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101.
Quantitative trait locus mapping of growth‐related traits in inter‐specific F1 hybrid grouper (Epinephelus fuscoguttatus × E. lanceolatus) in a tropical climate
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Satoshi Kubota Amphai Longloy Arkom Singhabun Wanpen Khammee Kanonkporn Kessuwan Paiboon Bunlipatanon Akiyuki Ozaki Kom Silapajarn Varin Tanasomwang Nobuaki Okamoto Takashi Sakamoto 《Aquaculture Research》2017,48(12):5913-5927
Growth‐related traits are the main target of genetic breeding programmes in grouper aquaculture. We constructed genetic linkage maps for tiger grouper (Epinephelus fuscoguttatus) and giant grouper (E. lanceolatus) using 399 simple sequence repeat markers and performed a quantitative trait locus (QTL) analysis to identify the genomic regions responsible for growth‐related traits in F1 hybrid grouper (E. fuscoguttatus × E. lanceolatus). The tiger grouper (female) linkage map contained 330 markers assigned to 24 linkage groups (LGs) and spanned 1,202.0 cM. The giant grouper (male) linkage map contained 231 markers distributed in 24 LGs and spanned 953.7 cM. Six QTLs affecting growth‐related traits with 5% genome‐wide significance were detected on different LGs. Four QTLs were identified for total length and body weight on Efu_LG8, 10, 13 and 19 on the tiger grouper map, which explained 6.6%–12.0% of the phenotypic variance. An epistatic QTL with a reciprocal association was observed between Efu_LG8 and 10. Two QTLs were identified for body weight on Ela_LG3 and 10 on the giant grouper map, which explained 6.9% of the phenotypic variance. Two‐way analysis of variance indicated that the QTL on Efu_LG13 interacts with the QTLs on Ela_LG3 and 10 with large effects on body weight. Furthermore, these six QTLs showed different features among the winter, summer and rainy seasons, suggesting that environmental factors and fish age affected these QTLs. These findings will be useful to understand the genetic structure of growth and conduct genetic breeding in grouper species. 相似文献
102.
Chow Seinen Suzuki Nobuaki Nakamura Yoji Yasuike Motoshige Saitoh Kenji Yanagimoto Takashi 《International Aquatic Research》2017,9(1):53-59
International Aquatic Research - Nucleotide sequences of four distinct calmodulin genes (designated as CaM-A to -D) of the Pacific bluefin tuna (Thunnus orientalis) were compared. Nucleotide... 相似文献
103.
The root-associated fungus LtVB3 was formerly reported as a potential, new biocontrol agent of Verticillium yellows of Chinese
cabbage and Fusarium wilt of tomato. According to molecular phylogenetic analysis of the ITS 1-5.8S rDNA-ITS 2 gene regions
and morphological characteristics, LtVB3 is here identified as Meliniomyces
variabilis, a recently described species [formerly called “Variable White Taxon” (VWT)] in a new genus erected to accommodate sterile
fungi with phylogenetic affinities to Rhizoscyphus
ericae, an ericoid mycorrhizal fungus. In vitro inoculation experiments showed that LtVB3 colonized the roots of torch azalea and
eucalyptus; however, growth was enhanced only in azalea but not in eucalyptus. Hyphae of M.
variabilis formed intracellular structures typical of ericoid mycorrhizas in epidermal cells of azalea roots but did not produce any
typical ectomycorrhizal structures with or within root cells of either host. In LtVB3-treated eucalyptus roots, some cells
in the epidermal and cortical layers had wall appositions and thickenings, which appeared to restrict fungal growth. 相似文献
104.
Kenichi SASAKI Takamitsu TSUKAHARA Osamu TAIRA Kotaro TSUCHIYA Mitsugi ITOH Kazunari USHIDA 《Animal Science Journal》2010,81(1):135-141
To investigate the transition in concentration of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) and antibody for these viruses in serum, serum samples were collected from 29 pigs on weaning day and at 7, 14, 21, 28, 53, 84, and 120 days after weaning. The concentration of circulated PRRSV and PCV2 in serum was measured by real-time RT-PCR and real-time PCR, respectively. The specific antibody for PRRSV and PCV2 was measured using ELISA. PRRSV was not detected on 0 days post-weaning (dpw). The specific antibody for PRRSV began to increase as the concentration of PRRSV in serum increased, and the level of PRRSV then tended to decrease. PCV2 was detected in 12 of 28 pigs on 0 dpw. The concentration of PCV2 and the specific antibody for PCV2 showed a similar tendency to those of PRRSV. The correlation analysis suggests that a decline in the daily weight gain coincided with an increase in the PRRSV concentration. Pigs with a higher antibody titer against PRRSV or PCV2 on 0 dpw showed the lower level of PRRSV or PCV2, respectively. 相似文献
105.
