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21.
Seko Takuya Imamura Shintaro Ishihara Kenji Yamashita Yumiko Yamashita Michiaki 《Fisheries Science》2019,85(4):731-736
Fisheries Science - Selenoneine is a selenium-containing compound that exhibits strong radical-scavenging activity. Here we present a novel function of selenium in which selenoneine exhibits... 相似文献
22.
Fisheries Science - The quality of fish muscles is known to vary with the season. The characterisation of such seasonal variations is important for the development of fish products of high... 相似文献
23.
Shiro Itoi Koki Ikeguchi Masaki Kaneniwa Ryuji Kuwahara Ichiro Ohara Noriko Ishida Michiaki Yamashita Shugo Watabe 《Fisheries Science》2007,73(2):429-439
ABSTRACT: Fast skeletal muscles of Japanese flounder Paralichthys olivaceus and red sea bream Pagrus major were examined for quantitative and qualitative changes of mitochondrial ATP synthase (Fo F1 -ATPase) in association with rearing temperatures. The specific activities of Fo F1 -ATPase from Japanese flounder reared at 10°C, 15°C and 25°C for 4 weeks were determined to be 81 ± 11, 74 ± 13 and 83 ± 11 nmol/min·mg mitochondrial protein, respectively. The corresponding activity from red sea bream reared at 8°C for 5 weeks was determined to be 65 ± 9 nmol/min·mg mitochondrial protein, which was higher than 33 ± 9 nmol/min·mg mitochondrial protein in fish reared at 23°C. The contents of α- and β-F1 -ATPase in total mitochondrial proteins were not significantly different between fish reared at different temperatures for the two fish species. However, the contents of β-F1 -ATPase in the total fast skeletal muscle extracts, prepared from Japanese flounder reared at 10°C, were 2.1- and 2.9-fold higher than those for fish reared at 15°C and 25°C, respectively. The corresponding content from red seabream reared at 8°C was 2.2-fold higher than that for fish reared at 23°C. Therefore, the changes in Fo F1 -ATPase depending on rearing temperatures were species-specific. 相似文献
24.
To characterize post-mortem changes in fish meat quality during chilled and frozen storage, muscle toughness and the connective tissue structure of muscle were compared between two mackerel species, spotted mackerel Scomber australasicus and Pacific mackerel S. japonicus. Measurement of the breaking strength of meat slices from both non-frozen fresh fish and frozen-thawed fish revealed that spotted mackerel meat was softer than Pacific mackerel meat. Under scanning electron microscopic observation, the connective tissue of non-frozen meat of spotted mackerel was thinner than that of Pacific mackerel. In addition, the collagen content in the spotted mackerel meat was lower than that of Pacific mackerel. These findings suggest that connective tissue structure might influence muscle toughness in mackerel. 相似文献
25.
Sasaki N Yamada M Morita Y Furuoka H Itoh M Satoh M Yamada H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(12):1367-1369
We report a case of EMND in a heavy horse that was bred and trained in Hokkaido, Japan. Clinical symptoms included severe ataxia of all four limbs, tilted head, lethargy, and flaccid lips. Numerous axonal degenerations and swellings were observed in nuclei, mostly in the cerebellar dentate nucleus and the nucleus of the hypoglossal nerve, and in the ventral horn of the spinal cord. In the ventral horn of the spinal cord, neuronal degeneration, swelling, and/or necrosis were observed sporadically. The case was diagnosed as EMND from the clinical symptoms and pathological findings. 相似文献
26.
Possible implication of metalloproteinases in post-mortem tenderization of fish muscle 总被引:1,自引:1,他引:1
Mitsutoshi Kubota Masato Kinoshita Satoshi Kubota Michiaki Yamashita Haruhiko Toyohara Morihiko Sakaguchi 《Fisheries Science》2001,67(5):965-968
ABSTRACT: It is suspected that the proteolytic breakdown of extracellular matrix proteins is responsible for the postmortem tenderization of fish muscle during chilled storage. In order to identify the type(s) of proteinases involved in this phenomenon, the effect of proteinase inhibitors, EDTA (ethylenediamine tetraacetic acid), 1,10-phenanthroline, ρ-APMSF [(ρ-amidinophenyl) methanesulfonyl fluoride hydrochloride] and E-64 [ L - trans -epoxysuccinyl-leucylamido-(4-guanidinobutane)] on tenderization was investigated by using Japanese flounder. Proteinase inhibitor solution was injected into a blood vessel in a caudal portion of live flounder and the firmness of muscle was then evaluated as a shear force value at 0 h and 6 h after death. Metalloproteinase inhibitors, EDTA and 1,10-phenanthroline, significantly suppressed postmortem tenderization. These findings suggest that metalloproteinases are candidates for proteinases involved in the postmortem tenderization of fish muscles. Although not significantly, p -APMSF, a serine proteinase inhibitor, partially suppressed muscle tenderization, which suggests that serine proteinases are also implicated in postmortem tenderization. A cysteine proteinase inhibitor, E-64, showed no effect, suggesting that cysteine proteinases are not involved. 相似文献
27.
