Immunohistochemical analysis for G protein in the olfactory organs of soft-shelled turtle, Pelodiscus sinensis

In turtles, the epithelia lining the upper and lower chambers of the nasal cavity project axons to theventral and dorsal parts of the olfactory bulbs, respectively. In a semi-aquatic soft-shelled turtle,Pelodiscus sinensis, more than 1,000 odorant receptor genes have been found, but it is notknown where they are expressed. In this study, we aimed to clarify the distribution of cells expressing thesegenes in the olfactory organs of soft-shelled turtles. Immunoreactions for the Gαolf, the α subunit of Gprotein coupled to the odorant receptors, were detected on the surface of epithelia lining both the upper andlower chambers of the nasal cavity. The receptor cells in the epithelium of both chambers possessed cilia onthe tip of their dendrites, whereas microvillous, non-ciliated, receptor cells were not found. These datasuggest that the odorant receptor genes are expressed by the ciliated receptor cells in the upper and lowerchamber epithelia. Precise location of the vomeronasal epithelium is not known at present.     

Granulomatous nephritis and meningoencephalomyelitis caused by Halicephalobus gingivalis in a pony gelding

A ten-year-old Shetland pony gelding showed low appetite, ataxia, peculiar swaying, clouding of consciousness, and ultimately died. At necropsy, multiple coalescing granulomatous foci were detected in the kidneys, and small necrotic lesions were found in the cerebellum. Histologic examination of the renal tissue sections revealed extensive granuloma, and Halicephalobus gingivalis-like nematodes were seen. Similar nematodes were found in the granulomatous or necrotic lesions of the renal lymph nodes and cerebellum, and were also frequently detected in cerebrospinal meningovascular lesions. Morphologic features together with partial ribosomal RNA gene sequences of the nematodes in the lesions revealed that they were H. gingivalis. The present results indicated that H. gingivalis caused granulomatous nephritis and meningoencephalomyelitis in this pony gelding.     

Severe hypoplasia of the omasal laminae in a Japanese Black steer with chronic bloat--a case report

An 11-month-old Japanese Black steer with chronic bloat underwent clinical and histological analyses. During the observation period, it showed normal appetite and fecal volume but persistent chronic bloat symptoms. Compared to controls, the steer's feces contained undigested large straws. Necropsy revealed normal rumen, reticulum, and abomasum but a small omasum. The rumen, reticulum, and abomasum mucosa was normal, with well-developed ruminal papillae. However, severe hypoplasia of the omasal laminae was observed along with hypoplasia reticular groove and ruminoreticular fold. The contents of the reticulum, omasum, and abomasums comprised undigested large sized hay particles. The omasum papillae showed no pathological abnormalities. This is a rare case of a steer with chronic bloat probably caused by severe hypoplasia of the omasal laminae.     

Effect of danazol on NK cells and cytokines in the mouse uterus

Danazol, which has been used as a medicine for endometriosis, has a valid effect in pretreatment of patients receiving in vitro fertilization and embryo transfer, although its reproductive mechanism remains unclear. BALB/c mice were subcutaneously injected with danazol for 2 weeks. Blood and uteri were collected and cytokines were assayed. Following danazol treatment, an increase in pregnancy ratio was evident that was accompanied by up-regulation in serum macrophage-colony stimulating factor (M-CSF). RT-PCR analysis revealed that expression of M-CSF and Ly49, a phenotypic marker of natural killer (NK) cells, was up-regulated in the uteri of the danazol-treated mice. In immunohistochemical analysis, M-CSF and Ly49, together with alpha5 integrin, were clearly detected in the endometrium of the danazol-treated mice with very similar pattern of localization. These results suggest that danazol has an effect to promote pregnancy that induces recruitment of NK cells and a concomitant increase in the expression of M-CSF and alpha5 integrin in the uterus.     

Effects of pre-shipping marbofloxacin administration on fever and blood properties in healthy Thoroughbreds transported a long distance

The present study evaluated the effects of single-dose marbofloxacin in protecting horses against fever associated with transportation using 48 healthy Thoroughbreds. All horses were premedicated with interferon-α (0.5 U/kg, sublingually, every 24 hr) for 2 days before transportation and on the day of transportation. Horses were randomly assigned to receive marbofloxacin (2 mg/kg, IV, once; MRFX group), enrofloxacin (5 mg/kg, IV, once; ERFX group) or saline (0.9% NaCl) solution (10 ml, IV, once; control group) ≤1 hr before being transportation. Each group contained 16 horses (8 males, 8 females). Horses were transported 1,210 km using commercial vans over the course of approximately 26 hr. Clinical examinations and hematologic analyses were performed on all horses both before and after transportation. Post-transportation neutrophil to lymphocyte ratios were significantly lower in horses in the MRFX group compared with the control horses. The serum amyloid A levels were significantly lower in horses in the MRFX group and ERFX group compared with the control horses. Regarding the post-transportation rectal temperatures, fever was detected in 0 horses and 1 horse in the MRFX and ERFX groups, respectively, whereas fevers exceeding 39.1°C were detected in 2 horses in the control group. Additionally, the number of essential post-transportation treatments provided by veterinarians was reduced 3-fold in the MRFX and ERFX groups compared with the saline group. MRFX provided ERFX-like protection against fever associated with long-distance transportation, yielding significantly better protection than saline. Administration of MRFX just before transportation deserves a further study for efficacy in preventing horse fever associated with transportation.     

