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161.
Naoto Yoshida 《Plant pathology》2022,71(2):361-372
Setophoma terrestris, a ubiquitous inhabitant of soil, causes pink root rot in various crops. In the present study, the density of S. terrestris was estimated by quantitative real-time PCR in onion and non-onion fields of Hokkaido, the northernmost island of Japan. Three-year observations in monoculture and rotation fields demonstrated that the fungus grew significantly from the third year onwards, and declined in fields planted with poor hosts (e.g., sugar beet and soybean) that produced few or no chlamydospores of S. terrestris. Seasonal analysis revealed that the population of S. terrestris consistently increased when the tops of onions fell over in summer, which is when root activity declines. However, the soil inoculum potential estimated by a seedling bioassay showed distinct seasonal patterns, which rose from post-harvest in winter and remained high until the subsequent planting in spring. Detailed surveys on depth distribution in an onion field detected a high population of S. terrestris in the effective layer (10–30 cm deep) but not below the hardpan (40 cm), implying that the fungus is intimately associated with roots. These results indicate that the proliferation of the fungus is closely related to root senescence and that over-wintered propagules play an important role in primary infections, affecting disease severity. The present study shows that the temporal dynamics of S. terrestris depend exclusively on the activity of infecting roots and provides circumstantial evidence on the deleterious impact of monoculture on crop production. 相似文献
162.
Ryo Nakagami Sotaro Chiba Naoto Yoshida Yoshiteru Senoo Minako Iketani-Saito Satoru Iketani Hideki Kondo Tetsuo Tamada 《Plant pathology》2022,71(3):715-728
Beet necrotic yellow vein virus (BNYVV) is the causal agent of rhizomania, the most serious sugar beet disease worldwide. Since the first finding in Japan in 1969, BNYVV became widespread throughout Hokkaido in a few decades and led to the introduction of Rz1-resistant sugar beet cultivars in the 1990s. Here, we report the historical progress of the BNYVV epidemic in Hokkaido from 1969 to 2019. Previous analysis on samples from 1991 showed that BNYVV isolates were classified into three strains (named O, D, and T) based on the RNA3-encoded p25 gene. The O-type viruses were widely detected in Hokkaido, while the D- and T-type viruses were detected in limited areas. The RNA5, encoding the p26 gene, was initially contained in some D- and O-type isolates but not in any T-type isolates. Interestingly, recent sample analysis revealed that RNA5-containing T-type viruses, seemingly more virulent than the other two strains, were widely detected in Hokkaido. Additionally, a small group of virus isolates harbouring a new p25 gene (named C) was found in limited areas. These results suggest that the T-type viruses, which accompanied RNA5, have been preferentially spread from a limited area to other districts over the last few decades and that this spread might be strongly associated with the recent introduction of Rz1-resistant sugar beet cultivars. BNYVV-positive samples also contained mainly beet soil-borne virus and traces of beet virus Q, both of which are the first to be recorded in Japan. 相似文献
163.
Tatsuki TAKAHASHI Maho INUKAI Fumiki IZUMI Yuji FUJII Shoko NISHIYAMA Tatsunori MASATANI Makoto SUGIYAMA Naoto ITO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(11):1508
The rabies virus strain Komatsugawa isolated from a dog in Tokyo in the 1940s retains biological properties as a field strain, providing an effective model for studying rabies pathogenesis. To facilitate molecular studies on the pathogenesis, this study aimed to establish a reverse genetics system for the Komatsugawa strain. By transfecting the full-length genome plasmid of this strain, infectious virus with artificially introduced genetic markers in its genome was rescued. The recombinant strain had biological properties similar to those of the original strain. These findings indicate that a reverse genetics system for the Komatsugawa strain has successfully been established. 相似文献
164.
Dai Kusumoto Hayato Masuya Kazuya Ohmura Naoto Kamata 《Journal of Forest Research》2012,17(4):393-396
This study was conducted to compare the virulences of various isolates of Raffaelea quercivora—a fungus that causes Japanese oak wilt disease—towards Japanese oak trees. Five isolates were collected from a wide range of Japan and inoculated into Quercus serrata logs and Q. crispula saplings. The tangential length of the discolored sapwood in the Q. serrata logs differed significantly among the isolates. The trend in isolate virulence was similar for the Q. serrata logs and the Q. crispula saplings. This is the first report suggesting that there is variability in virulence among isolates of R. quercivora. 相似文献
165.
Takahiro Nishimaki Takayuki Ibi Yoshihiro Tanabe Yoshiyuki Miyazaki Naohiko Kobayashi Tamako Matsuhashi Takayuki Akiyama Emi Yoshida Kazumi Imai Mayu Matsui Keiichi Uemura Naoto Watanabe Tatsuo Fujita Yosuke Saito Tomohiko Komatsu Takahisa Yamada Hideyuki Mannen Shinji Sasazaki Tetsuo Kunieda 《Animal Science Journal》2013,84(8):585-591
Japanese Black cattle are at risk for genetic homogeneity due to intensive use of a few sires. Therefore, assessment of the actual genetic diversity of this breed is important for future breeding plans. In the present study, we investigated the genetic diversity within and among eight subpopulations of Japanese Black cattle using 52 microsatellite markers. The parameters for genetic diversity of Japanese Black cattle were comparable to those of other cattle breeds, suggesting that the relatively high genetic diversity of the breed. However, upon comparison among the eight subpopulations, the Hyogo subpopulation showed markedly low genetic diversity. The results of the pairwise FST values, phylogenetic network and structure analysis indicated that the Hyogo population has remarkably high level of genetic differentiation from other populations, while Yamagata, Niigata, Hiroshima and Kagawa populations have low levels of genetic differentiation. Furthermore, multidimensional scaling plots indicated that individuals in some subpopulations were separated from individuals in the other subpopulations. We conclude that while the overall genetic diversity of Japanese Black cattle is still maintained at a relatively high level, that of a particular subpopulation is significantly reduced, and therefore the effective population size of the breed needs to be controlled by correct mating strategies. 相似文献
166.
