全文获取类型
收费全文 | 20885篇 |
免费 | 995篇 |
国内免费 | 1483篇 |
专业分类
林业 | 2199篇 |
农学 | 2235篇 |
基础科学 | 1169篇 |
3589篇 | |
综合类 | 5284篇 |
农作物 | 1080篇 |
水产渔业 | 1026篇 |
畜牧兽医 | 4523篇 |
园艺 | 773篇 |
植物保护 | 1485篇 |
出版年
2024年 | 39篇 |
2023年 | 203篇 |
2022年 | 456篇 |
2021年 | 748篇 |
2020年 | 650篇 |
2019年 | 679篇 |
2018年 | 501篇 |
2017年 | 586篇 |
2016年 | 651篇 |
2015年 | 811篇 |
2014年 | 750篇 |
2013年 | 980篇 |
2012年 | 1340篇 |
2011年 | 1455篇 |
2010年 | 1143篇 |
2009年 | 1053篇 |
2008年 | 1254篇 |
2007年 | 1323篇 |
2006年 | 1210篇 |
2005年 | 1100篇 |
2004年 | 749篇 |
2003年 | 685篇 |
2002年 | 637篇 |
2001年 | 322篇 |
2000年 | 344篇 |
1999年 | 432篇 |
1998年 | 399篇 |
1997年 | 352篇 |
1996年 | 325篇 |
1995年 | 294篇 |
1994年 | 266篇 |
1993年 | 213篇 |
1992年 | 214篇 |
1991年 | 222篇 |
1990年 | 163篇 |
1989年 | 141篇 |
1988年 | 125篇 |
1987年 | 66篇 |
1986年 | 55篇 |
1985年 | 43篇 |
1984年 | 42篇 |
1983年 | 38篇 |
1982年 | 27篇 |
1981年 | 21篇 |
1980年 | 31篇 |
1979年 | 23篇 |
1978年 | 14篇 |
1977年 | 15篇 |
1976年 | 12篇 |
1973年 | 17篇 |
排序方式: 共有10000条查询结果,搜索用时 46 毫秒
51.
利用硅胶柱和葡聚糖凝胶柱层析,从沙棘叶子中分离并鉴定出黄酮类成分,进一步分离得到4,4′-二羟基-2′-甲氧基查耳酮,并对其结构进行了结构鉴定。 相似文献
52.
53.
William F. Krise Michael A. Hendrix Wayne A. Bonney Susan E. Baker-Gordon 《Journal of the World Aquaculture Society》1995,26(4):384-389
The use of saline solutions was tested to determine their efficacy as replacements for ovarian fluid as sperm activators and to eliminate variability encountered with the use of Ovarian fluid. We tested fertilization rate of semen from eight males on Atlantic salmon Salmo salar eggs after five sperm-activating solutions and a non-activating saline were substituted for ovarian fluid. We used solutions shown acceptable for use with other salmonid species. The six solutions tested were a non sperm-activating phosphate-buffered saline (PBS, 7.2 g/L NaCl, 1.48 g/L Na2 HPO4 , 0.43 g/L K H2 PO4 ), a Tris buffer (6.99 g/L NaCl, 3.63 g/L Tris and 2.42 g/L glycine), a Borax buffer (12.2 g boric acid/L in solution 1, 76 g disodium tetraborate/L combined 100:118, then 1 L combined with 3.7 L water and 18 g NaCl), and three solutions of 9.25 g/L (125 mM) NaCl buffered to pH 6.0, 7.5, and 8.9. The latter five solutions activated sperm immediately on contact, while PBS required additional water to activate sperm. The PBS solution was the least effective (mean percent eyed eggs 37.6%) for egg fertilization. The mean percent eyed eggs for the other five saline solutions (range 78% to 86%) were not significantly different. Sperm from one male provided significantly lower egg fertilization (39.6%) when compared with the other seven males (67.2–87.4% egg fertilization). Percent egg fertilization was not related to number of live sperm cells per egg. Our results show that osmotically-balanced sperm-activation solutions, even those with a pH range from 6.0 to 8.9 provide adequate media for fertilization of Atlantic salmon eggs. Fertilization in a deactivation saline and water reactivation of sperm resulted in low egg fertilization. 相似文献
54.
本文应用聚合酶链反应(PCR)技术从构建的新城疫病毒(NDV)cDNA文库中扩增含编码F糖蛋白前体──Fo酶切位点序列的359bp的F蛋白基因cDNA片段。将此359bpcDNA片段经光敏生物素标记后,即成NDV-cDNA探针。该探针能特异性地从感染的尿囊液中检测出NDV强毒株和疫苗毒株的基因组RNA,而不与IBDv-dsRNA、AIBv-ssRNA、EDS76-dsDNA、MDV-dsDNA,FPV-dsRNA及AILV-dsDNA发生交叉杂交反应。试验结果表明:尽管该探钎含有编码Fo蛋白酶切位点序列的碱基顺序,但它还是不能把NDV的强、弱毒株区分开。这说明NDV强、弱毒株比区域内的碱基存在着相当大的同源性。不过,此探针对NDV来说具有特异性,这就为NDV的诊断技术开创了基因水平检测的新途径。 相似文献
55.
