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排序方式: 共有304条查询结果,搜索用时 31 毫秒
31.
El Tigani A. Asil Sulieman M. El Sanousi Ahmed Gameel Haytham El Beir Maha Fathelrahman Nasir M. Terab Magzoub A. Muaz Mohamed E. Hamid 《Tropical animal health and production》2013,45(2):469-472
Bovine tuberculosis (BTB) is a widespread zoonosis in developing countries but has received little attention in many sub-Saharan African countries including Sudan and particularly in some parts such as Darfur states. This study aimed to detect bovine tuberculosis among caseous materials of cattle slaughtered in abattoirs in South Darfur State, Sudan by using microscopic and PCR-based methods. The study was a cross-sectional abattoir-based study which examined a total of 6,680 bovine carcasses for caseous lesions in South Darfur State between 2007 and 2009. Collected specimens were examined for the presence of acid-fast bacilli (AFB) by using microscopic and culture techniques. Isolated mycobacteria were identified by selected conventional cultural and biochemical tests in comparison to a single tube multiplex PCR (m-PCR) assay which detect Mycobacterium bovis-specific 168-bp amplicons. Of the total 6,680 slaughtered cattle examined in South Darfur, 400 (6 %) showed caseations restricted to lymph nodes (86.8 %) or generalized (13.2 %). Bovine tuberculosis was diagnosed in 12 (0.18 %), bovine farcy in 59 (0.88 %), unidentified mycobacteria in 6 (0.09 %), and missed or contaminated cultures in 7 (0.1 %). Out of 18 cultures with nonbranching acid-fast rods, 12 amplified unique 168-bp sequence specific for M. bovis and subsequently confirmed as M. bovis. With the exception of the reference M. tuberculosis strains, none of the remaining AFB amplified the 337-bp amplicon specific for M. tuberculosis. It could be concluded that bovine tuberculosis is prevalent among cattle in South Darfur representing 4.5 % from all slaughtered cattle with caseous lesions. The study sustains microscopy as a useful and accessible technique for detecting AFB. m-PCR assay proved to be valuable for confirmation of BTB and its differentiation from other related mycobacteriosis, notably bovine farcy. 相似文献
32.
Saleh K. Majeed 《The Veterinary quarterly》2013,33(2):162-164
Summary Histological examination of 753 Beagle dogs showed renal foci of calcification in 27.9% of males and 21.5% of females. In contrast, stomach calcification was observed in 6.2% of males and 5.2% of females examined. It is suggested that the focal calcification seen in both organs was possibly of dietary origin and may have been related to the phosphate content. 相似文献
33.
Fuad Ameen Sami A. AlYahya Saleh AlNadhari Hadi Alasmari Fahad Alhoshani Milton Wainwright 《Archives of Agronomy and Soil Science》2019,65(10):1446-1459
Desert soils are infertile, and the ability to improve them by P-fertilization is limited by the solubility of phosphate. We aimed to understand the function of phosphate solubilizing bacteria and the mechanisms behind phosphate solubilization in desert soils. Vegetated and barren desert soils, mine spoil and a fertile temperate grassland loam were sampled. Bacteria and fungi were isolated and identified, and their phosphate-solubilizing abilities were measured in vitro. The release of plant available PO4, SO4, NO3 and NH4 from desert soils did not compare with that of a grassland soil. Desert soils had substantially lower solubilization than grassland, 162 and 99–121 µg PO4-P g?1 dry soil, respectively. Phosphate-solubilizing bacteria and fungi were inhabiting the soils. Si addition increased phosphate solubilization of fungi by 50%. The isolated microbes were shown, using 31P nuclear magnetic resonance (NMR) analysis, to rapidly take-up both intracellular and extracellular phosphate during the phosphate solubilizing process. Desert soil had potentially active microbial populations that are capable to solubilize inorganic phosphorus; S and Si as the limiting factors. Acidification as the main mechanism to solubilize mineral phosphate was not as evident in our desert soils as in former studies dealing more fertile soils. 相似文献
34.
