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101.
Anna Carbone Barbara Parrino Gloria Di Vita Alessandro Attanzio Virginia Spanò Alessandra Montalbano Paola Barraja Luisa Tesoriere Maria Antonia Livrea Patrizia Diana Girolamo Cirrincione 《Marine drugs》2015,13(1):460-492
Two new series of nortopsentin analogues, in which the imidazole ring of the natural product was replaced by thiazole and indole units were both substituted by 7-azaindole moieties or one indole unit was replaced by a 6-azaindole portion, were efficiently synthesized. Compounds belonging to both series inhibited the growth of HCT-116 colorectal cancer cells at low micromolar concentrations, whereas they did not affect the viability of normal-like intestinal cells. A compound of the former series induced apoptosis, evident as externalization of plasma membrane phosphatidylserine (PS), and changes of mitochondrial trans-membrane potential, while blocking the cell cycle in G2/M phase. In contrast, a derivative of the latter series elicited distinct responses in accordance with the dose. Thus, low concentrations (GI30) induced morphological changes characteristic of autophagic death with massive formation of cytoplasmic acid vacuoles without apparent loss of nuclear material, and with arrest of cell cycle at the G1 phase, whereas higher concentrations (GI70) induced apoptosis with arrest of cell cycle at the G1 phase. 相似文献
102.
Patrizia Digiovinazzo Gentile Francesco Ficetola Luciana Bottoni Carlo Andreis Emilio Padoa-Schioppa 《Forest Ecology and Management》2010
Fragmentation is a major cause of biodiversity loss. The relationship between fragmentation and biodiversity might be non-linear, due to the presence of ecological thresholds; however, the application of the threshold concept on the conservation of herb biodiversity has received limited attention. We assessed the relationship between a pool of woodland indicators and features of forest fragments (area, isolation, shape), and we evaluated whether ecological thresholds might provide guidelines for conservation. We performed our study in a human dominated area of northern Italy. In order to obtain a group of floristic indicators, we chose perennial herbs characteristic of the phytosociological syntaxa Fagetalia sylvaticae, Carpinion betuli and Erythronio-Carpinion, with suitable Landolt L and H index, obtained from previous relevés in reference and high-quality forests. Subsequently, we analyzed the relationship between richness of indicators and patch features recorded in a highly fragmented landscape. Generalized linear models showed that the number of forest indicators was positively related to fragment size and connectivity. Generalized Additive Models showed that the relationship between indicator richness and fragment size was non-linear, with the presence of two thresholds. The number of forest indicators increased until 35–40 ha; above this size the richness remained constant. Fragments above this threshold should be the target of conservation. Furthermore, the relationship between area and number of indicators was less steep in patches smaller than 1–1.5 ha. Patches between these two thresholds can be optimal targets of management actions, as small size increases can greatly improve their value for biodiversity. The identification of two thresholds in the relationship between patch area and indicators suggests an abrupt response to changing landscape features and provide quantitative targets for conservation. 相似文献
103.
Use of biocontrol agents and botanicals in integrated management of Botrytis cinerea in table grape vineyards
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104.
Alessandra Turrini Cristiana Sbrana Patrizia Strani Beatrice Pezzarossa Rosalba Risaliti Manuela Giovannetti 《Biology and Fertility of Soils》2010,46(5):511-520
In this work we have determined the community composition of spore-forming arbuscular mycorrhizal fungi (AMF) in a maquis
site on Pianosa island, a protected area within the Tuscan Islands UNESCO Biosphere Reserve, Italy. We have analysed rhizosphere
soil of the dominant plant species Pistacia lentiscus, Smilax aspera, Rosmarinus officinalis and of the endemic plant Helichrysum litoreum. The AMF species recovered were: Scutellospora dipurpurescens, Glomus coronatum, Glomus mosseae, Glomus etunicatum, Glomus geosporum, Glomus viscosum, Entrophospora sp., Pacispora sp. and Glomus rubiforme. The identification of native S. dipurpurescens and G. coronatum was carried out on spores isolated from rhizosphere soil of H. litoreum, by combining morphological traits and 18S (SSU) and ITS rDNA sequences. Therefore, AMF species of Pianosa rhizosphere soils
represent an important repository for the conservation and maintenance in their natural habitat of such beneficial symbionts,
key microorganisms of soil fertility. 相似文献
105.
