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31.
Mahmoud A.O. Dawood Mahmoud S. Gewaily Ali A. Soliman Mustafa Shukry Asem A. Amer Elsayed M. Younis Abdel-Wahab A. Abdel-Warith Hien Van Doan Adel H. Saad Mohamed Aboubakr Hany M.R. Abdel-Latif Sabreen E. Fadl 《Marine drugs》2020,18(12)
Marine-derived substances are known for their beneficial influences on aquatic animals’ performances and are recommended to improve intestinal health, immunity, and anti-oxidative status. The present study investigates the role of chitosan nanoparticles on the intestinal histo-morphometrical features in association with the health and immune response of Grey Mullet (Liza ramada). Chitosan nanoparticles are included in the diets at 0, 0.5, 1, and 2 g/kg and introduced to fish in a successive feeding trial for eight weeks. The final body weight (FBW), weight gain (WG), and specific growth rate (SGR) parameters are significantly increased while feed conversion ratio (FCR) decreases by chitosan nanoparticles compared to the control (p < 0.05). The morphometric analysis of the intestines reveals a significant improvement in villus height, villus width, and the number of goblet cells in chitosan-treated groups in a dose-dependent manner. Additionally, there is a positive correlation between the thickness of the enterocyte brush border and the chitosan dose, referring to an increasing absorptive activity. Histologically, the intestinal wall of Grey Mullet consists of four layers; mucosa, sub-mucosa, tunica muscularis (muscular layers), and serosa. The histological examination of the L. ramada intestine shows a normal histo-morphology. The epithelial layer of intestinal mucosa is thrown into elongated finger-like projections, the intestinal villi. The values of hemoglobin, hematocrit, red blood cells (RBCs), total protein (TP), albumin, and globulin are significantly increased in fish fed 1, and 2 g/kg of chitosan nanoparticles compared to fish fed 0 and 0.5 g/kg (p < 0.05). The highest levels of TP and albumin are observed in fish fed 1 g/kg diet (p < 0.05). The lysozyme activity and phagocytic index are significantly enhanced by feeding chitosan nanoparticles at 0.5, 1, and 2 g/kg, whereas the phagocytic activity is improved in fish fed 1 and 2 g/kg (p < 0.05). The highest lysozyme activity and phagocytic index are observed in fish fed 1 g/kg. SOD is significantly activated by feeding chitosan nanoparticles at 1 g/kg. Simultaneously, glutathione peroxidase (GPx) and catalase (CAT) activities also are enhanced by feeding chitosan at 1 and 2 g/kg, compared to fish fed 0 and 0.5 g/kg (p < 0.05). The highest GPx and CAT activities are observed in fish fed 1 g/kg (p < 0.05). Conversely, the malondialdehyde (MDA) levels are decreased by feeding chitosan at 1 and 2 g/kg, with the lowest being in fish fed 1 g/kg (p < 0.05). To summarize, the results elucidate that L. ramada fed dietary chitosan nanoparticles have a marked growth rate, immune response, and anti-oxidative response. These improvements are attributed to the potential role of chitosan nanoparticles in enhancing intestinal histo-morphometry and intestinal health. These results soundly support the possibility of using chitosan nanoparticles at 1–2 g/kg as a feasible functional supplement for aquatic animals. 相似文献
32.
Marilia Barreca Virginia Span Alessandra Montalbano Mercedes Cueto Ana R. Díaz Marrero Irem Deniz Ayegül Erdoan Lada Luki Bilela Corentin Moulin Elisabeth Taffin-de-Givenchy Filippo Spriano Giuseppe Perale Mohamed Mehiri Ana Rotter Olivier P. Thomas Paola Barraja Susana P. Gaudêncio Francesco Bertoni 《Marine drugs》2020,18(12)
The marine environment is a rich source of biologically active molecules for the treatment of human diseases, especially cancer. The adaptation to unique environmental conditions led marine organisms to evolve different pathways than their terrestrial counterparts, thus producing unique chemicals with a broad diversity and complexity. So far, more than 36,000 compounds have been isolated from marine micro- and macro-organisms including but not limited to fungi, bacteria, microalgae, macroalgae, sponges, corals, mollusks and tunicates, with hundreds of new marine natural products (MNPs) being discovered every year. Marine-based pharmaceuticals have started to impact modern pharmacology and different anti-cancer drugs derived from marine compounds have been approved for clinical use, such as: cytarabine, vidarabine, nelarabine (prodrug of ara-G), fludarabine phosphate (pro-drug of ara-A), trabectedin, eribulin mesylate, brentuximab vedotin, polatuzumab vedotin, enfortumab vedotin, belantamab mafodotin, plitidepsin, and lurbinectedin. This review focuses on the bioactive molecules derived from the marine environment with anticancer activity, discussing their families, origin, structural features and therapeutic use. 相似文献
33.
