PCR‐DGGE and a trend towards the reduction in the number of operational taxonomic units (OTUs), microbial richness and microbial diversity was observed from the PC to the PM. The similarity between regions was low (52–68%) and cluster analysis revealed that the communities grouped into two distinct clusters; one dominated by the PM samples and the other contained the AM and PC samples. OTUs from the DGGE were identified as members of the phyla Proteobacteria and Firmicutes. Many OTUs were detected in all gastrointestinal regions, however, some OTUs showed regional specialization. Further studies are required to elucidate the activity of these genera in situ and how their actions impact the host.     

A comparative review of fisheries management experiences in the European Union and in other countries worldwide: Iceland,Australia, and New Zealand

This study compares the details and performance of fisheries management between the EU and a selection of other countries worldwide: Iceland, New Zealand, and Australia, which are considered in many respects to be among the most advanced in the world in fisheries management. Fisheries management in the EU, Iceland, Australia, and New Zealand has developed following different paths, despite being based on similar instruments and principles. Iceland, Australia, and New Zealand have been at the forefront of developing management practices such as stakeholder involvement, legally binding management targets (Australia, New Zealand), individual transferable quotas, and discard bans (Iceland, New Zealand). The EU has since the beginning of the 21st century taken significant steps to better involve stakeholders and establish quantitative targets through management plans, and a landing obligation is gradually being implemented from 2015 onward. The management of domestic fisheries resources in Australia, New Zealand, and Iceland has, overall, performed better than in the EU, in terms of conservation and economic efficiency. It should, however, be stressed that, compared to Australia, New Zealand, and Iceland, (i) initial over‐capacity was more of an issue in the EU when management measures became legally binding and also that (ii) EU has been progressive in developing common enforcement standards, on stocks shared by sovereign nations. The situation of EU fisheries has substantially improved over the period 2004–2013 in the northeast Atlantic, with fishery status getting close to that in the other jurisdictions, but the lack of recovery for Mediterranean fish stocks remains a concern.     

Mate selection in aquaculture breeding using differential evolution algorithm

An algorithm to perform mate selection in aquaculture breeding using a computational optimization procedure called “differential evolution” (DE) was applied under optimum contribution selection and mate selection scenarios, to assess its efficiency in maximizing the genetic merit while controlling inbreeding. Real aquaculture data sets with 8,782 Nile tilapias from five generations and 79,144 coho salmon from eight generations were used to optimize objective functions accounting for coancestry of parents and expected genetic merit and inbreeding of the future progeny. The mate selection results were compared with those from the realized scenario (real mates), truncation selection and optimum contribution selection method. Mate selection allowed reducing inbreeding up to 73% for Nile tilapia, compared with truncation selection, and up to 20% for coho salmon, compared with realized scenario. There was evidence that mate selection outperformed optimum contribution selection followed by minimum inbreeding mating in controlling inbreeding under the same expected genetic gain. The developed algorithm was computationally efficient in maximizing the objective functions and flexible for practical application in aquaculture breeding.     

Flow‐mediated movement of freshwater catfish,Tandanus bostocki,in a regulated semi‐urban river,to inform environmental water releases

Movement and migration of fish are critical for sustaining riverine fish populations. Water resource development alters natural flow regimes and can disconnect habitats and interfere with hydrological cues for fish movement. Environmental flow releases can counter these impacts, but to be effective they must be based on quantitative flow–biota relationships. We used radio‐telemetry to investigate the association between flow and movement of Tandanus bostocki, a plotosid fish endemic to south‐western Australia. Movement was assessed for 15 adult fish at three temporal scales: weekly, daily and bihourly to reveal seasonal patterns in movement, movement around individual flow pulses, and to describe changes in home range respectively. We used a predictive modelling approach to assess the importance of discharge and other covariates on the directional distance travelled or linear home range size. Our seasonal and flow pulse study revealed that T. bostocki undertook larger downstream movements during higher flows and smaller upstream movements during lower flows. Daily movements tended to be downstream on the ascending limb of flow pulses and upstream on the descending limb. Flow‐dependent movements at weekly or daily time scales were relatively modest (typically hundreds of metres) and were moderated by time of year and gender; however, fish underwent a synchronised 1‐km movement upstream during the known reproductive period in October. The home range study revealed that T. bostocki had larger home ranges (night‐time foraging) when baseflow was elevated. These results can assist the design of customised environmental flows in the study river and other flow‐regulated rivers.     