Hiromichi Mitamura Yasushi Mitsunaga Nobuaki Arai Yukiko Yamagishi Metha Khachaphichat Thavee Viputhanumas 《Fisheries Science》2008,74(4):787-795
Abstract: The horizontal and vertical movements of eight immature hatchery-reared Mekong giant catfish Pangasianodon gigas were monitored using acoustic telemetry in Mae Peum Reservoir, Thailand between 2003 and 2004. The fish were monitored for between eight days and more than 9 months. All of the fish moved over the entire reservoir within approximately 40 days after release. Subsequently, the home range became small approximately 40 days after release. The fish preferred deep areas in the reservoir. The fish displayed diel horizontal and vertical movement patterns. The fish showed larger-scale horizontal movement during the daytime than at night. The fish repeatedly showed active vertical movement during the day, whilst there was little variation in swimming depth at night. Our results imply that the vertical movements of the fish were related to the environmental condition of the reservoir. 相似文献
106.
Hiromichi Mitamura Yasushi Mitsunaga Nobuaki Arai Thavee Viputhanumas 《Fisheries Science》2008,74(5):1034-1039
Twenty-eight immature hatchery-reared Mekong giant catfish Pangasianodon gigas tagged with acoustic transmitters were released in the Mekong River, Thailand from 2002 to 2004. Twenty-four and four fish
were tagged with normal transmitters and pressure-sensitive transmitters, respectively. Five to seven automated monitoring
receivers were used for monitoring the post-release movements. The tagged catfish could be detected for up to 97 days, the
first detection taking place at the release point, where the fish remained for several days. Sixteen tagged fish (57%) were
not detected at any later point. These fish may have passed along the opposite (Laos) side of the river without notice because
the width of the river was larger than the detection range of the transmitter. The remaining 12 tagged fish (43%) could be
detected by the receivers installed, excluding the release point receiver. Of these 12 tagged fish, six showed long-distance
(30–80 km) upstream movements and one long-distance (50 km) downstream movement. These seven fish (25%) were detected only
during the daytime, suggesting that the Mekong giant catfish is diurnal. 相似文献
107.
Toshihiro Wada Kyoichi Kamiyama Hiromichi Mitamura Nobuaki Arai 《Fisheries Science》2017,83(4):573-585
To elucidate the post-release movement and emigration of juvenile spotted halibut Verasper variegatus, ten hatchery-reared age-0 individuals (10 months old, 17.0–19.7 cm total length) surgically implanted with acoustic transmitters were released near the center of Matsukawa-ura, a shallow brackish lagoon, on 25 November 2009 (water temperature 13.8 °C). They were monitored by ten acoustic receivers for 5 months. Of ten individuals, eight left the release site during December–March. Three of these emigrated to the outer ocean on 17 and 29 January and 30 March 2010, respectively. Juveniles probably started wintering migration in December and January when water temperatures decreased considerably in the lagoon (mean 9.5 and 6.0 °C, respectively). They stopped their migration in the coldest month, February (mean 5.7 °C), and restarted it in March when water temperatures frequently exceeded a plausible threshold for movement (≥6 °C). Statistical analyses revealed that the fish started migration significantly more frequently at nighttime. The migration track of an individual recorded from 11 to 30 March showed gradual nocturnal movements and a slow migration speed (estimated maximum speed 2.2 m/min). Our results revealed that water temperature primarily governed the seasonal timing of nocturnal migratory movements of juvenile spotted halibut. 相似文献
108.
