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排序方式: 共有320条查询结果,搜索用时 15 毫秒
151.
Huang L Ikejiri A Shimizu Y Adachi T Goto Y Toyama J Tanaka H Akashi R Uchida K Miyata H Haga T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(5):533-535
The aim of this study is to investigate the immunoadjuvant activity of the crude Momordica charantia lectin (crMCL) extracted from seed using beta-galactosidase (beta-gal) as the model antigen. BALB/c mice were injected intramuscularly with beta-gal alone or beta-gal + crMCL for up to four immunizations at two-week intervals. After administration of 2 doses, the IgG-specific titer to beta-gal was significantly higher in mice in the beta-gal + crMCL group than in that from the animals from the beta-gal alone group, while it was about the same in both groups after 1 dose. Our data suggest that crMCL may help raise antibodies under the prime and boost administration regimen and could be a potent vaccine adjuvant. 相似文献
152.
Weina Jiang Satoshi Tan Yusuke Hanaki Kazuhiro Irie Hajime Uchida Ryuichi Watanabe Toshiyuki Suzuki Bryan Sakamoto Michiya Kamio Hiroshi Nagai 《Marine drugs》2014,12(12):5788-5800
The toxin-producing cyanobacterium, Moorea producens, is a known causative organism of food poisoning and seaweed dermatitis (also known as “swimmer’s itch”). Two new toxic compounds were isolated and structurally elucidated from an ethyl acetate extract of M. producens collected from Hawaii. Analyses of HR-ESI-MS and NMR spectroscopies, as well as optical rotations and CD spectra indicated two new lyngbyatoxin derivatives, 2-oxo-3(R)-hydroxy-lyngbyatoxin A (1) and 2-oxo-3(R)-hydroxy-13-N-desmethyl-lyngbyatoxin A (2). The cytotoxicity and lethal activities of 1 and 2 were approximately 10- to 150-times less potent than lyngbyatoxin A. Additionally, the binding activities of 1 and 2 possessed 10,000-times lower affinity for the protein kinase Cδ (PKCδ)-C1B peptide when compared to lyngbyatoxin A. These findings suggest that these new lyngbyatoxin derivatives may mediate their acute toxicities through a non-PKC activation pathway. 相似文献
153.
To examine how the number of rock blocks affects the rock-mass runout distance, large-scale outdoor rockfall experiments were physically performed using cubiform granite rock blocks on a slope prepared with granite slabs under both dry and water-saturated conditions. To learn more about the runout mechanism, numerical simulations were also conducted using three-dimensional DEM to reproduce the physical experiments under dry conditions. Longitudinal rock-mass runout distance between the gravity centre in the initial rock block assembly before failure and the apparent gravity centre at final deposition was 10 % larger for experiments under water-saturated conditions than those under dry conditions, with identical numbers of rock blocks in the physical experiments. The physical and numerical experiments revealed that rock blocks at the front and top surfaces had a longer runout distance than those at the rear and bottom surfaces. A rock block scarcely surpassed the rock blocks in front longitudinally and the rock blocks next to it laterally. The equivalent coefficient of friction between gravity centres was positively correlated with the number of rock blocks, which contradicts the findings of a negative correlation between the volume of sturzstroms (rockfall avalanches) and the equivalent coefficient of friction in classic studies on the long runout mechanisms. Our results were likely attributable to the fact that more kinetic energy was dissipated due to repeated inelastic intercollisions with other surrounding rock blocks and granite slabs when the initial rock-mass volume (number of rock blocks) was larger. 相似文献
154.
Suzuki K Ishida M Kadowaki H Shibata T Uchida H Nishida A 《Journal of animal science》2006,84(8):2026-2034
This study estimated genetic parameters for fatty acids of different sites of fat tissue, meat production, and meat quality traits of Duroc pigs selected during 7 generations for ADG, LM area, backfat thickness (BF), and intramuscular fat (IMF). For this study, 394 barrows and 153 gilts were slaughtered at 105 kg of BW. High heritabilities for C18:0 of outer and inner subcutaneous fat tissue were estimated, respectively, as 0.54 and 0.51; those of intermuscular and intramuscular fat were 0.40 and 0.51, respectively. Genetic and phenotypic correlations of ADG and BF with saturated fatty acids of outer and inner subcutaneous fat were positive, but those with C16:1 and C18:2 were negative, and those with C18:1 were nearly zero. Genetic and phenotypic correlations between LM area and respective fatty acids showed opposite results. Respective genetic and phenotypic correlations of melting points with C18:0 and C18:1 were positive and high, and negative and high, respectively. Genetic correlations between cooking loss and SFA (C14:0, C16:0, and C18:0) of IMF were positive and moderate: 0.56, 0.47, and 0.47, respectively. On the other hand, monosaturated fatty acid of C18:1 was highly and negatively correlated with cooking loss (-0.61). Moreover, high genetic correlation between meat color (pork color standard and lightness) and fatty acid compositions of IMF suggest that the SFA (C14:0, C16:0, and C18:0) were correlated genetically with meat lightness and that unsaturated fatty acid compositions (C18:1 and C18:2) were correlated with meat darkness. Results of this study suggest that the fatty acid composition of adipose tissue is correlated genetically with meat production and meat quality traits. 相似文献
155.