Nasir L 《Veterinary journal (London, England : 1997)》2008,175(2):155-163
In recent years in human oncology the enzyme telomerase has emerged as an ideal target for cancer therapy. This has led to the assessment of telomerase in cancers in companion animals, mainly dogs and these studies confirm that in dogs, like humans, telomere maintenance by telomerase is the primary mechanism by which cancer cells overcome their mortality and extend their lifespan. This review aims to provide an introduction to the biology of telomeres and telomerase and to discuss some of the telomere/telomerase directed therapeutic methodologies currently under development which may be of benefit to the canine cancer patient. 相似文献
35.
The objectives of this study were to investigate the effects of flaxseeds and/or fenugreek seeds as a source of phytoestrogens in laying feed on productive performance, egg quality, yolk fatty acids, plasma profile and antioxidative status in aged laying hens. A total of 120 Bovans Brown hens at 65 weeks of age were housed individually in laying cages in an open‐sided building under a 16‐hr light: 8‐hr dark lighting schedule. Hens were randomly divided into four experimental treatments (n = 30 each): (a) control (basal diet without any supplementation); (b) 1 g/kg of flaxseeds; (c) 1 g/kg of fenugreek seeds; and (d) combination of 1 g/kg flaxseeds and 1 g/kg fenugreek seeds from 65 to 71 weeks of age. Hen‐day egg production and egg mass were significantly increased by dietary supplementation of flaxseeds and/or fenugreek seeds, while feed intake was not affected; thus, feed conversion ratio was improved (p < 0.05). Weights of egg, albumen, yolk and shell were increased by the dietary treatments. Shell thickness and yolk colour were improved by supplementation of flaxseeds and/or fenugreek seeds. Yolk crude protein was not significantly affected by treatments; however, crude fat and calcium contents in yolk were increased and egg yolk cholesterol content was decreased. Interestingly, dietary supplementation of flaxseeds, fenugreek seeds and their combination had a significant positive effect on plasma luteinizing hormone, follicle‐stimulating hormone, oestradiol‐17β and triglycerides. Inclusion of flaxseeds and/or fenugreek seeds in the diets enhanced the serum glutathione peroxidase and superoxide dismutase activities and reduced the malondialdehyde and total cholesterol concentrations in plasma. Dietary supplementation of flaxseeds and/or fenugreek seeds increased α‐linolenic and linoleic acids in egg yolk. It could be concluded that inclusion of mixed sources of phytoestrogens in diets improved laying performance, egg quality, the antioxidative status, hormonal profile and steroidogenesis in aged laying hens. 相似文献
36.
The importance of dietary HUFA for meagre larvae (Argyrosomus regius; Asso, 1801) and its relation with antioxidant vitamins E and C
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Najlae El Kertaoui Carmen María Hernández‐Cruz Daniel Montero María José Caballero Reda Saleh Juan Manuel Afonso Marisol Izquierdo 《Aquaculture Research》2017,48(2):419-433
Despite the interest of meagre (Argyrosomus regius) as a fast‐growing candidate for Mediterranean aquaculture diversification, there is a lack of information on nutrition along larval development. Importance of highly unsaturated fatty acids (HUFA) and the antioxidant vitamins E and vitamin C has not been investigated yet in this species. Six diets with two levels of HUFA (0.4% and 3% dw), two of vitamin E (1500 and 3000 mg kg?1) and two of vitamin C (1800 and 3600 mg kg?1) were fed to 15 dah meagre larvae. Larval growth in total length and dry body weight was significantly lowest in larvae fed diet 0.4/150/180 and showed few lipid droplets in enterocytes and hepatocytes and lower HUFA contents than the initial larvae. Increase in dietary HUFA up to 3%, significantly improved larval growth and lipid absorption and deposition. Besides, among fish fed 3% HUFA, increase in vitamin E and vitamin C significantly improved body weight, as well as total lipid, 22:6n‐3 and n‐3 fatty acids contents in the larvae. Thus, the results showed that 0.4% dietary HUFA is not enough to cover the essential fatty acid requirements of larval meagre and a high HUFA requirement in weaning diets is foreseen for this species. Besides, the results also pointed out the importance of dietary vitamin E and C to protect these essential fatty acids from oxidation, increase their contents in the larvae and promote growth, suggesting high vitamin E and C requirements in meagre larvae (higher than 1500 and 1800 mg kg?1 for vitamin E and vitamin C respectively). 相似文献
37.
