Camera steered mechanical weed control in sugar beet,maize and soybean

In sugar beet, maize and soybean, weeds are usually controlled by herbicides uniformly applied across the whole field. Due to restrictions in herbicide use and negative side effects, mechanical weeding plays a major role in integrated weed management (IWM). In 2015 and 2016, eight field experiments were conducted to test the efficacy of an OEM Claas 3-D stereo camera^® in combination with an Einböck Row-Guard^® hoe for controlling weeds. Ducks-foot blades in the inter-row were combined with four different mechanical intra-row weeding elements in sugar beet, maize and soybean and a band sprayer in sugar beet. Average weed densities in the untreated control plots were from 12 to 153 plants m^?2 with Chenopodium album, Polygonum convolvulus, Thlapsi arvense being the most abundant weed species. Camera steered hoeing resulted in 78% weed control efficacy compared to 65% using machine hoeing with manual guidance. Mechanical intra-row elements controlled up to 79% of the weeds in the crop rows. Those elements did not cause significant crop damage except for the treatment with a rotary harrow in maize in 2016. Weed control efficacy was highest in the herbicide treatments with almost 100% followed by herbicide band-applications combined with inter-row hoeing. Mechanical weed control treatments increased white sugar yield by 39%, maize biomass yield by 43% and soybean grain yield by 58% compared to the untreated control in both years. However, yield increase was again higher with chemical weed control. In conclusion, camera guided weed hoeing has improved efficacy and selectivity of mechanical weed control in sugar beet, maize and soybean.     

Efficacy of copper alternatives applied as stop-sprays against Plasmopara viticola in grapevine

Journal of Plant Diseases and Protection - The six products, Armicarb, Vitisan, lime sulphur, Caso, Calce Fiocco and Ulmasud, were tested for their ability to control downy mildew (Plasmopara...     

Chlorogenic acid,a metabolite identified by untargeted metabolome analysis in resistant tomatoes,inhibits the colonization by Alternaria alternata by inhibiting alternariol biosynthesis

Tomato fruits can be contaminated by saprophytic strains of Alternaria alternata which is the reason for the frequent occurrence of Alternaria toxins like alternariol, alternariol monomethylether or tenuazonic acid in these types of products. It was shown earlier that alternariol is a colonization factor for tomatoes. In the current analysis two different tomato genotypes were analysed by untargeted comprehensive two-dimensional gas chromatography mass spectrometry (GC×GC-MS). This analysis revealed clear differences in the metabolic profiles which were paralleled by differences in resistance towards Alternaria colonization. One of the genotypes was more resistant against A. alternata infection and contained high amounts of chlorogenic acid in contrast to the other genotype which was sensitive against infection. In in vitro analysis, chlorogenic acid reduced alternariol biosynthesis during the first days of growth of A. alternata. Expression analysis of the alternariol polyketide synthase gene, a key gene in the biosynthesis of alternariol, also revealed a temporal reduction in its expression in the first phases of growth. However by chromatographic analysis it could be demonstrated that chlorogenic acid was degraded over time. This degradation leads to a relief of inhibition resulting in an only temporal inhibition of alternariol biosynthesis. In vivo colonization experiments revealed that chlorogenic acid reduces colonization of tomatoes by A. alternata in a concentration dependent manner, which however is partly counteracted by the addition of alterariol.     

Species-specific barrier effects of a motorway on the habitat use of two threatened forest-living bat species

It is generally recognized that roads can adversely affect local animal populations but little is known how roads affect bats. In particular, no study compared the response of bats that differ in foraging ecology to motorways that cut through the breeding habitat. As bats are key species in conservation, such data are urgently needed for designing management plans. Using radio-telemetry, mist netting, and mark-recapture data we investigated the effects of a motorway with heavy traffic on the habitat use of two threatened forest-living bats. We compared barbastelle bats (Barbastella barbastellus), which forage in open space, to Bechstein’s bats (Myotis bechsteinii), which glean prey from the vegetation. Five of six radio-tracked barbastelle bats crossed the motorway during foraging and roost switching, flying through underpasses and directly over the motorway. In contrast, only three of 34 radio-tracked Bechstein’s bats crossed the motorway during foraging, all three using an underpass. Bechstein’s bats, unlike barbastelle bats, never crossed the motorway during roost switching. Moreover, only in Bechstein’s bats individuals foraging close to the motorway had smaller foraging areas than individuals foraging further away, whereas other forest edges had no such effect. Our data show that motorways can restrict habitat accessibility for bats but the effect seems to depend on the species’ foraging ecology and wing morphology. We suggest that motorways have stronger barrier effects on bats that forage close to surfaces than on bats that forage in open space, and discuss the implications of our findings for bat conservation during road construction.     

