Host and virus effects on reversion in cassava affected by cassava brown streak disease

The phenomenon of virus‐infected plants naturally recovering health is known as ‘reversion’, and is a type of resistance mechanism exploited in some crop plants for disease control. Various parameters were investigated that affect reversion from cassava brown streak disease (CBSD) in three cassava varieties (Albert, Kaleso and Kiroba) that differ in levels of resistance to the disease. Cassava plants were inoculated by grafting with two virus species (Ugandan cassava brown streak virus, UCBSV and Cassava brown streak virus, CBSV) that cause CBSD, and the plants grown from them were subsequently assessed for reversion. The rate of reversion depended on the cassava variety, virus species, and the length and position of the stem cuttings used. A significantly high proportion of progenies were virus‐free (reverted) for the resistant variety Kaleso (64·1% for UCBSV and 54·9% of CBSV), compared to the tolerant variety Kiroba (56·7 and 45·5%) and the susceptible control Albert (38·9 and 35·1%). The highest number of virus‐free plants was generated from short 10 cm long cuttings (e.g. 60·1% for Kaleso for CBSV) compared to 20 cm long stem cuttings (e.g. 21·4% for Albert). Cuttings taken from upper stems of diseased plants produced most virus‐free progenies compared to middle and lower parts. More than 50% virus‐free plants were obtained in the resistant and tolerant varieties. This is a highly valuable finding and could be exploited for developing strategies to control the current CBSD epidemic in eastern and central Africa.     

Selenium and copper status of camels in Al-Jouf area (Saudi Arabia)

In order to understand the changes in copper and selenium status in camel dam and calf around the calving period, blood samples were collected in 26 she-camel before delivery and after as well as their calves after birth. The mean values for the mother and their newborn were respectively 70.3 ± 19.8 and 58.6 ± 13.9 μg/100 ml for copper, 5.3 ± 3.7 and 4.6 ± 1.7 μg/100 ml for selenium. No change was observed for copper, but selenium increased after parturition in 81% of the case. The selenium status of camel calf was correlated with those of its mother, but not the copper. As the whole the correlation between selenium and copper was significantly positive. The selenium status was improved in camel receiving diet enriched with barley. The maternal transfer to milk has to be investigated.     

Influence of processing cassava peels on the hydrogen cyanide concentration, nutritive value and performance of growing rabbits

Forty-eight rabbits used to investigate the response of growing rabbits to diets containing differently processed cassava peel meals were divided into four groups balanced for sex and weight. These groups were then assigned at random to the experimental treatments. Four diets were formulated such that the diets contained 200 g/kg of the experimental feedstuffs, sun-dried cassava peel meal (S), ensiled cassava peel meal (E) and retted cassava peel meal (R), and the control did not contain any cassava peels. Hydrogen cyanide (HCN) concentration in the processed cassava peel meal was lower relative to the unprocessed meal. It was, however, lowest in R and highest in E. With the exception of S, fibre fractions were lower in the processed meal compared to the unprocessed meal. The E depressed (p < 0.05) feed and digestible nutrient intakes, weight gain, nutrient digestibility, digestible protein (DP), digestible energy (DE) and DP/DE ratio but increased protein intake to gain and feed to gain ratios compared to other diets. Intake of HCN decreased (p < 0.05) successively in this order: R, S and E. The results indicate that retting and sun-drying are more effective in cassava peel detoxification than ensiling, and dietary HCN concentration and intake of 56 and 4 mg/kg BW, respectively, were not toxic under the conditions of the experiment.     

Management of fungal root‐rot pathogens in tropical Acacia mangium plantations

Acacia mangium is a fast‐growing tree species. It is mainly planted in large monocultures for pulpwood in South‐East Asia. Root rot has become the most economically damaging disease of this species with high tree mortality rates observed during second and third rotations. Two main types of root rots have been found in A. mangium, viz. brown root‐rot and red‐root disease caused by Phellinus spp. and Ganoderma spp., respectively. To assess the future management options for root rot of A. mangium, we review past and current disease‐management strategies for root rot in different temperate and tropical industrial tree crops. The efficacies of a wide range of silvicultural, chemical and biological options are detailed, and their potential utilization in managing root rot of A. mangium is discussed. We conclude that the current gaps in knowledge regarding identification, biology and disease epidemiology of the root‐rot pathogens will need to be addressed so that effective management options can be developed.     

