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41.
Macarisin D Cohen L Eick A Rafael G Belausov E Wisniewski M Droby S 《Phytopathology》2007,97(11):1491-1500
ABSTRACT During the infection of citrus fruit by Penicillium digitatum there is little evidence of a host defense response. This suggests that P. digitatum has the ability to suppress host defenses. The current study demonstrates that P. digitatum suppresses a defense-related hydrogen peroxide (H(2)O(2)) burst in host tissue. In contrast, the nonhost pathogen, Penicillium expansum, triggers production of a significant amount of H(2)O(2) in citrus fruit exocarp. Using laser scanning confocal microscopy, we demonstrated that P. digitatum suppressed an elevation in H(2)O(2) up to 42 h after inoculation. Nevertheless, H(2)O(2) levels around wounds inoculated with P. expansum increased by 63-fold above the control. P. digitatum continued to suppress H(2)O(2) production in citrus fruit exocarp up to 66 h postinoculation and H(2)O(2) levels were actually threefold below that of noninoculated controls. In contrast, the H(2)O(2) level was still about 11-fold above the control value in wound sites inoculated with P. expansum. Studies on the effect of organic acids (as pH modulators) on the response of citrus fruit to compatible and noncompatible pathogens indicated that pathogenicity was enhanced only when host-tissue acidification was accompanied by the suppression of H(2)O(2). Additionally, pathogenicity of both P. digitatum and P. expansum on citrus fruit was significantly enhanced by the H(2)O(2)-scavenging enzyme catalase. Based on our study and previous reports regarding the potential involvement of citric acid and catalase in green mold pathogenesis, we suggest that these compounds are strongly associated with the virulence of P. digitatum. 相似文献
42.
Cohen Abigail L. Illan Javier Gutierrez Pfeiffer Vera W. Wohleb Carrie H. Crowder David W. 《Journal of pest science》2022,95(1):315-325
Journal of Pest Science - Long-term monitoring networks that generate data on pest abundance are the foundation of integrated pest management. Monitoring can estimate local risk from pests and... 相似文献
43.
Rebecca Cohen REGAN Robert Michael GOGAL Jr James Perry BARBER Richard Cary TUCKFIELD Elizabeth Wynne HOWERTH Jessica Ann LAWRENCE 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2014,76(12):1563-1568
Loperamide is a peripheral
opiate agonist that can cause apoptosis and G2/M arrest in human cancer cell lines and may
sensitize cells to chemotherapy. The objectives of this study were to investigate the
effects of loperamide on viability, apoptosis and cell cycle kinetics in canine cancer
cells and to establish whether the drug sensitizes cells to doxorubicin. Cell viability
was assessed using Alamar Blue. Cell death and cell cycle were studied using flow
cytometry with 7-Aminoactinomycin-D (7-AAD) and propidium iodide (PI), respectively.
Loperamide decreased cell viability in a dose-dependent fashion and was most effective
against canine osteosarcoma cells. In all cell lines, it induced a dose and time dependent
apoptosis and resulted in accumulation in G0/G1. When co-incubated with doxorubicin,
loperamide induced a synergistic cell kill in canine carcinoma cells. Investigation is
warranted into the role of loperamide in the treatment of canine cancer. 相似文献
44.
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46.
We studied the photostabilizing effect of the isoquinoline alkaloid berberine on the following three model photounstable biocontrol agents: the bacterium Bacillus thuringiensis ( Bt ), the baculovirus Spodoptera littoralis nucleopolyhedrosis virus ( SlNPV ), and the entomopathogenic fungus Beauveria bassiana . The photoprotection provided by berberine was evaluated following various periods of simulated sunlight-UV irradiation. Insecticidal activity of Bt and SlNPV , as well as the rate of B. bassiana spore germination served as parameters for the photostabilizing effects. Only a berberine- Bt complex is effective, as practically no photostabilization was evident by just mixing the two components. The UV-sensitive SlNPV and B. bassiana spores were considerably photostabilzed by berberine. The role of the cationic nature of the berberine molecule and the importance of its absorbance spectrum in photostabilization of biocontrol agents are discussed. 相似文献
47.