Muhammad M. Mackeen Abdul M. Ali Mohd A. Abdullah Rozita M. Nasir Nashriyah B. Mat Abdul R. Razak Kazuyoshi Kawazu 《Pest management science》1997,51(2):165-170
Methanolic extracts of 79 Malaysian plants representing 42 families were assessed for antinematodal activity against Bursaphelenchus xylophilus using a fungal-feeding assay. Extracts of 27 plants from 19 families showed antinematodal activity, while 52 species were inactive. Five extracts (Sauropus androgynus, Eugenia polyantha, Areca catechu, Piper betle and Piper nigrum) exhibited very strong activity against Bursaphelenchus xylophilus at a minimum effective dose (MED) of 0·625 mg per ball. Strong antinematodal activity (MED: 1·25–2·5 mg per ball) was shown by the extracts of Spondias cyntherea, Codiageum variegatum, Euodia glabra and Cicca acida. Eleven extracts (Carica papaya, Ipomoea aquatica, Ocimum basilicum, Leea gigantea, Pithecellobium jiringa, Crypteronia paniculata, Myristica fragrans, Murraya koenigii, Leucaena leucocephala, Melastoma malabathricum and Morinda citrifolia) demonstrated moderate activity between MED of 5 and 10 mg per ball, and weak activity was observed in seven extracts (Ipomoea batatas, Cymbopogon citratus, Garcinia atroviridis, Psophocarpus tetragonolobus, Tamarindus indica, Allium odorum and Stenochalaena palustris). © 1997 SCI 相似文献
38.
39.
Suhaila Mohamed Suzana Saka Saleh H. El-Sharkawy Abdul Manaf Ali Sepiah Muid 《Pest management science》1996,47(3):259-264
Ethanolic extracts of 58 Malaysian plants belonging to 24 different families were screened for antifungal activity against seven plant pathogens using the filter paper disc diffusion technique. Two varieties of Piper betle, showed strong activity against all the pathogens tested (Colletotrichum capsici, Fusarium pallidoroseum, Botryodiplodia theobromae, Alternaria alternata, Penicillium citrinum, Phomopsis caricae-papayae and Aspergillus niger), with inhibition diameters significantly (P<0·01) bigger than 2·5 mg ml−1 prochloraz or 10 mg ml−1 clotrimazole. The minimum inhibitory concentrations of the ethanolic extracts of P. betle against these plant pathogens ranged between 0·01 mg ml−1 and 1 mg ml−1. Thirty-four other plants (Kucing gala, Limau batik, Bertholletia excelsa, Bixa orellana, Caesalpinia pulcherrima, Cerbera odollam (fruits and leaves), Colocasia gigantea, Curcuma domestica, Curcuma manga, Derris eliptica, Elephantopus scaber, Eleusine indica, Eugenia polyantha, Euphorbia hirta, Euphorbia tirucalli, Gardenia florida, Hedyotis auricularia, Hibiscus rosa-sinensis, Juniperus chinensis (three varieties), Lawsonia inermis, Lecythis ollaria, Mentha arvensis, Mimusops elengi, Ocimum sanctum, Phyllanthus niruri, Piper nigrum, Piperomia pellucida, Pedilanthus tithymaloides, Polygonum minus, Spondias dulcis, Solanum nigrum, Tinospora tuberculata) showed selective antifungal activity, while 21 species were inactive. 相似文献
40.