Wei-wei ZHANG Xiao-ying ZHAN Shu-xiang ZHANG Khalid Hamdan Mohamed Ibrahima Ming-gang XU 《农业科学学报》2019,18(3):667-676
The concentration of soil Olsen-P is rapidly increasing in many parts of China, where P budget(P input minus P output) is the main factor influencing soil Olsen-P. Understanding the relationship between soil Olsen-P and P budget is useful in estimating soil Olsen-P content and conducting P management strategies. To address this, a long-term experiment(1991–2011) was performed on a fluvo-aquic soil in Beijing, China, where seven fertilization treatments were used to study the response of soil Olsen-P to P budget. The results showed that the relationship between the decrease in soil Olsen-P and P deficit could be simulated by a simple linear model. In treatments without P fertilization(CK, N, and NK), soil Olsen-P decreased by 2.4, 1.9, and 1.4 mg kg~(–1) for every 100 kg ha~(–1) of P deficit, respectively. Under conditions of P addition, the relationship between the increase in soil Olsen-P and P surplus could be divided into two stages. When P surplus was lower than the range of 729–884 kg ha~(–1), soil Olsen-P fluctuated over the course of the experimental period with chemical fertilizers(NP and NPK), and increased by 5.0 and 2.0 mg kg~(–1), respectively, when treated with chemical fertilizers combined with manure(NPKM and 1.5 NPKM) for every 100 kg ha~(–1) of P surplus. When P surplus was higher than the range of 729–884 kg ha~(–1), soil Olsen-P increased by 49.0 and 37.0 mg kg~(–1) in NPKM and 1.5 NPKM treatments, respectively, for every 100 kg ha~(–1) P surplus. The relationship between the increase in soil Olsen-P and P surplus could be simulated by two-segment linear models. The cumulative P budget at the turning point was defined as the "storage threshold" of a fluvo-aquic soil in Beijing, and the storage thresholds under NPKM and 1.5 NPKM were 729 and 884 kg ha~(–1)P for more adsorption sites. According to the critical soil P values(CPVs) and the relationship between soil Olsen-P and P budget, the quantity of P fertilizers for winter wheat could be increased and that of summer maize could be decreased based on the results of treatments in chemical fertilization. Additionally, when chemical fertilizers are combined with manures(NPKM and 1.5 NPKM), it could take approximately 9–11 years for soil Olsen-P to decrease to the critical soil P values of crops grown in the absence of P fertilizer. 相似文献
34.
Journal of Crop Science and Biotechnology - Priming is a safe, easy, and effective way to increase plant tolerance against stress. This research aims to study the metabolic status of the... 相似文献
35.
Abdelhaleem Fahmy Salah Amin Ahmed Mostafa Basiouny Mohamed Elsayed Ibraheem Heba Fathy 《Journal of Soils and Sediments》2020,20(3):1742-1753
Journal of Soils and Sediments - Bedload transport discharge is important in river engineering and morphodynamics. The Meyer-Peter and Müller (MPM) equation for determining bedload transport... 相似文献
36.
37.
While the respiratory quotient (R.Q.) of Rhinomugil corsula in air-saturated water remains near unity at all levels of random activity, the ammonia quotient (A.Q. = volume or mole: mole relation of ammonia excreted to oxygen consumed) is higher at lower levels of activity, indicating relatively higher protein utilization in less active fish. The mean routine R.Q. values of R. corsula in air-saturated water at temperatures of 30 and 35°C are 0.91 and 0.95 respectively. Assuming that the major sources of ammonia excreted are certain predominant free amino acids, an estimate of caloric break-up of routine meta-bolism of R. corsula suggests that the fish is completely aerobic in air-saturated water and that it derives 14, 44 and 42% of energy from proteins, carbohydrates and fats respectively from a total of 567 cal/kg fish/h at 30°C (15, 55 and 30% at 35°C). At oxygen concentrations below air saturation, R.Q. and A.Q. values of R. corsula increase with decrease in ambient oxygen indicating an increase in anaerobic metabolism and protein utilization. The correlation between the trends of R.Q. and A.Q. levels are similar to that observed earlier in Tilapia mossambica. During hypoxia (1.6 mg O2/l at 30°C) R. corsula derives about half its energy for sustenance and activity anaerobically. The mullet does not appear to accumulate an oxygen debt during hypoxia. 相似文献
38.