Comparative study between full cell and passive impregnation method of wood preservation for laser incised Douglas fir lumber

In this study, the ability of passive impregnation of Douglas fir (Pseudotsuga menziesii Franco) lumber was compared with a conventional full cell method. With an incising density of 10,000 holes/m² carried out by CO₂ laser, square Douglas fir lumber was treated by both passive impregnation and full cell method for 6 h and 50 min. Different incising densities were also used in passive impregnation to examine the optimum incising density. It was observed that there was no significant difference between these two methods regarding absorption of liquid, moisture content after dipping and penetrated area both at cross and longitudinal sections. However, the value was higher in case of full cell method. Regarding different incising densities, absorption and penetration of liquid was similar for 10,000 and 7,500 holes/m² but it was low for 5,000 holes/m². Therefore, it can be concluded that an incising density of 7,500 holes/m² is the optimum for passive impregnation. The absorption of liquid and its distribution in wood indicates that this non-pressure passive impregnation method can be a good preservation method for impermeable lumber and can be an alternative to full cell method.     

Idling noise from circular saws made of metals with different damping capacities

Circular saw construction using materials with high internal damping has been found to be an effective way to suppress whistling noise during saw idling. A high-damping alloy called Silentalloy (12Cr-3Al-Fe) was previously shown to be an effective material for this purpose. Tests with Silentalloy suggested that a logarithmic decrement of at least 0.07 is needed. Silentalloy does have some disadvantages, such as modest strength, difficult heat treatability, and special order status. The purpose of these experiments was to confirm the general applicability of the minimum effective logarithmic decrement of 0.07 and to find more practical metals for saw-blade construction. Three commercially available metals of different damping capacity were selected. The idling noises of saws made of these metals were compared with those of saws made of typical saw steel and Silentalloy. The minimum logarithmic decrement for whistling-noise suppression was found to be approximately 0.01. On a logarithmic scale, this value is similar to the 0.07 value previously found for a Silentalloy saw. A ferromagnetic steel (20Cr-3A1-Fe) was found to be a useful alternative to Silentalloy from the viewpoint of strength, but the cost of this steel makes it unsuitable for mass production. The success in identifying an effective alternative material to Silentalloy provides encouragement for the identification of other high-damping alloys among the 13Cr-Fe to 18Cr-Fe series without the cost disadvantage of ferromagnetic steel.     

Suppression of rice blast using freeze-killed mycelia of biocontrol fungal candidate MKP5111B

Several applications of the suppressive fungus MKP5111B, isolated from the phylloplane of rice plants, were tested in an effort to control rice blast disease. Three treatments with MKP5111B [living (Std), killed with liquid nitrogen (FR), and autoclaved (AC)] were either sprayed onto rice seedlings or mixed into seed-sown soil. Three weeks after spraying and 4 weeks after the soil application, we introduced Magnaporthe oryzae, the causal agent of rice blast, into our systems. The Std and FR treatments suppressed rice blast, but the AC treatment proved ineffective. Although a suppressive effect was seen on new leaves, no mycelium of MKP5111B was seen. The fungus thus may have induced a systemic resistance in the rice plants. A substance from MKP5111B, such as elicitor molecule(s) are likely responsible for the induced resistance.     

Effects of bisphenol A (BPA) on placentation and survival of the neonates in mice

The effects of bisphenol A (BPA) on placentation have not been fully determined. The aim of this study was to clarify the structural changes of the placenta, abortion rate, and survival of neonates after BPA administration in mice. BPA (10 mg/kg/day) was administered to pregnant mice (BPA mice) subcutaneously from the first day of pregnancy (Day 0) to Day 7 (8 days total). The number of embryos and weights of whole uteri were measured on Days 10 and 12. Morphological changes in the placentae were examined by light microscopy on the corresponding days of pregnancy. The number of neonates was also counted. Survival rates were periodically calculated for neonates from the first day after parturition (P-Day 0) to P-Day 56. The number of embryos and weight of the uterus on Days 10 and 12 were significantly decreased by BPA injection. No notable differences were recognized between the left and right uteri. The proportion of the labyrinthine zone per whole placenta in the BPA mice became lower than that in the controls, and that of the metrial gland was higher in the BPA mice. The intervillous spaces of the placenta were narrower in the BPA mice. Degenerative changes were found in the trophoblastic giant cells and spongiotrophoblast layers of the BPA mice. The number of BPA mouse neonates was drastically decreased within 3 days after birth, and no mice survived after P-Day 56. The results suggest that BPA not only disrupts placental functions and leads to abortion through chronic stimulation of gene expression by binding to DNA but that it also affects the mortality of neonates through indirect exposure of embryos.