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排序方式: 共有190条查询结果,搜索用时 46 毫秒
61.
N Hirano K Ono 《Zentralblatt für Veterin?rmedizin. Reihe B. Journal of veterinary medicine. Series B》1990,37(10):721-727
The outbreak of wasting disease of nude mice occurred in the laboratory colony of a Pharmaceutical Company. The viruses producing cytopathic effect with syncytium formation were isolated from the wasted nude mice by DBT cells, and were identified as mouse coronavirus by direct immunofluorescence. The nude mouse colony was closed and all the nude mice (about 500) were killed by the reason of disease control. At autopsy about 60% of nude mice showed necrotic hepatitis. By the virus isolation to see the source of contamination, viruses were isolated from the feces of apparently healthy mice of ICR, CDF1, DBA/2 and C3H, and from human cancer cell line stocked in liquid nitrogen. In experimental infection, the isolates produced only mild hepatitis in ICR mice treated with cortisone. By cross-neutralization test, the nude isolate reacted closely with the virus from C3H mice but not with the virus from cancer cell line. The isolates from nude and C3H mice produced experimentally wasting disease with necrotic hepatitis in nude mice. These findings suggest that wasting disease in nude mice might be caused by low-virulent mouse coronavirus shed in feces from C3H mice introduced before the outbreak of disease. 相似文献
62.
Masachika Hirano Nobushige Itaya Isao Ohno Yoshio Fujita Hirosuke Yoshioka 《Pest management science》1979,10(4):291-294
Thirteen pyrethroid-type esters of substituted 1(or 3)-hydroxymethylimidazolidine-2, 4-dione were synthesised and their knockdown activities against houseflies, mosquitoes and cockroaches were examined. Knockdown activities of 2,4-dioxo-1-prop-2-ynylimidazolidin-3-ylmethyl esters in oil solutions were higher than those of known knockdown pyrethroids; three of the compounds also possessed strong knockdown and flushing-out activities against cockroaches. 相似文献
63.
Kawakami E Takemura A Sakuma M Takano M Hirano T Hori T Tsutsui T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(2):133-136
We measured the blood plasma testosterone (T) levels and superoxide dismutase (SOD) and catalase activities in the seminal plasma of the ejaculates of 5 normal (2-5 years old) and 5 asthenozoospermic (AZ-) (3-5 years old) Beagles. Sperm ejaculated by AZ-dogs was incubated for 3 hr in Eagle's MEM only (controls) or Eagle's MEM containing 100 units/ml of SOD or catalase. Sperm motility was examined during incubation. The mean (+/- SE) plasma T level of the AZ-dogs (1.2 +/- 0.2 ng/ml) was significantly lower than in the normal dogs (2.5 +/- 0.2 ng/ml) (P<0.005). The mean (+/- SE) seminal plasma SOD and catalase activities (18.8 +/- 1.9 and 0.5 +/- 0.1 unit/g protein, respectively) were significantly lower in the AZ-dogs than in the normal dog (43.3 +/- 2.5 and 2.2 +/- 0.4 unit/g protein, respectively) (P<0.001 and 0.01, respectively). The motility of sperm incubated in Eagle's MEM containing SOD or catalase was significantly higher than that of control sperm incubated in only Eagle's MEM after 2 or 3 hr of incubation (P<0.05). The results of this study indicate that poor T secretion by the testes and low antioxidant enzyme activities are related to AZ in the dog. 相似文献
64.
The effect of the floor plate on pattern and polarity in the developing central nervous system 总被引:7,自引:0,他引:7
The effect of floor plate on cellular differentiation in the neural tube of quail embryos was examined. In the developing neural tube the floor plate, which consists of specialized neuroepithelial cells, is located in the ventral midline of the neural tube. When Hensen's node was extirpated the floor plate and notochord did not develop, and the normal differentiation of the ventral horn motor neurons and dorsal and ventral roots did not occur. When one side of the neural tube was deprived of notochord, the ventro-dorsal differentiation took place on both sides. However, when one side of the neural tube was deprived of the floor plate, the ventral horn motor neurons and dorsal and ventral roots did not develop on that side. These observations suggest that the floor plate influences motor neuron differentiation and acts as an intrinsic organizer to establish pattern and polarity in the developing nervous system. 相似文献
65.
Saito Y Itagaki S Otsuka Y Kobayashi Y Okumura H Kobayashi M Hirano T Iseki K 《Journal of agricultural and food chemistry》2005,53(15):6100-6104
In clinical, patients usually take many kinds of drugs at the same time. Thus, drug-drug interactions involving transporters can often directly affect the therapeutic safety and efficacy of many important drugs. However, there have been few studies on food-drug interactions involving transporters. Dietary polyphenols have been widely assumed to be beneficial for human health. Polyphenols are found ubiquitously, and they are commercially prepared and used as functional foods. We have reported that ferulic acid, which is one of the most well-known polyphenols and is used as a functional food, affected the transport of nateglinide, an antidiabetic drug, by Caco-2 cells. In this study, we investigated the effects of other polyphenols on the nateglinide/H(+) transport system. We report here that caffeic acid and p-coumaric acid have a different inhibitory manner on the uptake of nateglinide. The results of this study are useful to identify the substrate specificity of the nateglinide/H(+) cotransporter. 相似文献
66.