Namikoshi Atsushi Takashima Yasuharu Iguchi Jun Yanagimoto Takashi Yamashita Michiaki 《Fisheries Science》2011,77(4):671-678
Fish species identification techniques for authentic food labeling were developed using species-specific PCR primers for cod
roe products. A salted, seasoned fish roe product, karashimentaiko (chilli cod roe), is produced from the eggs of Alaska pollock, Theragra chalcogramma, according to the fair trade competition agreement authorized by the Fair Trade Commission of the Japanese government. To
examine whether Alaska pollock ovaries or those of other fish species are being used as raw materials for the fish roe products,
we developed species identification techniques using PCR amplification of a 255-bp fragment encoding the mitochondrial ATP
synthase Fo subunit 6 (ATP6) gene with a species-specific primer set for Alaska pollock mitochondrial DNA. We also designed two species-specific primer
sets corresponding to the mitochondrial ATP6 and cytochrome b (cytb) for Gadus spp. and Micromesistius spp. by PCR amplification of 332- and 223-bp fragments, respectively. We examined the species specificity of these PCR-based
methods among nine commercially important Gadidae species. 相似文献
28.
Isaka M Imamura M Sakuma I Makino Y Shiiya N Yasuda K 《Research in veterinary science》2007,82(1):110-114
Calcitonin gene-related peptides (CGRP), which are potent vasodilators, are elevated during cardiopulmonary bypass (CPB) in humans. We evaluated the plasma levels of CGRP in dogs during CPB with hemofiltration with and without hemodilution. Female beagles were divided into control (n=5) and hemodilution (n=5) groups. The CPB with hemofiltration was performed with or without hemodilution. For the measurement of CGRP, blood samples were collected pre-CPB, during CPB, and post-CPB. The concentrations of CGRP in the hemofiltration solution were measured. Although the CPB elevated the plasma CGRP levels in both groups, its elevation was significant in the hemodilution group when compared to the pre-CPB levels. CGRP levels returned to normal post-CPB. Significant differences were found between the two groups in the CGRP amount in hemofiltration. The results show that hemofiltration should be used during CPB to decrease the plasma levels of CGRP. 相似文献
29.
Michiaki Iwata Kenji Umemura Takeshi Teraoka Hideki Usami Yukiko Fujisawa Yukimoto Iwasaki 《Journal of General Plant Pathology》2003,69(1):83-86
Certain cellular responses to stresses and stimuli are regulated by a G-protein-mediated signaling pathway. A rice dwarf
mutant that is defective in the α subunit of the heterotrimeric G-protein was found to be fully protected from blast fungus
by the plant activator probenazole (PBZ) despite the 1-day delay in induction of the PR-10 gene PBZ1 by PBZ. These results suggest that the signaling pathway for protection by PBZ is not via the G-protein, although G-protein
is involved in the induction of PBZ1 by PBZ.
Received: March 27, 2002 / Accepted: August 20, 2002 相似文献
30.
Glutathione peroxidase GPx1 was purified from the red muscle of Pacific bluefin tuna Thunnus orientalis and its enzymatic properties were characterized. The enzyme was optimally active at pH 7.4 with a specific activity for hydrogen
peroxide and a K
m of 6.7 μM. cDNA was also isolated and it contained a predicted open reading frame (ORF) encoding a 188 amino acid protein.
The phylogenetic tree shows that fish including Pacific bluefin tuna, pufferfish, and zebrafish, but not mammals, possess
two genetically distinct types of GPx1, i.e., GPx1a and GPx1b. The purified enzyme was classified as a fish GPx-1b enzyme
on the basis of the phylogenetic tree of the GPx1 family. 相似文献