Effects of Neem Seed Derivatives on Brown Planthopper Symbiotes

Populations of intracellular symbiotes declined significantly in brachypterous females of the brown planthopper,Nilaparvata lugens (Stål), which were stressed during nymphal development by caging them on IR20 rice plants treated with 3% neem oil, 5% neem seed kernel aqueous extract, or 10% neem cake aqueous extract. In addition, nymphs grew poorly and symbiote populations in prospective females declined, when reared on rice plants grown in soil treated with neem cake; this indicates systemic action of bioac-tive neem constituents. Addition of custard-apple oil to neem oil did not enhance the inhibitory effects. The effects of neem derivatives on the symbiotes may be either host-mediated — possibly through disturbed neuroendocrine homeostasis, or direct — leading to perturbations of host-symbiote equilibrium.     

Pathogenic mechanisms of caprine arthritis-encephalitis virus

Goats infected with caprine arthritis-encephalitis virus (CAEV) show chronic arthritis and cachexia, which are progressive in nature. The immunopathogenic mechanisms responsible for these progressive clinical symptoms have not been fully elucidated. Various haematological and immunological parameters were evaluated in experimentally-infected goats showing typical signs of CAEV-induced disease. Infected goats showed recurrent lymphocytosis that may be due to constant presentation of antigen by infected cells of a monocyte/macrophage lineage. The serum alkaline phosphatase and -glutamyl transferase concentrations were elevated in infected goats, a characteristic of hepatic and bone disorders. All other serum chemistry parameters were similar between infected and control goats. Importantly, the serum tumour necrosis factor- (TNF-) levels were higher in infected goats. The cachexia seen in infected goats may be at least partly due to altered metabolism as a result of prolonged elevation of serum TNF- levels. Depressed natural killer cell activity was observed in infected goats and may contribute towards the establishment of a persistent infection with CAEV.Abbreviations AIDS acquired immunodeficiency syndrome - CAEV caprine arthritis-encephalitis virus - GGT -glutamyl transferase - HBSS Hanks' balanced salt solution - HIV human immunodeficiency virus - NK natural killer - PBMC peripheral blood mononuclear cells - PCR polymerase chain reaction - SAP serum alkaline phosphatase - TNF tumour necrosis factor     

A formal method of determining the dietary amino acid requirements of laying‐type pullets during their growing period

1. The amino acid requirements of laying type pullets during the growing period can be estimated by measuring the growth of different components of the body and making use of nutritional constants that define the amount of each amino acid that is required for the production of the tissues being formed.

2. In this experiment, carcase analyses of each of three breeds of pullets were conducted at weekly intervals throughout the growth of the pullets, to 18 weeks of age. Measurements were made of body weight, gut‐fill and feather weight, and chemical analyses consisted of water, protein, lipid and ash measurements of both the body and the feathers. Each age group comprised 10 birds of each breed.

3. Gompertz functions accurately estimated the growth of both body protein and feather protein, to 18 weeks of age, from which the rate of growth of these two components of the body could be estimated. The mature weight of pullets was overestimated by the Gompertz growth curve, which may indicate that a pullet ceases to increase in body protein content once sexual maturity has been reached.

4. Using allometric relationships between the chemical components of the body and of feathers, all the components of growth could be estimated from the growth of body protein and feather protein. These components were then added together to determine the growth rate of the body as a whole.

5. The daily amino acid requirements for 4 functions were calculated, namely, those for the maintenance of body protein and feather protein, and for the gain in body protein and feather protein. These requirements were then summed to determine the requirement of pullets on each day of the growing period.

6. Using the ‘effective energy’ system, the amount of energy required by these pullets was calculated for each day of the growing period, from which the desired daily food intake of the pullets could be predicted. By dividing the amino acid requirement by this daily food intake it was possible to determine the concentration of amino acids that would be needed in the diet in order to meet the requirements of a pullet.

7. The results indicate that the ratio between the requirement for lysine and for methionine and cysteine changes dramatically during the growing period, negating the concept of a fixed ratio between all the amino acids during growth.

8. The above process is the first step in determining the optimal feeding programme for a population of pullets of a given genotype. The constraining effects, of the diet being offered and of the environment in which the pullets are housed, on the food intake and growth rate of each pullet have to be estimated, and such a theory can then be expanded to include all the individuals in the population. Only by the use: of simulation models can all these constraining effects be considered simultaneously.     

Chylous effusions revisited

Varying terms and criteria have been used in the veterinary literature to characterize milky opaque pleural effusions through the years. This article addresses ideas widely repeated in the veterinary and human literature upon which time, experience, diagnostic techniques, experimental data, and improved understanding of pathogenesis have cast doubt. Topics discussed include terminology, pathogenesis of chylous and pseudochylous effusions, criteria for differentiation of chylous from pseudochylous effusions, and clinicopathologic changes associated with drainage of chylous effusions.     

Structure of solidified colloidal array laser filters studied by cryogenic transmission electron microscopy

Ordered arrays of charged uniform colloidal polystyrene particles in solution form a single-wavelength laser filter that is transparent to other wavelengths. A usable filter was prepared by solidification within an acrylamide-methylene-bisacrylamide gel. The rejection wavelength of the colloidal array filter changes with gel composition. Cryogenic transmission electron microscopy of two gel formulations shows that the colloidal array filter is composed of AAA stacking of close-packed planes. Excellent agreement is found between the layer spacings measured and those predicted from the rejection wavelengths.