全文获取类型
收费全文 | 155篇 |
免费 | 12篇 |
专业分类
林业 | 19篇 |
农学 | 5篇 |
23篇 | |
综合类 | 17篇 |
农作物 | 9篇 |
水产渔业 | 13篇 |
畜牧兽医 | 66篇 |
园艺 | 3篇 |
植物保护 | 12篇 |
出版年
2023年 | 1篇 |
2021年 | 3篇 |
2020年 | 7篇 |
2019年 | 9篇 |
2018年 | 10篇 |
2017年 | 10篇 |
2016年 | 4篇 |
2015年 | 7篇 |
2014年 | 3篇 |
2013年 | 9篇 |
2012年 | 7篇 |
2011年 | 9篇 |
2010年 | 3篇 |
2009年 | 5篇 |
2008年 | 9篇 |
2007年 | 8篇 |
2006年 | 7篇 |
2005年 | 3篇 |
2004年 | 4篇 |
2003年 | 8篇 |
2002年 | 9篇 |
2001年 | 4篇 |
2000年 | 5篇 |
1999年 | 7篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1996年 | 2篇 |
1995年 | 1篇 |
1992年 | 1篇 |
1991年 | 1篇 |
1990年 | 1篇 |
1989年 | 1篇 |
1987年 | 1篇 |
1970年 | 3篇 |
1968年 | 1篇 |
1967年 | 1篇 |
1966年 | 1篇 |
排序方式: 共有167条查询结果,搜索用时 31 毫秒
31.
Selenium is essential for normal mammalian development. Being a component of antioxidant enzyme, glutathione peroxidase, it plays a major role in protecting the cells from free radical damage. The level of glutathione peroxidase was directly related to the amount of selenium present in various tissues and organs. A decrease in selenium leads to various pathological changes in humans as well as in various laboratory animals. The aim of the present study was to understand whether there is an increase in the level of selenium in different brain regions of rat pups whose mothers were supplemented with selenium, either 2 or 4 mg/l of their drinking water throughout the period of their pregnancy. There was a significant increase in the level of selenium in the cerebellum, cortex and hypothalamic and hippocampal tissues of selenium supplemented mothers as compared with those of non-supplemented mothers. The brain stem of these animals did not show any significant difference in the level of selenium. Furthermore, the differences in the level of selenium between the rat pups of 2 mg/l selenium supplemented mothers and 4 mg/l selenium supplemented mothers were not statistically significant. These studies suggest that supplementation of selenium to mothers during the period of their pregnancy can selectively increase the level of this trace element in different brain regions. Further studies are necessary to understand the significance of selective accumulation of selenium in specific brain regions on brain development and function. 相似文献
32.
Deletion of the M063 gene from myxoma virus produces a virus that is unable to replicate in rabbit cells in vitro or in live rabbits but can be propagated in non-rabbit cell lines. A targeted M063 deletion mutant was constructed in the attenuated Uriarra strain of myxoma virus and the ability of this virus to act as a safe, non-transmissible vaccine against myxomatosis was tested in outbred laboratory rabbits. Immunization with the M063 deletion vaccine provided good short-term protection against lethal challenge with virulent myxoma virus. Long-term protection was similar to reported results with heterologous live virus, with some rabbits protected but others succumbing to challenge. Replication-deficient poxvirus vaccines, like the Modified Vaccinia Virus Ankara (MVA) in man and the myxoma virus vaccine described here in rabbits, are very attractive from a safety perspective. Seasonal boosting would be predicted to provide long-term protection. Targeted host-range gene deletions could have potential for rapid development of poxvirus vaccines in general. 相似文献
33.
34.
35.
36.
Panciera RJ Mathew JS Cummings CA Duffy JC Ewing SA Kocan AA 《Veterinary pathology》2001,38(4):422-426
American canine hepatozoonosis is caused by Hepatozoon americanum, a recently described species of apicomplexan protozoan parasite. An immunohistochemical procedure using a polyclonal antibody to sporozoites of H. americanum clearly identified asexual stages of H. americanum in canine striated muscle. The method also detects hepatozoa present in naturally infected coyotes and raccoons and reacts with certain other apicomplexans. Use of this immunohistochemical procedure confirms the canine intermediate host-parasite relationships that were presumptively established using conventional histopathologic methods. 相似文献
37.
Biofilm formation is one of the factors responsible for antibiotic resistance. The involvement of biofilm formation in bacterial mastitis is well known. Milk composition varies during the lactation period and certain pathogens are producing more number of mastitis cases during particular periods of lactation. The present study elucidates the effects of different milk components on biofilm formation and the persistence of infection. The Plackett Burman screening design has been chosen for assessing the significance. Biofilm production of Staphylococcus aureus, Escherichia coli and Pseudomonas aeruginosa were assessed by crystal violet assay. Dipotassium hydrogen phosphate had a significant effect on biofilm formation by S. aureus (MTCC 1430) whereas it was pH in the case of biofilm formation by P. aeruginosa (NCIM 5029). Other independent factors were found to be insignificant. 相似文献
38.
39.
Stepp SE Dufourcq-Lagelouse R Le Deist F Bhawan S Certain S Mathew PA Henter JI Bennett M Fischer A de Saint Basile G Kumar V 《Science (New York, N.Y.)》1999,286(5446):1957-1959
Familial hemophagocytic lymphohistiocytosis (FHL) is a rare, rapidly fatal, autosomal recessive immune disorder characterized by uncontrolled activation of T cells and macrophages and overproduction of inflammatory cytokines. Linkage analyses indicate that FHL is genetically heterogeneous and linked to 9q21.3-22, 10q21-22, or another as yet undefined locus. Sequencing of the coding regions of the perforin gene of eight unrelated 10q21-22-linked FHL patients revealed homozygous nonsense mutations in four patients and missense mutations in the other four patients. Cultured lymphocytes from patients had defective cytotoxic activity, and immunostaining revealed little or no perforin in the granules. Thus, defects in perforin are responsible for 10q21-22-linked FHL. Perforin-based effector systems are, therefore, involved not only in the lysis of abnormal cells but also in the down-regulation of cellular immune activation. 相似文献
40.
Yared Semahegn Hussein Shimelis Mark Laing Isack Mathew 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2020,70(6):474-484
ABSTRACT Drought is a major factor threatening crop production worldwide. Developing wheat varieties that are adapted to drought prone environments is a sustainable strategy to improve wheat production and productivity. The aim of this study was to evaluate and select bread wheat genotypes for yield and yield components, and for stability under drought stress and non-stress conditions. One hundred and twenty genotypes were evaluated at five test sites in the 2018/19 cropping season using a 10 x 12 alpha lattice design with two replicates. The level of drought stress was imposed using different sowing dates (early planting representing non-stressed, while late planting as drought stressed conditions) following the onset of the main rain at each site. Grain yield and yield components were recorded, and drought indices were calculated for each genotype. Among the drought tolerance indices, GMP, MP, HM, STI and YI were found to be the most suitable for predicting drought tolerance because they had significant and positive correlations with yield under drought stress and non-stress conditions. Rank sum analysis identified the most drought tolerant genotypes as ‘YS-34', ‘YS-85' and ‘YS-82’. The selected wheat genotypes are useful genetic resources for future drought tolerance breeding programmes in Ethiopia or similar agro-ecologies. 相似文献