温度对新疆鬣蜥热能代谢及甲状腺素含量的影响

以新疆鬣蜥(Agamastoliczkana)为材料,当环境温度分别是低温(5±0.5℃)、室温(15±0.5℃)和高温(30±0.5℃)时,对基础代谢率和甲状腺素(T3,T4)含量进行测定。结果表明,新疆鬣蜥基础代谢率和甲状腺素含量与环境温度正相关,处在低温、室温、高温时,基础代谢率分别为15.82±0.77(S.E)mLO2·kg-1h-1,26.65±2.28(S.E)mLO2·kg-1h-1,69.89±3.43(S.E)mLO2·kg-1h-1。血浆甲状腺素(T3)分别为0.12±0.01(S.E)ng/mL,0.21±0.07(S.E)ng/mL,0.28±0.13(S.E)ng/mL;(T4)分别为3.63±0.58(S.E)ng/mL,8.73±1.1(S.E)ng/mL,21.81±1.82(S.E)ng/mL。同时发现新疆鬣蜥基础代谢率和甲状腺素含量与其活动程度一致。     

Composition and Antimicrobial Activity of Anemopsis californica leaf oil

Isolation and characterization of leaf volatiles in Anemopsis californica (Nutt.) Hook. and Arn. (A. californica) was performed using steam distillation, solid-phase microextraction, and supercritical fluid extraction. Thirty-eight compounds were detected and identified by gas chromatography; elemicin was the major component of the leaf volatiles. While the composition of the leaf volatiles varied with method of extraction, alpha-pinene, sabinene, beta-phellandrene, 1,8-cineole, piperitone, methyl eugenol, (E)-caryophyllene, and elemicin were usually present in readily detectable amounts. Greenhouse-reared clones of a wild population of A. californica had an identical leaf volatile composition with the parent plants. Steam-distilled oil had antimicrobial properties against 3 (Staphylococcus aureus, Streptococcus pneumoniae, and Geotrichim candidum) of 11 microbial species tested. Some of this bioactivity could be accounted for by the alpha-pinene in the oil.     

Alleviation of soil acidity improves the performance of oil palm progenies planted on an acid Ultisol

Abstract

Soil acidity is one of the main factors that limit profitable and sustained agricultural production. Oil palm (Elaeis guineensis Jacq.) is mainly planted in acidic soils. In the last years, there has been a stagnated yield and increases in disease incidence and severity worldwide that could be attributed in some extent to soil acidity. This study was conducted to determine the effects of soil acidity alleviation on oil palm seedlings. The effects of ground magnesium limestone or dolomite and magnesium carbonate (0, 1.1, 2.2, 3.3 and 4.4 t ha^?1) applied to an Ultisol dominated by kaolinite (pH in water 4.4) were evaluated on selected morphological, physiological and nutritional characteristics of hybrid (Deli dura × AVROS pisifera) and clonal (clone 366) oil palm progenies under nursery conditions for 8 months. Increasing rates of ground magnesium limestone and magnesium carbonate showed a significant effect on improving soil pH and lowering exchangeable aluminium. The hybrid oil palm showed significant either linear or quadratic trends for most of the parameters evaluated, indicating that the best responses for morphological and physiological traits were achieved from 2.5 to 4.23 t ha^?1 with ground magnesium limestone and 2.87 to 3.45 t ha^?1 with magnesium carbonate. Positive effects of increasing rates of ground magnesium limestone and magnesium carbonate were observed on nitrogen and magnesium uptake. Aluminium concentration in the third frond decreased significantly with increasing ground magnesium limestone rate. A significant reduction of manganese uptake was also observed with increasing rates of both ameliorants. The clonal oil palm progeny exhibited a better performance on un-amended treatment. This may be explained by the significant higher root growth of this progeny. Soil acidity alleviation improved the oil palm seedling growth. These results are important for the oil palm industry and could be applied in the nursery stage as well as extended to the immature stage.     