156.
Qing Feng Yasuyoshi Torii Koji Uchida Yoshimasa Nakamura Yokihiko Hara Toshihiko Osawa 《Journal of agricultural and food chemistry》2002,50(1):213-220
Tea polyphenols have been demonstrated as chemopreventive agents in a number of experimental models. However, less is known about the mechanism of chemoprevention by black tea compared with that of green tea. Some beneficial properties of theaflavins, the black tea polyphenols, were investigated in the present study. Theaflavins showed inhibitory effects on H(2)O(2)- and tert-butyl hydroperoxide (tBuOOH)-induced cytotoxicity (evaluated by tetrazolium bromide reduction), cellular oxidative stress (detected by oxidation of 2', 7'-dichlorofluorescin), and DNA damage (measured by amount of 8-OHdG and comet assay) in rat normal liver epithelium cell RL-34 cell lines. In addition, theaflavins also exhibited suppression of cytochrome P450 1A1 induced by omeprazole in the human hepatoma HepG2 cell line. Furthermore, when HepG2 cells were pretreated with omeprazole to induce CYP1A1, then exposed to benzo[a]pyrene (B[a]P), DNA damage was observed using the comet assay. However, theaflavins could inhibit this DNA damage. These results indicated that theaflavins could prevent cellular DNA damage by inhibiting oxidative stress and suppressing cytochrome P450 1A1 in cell cultures. 相似文献
157.
158.
The activity of lecithin: cholesterol acyltransferase (LCAT), which is responsible for esterification of plasma cholesterol, was evaluated in bovine serum. It was associated with the high-density lipoprotein fraction that contains apolipoprotein A–I, an activator of LCAT. In lactating cows, the activity was around 1000 U (decrease in nmol of free cholesterol per h per ml of serum), slightly higher than in 1-month-old calves. LCAT activity decreased around parturition, at which the time the serum concentrations of cholesteryl esters and free cholesterol were concomitantly decreased. A reduced LCAT activity was also found in cows with fatty livers induced by the administration of ethionine. In the cows with fatty livers, the serum concentration of cholesteryl esters was markedly decreased, whereas that of free cholesterol was only slightly decreased, thereby increasing the free- to esterified-cholesterol ratio. These results suggest that the decrease in LCAT activity may be involved in the reduction in fertility associated with fatty liver because esterification of cholesterol by LCAT is essential for its transport from the liver to peripheral tissues, such as the corpus luteum, and because cholesterol serves as the source of progesterone synthesis in the latter organ.Abbreviations apoA–I
apolipoprotein A–I
- CE
cholesteryl esters
- CM
chylomicron
- FC
free cholesterol
- HDL
high-density lipoprotein
- IDL
intermediate-density lipoprotein
- LCAT
lecithin:cholesterol acyltransferase
- LDL
low-density lipoprotein
- VHDL
very high-density lipoprotein
- VLDL
very low-density lipoprotein 相似文献
159.
Mg/Ca Thermometry in Coral Skeletons 总被引:1,自引:0,他引:1
The magnesium-to-calcium (Mg/Ca) ratio of coral skeletons from Ishigaki Island, Ryukyu Islands, Japan, closely tracked sea surface temperature (SST) over an 8-year period. Measurements were made with the fast technique of inductively coupled plasma-atomic emission spectrometry. The variation of the coral Mg/Ca ratio with SST change is about four times that of the current, widely used coral strontium-to-calcium ratio. The temporal and geographic variation of the seawater Mg2+/Ca2+ ratio probably has little influence on coral Mg/Ca variation. Results indicate that the coral Mg/Ca ratio has the potential to provide fast, precise, high-resolution proxies for past tropical SSTs. 相似文献
160.
Shioda C Uchida K Nakayama H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(8):1109-1111
A one-year-old, female Mexican axolotl (Ambystoma mexicanum) had a rough-surfaced, polypoid, pink tumor mass of approximately 10 mm in diameter in the oral cavity. Histologically, the tumor extended from the ethmoturbinate region and into the oral cavity and had replaced some of the maxillary bone tissue. The tumor mass was composed of a lobular architecture of small round-shaped tumor cells with occasional Flexner-Wintersteiner-like rosette formation. There were no metastatic lesions in the other organs. Immunohistochemically, the tumor cells were partly positive for several neural markers (class III beta-tubulin, S-100 protein, and doublecortin) and intensely positive for an epithelial marker (cytokeratin AE1/AE3). These results suggest that the present tumor originated from neuroectodermal tissue. Considering the location and histological and immunohistochemical features of the tumor, a diagnosis of olfactory neuroblastoma was made. 相似文献