Effects of Various Dietary Carotenoid Pigments on Fillet Appearance and Pigment Absorption in Channel Catfish, Ictalurus punctatus

A study was conducted to evaluate effects of various carotenoids on skin and fillet coloration and fillet carotenoid concentration in channel catfish, Ictalurus punctatus. For 12 wk, juvenile catfish were fed one of six experimental diets containing no supplemental carotenoid or 100 mg/kg of one of following carotenoid additions: β‐carotene (BCA), lutein (LUT), zeaxanthin (ZEA), canthaxanthin (CAN), and astaxanthin (AST). Visual yellow color intensity score was highest for fish fed LUT, followed by ZEA, AST, and CAN, and lowest for fish fed basal and BCA diets. Skin and tissue Commission Internationale de I’Eclairage yellowness value was the highest in fish fed LUT, followed by fish fed ZEA, AST, and CAN, and lowest for fish fed basal and BCA diets. Fish accumulated the supplemental carotenoids in muscle tissues, but concentrations of different carotenoids in the tissue varied greatly. Approximately 30% of the LUT added was converted to echineone; no conversion was observed among other supplemental carotenoids. Results from the present study indicate that channel catfish can accumulate yellow pigments LUT and ZEA and red or pink pigments CAN and AST in the flesh, resulting in yellow coloration. The yellow pigment BCA does not appear to deposit in skin or flesh at levels sufficient to alter the coloration.     

Effects of spatial heterogeneity and subsample pooling on the measurement of abiotic and biotic soil properties in rainforest,pasture and reforested sites

Spatial variability presents a challenge for effective measurement of ecological processes in soil ecosystems. This study characterized the heterogeneity of seven different biotic and abiotic soil properties at three ecological scales: land‐use type (vegetation type), site (scale of km to tens of km, and subplot (10–30 m). We addressed three questions about soil properties: (i) Do they differ more at any ecological scale when compared with other scales? (ii) Does physically combining samples from replicate subplots affect their measurement? (iii) What are the implications of this variation for the detection of differences caused by changing land‐use? There were five land‐use types: remnant rainforest, pasture, ecological restoration plantings 10–15 yrs old, 40–50 yrs regrowth dominated by the non‐native tree camphor laurel (Cinnamomum camphora) and rainforest regrowth 3–6 yrs after poisoning of camphor trees within older regrowth. Sites were interspersed within a 750 km² region of subtropical Eastern Australia. In general, variability was greatest at the site and subplot levels. Soil water content, soil organic matter and pH were less variable than total organic carbon, microbial biomass, nitrate and nitrification. Power analyses using total organic carbon and nitrate showed that adequate replication at both site and subplot levels was important for detecting effects of land‐use type, although increased replication was slightly more effective for subplots than for sites. Subplot spacing was relatively unimportant. Physically combining subplot samples to yield one aggregate measurement per site was successful in reducing analytical effort without sacrificing accuracy for all soil properties except total organic carbon and nitrification.     

Myeloma-related disorders in cats commonly present as extramedullary neoplasms in contrast to myeloma in human patients: 24 cases with clinical follow-up

BACKGROUND: Myeloma-related disorders (MRD) are rare neoplasms of plasma cells. Published case reports describe a diversity of clinical presentations with confusing terminology and diagnostic criteria as a consequence of the assumption that MRD in cats are analogous to those in dogs or humans. OBJECTIVE: The aim of the study was to describe clinical, clinicopathologic and imaging findings, response to treatment, survival and possible associations with other diseases or vaccination in a large case series. A priori hypotheses were that cats with MRD commonly present with extramedullary involvement and uncommonly have radiographic bone lesions, in contrast to human patients. ANIMALS: Twenty-four cats with MRD confirmed by cytology or histopathology and immunohistochemistry. METHOD: A multicenter retrospective study was performed. RESULTS: Two types of clinical presentation were observed. The first group (n = 17) had neoplasia involving abdominal organs, bone marrow, or both. All developed systemic clinical signs and paraproteinemia. Five of 7 cats that received chemotherapy improved clinically or had decreased serum globulin concentration (median survival, 12.3 months; range, 8.5-22 months). The second group comprised 7 cats with skin masses, 2 of which were paraproteinemic and developed rapidly worsening systemic signs. In cats without systemic signs, excision of the skin masses appeared to be associated with prolonged survival (up to 2.4 years). Cats with MRD commonly presented with extramedullary involvement (67%), versus humans with MRD (5%) (P < .001), and uncommonly presented with radiographic bone lesions (8%) versus humans with MRD (80%) (P < .001). CONCLUSIONS: Radiographic bone lesions are uncommon in cats with MRD and extramedullary presentation is common, relative to human myeloma.