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251.
Lesley G. King MVB MRCVS Martha T. Stevens BSc Ernest N. So. Ostro Deborah Diserens M. Phil Ravi Shankar J. MS 《Journal of Veterinary Emergency and Critical Care》1994,4(2):85-99
A system was developed for objective prediction of survival, which could be applied to all critically ill patients, regardless of disease, at an early stage of hospitaluation. Such a system would allow risk assessment of groups for experimental studies according to probability of survival and it might allow us to avoid expenditure of scarce clinical resources on animals with little chance of survival. The prospective study included 200 critically ill dogs: 93 females and 107 males, representing 62 breeds. With survival defined as alive 30 days after admisssion to ICU the overall mortality rate was 40.5% (81 of 200 dogs). Data collected included signalment and parameters that reflected vital organ function, the severity of physiologic derangement and the extent of physiologic reserve. We recorded the most abnormal value for each parameter within 24 hours of admission to ICU and logistic regression analysis was used to analyze four different weighting systems. The best model had a concordance 0f 86.5% with outcome, and it was then re-evaluated to determine whether individual variables could be eliminated without losing predictive accuracy. Fourvariables were eliminated, resulting in a final model with 18 variables which had 86.3% concordance with outcome. At a 0.5 cut-off for predicted risk, the model had sensitivity Of 69%, specificity ficity of 86% and positive predictive value of 77%. A Receiver Operating Curve was constructed using serial cut-offs for predicted outcome from 0.1 to 0.9, and the area under the curve was 0.89. Thus, an equation was generated that gives an estimate of the probability of survival. 相似文献
252.
Donald L Neiffer Michele A Miller Martha Weber Mark Stetter Deidre K Fontenot P K Robbins Geoffrey W Pye 《Journal of zoo and wildlife medicine》2005,36(2):250-256
Standing sedation was provided for 14 clinical procedures in three African elephants (Loxodonta africana) managed by combined protected and modified-protected contact and trained through operant conditioning. An initial hand-injection of detomidine hydrochloride and butorphanol tartrate at a ratio of 1:1 on a microg:microg basis was administered intramuscularly, with a dosage range of 50-70 mg (12.9-19.7 microg/kg) for each drug. The initial injection resulted in adequate sedation for initiation and completion of eight procedures, whereas supplemental doses were required for the remaining procedures. The dosage range for the supplemental injections of each drug was 4.0-7.3 microg/kg. Initial effect was noted within 3.0-25 min (mean = 11.6 min, SD +/- 5.9 min), with maximal effect occurring at 25-30 min for those procedures not requiring supplementation. In all but one procedure, this effect was maintained until the end of the procedure, which ranged from 47 to 98 min (mean = 74.7 min, SD +/- 18.8 min). No cardiac or respiratory depression was appreciated. Recovery after administration of reversal agents was rapid and complete, ranging from 2 to 20 min (mean = 9.0 min, SD +/- 7.0 min). On the basis of the authors' experience, recommended dosage ranges for reversal agents would be intravenous yohimbine (73.4-98.5 microg/kg), intravenous naltrexone (48.9-98.5 microg/kg), and intramuscular naltrexone (73.4-98.5 microg/kg). Approximately one-third to one-half of the total naltrexone dose should be administered intravenously. Mild adverse side effects limited to the gastrointestinal tract were observed in association with five procedures including abdominal distention with or without transient anorexia. Administration of reversal agents, encouraging exercise and water consumption, and administration of flunixin meglumine were helpful in the resolution of signs. In addition to gastrointestinal signs, slight ataxia was observed before initiation of surgical stimulation during one procedure in which 19.7 microg/kg of each drug was administered. On the basis of the procedures that did not require supplementation to initiate treatment and taking into consideration the potential for ataxia at higher doses, a starting dosage range of 14.7-16.2 microg/kg of both detomidine and butorphanol in a ratio of 1:1 on a microg:microg basis administered i.m. simultaneously is recommended. 相似文献
253.