根据长江流域夏秋季的气候特点,采用杂交、回交、系统分离、活蛹缫丝和多代高温多湿环境定向培育等育种方法,分别育成了中系蚕品种“浒花”和日系蚕品种“秋星”。两者都具有抗性强、丝量高、茧丝质优以及繁育容易等特点。其一代杂交种经多次实验室鉴定、农村生产鉴定、试养以及全国蚕品种审定结果表明:该新品种体质强健、孵化、眠起、老熟齐一,茧丝量高,茧层率22%;鲜毛茧出丝率15—16%,茧丝品质优良、解舒率74%,净度93分,茧丝纤度2.53/D,茧丝长超1000m。 相似文献
56.
The known information on hymenopterous parasites of Nitidulidae was consolidated in this article. Species of parasites, nitidulid hosts and, where possible, plant host of the nitidulids are provided along with citations to sources of information. Thirty-three species of hymenopterous parasites are recorded in nine different families. Since nomenclature of several of the parasites has changed, it was updated with the assistance of specialists. 相似文献
57.
M. Angeles Achon Vicente Medina Michael Shanks Peter Markham George P. Lomonossoff 《European journal of plant pathology / European Foundation for Plant Pathology》1994,100(2):157-165
In order to characterise and classify an unknown maize-infecting potyvirus isolated from fields in northeast Spain, the entire coat protein gene and the C-terminal twothirds of the large nuclear inclusion protein (NIb) gene were cloned and sequenced. Protein sequencing enabled the cleavage site between the two proteins to be deduced and also revealed that on storage the viral coat protein undergoes a specific degradation in which the N-terminal 39 amino acids are removed. Comparison of the nucleotide sequence of the 3 non-coding region of the viral RNA and the predicted amino acid sequence of the coat protein with the equivalent regions of other members of the potyvirus group revealed that the Spanish virus is closely related to maize dwarf mosaic virus strain A. 相似文献
58.
Nix BE Leib MS Zajac A Zarakas K 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》1993,22(1):10-16
Nine combinations of dosages and concentrations of D-xylose were given orally to eight clinically normal, immature dogs. The concentrations and dosages of D-xylose consisted of 5%, 10%, and 20% at 250 mg/kg, 500 mg/kg, and 750 mg/kg. Serum samples were collected at 0, 30, 60, 90, 120, and 180 minutes. Serum xylose was quantitated using the phloroglucinol microassay technique. A peak in serum xylose concentration was seen for each treatment combination at 60 or 90 minutes after dosing. The dosage effect was important in influencing serum xylose values (P < 0.0001). As the test solution dosages increased from 250 mg/kg to 500 mg/kg and 750 mg/kg, serum xylose values (when dosage was analyzed over the length of the entire test) rose linearly (R(2) = 0.98). The treatment combinations of 5% and 20% xylose solutions dosed at 750 mg/kg produced the highest serum xylose values at the 60- and 90-minute peak intervals. The independent effect of concentration was significant (p < 0.001) but was overridden by the stronger dosage effect. Serum xylose concentrations varied little statistically (p > 0.05) when the 5%, 10%, and 20% solutions were compared at a specific dosage. 相似文献
59.
采用功率密度408.39 mW,能量密度244.60 J/cm~2的He-Ne激光照射交巢穴,治疗新生羔羊腹泻病,具有显效快、疗程短、方法简便、减少劳动强度及降低治疗费用等特点,是一种新的理疗方法。 相似文献
60.
家蚕蛹在复眼着色期,经4℃冷藏24小时后,可被Ac NPV(苜蓿银纹夜蛾核多角体病毒)感染。在蛹体内复制出的多角体大小差异较大,且比在昆虫Sf—21细胞中繁殖的Ac NPV和在蚕蛹体内形成的Bm NPV多角体小。在蛹体内繁殖的Ac NPV的游离病毒可以回返感染Sf—21细胞。由蚕蛹内分离的Ac NPV基因组DNA的限制性内切酶图谱与野生型的Ac NPV相同。以上结果证明Ac NPV可以在蚕蛹体内复制。含HBsAg基因(人乙肝表面抗原)的重组Ac NPV亦可感染家蚕蛹,并能正确表达外源基因。此外,还发现化蛹后第2天的家蚕蛹被注射约5×10~5PFU的Ac NPV,可诱导“人工滞育蛹”现象的发生,在25℃经过30天后蛹仍存活,发育停滞在复眼着色前阶段。 相似文献