Comparison of the mucosal immune response in dogs vaccinated with either an intranasal avirulent live culture or a subcutaneous antigen extract vaccine of Bordetella bronchiseptica

Healthy dogs with low antibody titer to Bordetella bronchiseptica were vaccinated intranasally with an avirulent live vaccine, subcutaneously with an antigen extract vaccine, or subcutaneously and intranasally with a placebo. Intranasally vaccinated dogs developed B. bronchiseptica-specific IgA titers in nasal secretions that remained at high levels until the end of the study; dogs vaccinated subcutaneously with the antigen extract or placebo did not develop measurable antigen-specific IgA titers in nasal secretions. Dogs were challenged with virulent live B. bronchiseptica 63 days after vaccination. Intranasally vaccinated dogs had significantly lower cough scores (P < or =.0058) and shed significantly fewer challenge organisms (P <.0001) than dogs in either of the other groups. Cough scores of subcutaneously vaccinated dogs were not significantly different from placebo-vaccinated dogs.     

Arbuscular mycorrhizal root colonization and soil P availability are positively related to agrodiversity in Mexican maize polycultures

In Los Tuxtlas, Mexico, the local Popoluca people maintain the traditional management of their maize agroecosystems. However, it is not known whether the loss of agrodiversity over recent decades has affected mycorrhizal populations, nutrient availability, and crop productivity. This study utilized linear mixed effect models to analyze the relationship between agrodiversity (three, six, and greater than or equal to eight cultivated species) and (a) arbuscular mycorrhizal fungi (AMF) inoculum potential, measured as the most probable number (MPN) of propagules and colonization level, (b) nutrient availability, and (c) aboveground maize productivity. We also investigated the relationship between soil nutrient content and inoculum potential. Soil samples were taken before planting, and during flowering, in the 2009 maize cycle. We found that AMF colonization level of maize roots and P availability increased with planted species richness, but that this effect only occurred at the flowering sampling date. Plots with a higher MPN of propagules presented increased C and NO ₃ ^? contents and lower C/N ratio than those with lower MPN of propagules, regardless of agrodiversity. Soils that produced the highest maize root colonization level also featured high P availability and N content. We conclude that decreased agrodiversity in these traditional systems does not significantly affect the soil MPN of propagules, but may have a negative impact on the ability of the mycorrhizal community to colonize maize roots, as well as reducing the availability of P, which is often the most limiting nutrient in tropical soils.     

Soil pH Buffering Capacity and Nitrogen Availability Following Compost Application in a Tropical Acid Soil

Tropical acid soils are highly weathered as they exist under tropical environment with high rainfall and temperature throughout the year, which affects nitrogen availability. Soil organic nitrogen is important in estimating soil nitrogen availability. The combined use of urea and compost in this study was carried out to decrease sole dependence on urea, buffer soil acidification, and reduce nitrogen losses through leaching. Thus, soil buffering capacity, incubation, and organic nitrogen fractionation studies were conducted to determine soil buffering capacity, availability of total nitrogen, organic fractions nitrogen, and inorganic nitrogen in soil after 90 days of incubation following compost. Soil pH, buffering capacity, total nitrogen, organic nitrogen fractions, exchangeable ammonium, and available nitrate were higher in all treatments with compost and combined use of urea and compost. Total hydrolyzable nitrogen, ammonium-nitrogen, (ammonium + amino sugar)-nitrogen, amino sugar-nitrogen, and amino acid-nitrogen were higher in soils with urea and compost suggesting that decomposition of soil organic fractions nitrogen into inorganic nitrogen (ammonium and available nitrate was affected by the addition of urea and compost. Urea can be amended with compost to regulate availability nitrogen in soil for crop use.     