Nobuaki Nanjo Tetsuya Takatsu Kazuo Imura Kingo Itoh Yuuhei Takeya Toyomi Takahashi 《Fisheries Science》2017,83(2):199-214
To clarify the recruitment process of sand lance Ammodytes sp., we investigated larval condition factor, relative gut fullness (%GF), prey abundance and oceanographic structure in Mutsu Bay, Japan, during 1999–2001. Ammodytes sp. larvae, which were collected by horizontal hauls of Motoda nets and a ring net at depths of 1, 10, 20, 30 and 40 m, were mainly distributed at 10–30 m. Larvae at the first feeding time until 12 mm in body length (BL) fed predominantly on copepod nauplii, whereas large larvae with BL of 12.1–14.0 mm fed on a mixture of copepod nauplii, copepodites and appendicularians from late February to April. A path analysis showed that difference in water density between 35- and 5-m depths negatively affected naupliar abundance at 10–30-m depth (standardised path coefficient β = ?0.71, p = 0.005 for 3.3–8.0-mm BL larvae and β = ?0.78, p < 0.001 for 8.1–12.0-mm BL larvae). Naupliar abundance positively affected the %GF of Ammodytes sp. larvae (β = 0.75, p < 0.001 for 3.3–8.0-mm BL larvae and β = 0.66, p < 0.001 for 8.1–12.0-mm BL larvae), whereas it was negatively affected by water temperature (β = ?0.45, p = 0.008 for 3.3–8.0-mm BL larvae and β = ?0.56, p = 0.002 for 8.1–12.0-mm BL larvae), and the temperature effect was weak compared with that of naupliar abundance. In turn, %GF positively affected larval somatic weight (β = 0.91, p < 0.001 for 6.0-mm BL larvae and β = 0.70, p = 0.005 for 10.0-mm BL larvae). The recruitment failure in 1999 was likely caused by a reduced condition factor, which resulted from low naupliar abundance. In contrast, the abundance of nauplii and Oithona similis copepodites was high in 2000 and 2001. It is possible that the higher recruitment success in 2001 was because of the higher water temperatures in Mutsu Bay, sustaining faster growth of the larvae than in 2000 under the high-prey abundance conditions. 相似文献
109.
Yuuki Kawabata Kimio Asami Masato Kobayashi Taku Sato Koichi Okuzawa Hideaki Yamada Kenzo Yoseda Nobuaki Arai 《Fisheries Science》2011,77(1):79-85
A critical component of many releasing projects is the identification and subsequent implementation of optimal release strategies
that can decrease post-release predation mortalities. We performed laboratory experiments to investigate whether acclimation
to shelters affects the post-release survival of hatchery-reared black-spot tuskfish Choerodon schoenleinii in the presence of a reef-resident predator, the white-streaked grouper Epinephelus ongus. Tuskfish were exposed to groupers under three different experimental conditions/treatments: (1) acclimation of fish to shelters
prior to their exposure to groupers; (2) no acclimation of fish to shelters, but with shelters available during their exposure
to groupers; (3) fish not acclimated to shelters and no shelters available during their exposure to groupers. Tuskfish that
were acclimated to shelters utilized shelters more frequently than did non-acclimated fish, and the survival rate of acclimated
fish was higher than those of fish in the other treatments. These results suggest that pre-release acclimation to shelters
improves the post-release survival of hatchery-reared black-spot tuskfish. 相似文献
110.
Keiichi?UchidaEmail author Nobuaki?Arai Kazuyuki?Moriya Yoshinori?Miyamoto Toshiharu?Kakihara Tadashi?Tokai 《Fisheries Science》2005,71(5):992-1002
ABSTRACT: In this study, we developed a new automatic system, comprised of a radio frequency identification (RFID) system and global positioning system (GPS), for monitoring fishing effort, and effectiveness of effort, in fisheries using many pieces of fishing gear, such as hooks of longlines. The outline of this system is as follows. A single RFID tag with an identification (ID) number is attached to each piece of fishing gear. The RFID tag on the fishing gear passes the antenna of the RFID reader before being shot into the sea and after being hauled up from the sea. Data on the time and geographic location are measured by the GPS and recorded along with the ID number in a personal computer (PC). When a fish is caught, it is brought close to the fish-sorting table. Then, the second antenna of the RFID reader set at the side checks for a catch in the gear. The advantage of this system is that the fishing operation data can be collected without interfering with the fishermen's usual work. The prototype of the system was tested at a conger-eel tube fishery in Tokyo Bay and was able to record data on the time, the location and the catch of individual conger-eel tubes successfully. 相似文献