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31.
LI Yao WEI Xin-yi YAN Jun-li WANG Mo WU Dao-qi ZHANG Gao-fu YANG Hai-ping LI Qiu 《园艺学报》2017,33(2):308-314
AIM: To investigate the aggravating effect of albumin overload on the kidney injury induced by lipid nephrotoxicity, and to observe the renoprotective effect of simvastatin (SIMV) on adriamycin nephropathy (ADR) mice.METHODS: SPF healthy male BALB/c mice were randomly divided into control group, ADR group, ADR with bovine serum albumin (BSA) overload (ADR+BSA) group, and ADR with both BSA overload and SIMV treatment (ADR+BSA+SIMV) group. All mice were uninephrectomized under general anesthesia 2 weeks before setting up ADR model. ADR+BSA model started to be set up 4 weeks later. At the end of the 0th, 2nd, 6th, 10th and 14th weeks, 24 h urinary protein was evaluated. At the end of the 14th week, the serum biochemical indexes and the kidney pathological changes were observed, and glomerulosclerotic index (GSI) was also evaluated. The cholesterol in the kidney was measured by enzymic colorimetric method and oil red O staining. The expression of IL-1β, TGF-β1 and low-density lipoprotein receptor (LDLr) in the kidney tissues was determined by real-time PCR. The expression of IL-1β and IL-17 was measured by immunohistochemistry and the expression of IL-17 in the kidney was measured by ELISA.RESULTS: Compared with control group, the expression of IL-1β, TGF-β1, IL-17 and LDLr, and cholesterol content in the kidney and the GSI were all significantly increased in ADR group (P<0.05). Compared with ADR group, 24 h urinary protein, serum creatinine, the expression of IL-1β, IL-17 and LDLr, and cholesterol content in the kidney were all significantly increased in ADR+BSA group (P<0.05). Treatment with SIMV significantly decreased the expression of IL-1β, TGF-β1, IL-17 and LDLr. The accumulation of cholesterol in the kidney and the GSI were also decreased (all P<0.05).CONCLUSION: Inflammation aggravates the lipid deposition and glomerular sclerosis by increasing the expression of LDLr in ADR mice. Albumin overload further accelerates the progressive kidney damage by regulating the expression of IL-1β, TGF-β1 and IL-17, which promotes the increase in LDLr. The beneficial effect of SIMV might be mediated by its anti-inflammatory effect. 相似文献
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在生物芯片上通过测量微(纳)电极阻抗值以诊断溶液中禽流感病毒,是目前禽流感病毒快速检测的方向之一.以AD5933高精度阻抗转换芯片为核心测量芯片,以ATmega16单片机为控制器,通过外围电路设计和调试,研制激励频率范围1~100 kHz、测量阻抗范围1~1 000 kΩ的阻抗测试仪,通过液晶实时显示扫频和定点测量结果.为保证测试仪的准确性,设计了带有35组不同阻抗组合的校正网络,用以实时校正.经精度为0.2%的商品化阻抗测试仪标定,所研制的原理性装置容抗测量精度达3%,通过进一步改进后可以满足使用要求. 相似文献
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根据甘蔗收获机切割器动态分析结果中应力集中区域较多、振动较大的问题,针对减小甘蔗破头率和能耗及提高整体刚度的需求,在满足工作条件的情况下,以最小柔度为优化目标,对切割器结构进行拓扑优化设计,以优化所得构型为基础,提取并建立切割器新模型。最后,对新模型进行有限元分析,结果表明:优化后的切割器的1阶固有频率提高了10.05%,2阶固有频率提高了15.15%,Z向位移和综合位移分别减少了55.58%和44.58%,质量也减少了3.37%,应力集中区域刚性得到明显提升,为同类甘蔗收获机切割器的优化设计提供了参考和理论依据。 相似文献
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用6种不同的贮藏方法进行果蔗常温保鲜试验,结果发现垫盖塑料薄膜的平行堆蔗处理能使贮藏环境维持较高的相对湿度,降低日最高温度,有利于防病保鲜,贮藏70天后仅干耗13.60%,此对照低208.82%,好蔗率达92.0%(对照全部坏变),且尚能保持一定的蔗汁锤度,清甜可口。 相似文献
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在3个甘蔗品种的分蘖初期,分别喷施硝酸镧和混合稀土溶液,结果这两种处理都能促使甘蔗叶片在不同生长期的多酚氧化酶活性极显著高于喷清水的对照,而且喷施混合稀土的又极显著高于硝酸镧处理。混合稀土还能使即将进入工艺成熟期的甘蔗叶片的过氧化物酶活性高于对照。 相似文献
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M. Sajjad Mirza Samina Mehnaz Philippe Normand Claire Prigent-Combaret Yvan Moënne-Loccoz René Bally Kauser A. Malik 《Biology and Fertility of Soils》2006,43(2):163-170
Nitrogen-fixing plant growth-promoting rhizobacteria (PGPR) from the genus Pseudomonas have received little attention so far. In the present study, a nitrogen-fixing phytohormone-producing bacterial isolate from kallar grass (strain K1) was identified as Pseudomonas sp. by rrs (16S ribosomal RNA gene) sequence analysis. rrs identity level was high with an uncharacterized marine bacterium (99%), Pseudomonas sp. PCP2 (98%), uncultured bacteria (98%), and Pseudomonas alcaligenes (97%). Partial nifH gene amplified from strain K1 showed 93% and 91% sequence similarities to those of Azotobacter chroococcum and Pseudomonas stutzeri, respectively. The effect of Pseudomonas strain K1 on rice varieties Super Basmati and Basmati 385 was compared with those of three non-Pseudomonas nitrogen-fixing PGPR (Azospirillum brasilense strain Wb3, Azospirillum lipoferum strain N4 and Zoogloea strain Ky1) used as single-strain inoculants. Pseudomonas sp. K1 was detected in the rhizosphere of inoculated plants by enrichment culture in nitrogen-free growth medium, which was followed by observation under the microscope as well as by PCR using a rrs-specific primer. For both rice varieties, an increase in shoot biomass and/or grain yield over that of noninoculated control plants was recorded in each inoculated treatment. The effect of Pseudomonas strain K1 on grain yield was comparable to those of A. brasilense Wb3 and Zoogloea sp. Ky1 for both rice varieties. These results show that nitrogen-fixing pseudomonads deserve attention as potential PGPR inoculants for rice. 相似文献