Pablo Pi?eyro Miranda D. Vieson José A. Ramos-Vara Martha Moon-Larson Geoffrey Saunders 《Journal of veterinary science (Suw?n-si, Korea)》2014,15(3):449-453
This report describes the gross, histological, and immunohistochemical features of medullary thyroid carcinoma (MTC) with pulmonary metastases in a young dog. Sheets of pleomorphic cells supported by fibrous stroma characterized the primary mass, while metastatic nodules had a neuroendocrine pattern. Despite differing histologic features, all masses showed marked immunoreactivity against calcitonin and multiple neuroendocrine markers consistent with MTC. Although MTC is a well-recognized entity, it may be difficult to distinguish this mass from other thyroid neoplasms, necessitating immunohistochemical characterization. 相似文献
254.
Martha Maria Passador Patrícia Rodrigues de Lima Cristiane de Pieri Juan Fernan Sierra-Hayer Ricardo Harakava Edson Luiz Furtado 《European journal of plant pathology / European Foundation for Plant Pathology》2013,137(1):137-147
Among the most important diseases affecting Eucalyptus is Mycosphaerella Leaf Disease (MLD) caused by Mycosphaerella spp. and Teratosphaeria spp. MLD has led to significant losses in eucalypt plantations in the South and Southeast Region of Brazil, as well as in several countries such as Portugal, Spain, South Africa and Australia. Symptoms of MLD include localized necrotic spots, early defoliation in juvenile plants, stem cankers, early death of branches, and in some cases, atrophy and death. In the present study, single spore isolations from leaves of E. globulus from five locations in Brazil allowed the differentiation of species of Mycosphaerella and Teratosphaeria based on ascospore germination and growth in culture. These isolates were also subjected to sequence analysis of the ribosomal RNA internal transcribed spacer regions, which allowed their identification to species level. The results of this study showed that six species of Mycosphaerella and four species of Teratosphaeria were associated with leaves showing symptoms of MLD in E. globulus plantations in various locations of Brazil. 相似文献
255.
Priyanka Sahariah Vivek S. Gaware Ramona Lieder Sigríeur Jónsdóttir Martha á. Hjálmarsdóttir Olafur E. Sigurjonsson Már Másson 《Marine drugs》2014,12(8):4635-4658
A series of water-soluble cationic chitosan derivatives were prepared by chemoselective functionalization at the amino group of five different parent chitosans having varying degrees of acetylation and molecular weight. The quaternary moieties were introduced at different alkyl spacer lengths from the polymer backbone (C-0, C-2 and C-6) with the aid of 3,6-di-O-tert-butyldimethylsilyl protection of the chitosan backbone, thus allowing full (100%) substitution of the free amino groups. All of the derivatives were characterized using 1H-NMR, 1H-1H COSY and FT-IR spectroscopy, while molecular weight was determined by GPC. Antibacterial activity was investigated against Gram positive S. aureus and Gram negative E. coli. The relationship between structure and activity/toxicity was defined, considering the effect of the cationic group’s structure and its distance from the polymer backbone, as well as the degree of acetylation within a molecular weight range of 7–23 kDa for the final compounds. The N,N,N-trimethyl chitosan with 100% quaternization showed the highest antibacterial activity with moderate cytotoxicity, while increasing the spacer length reduced the activity. Trimethylammoniumyl quaternary ammonium moieties contributed more to activity than 1-pyridiniumyl moieties. In general, no trend in the antibacterial activity of the compounds with increasing molecular weight or degree of acetylation up to 34% was observed. 相似文献
256.
Himler AG Adachi-Hagimori T Bergen JE Kozuch A Kelly SE Tabashnik BE Chiel E Duckworth VE Dennehy TJ Zchori-Fein E Hunter MS 《Science (New York, N.Y.)》2011,332(6026):254-256
Maternally inherited bacterial symbionts of arthropods are common, yet symbiont invasions of host populations have rarely been observed. Here, we show that Rickettsia sp. nr. bellii swept into a population of an invasive agricultural pest, the sweet potato whitefly, Bemisia tabaci, in just 6 years. Compared with uninfected whiteflies, Rickettsia-infected whiteflies produced more offspring, had higher survival to adulthood, developed faster, and produced a higher proportion of daughters. The symbiont thus functions as both mutualist and reproductive manipulator. The observed increased performance and sex-ratio bias of infected whiteflies are sufficient to explain the spread of Rickettsia across the southwestern United States. Symbiont invasions such as this represent a sudden evolutionary shift for the host, with potentially large impacts on its ecology and invasiveness. 相似文献
257.