A winter intercrop of faba bean and rapeseed for silage as a substitute for Italian ryegrass in rotation with maize

In order to combine the ability of legumes to fix atmospheric nitrogen and the cruciferous capacities to mobilize soil nutrients and herbicide action, the aim of this work was to evaluate an alternative winter intercrop (faba bean-rapeseed) as a replacement of Italian ryegrass culture in a rotational system with maize as summer crop. For this purpose, two adjacent plots were used during three agronomic years (2011–2012, 2012–2013 and 2013–2014) to evaluate the agronomic performance through the forage production, nutritional composition of forage and silage, and the effects on soil fertility. The Italian ryegrass was cultivated under conventional management: using chemical fertilization and recommended dosages of herbicides. The faba bean-rapeseed intercrop was cultivated under an alternative management: organic fertilization and less herbicide supply. The intercrop provides higher forage yield per hectare than Italian ryegrass, with greater protein (kg ha^?1) and similar energy (GJ ha^?1) yields. The intercrop allows reducing the inputs of chemical fertilization and herbicides, and it has a positive effect on the balance of soil nutrients, especially increasing the potassium, calcium and magnesium contents. The results show that faba bean-rapeseed intercrop could be an alternative to the Italian ryegrass as winter crop.     

Effect of protection against hot climate on growth performance,physiological response and endocrine profile of growing lambs under semi-arid tropical environment

In the hot semi-arid tropical region, extreme summer is a major constraint in sheep production. The growth performance of growing lambs is impaired during the summer. Therefore, the present study was conducted to assess the effect of protection against hot climate on growth performance, physiological response, and endocrine profile of growing lambs under semi-arid tropical environment. All the data in the experiment were presented as mean ± SE. Thirty Malpura lambs with the age of 122.7 ± 6.05 days and body weight of 19.52 ± 0.42 kg were allotted into three groups, viz. G1 (control, lambs kept in open area under tree shade), G2 (lambs kept in conventional asbestos-roofed shed), and G3 (lambs kept in designed shed). The allotment was based on their initial body weight so that the mean body weight of each group was similar. In all three types of protection, lambs were provided with sufficient space to move and social interaction. In asbestos-roofed shed (G2), all the four sides from ground to the roof were fenced with strong galvanized iron chain link. But in G3, all the four sides were constructed in a manner that there was 0.13 m air space between inner and outer solid brick columns of the wall up to 1.37 m height, and rest up to the roof was fenced with bamboo splint net. The air space between the two columns of the wall was filled with sand, and it was kept in moist condition by continuous water drips which provide extra evaporative cooling. The designed shed was basically constructed with bamboo. Tree shade was assured under the natural shades of large trees. The shaded area was protected by wire fences. The experiment was conducted for 45 days during very high temperature (May–June). The lambs were provided with ad libitum green fodder, dry roughage, 300 g concentrate, and ad libitum drinking water. The respiration rate at morning and afternoon, pulse rate at morning and afternoon and rectal temperature at afternoon was significantly (P < 0.05) lower in lambs of G3 as compared to other groups. The final body weight did not differ significantly between the groups, but average daily gain (g/day) was higher in G3 (150.06 ± 11.13) as compared to the G1 (109.69 ± 18.10). The feed conversion rate (FCR) was significantly (P < 0.05) better in G3 (5.49 ± 0.08) as compared to G1 (7.65 ± 0.08) and G2 (6.60 ± 0.08). The hemoglobin level was significantly (P < 0.05) higher in G1. The endocrine profile did not vary significantly (P > 0.05) among the groups, but insulin-like growth factor-1 (IGF-1) was higher in G3. It can be concluded from this study the lambs provided with better protection during summer remained in more comfortable condition, showed better FCR, and although not statistically significant showed better growth performance.