Yukinori Shimada Naoto Murakami Tatsuo Tsuzaki Tadahisa Seikai 《Fisheries Science》2007,73(2):364-370
ABSTRACT: To improve stock enhancement strategies, the influences of repeated cross-breeding on several traits were studied in hatchery-reared juveniles of the Japanese flounder. Ten families (seven caught in the wild and three from cross-bred brood fish) were produced by artificial fertilization. Ninety-four days after hatching, we investigated settlement, burying in the sand and escape behavioral traits, and then measured tolerance of high salinity and six morphological traits. Juveniles from the repeatedly cross-bred brood fish showed significantly higher growth and tolerance than those from the wild-caught brood fish. Among the behavioral traits, a significantly lower settling speed and incidence of burying in the sand were observed in the juveniles from the repeatedly cross-bred families. Heritability ( h 2 ) of growth traits was more than 0.2 in both origins, while that of other traits was close to zero. These results suggest that repeated cross-breeding of Japanese flounder under hatchery conditions improves growth performance and leads to the loss of certain behavioral traits that are considered essential to survival in wild environments. 相似文献
167.
Nakamura K Mase M Yamamoto Y Takizawa K Kabeya M Wakuda T Matsuda M Chikuba T Yamamoto Y Ohyama T Takahashi K Sato N Akiyama N Honma H Imai K 《Avian diseases》2011,55(4):719-723
From January 2009 to June 2010, many broiler chicks suddenly died without clinical signs. The mortality rates were from 1.2% to 17.0% in affected flocks. Inclusion body hepatitis (IBH) was detected in 13 prefectures (northern, eastern, western, and southern areas) in Japan. The livers were enlarged and pale. The bursa of Fabricius and thymus had not atrophied. Multifocal necroses of hepatocytes with basophilic intranuclear inclusions were seen in the liver. Eosinophilic intranuclear inclusion bodies in hepatocytes were rare. Focal necrosis of acinar cells with basophilic intranuclear inclusions was found in the pancreas. Basophilic intranuclear inclusion bodies were detected in intact surface epithelial cells of gizzard and epithelial cells of the small intestine. The intranuclear inclusions of liver, pancreas, gizzard, and small intestine were stained positively for immunohistochemistry of fowl adenovirus (FAV) antigen. Ultrastructurally, basophilic intranuclear inclusions consisted of viral particles approximately 70 nm in diameter and arranged in a crystalline array. FAV was isolated from the liver of chickens affected with IBH. The serotype of most isolates was 2. This study suggests that IBH produced by FAV is epidemic in broiler chicks in Japan and that the present cases occurred as the primary disease without the association of infectious bursal disease virus or chicken anemia virus. 相似文献
168.
Naoto Nihei Shigeto Fujimura Keitaro Tanoi Nobuo Yamashita Sho Morimoto Tomoko M. Nakanishi 《Soil Science and Plant Nutrition》2016,62(2):117-120
Radioactive substances were released into the environment after the nuclear accident at the Fukushima Daiichi Nuclear Power Station; this led to the contamination of the soil at Fukushima Prefecture. Mixing of organic matter with soil during plowing is known to influence radiocesium (134Cs and 137Cs) absorption by crops. However, the effect of mixing organic matter polluted by radioactive substances during plowing on radiocesium absorption by plants is not yet known. The aim of this study was to investigate the effect on the radiocesium absorption by komatsuna (Brassica rapa L. var. perviridis) cultivated in a 45-L container containing Andosol (14,300 Bq kg?1) or Gray Lowland soil (33,500 Bq kg?1) mixed with polluted wheat (Triticum aestivum L. Thell.) straw (2080 Bq kg?1). The radiocesium concentration of the plants and the soil and the amount of exchangeable radiocesium in the soil were determined using a germanium semiconductor. The transfer of radiocesium from the soil to plants decreased by 53 and 27% in Andosol and Gray Lowland soil, respectively, after the application of 10 t ha?1 polluted wheat straw. This reduction in the level of radiocesium transfer might be attributed to potassium contained in the wheat straw, which might compete with cesium during membrane transport and thereby block the transport of cesium from the soil solution to the roots and from the roots to the shoots. Alternatively, the applied wheat straw probably absorbed radiocesium and decreased the amount of exchangeable radiocesium in the soil. Our findings suggest that the mixing of polluted wheat straw with contaminated soil might influence the absorption of radiocesium content by agricultural products. Further studies are warranted to determine the long-term effects of the application of polluted wheat straw on the rate of radiocesium transfer to crops. 相似文献
169.