258.
Brownlee DE Horz F Newburn RL Zolensky M Duxbury TC Sandford S Sekanina Z Tsou P Hanner MS Clark BC Green SF Kissel J 《Science (New York, N.Y.)》2004,304(5678):1764-1769
Images taken by the Stardust mission during its flyby of 81P/Wild 2 show the comet to be a 5-kilometer oblate body covered with remarkable topographic features, including unusual circular features that appear to be impact craters. The presence of high-angle slopes shows that the surface is cohesive and self-supporting. The comet does not appear to be a rubble pile, and its rounded shape is not directly consistent with the comet being a fragment of a larger body. The surface is active and yet it retains ancient terrain. Wild 2 appears to be in the early stages of its degradation phase as a small volatile-rich body in the inner solar system. 相似文献
259.
Wildlife conservationists and agencies have recommended managing rangelands for vegetation heterogeneity to improve wildlife habitat, particularly for many grassland birds. However, range management focuses on livestock production and associated practices and structural items (fences, water developments) that are applied to improve livestock distribution. This article assesses the occurrence of livestock distribution–oriented grazing management in the spending and applied practices of the Natural Resources Conservation Service (NRCS), the federal agency charged with assisting private landowners with implementation of US Department of Agriculture conservation programs. NRCS applied practices and spending both suggest an emphasis on livestock distribution and associated structures, with almost 20 million ha of prescribed grazing systems, 10 000 km of fence, and 127 000 water facilities implemented in 17 western states with assistance from NRCS programs between 2004 and 2007. Ninety percent of funding for 2005 Conservation Security Program grazing enhancements supported investments or management related to livestock distribution. We suggest that increasing the emphasis of NRCS conservation programs and financial assistance on maintaining or increasing compositional and structural heterogeneity of vegetation, rather than on livestock distribution, could be an approach that unifies livestock production and wildlife habitat objectives. In particular, including vegetation heterogeneity as a central goal for upland wildlife habitat management could be a key opportunity to increase incentives for heterogeneity-promoting management on privately owned rangeland. 相似文献
260.
Patricia Davenport Viola Lorenz Zhi-Jian Liu Henry A. Feldman Jorge Canas Emily Nolton Chiara-Aiyleen Badur Thi Minh-Thi Do Martha Sola-Visner 《Journal of veterinary diagnostic investigation》2021,33(5):913
The immature platelet fraction (IPF) is a measure of newly released platelets, which has been used as a marker of platelet production in multiple human studies but is not widely available in multispecies analyzers. We developed gates to measure the IPF in diluted and undiluted murine blood samples on the Sysmex XN-1000V multispecies hematology analyzer. IPF gates were created using undiluted and diluted (1/10) blood samples obtained from adult and newborn (postnatal day 10, P10) C57BL/6J wild-type (WT) mice, and from 3 murine models of thrombocytopenia: c-MPL−/− mice, which lack the thrombopoietin receptor (hyporegenerative); antibody-mediated thrombocytopenia; and acute inflammation-induced thrombocytopenia. P10 mice were chosen because, at their size, we could consistently obtain (by terminal phlebotomy) the blood volume needed to run an undiluted sample. The undiluted blood IPF gate successfully differentiated between mechanisms of thrombocytopenia in both adult and P10 mice. For diluted samples, 2 IPF gates were generated: a thrombocytopenic (T) gate, which performed well in samples with platelet counts (PCs) <800 × 109/L in adult mice and <500 × 109/L in newborn mice, and a non-thrombocytopenic (NT) gate, which performed well in samples with PCs above these thresholds. PCs and IPFs measured in diluted blood using these gates agreed well with those measured in undiluted blood and had good reproducibility. These diluted gates allow for the accurate measurement of PCs and IPFs in small (10 µL) blood volumes, which can be obtained easily from adult and newborn mice as small as P1 to assess platelet production serially. 相似文献