Fahd M. Abdelkarem Mohamed E. Abouelela Mohamed R. Kamel Alaa M. Nafady Ahmed E. Allam Iman A. M. Abdel-Rahman Ahmad Almatroudi Faris Alrumaihi Khaled S. Allemailem Hamdy K. Assaf 《Marine drugs》2022,20(2)
Gorgostane steroids are isolated from marine organisms and consist of 30 carbon atoms with a characteristic cyclopropane moiety. From the pioneering results to the end of 2021, isolation, biosynthesis, and structural elucidation using 13C-NMR will be used. Overall, 75 compounds are categorized into five major groups: gorgost-5-ene, 5,6-epoxygorgostane, 5,6-dihydroxygorgostane, 9,11-secogorgostane, and 23-demethylgorgostane, in addition to miscellaneous gorgostane. The structural diversity, selectivity for marine organisms, and biological effects of gorgostane steroids have generated considerable interest in the field of drug discovery research. 相似文献
39.
Relative Prevalence of Antibiotic Resistance among Heterotrophic Bacteria from Natural and Culture Environments of Freshwater Prawn,Macrobrachium Rosenbergii
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Abdulla A. Mohamed Hatha A. Deborah Gnana Selvam Ambatt P Thomas 《Journal of the World Aquaculture Society》2016,47(4):470-480
As antibiotic residues and multidrug‐resistant (MDR) bacteria are of increasing concern to those involved in the seafood industry, there is an intense international focus to monitor MDR bacteria in seafood. There is also a trend to source seafood from organically raised farms in order to reduce antibiotic usage in culture operations. Hence this study was conducted to compare the antibiotic resistance among bacteria associated with various samples from the natural and culture environment of Macrobrachium rosenbergii. Samples from the natural environment included water, sediment, eggs, larvae, postlarvae (PL), feed items, and gut samples of M. rosenbergii and those from the culture environment were water, sediment, eggs, feed pellets, and gut samples of M. rosenbergii. Bacteria from water samples of natural and culture environments were frequently resistant to ampicillin, erythromycin, and penicillin, while more than 50% of the strains from sediment were resistant to erythromycin, nalidixic acid, and penicillin. Bacterial isolates from commercial feed had a relatively high degree of antibiotic resistance. Interestingly, occurrence of multiple drug resistance was higher among the bacteria associated with the samples from the natural environment of M. rosenbergii, pointing to more favorable selection pressure for drug resistant mutants in the natural environment. 相似文献
40.
Helena Khatoon Sanjoy Banerjee Fatimah Md Yusoff Mohamed Shariff 《Aquaculture Research》2010,41(9):1348-1355
Marine periphytic cyanobacteria and diatoms have been examined as a potential source of feed supplement for rearing aquatic larvae in the aquaculture industry. Culture of the periphytic diatom Amphora sp., Navicula sp., Cymbella sp. and the cyanobacteria Oscillatoria sp. at different salinities showed significant changes in biomass and specific growth rates. Diatoms growth was significantly higher at 35 g L−1, while for cyanobacteria growth was better at 25 g L−1. Significantly higher levels of protein and lipid were found in diatoms at low salinities (15–25 g L−1) and an increase in carbohydrate at high salinities (30–35 g L−1). Conversely, cyanobacteria showed a significantly higher lipid content at 30–35 g L−1 compared with other salinity levels but no significant changes were observed in the protein and carbohydrate contents at different salinity levels. The present findings can be taken into consideration when culturing marine periphytic Amphora sp., Navicula sp., Cymbella sp. and Oscillatoria sp. to provide appropriate levels of protein, lipid and carbohydrate as feed supplement as well as for bioremediation in aquaculture. 相似文献