Patrizia Di Filippo Carmela Riccardi Donatella Pomata Claudio Gariazzo Francesca Buiarelli 《Water, air, and soil pollution》2010,211(1-4):231-250
Polycyclic aromatic hydrocarbons (PAHs); their derivatives nitro, and methyl-PAHs; n-alkanes; and organic acids were investigated in the aerosol samples collected during two field campaigns conducted at three sampling stations in an industrialized city in southern Italy. The main sources affecting the atmosphere and its toxicity were investigated by means of the diagnostic ratios of: specific particulate-phase PAHs, marker compounds among nitro-PAHs, alkanes, and acids, the dominant wind direction, daily and seasonal abundance of carcinogenic organic substances. The potential importance of the non-regulated pollutants to assess the air quality was confirmed; in fact the carcinogenic organic compounds showed to have scarce correlation with particulate matter (PM) concentration. An exceptionally high variability of toxic compounds at a daily scale was due to meteorological condition causing periods of extremely high pollution levels. 相似文献
106.
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108.
Fabiani R Sepporta MV Mazza T Rosignoli P Fuccelli R De Bartolomeo A Crescimanno M Taticchi A Esposto S Servili M Morozzi G 《Journal of agricultural and food chemistry》2011,59(15):8167-8174
One of the main olive oil phenolic compounds, hydroxytyrosol (3,4-DHPEA), exerts in vitro chemopreventive activities (antiproliferative and pro-apoptotic) on tumor cells through the accumulation of H(2)O(2) in the culture medium. However, the phenol composition of virgin olive oil is complex, and 3,4-DHPEA is present at low concentrations when compared to other secoiridoids. In this study, the in vitro chemopreventive activities of complex virgin olive oil phenolic extracts (VOO-PE, derived from the four Italian cultivars Nocellara del Belice, Coratina, Ogliarola, and Taggiasca) were compared to each other and related to the amount of the single phenolic constituents. A great chemopreventive potential among the different VOO-PE was found following this order: Ogliarola > Coratina > Nocellara > Taggiasca. The antiproliferative and pro-apoptotic activities of VOO-PE were positively correlated to the secoiridoid content and negatively correlated to the concentration of both phenyl alcohols and lignans. All extracts induced H(2)O(2) accumulation in the culture medium, but this phenomenon was not responsible for their pro-apoptotic activity. When tested in a complex mixture, the olive oil phenols exerted a more potent chemopreventive effect compared to the isolated compounds, and this effect could be due either to a synergistic action of components or to any other unidentified extract constituent. 相似文献
109.
Barbiroli A Bonomi F Ferranti P Fessas D Nasi A Rasmussen P Iametti S 《Journal of agricultural and food chemistry》2011,59(10):5729-5737
Fatty acids are the natural ligands associated with the bovine milk lipocalin, β-lactoglobulin (BLG), and were identified by means of mass spectrometry. The naturally bound ligands were found to contribute to the stability of the proteins toward denaturation by both temperature and chaotropes. To assess the nature of the structural regions involved in this stabilization, the thermodynamic and kinetic aspects of the stability of various structural regions of the proteins were studied in the presence of bound palmitate, which is the most abundant natural ligand. Binding of a single palmitate molecule was found to affect not only the stability of the calyx region, where palmitate is bound, but also that of the region at the hydrophobic interface between the barrel itself and the long helix in the protein structure, where the thiol group of Cys121 is buried. This region is known to be essential for the stability of the BLG dimer and is relevant to the generation of "reactive monomers" that are involved in covalent and noncovalent polymerization of BLG and in the formation of covalent adducts with other milk proteins. 相似文献
110.
Giovanni Tabacchi Lucio Di Cosmo Patrizia Gasparini 《European Journal of Forest Research》2011,130(6):911-934
In this article, we present equations derived for the prediction of the aboveground tree volume and phytomass for twenty-five
of the most important forest species growing in Italy. These equations result from ongoing research aiming to fill a gap in
the models available at the national scale. With regard to volume, the results are particularly important for thirteen species
or groups of species that were once scaled with models, conventionally assumed as reference models, available for other species.
In Italy, phytomass models had never been constructed at the national level before. For any single tree, specific equations
allow estimations of the following tree components to be made: stem and large branches (for either volume or phytomass), small
branches (phytomass), stump (phytomass) and the whole tree phytomass. The models have been constructed on the basis of nearly
1,300 sampling units (sample trees). Although these equations must be considered intermediate results of the ongoing research
because only half the scheduled number of samples has been collected, they have already been used in the practice, for example
in the estimates reported in the recently published second national forest inventory. 相似文献