M. Cohen E. A. Sartin E. M. Whitley R. D. Whitley A. N. Smith W. R. Brawner R. Henderson E. N. Behrend 《Veterinary and comparative oncology》2003,1(4):232-240
A 6‐year‐old, neutered male domestic shorthair cat was evaluated for a recurrent vaccine‐associated fibrosarcoma. The cat had three excisions of the tumour prior to presentation and was referred for radiation therapy. Ten months following treatment with radiation therapy, the cat was presented again for a cloudy appearance to the eye. An exenteration was performed, and biopsy revealed fibrosarcoma. At the same time, two discrete pulmonary nodules were identified on thoracic radiographs. Two doses of doxorubicin (20 mg/m2) and cyclophosphamide (100 mg/m2) were administered intravenously 3 weeks apart. Despite treatment, the pulmonary nodule doubled in size. This case represents the first antemortem report of ocular metastasis of a vaccine‐associated sarcoma and supports the highly aggressive nature of these tumours. 相似文献
48.
J.L. Hernandez J.G. Besso D.N. Rault A.H. Cohen A. Guionnet D. Begon Y. Ruel 《Veterinary radiology & ultrasound》2003,44(2):196-198
An 8-year-old intact female West Highland White Terrier was examined for anorexia, vomiting, abdominal distension, and mild purulent vulvar discharge. The results of physical examination, laboratory testing, and radiography are presented. Emphysematous pyometra was suspected and confirmed at surgery. Clostridium perfringens was isolated from the uterine lumen. A brief discussion of emphysematous pyometra is presented. 相似文献
49.
The tomato floral system is distinguished from that of Arabidopsis and Antirrhinum in several ways. The shoot is a sympodium, the first flower of the inflorescence is apical and no bracts are formed. We discuss the possible function of genes affecting growth habit and inflorescence development and analyze in detail the unique developmental alterations caused by inhibition or ectopic expression of tomato-specific MADS-box genes. Preliminary analysis of transgenic tomato plants expressing an alien homeotic gene and several different chimeric genes of the MADS-box family is also reported. 相似文献
50.
Phenotypic and genotypic analysis of a commercial cultivar and wild populations of Anemone coronaria
Yonash Nissim Fang Jinggui Shamay Arik Pollak Neta Lavi Uri Cohen Avner 《Euphytica》2004,136(1):51-62
Seven wild populations of Anemonecoronaria were assessed for 11 phenotypic traits, most of them having economic value for the flower industry. The wild populations
were sampled to represent the diversity in habitats, climates, rock and soil types, terrains, and elevations in Israel. AFLP
analysis was carried out on 12 individuals from each of six out of the seven wild populations and for six individuals from
the commercial cultivar ‘Mona-Lisa’. It was found that the Dorot population, which is located in the area bordering the semi-arid
zone at the very end of the species distribution, exhibits extreme and different phenotypes with relatively low variability
compared withthe other wild populations. The other six wild populations, that grow in more favorable geographic and climatic
conditions exhibit phenotypes with larger plants, larger numbers of flowers and less dissected leaves. These populations were
less uniform than that of Dorot. Genetic characterization by AFLP markers revealed a total of 165 bands. The wild populations
exhibit wide variation within-population, with about 80% polymorphic bands and average gene diversity between pairs of about
30%. The Dorot population has the lowest genetic variation and the Megido population the highest. Thus, the phenotypic variation
reflects the genetic variation. The cultivar ‘Mona-Lisa’, as expected, has much lower genetic variation. The Dorot population
and the ‘Mona-Lisa’ cultivar were found to have the largest genetic distances from the other wild populations, and the highest
genetic variation between themselves. Phenetic analysis yielded a dendrogram describing the genetic relatedness of these populations.
This revised version was published online in August 2006 with corrections to the Cover Date. 相似文献