A Thermo-Cavitation Method to Determine Organochlorine Pesticides in Gas and Particle Phases Collected in Polyurethane Foam Used in Passive Air Samplers

Persistent organic pollutants (POPs) are globally dispersed substances considered to have adverse effects on health and ecosystems. Organochlorine pesticides (OCPs) are POPs. OCPs can be collected from the air by passive samplers using polyurethane foam (PUF). POPs in PUF are generally extracted by Soxhlet, considered as reference extraction method. We offer a different methodology approach to extract 16 OCPs from the PUF. This technology extracts, filters, collects the sample and evaporates the solvent, without sample transfer. It is a modification of the ultrasound-assisted extraction micro-scale cell (M-UAE-MSC) previously patented by our group. A full factorial experimental design (2³ centred) was used to optimise the extraction conditions. The most desirable conditions were 50 °C, 40% ultrasound potency and 20-min extraction time, two extractions. OCP recoveries ranged from 67 to 110%. OCP recoveries were equivalent by M-UAE-MSC and Soxhlet techniques. The method was not suitable for five OCPs, and further refinement is needed. Method linearity (r²) was ≥?0.98. Limits of detection were observed between 1.2 and 4.6 ng PUF^?1, while limits of quantification were found between 3.9 and 15.2 ng PUF^?1. The method was applied to determine OCPs collected by PUF passive samplers in Mexico. Endosulfan I was the OCP most frequently observed. The M-UAE-MSC optimised extraction conditions, decreased the extraction time from several hours to less than 1 h and reduced three times the solvent consumption with respect to Soxhlet. This afforded a decrease in the waste generation and a reduction in the OCP-extraction cost up to 80%. The results of this study reveal an efficient and consistent procedure, as well as a simpler, faster, cost-saving and more environmentally friendly method to determine OCPs collected by PUF in passive samplers compared to Soxhlet extraction.     

Amending Chemical Fertilizers with Rice Straw Compost and Clinoptilolite Zeolite and Their Effects on Nitrogen Use Efficiency and Fresh Cob Yield of Zea mays L.

A field study was carried out from April 2014 to August 2014 for two consecutive planting cycles of Zea mays L. on Nyalau Series (Typic Tualemkuts) to determine the short-term effects of co-application of chemical fertilizers, rice straw compost, and clinoptilolite zeolite on (i) ammonium adsorption and desorption, (ii) nitrogen, phosphorus, and potassium uptake and use efficiency, and (iii) yield of Zea mays L. (cobs). Amending urea with rice straw compost and clinoptilolite zeolite improved nitrogen use efficiency because of temporary adsorption and desorption of ammonium on the exchange sites of compost and clinoptilolite zeolite. Combined use of chemical fertilizers, rice straw compost, and clinoptilolite zeolite enhanced uptake of nitrogen, phosphorus, potassium, calcium, and magnesium. Co-application of chemical fertilizers, rice straw compost, and clinoptilolite zeolite can improve the availability of soil nutrients. This approach can also improve nutrient use efficiency and yield of Zea mays L.     

A light and electron microscopic study on abomasal globidiosis in Somali goats

Abomasum from apparently healthy Somali goats with globidiosis showed pin-head sized nodules embedded in the mucosa. The nodules consisted of encapsulated cysts, containing mature or immature schizonts. Glandular atrophy and lymphohistiocytic cell reaction were often found in the vicinity of these cysts. The fine structure of immature and mature cysts is described in details. The mature cysts contained elongated, spindle shaped merozoites (type I) or shorter, ovoidal merozoites (type II). Some mature cysts also had basophilic granular bodies among the merozoites. Type I and type II merozoites were morphologically different from those earlier described in goats.     

东疆沙蜥和密点麻蜥的食性研究

本文对东疆沙蜥和密点麻蜥的食性特征进行了调查研究。结果表明：（１） 这两种蜥蜴的食物组成是小昆虫,主要是鞘翅目、膜翅目和半翅目等对人类有害的昆虫。（２） 食物的充塞度和活动个体数在春季有一个高峰,而在夏季有两个高峰。（３）在食物成分上有季节性变化,在春季主要猎食象鼻虫而夏季主要猎食蚜虫。（４） 它们的有益系数大于其它的两栖类和爬行类动物。