Bioaccumulation of ivermectin from natural and artificial sediments in the benthic organism <Emphasis Type="Italic">Lumbriculus variegatus</Emphasis>

Purpose  

Although ivermectin is a widely used lipophilic parasiticide, data on its potential bioaccumulation in aquatic invertebrates are scarce. In this study, bioaccumulation patterns of radiolabeled ³H-ivermectin from sediments into tissues of the sediment-dwelling worm Lumbriculus variegatus were investigated and assessed.     

Effect of tree-level airborne laser-scanning measurement accuracy on the timing and expected value of harvest decisions

The objective was to compare tree-level airborne laser-scanning (ALS) data accuracy with standwise estimation data accuracy as input data for forest planning, using tree- and stand-level simulators. The influence of the input data accuracy was studied with respect to (1) timing of the next thinning or clear-cutting and (2) the relative variation in the predicted income of the next logging expressed as the net present value (NPV). The timing and predicted NPV of thinning and clear-cutting operations were considered separately. The research was based on Monte Carlo simulations carried out with the tree- and stand-level simulators using a simulation and optimisation (SIMO) framework. The simulations used treewise measurements taken on 270 circular plots measured at the Evo Field Station, Finland, as input data. Deviations in the tree data measured were generated according to the mean standard errors found in standwise field estimation and tree-level ALS. The accuracy factors of ALS individual tree detection were based on the EUROSDR/ISPRS Tree Extraction Project. The results show that input data accuracy significantly affects both the timing and relative NPV of loggings. Tree-level ALS produces more accurate simulation results than standwise estimation with the error levels assumed. Diameter-based characteristics are the most important input data in all simulations. Accurate tree height estimates cannot be fully utilised in current simulators.     

Phosphorus release from forest harvesting on an upland blanket peat catchment

The aim of this study was to investigate the release of phosphorus (P) to receiving waters resulting from harvesting 34-year-old lodgepole pine trees in an upland peat catchment. The study site was within a 25.3-hectare (ha) area, and was drained by a stream that received flows from ploughed furrows, mainly, via collector drains, and discharged directly to the salmonid Shrahrevagh River, Burrishoole, Co. Mayo, Ireland. The study site was divided into two parts: the upstream part was left intact and the downstream part was harvested in early Autumn 2005 following implementation of forest guidelines. Good management practices such as proper use of brash mats and harvesting only in dry weather were implemented. Two instrumented stations were established – one just upstream (US) and the other just downstream (DS) of the clearfelled area. The measurement of P concentrations at the two stations commenced in May 2005, two months before the harvesting started. The daily mean P concentration at the DS station increased from about 6 μg L⁻¹ of total reactive phosphorus (TRP) during pre-clearfelling to 429 μg L⁻¹ in August 2006. By October 2009, four years after clearfelling, the P concentrations at the DS station had returned to pre-clearfelling levels. In the first three years after harvesting, up to 5.15 kg ha⁻¹ of TRP was released from the harvested catchment to the receiving water; in the second year alone, 2.3 kg ha⁻¹ of TRP was released. Linear regression can be used to describe the relationship between TRP load and water discharge. About 80% of the total phosphorus (TP) in the study stream was soluble and more than 70% of the P release occurred in storm events, indicating that traditional buffer strips with widths of 15–20 m might not be efficient for P immobilization. The P concentrations were affected by antecedent weather conditions and highest concentrations occurred during storm events following prolonged drought periods. The water extractable phosphorus (WEP) contents in the soil were significantly higher below windrow/brash material than in brash-free areas, and whole-tree harvesting should be studied as one of the means to decrease P export from blanket peats.     

Use of polymorphisms in the γ-gliadin gene of spelt and wheat as a tool for authenticity control

Partial sequencing of the γ-gliadin gene of 62 spelt and 14 soft wheat cultivars was performed. Fifty-six of the 62 spelt cultivars and 13 of the 14 soft wheat cultivars were shown to exhibit the typical spelt or soft wheat γ-gliadin sequence, respectively. Exceptions were ascribed to crossbreeding of soft wheat and spelt. Using the typical soft wheat γ-gliadin sequence, two alternative DNA-based analytical methods were developed for the detection and quantification of spelt flour "adulteration" with soft wheat. A simple and fast detection of soft wheat in spelt flours could be achieved by restriction fragment length (RFLP) analysis. In combination with lab-on-a-chip capillary gel electrophoresis (LOC-CE) the soft wheat proportion could be estimated. Heteroduplex formation served as additional confirmation for the presence of spelt besides soft wheat. Hence, RFLP-LOC-CE constitutes a perfect analysis tool for the quality control of cereal seeds and pure cultivars. A precise quantification of soft wheat "adulterations" in spelt flour down to 1% could be achieved by the developed real-time PCR method. The calibration parameters of the real-time PCR assay fulfilled the minimum performance requirements of the European Network of GMO (genetically modified organisms) Laboratories (ENGL).