     

<Emphasis Type="Italic">Mycobacterium bovis</Emphasis>, but also <Emphasis Type="Italic">M. africanum</Emphasis> present in raw milk of pastoral cattle in north-central Nigeria

Using deletion typing technique, five mycobacteria isolated from unpasteurised milk samples from cows in north-central Nigeria were characterized as Mycobacterium bovis (n = 4) and M. africanum (n = 1). This report emphasizes that transmission between the animal and human reservoir is a serious threat in Nigeria.     

Effect of nitrogen on yield,quality, and irrigation water use efficiency of drip fertigated grafted watermelon (Citrullus lanatus) grown on a calcareous soil

A two-year field experiment was conducted during the growing seasons 2016 and 2017 to investigate the response of drip fertigated watermelon to different rates of nitrogen (0, 10, 20, 30, and 40?g/m³ N) applied in the form of ammonium sulfate (AS). The combined effect of nitrogen (N) and sulfur (S) was investigated. Irrigation water use efficiency (IWUE) under the different N levels was, also, evaluated. N levels in the irrigation water induced a significant effect on yield parameters (total and commercial yields, dry matter yield, plant yield, and fruit number). Increasing the level of N also induced significant increases in fruit length and fruit fresh weight. IWUE was also significantly improved at high levels of N. Fertigation using modern techniques can be considered an efficient method in the delivery of N to the crop at N levels of 30–40?g/m³, as ammonium sulfate.     

Protective Role of a methanolic Extract of Spinach (<Emphasis Type="Italic">Spinacia oleracea</Emphasis> L.) Against Adverse Effects of UV-C Irradiation on Fenugreek (<Emphasis Type="Italic">Trigonella foenum-graecum</Emphasis> L.) Seedlings

Photosynthesis is an important process for plants in which plants utilize the energy from sunlight to manufacture and produce all their own food. Although UV-C damage is not physiologically relevant for plants growing in the sun, short-wavelength (UV-C) radiation from germicidal lamps has been used to study cell damage in animals as well as in plants. This study provides a review of the effects of a methanolic extract of spinach (SE) as a natural powerful antioxidants used at two concentrations (25 and 50?ppm) on fenugreek seedlings grown under UV-C stress conditions. The results revealed that exposure of fenugreek seedlings to low UV-C period (30?min) increased growth criteria of the produced seedlings. In addition, the contents of chlorophyll, total pigments, total protein, free amino acids, non-enzymatic (carotenoids, total phenol, flavonoids, α?tocopherol & ascorbic acid contents) and enzymatic antioxidants were increased at this period. By increasing exposure periods of UV-C radiation, the growth criteria and chemical constitutes of the seedlings were decreased. Application of lower concentration of SE (25?ppm) improve the growth of the seedlings at 30-min UV-C and was able to protect seedlings from high periods of UV-C irradiation by increasing all measured parameters except CAT. These increases might be an adaptive mechanism to minimize the adverse effects of longer exposure of UV-C radiation. Moreover, treating the irradiated seedlings with spinach extract led to an obvious alteration in gene expression including synthesis of some proteins bands and disappearance of other protein sets of the protein profile.     

Seroprevalence and risk factors for bovine brucellosis in Jordan

We investigated the seroprevalence and risk factors for Brucella seropositivity in cattle in Jordan. The sera from 671 cows were randomly collected from 62 herds. The antibodies against Brucella were detected using a Rose Bengal plate test and indirect ELISA. A structured questionnaire was used to collect information on the cattle herds'' health and management. A multiple logistic regression model was constructed to identify the risk factors for Brucella seropositivity. The true prevalence of antibodies against Brucella in individual cows and cattle herds was 6.5% and 23%, respectively. The seroprevalence of brucellosis in cows older than 4 years of age was significantly higher than that in the younger cows. The seroprevalence of brucellosis in cows located in the Mafraq, Zarqa and Ma''an governorates was significantly higher than that of the other studied governorates. The multiple logistic regression model revealed that a larger herd size (odd ratio = 1.3; 95% CI: 1.1, 2.6) and mixed farming (OR = 2.0; 95% CI: 1.7, 3.7) were risk factors for cattle seropositivity to Brucella antigens. On the other hand, the use of disinfectants (OR = 1.9; 95% CI: 1.1, 2.1) and the presence of adequate veterinary services (OR = 1.6; 95% CI: 1.2, 3.2) were identified as protective factors.