Aiko Nakano Hidetoshi Ikeno Toshifumi Kimura Hiromichi Sakamoto Masako Dannoura Yasuhiro Hirano Naoki Makita Leena Finér Mizue Ohashi 《植物养料与土壤学杂志》2012,175(5):775-783
Information related to the growth of fine roots is important for understanding C allocation in trees and the mechanisms of C cycling in ecosystems. Observations using a camera or scanner embedded in the soil enabled us to obtain continuous images of fine‐root‐growth dynamics. However, these methods are still labor‐intensive because the image analysis has to be conducted manually. We developed an automated method for tracking movement or elongation of fine roots using a sequence of scanner images. We also show how data obtained with these methods can be used for calculating fine‐root behavior. Two A4‐size scanners were buried in a mixed forest in Japan and images were taken continuously from within the soil. We preprocessed these images by extracting the fine‐root area from the images and developed an automated calculation plug‐in we named A‐root for tracking growth movement of the tips of fine roots. A‐root and manual‐tracking results were compared using the same images. The results show the A‐root and manual‐tracking methods yielded similar levels of accuracy. The average growth rate of 17 fine roots tracked using the program was 0.16 mm h–1. The observation of the direction of growth in fine roots showed the direction may be influenced by the original root's growth where the fine roots branched, distribution of soil particles, other roots, and the force of gravity. The A‐root analysis also suggested there may be an interaction between speed of growth and changes in direction of growing fine roots. 相似文献
67.
68.
Narihito Ogawa Ryoya Imaizumi Tatsuya Hirano Jun Suzuki 《Journal of Pesticide Science》2021,46(3):278
We investigated the synthesis and herbicidal activity of 23 toxoflavin analogs, 1a–w, in which aromatic rings (R) were introduced into the C-3 position. In paddy field conditions, 1k (R=2-CF3–C6H4) and 1w (R=2-thienyl) showed excellent herbicidal activity. Under upland field conditions, we found that toxoflavin analogs 1a (R=C6H5), 1n (R=2-CH3O–C6H4), and 1p (R=4-CH3O–C6H4) exhibited wide herbicidal spectrum against Echinochloa crus-galli (L) var. crus-galli (ECHCG), Chenopodium album, and Amaranthus viridis (AMAVI). The analog with the 2-fluoro group on benzene ring 1b also showed high herbicidal activity against both ECHCG and AMAVI. 相似文献
69.
Keigo Inami Chizu Yoshioka Yasushi Hirano Masato Kawabe Seiya Tsushima Tohru Teraoka Tsutomu Arie 《Journal of General Plant Pathology》2010,76(2):116-121
Five primer/probe sets to identify the tomato wilt pathogen, Fusarium oxysporum f. sp. lycopersici (FOL), and its three races selectively were designed based on the rDNA-intergenic spacer and avirulence genes. Real-time PCR using
genomic DNA from mycelia and soil DNA with the primer/probe sets allowed the successful identification of FOL and its races. 相似文献
70.
Osmoregulatory actions of growth hormone and its mode of action in salmonids: A review 总被引:8,自引:0,他引:8
Tatsuya Sakamoto Stephen D. McCormick Tetsuya Hirano 《Fish physiology and biochemistry》1993,11(1-6):155-164
Osmoregulatory actions of growth hormone (GH) and its mode of action in salmonids are reviewed. We present evidence suggesting
that insulin-like growth factor I (IGF-I) mediates some of the actions of GH on seawater acclimation. Plasma concentration
and turnover of GH rise following exposure to seawater. Exogenous GH (in vivo) increases gill Na+,K+-ATPase activity and the number of gill chloride cells, and inhibits an increase in plasma osmolarity and ions following transfer
of fish to seawater. A single class of high affinity GH receptors is present in the liver, gill, intestine, and kidney. The
levels of IGF-I mRNA in the liver, gill and kidney increased after GH-injection. After transfer to seawater, IGF-I mRNA increased
in the gill and kidney following the rise in plasma GH, although no significant change was seen in the liver. Injection of
IGF-I improved the ability of the fish to maintain plasma sodium levels after transfer to seawater. GH treatment also sensitizes
the interrenal to adrenocorticotropin (ACTH), increasing cortisol secretion. Both cortisol and IGF-I may be involved in mediating
the action of GH in seawater adaptation, although studies on the effect of GH on osmoregulatory physiology of non-salmonid
species are limited. An integrated model of the osmoregulatory actions of GH is presented, and areas in need of research are
outlined.
Résumé Cet article est une revue des effets osmorégulateurs de l'hormone de croissance et de son mode d'action. Nous présentons des résultats qui suggèrent que le facteur de croissance de type insuline (IGF-I) est un médiateur de certaines des actions de la GH sur l'adaptation à l'eau de mer. Les concentrations plasmatiques et le renouvellement de la GH augmentent après transfert en eau de mer. La GH exogène stimule (in vivo) l'activité Na+,K+-ATPase et le nombre de cellules à chlorure branchialeset inhibe les augmentations de l'osmolarité et des concentrations ioniques du plasma observées après transfert en eau de mer. Une seule classe de récepteurs à haute affinité pour la GH est présent dans le foie, les branchies, l'intestin et le rein. Les niveaux d'ARNm d'IGF dans le foie, les branchies et le rein augmentent après injection de la GH. Après transfert en eau de mer, les ARNm de l'IGF augmentent dans les branchies et dans le rein en suivant l'augmentation de GH plasmatique, bien qu'aucune modification ne soit observée au niveau du foie. L'injection d'IGF augmente la capacité du poisson à maintenir ses niveaux de sodium plasmatique après transfert en eau de mer. Le traitement à la GH augmente la sensibilité à l'adrenocorticotropine (ACTH) et stimule donc les niveaux de cortisol. A la fois le cortisol et l'IGF-I semblent impliqués comme médiateurs des effets de la GH dans l'adaptation à l'eau de mer, bien que les études sur les effets de la GH sur la physiologie de l'osmorégulation chez les espèces non-salmonidés restent encore limitées. Un modèle intégré des actions de la GH sur l'osmorégulation est présenté et les domaines de recherche à développer sont soulignés.相似文献