全文获取类型
收费全文 | 543篇 |
免费 | 11篇 |
专业分类
林业 | 67篇 |
农学 | 10篇 |
基础科学 | 4篇 |
219篇 | |
综合类 | 91篇 |
农作物 | 21篇 |
水产渔业 | 15篇 |
畜牧兽医 | 90篇 |
园艺 | 12篇 |
植物保护 | 25篇 |
出版年
2024年 | 1篇 |
2023年 | 5篇 |
2022年 | 7篇 |
2021年 | 14篇 |
2020年 | 7篇 |
2019年 | 12篇 |
2018年 | 27篇 |
2017年 | 12篇 |
2016年 | 12篇 |
2015年 | 17篇 |
2014年 | 14篇 |
2013年 | 25篇 |
2012年 | 43篇 |
2011年 | 37篇 |
2010年 | 26篇 |
2009年 | 19篇 |
2008年 | 43篇 |
2007年 | 26篇 |
2006年 | 30篇 |
2005年 | 30篇 |
2004年 | 25篇 |
2003年 | 24篇 |
2002年 | 31篇 |
2001年 | 10篇 |
2000年 | 4篇 |
1999年 | 4篇 |
1998年 | 5篇 |
1997年 | 2篇 |
1996年 | 2篇 |
1994年 | 3篇 |
1993年 | 1篇 |
1992年 | 3篇 |
1991年 | 6篇 |
1990年 | 2篇 |
1989年 | 2篇 |
1987年 | 1篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1982年 | 1篇 |
1980年 | 1篇 |
1978年 | 3篇 |
1977年 | 2篇 |
1976年 | 1篇 |
1975年 | 1篇 |
1974年 | 2篇 |
1973年 | 2篇 |
1972年 | 1篇 |
1971年 | 3篇 |
1966年 | 1篇 |
1960年 | 1篇 |
排序方式: 共有554条查询结果,搜索用时 328 毫秒
151.
Nitrogen turnover in a loess catena N-mineralization and mineral-N-contents were determined from spring 1993 to autumn 1995 on arable soils from loess (Luvisol, Calcaric Regosol, Gleyic-Calcaric Regosol and Cumulic Anthrosol) of a catchment area called “Bonartshäuser” farm near Gondelsheim in western Kraichgau (SW-Germany). The aim was to find out, whether the course of net-N-mineralization and mineral-N-content of the soil could be explained by the parameters soil temperature and soil moisture. Soil samples were incubated in polyethylene bags on site for mineralization and aliquots analyzed in the laboratory for the determination of the mineral-N-content. In 1993, 55 kg N ha—1 were immobilized and up to 170 kg N ha—1 mineralized depending on the soil type. In 1994, between 181 and 297, and in 1994, between 59 and 230 kg N ha—1 were mineralized annually. Mineral-N-contents of the different soils throughout the experimental period (n = 45) were found to correlate much better (r2 between 0.55 and 0.86) whereas net-N-mineralization (n = 44) showed coefficients of determination (r2) just between 0.08 and 0.53. Except for the Luvisol (37%) only 0 to 8% of net-N-mineralization could be explained by the combined effect of mean soil temperature and soil moisture at the beginning of the incubation using multiple linear regression analysis. Merely 1 up to 9% of mineral-N-content of the soil could be explained by the same effect. The variability and inconsistency shown by net-N-mineralization renders it inappropriate as a tool for predicting nitrogen delivery of the soil and basis for setting rules governing permissible nitrogen amounts in the soil. 相似文献
152.
To simulate a future ion input reduction scenario in forests, a large scale field experiment was set up in a (1999) 66‒year‒old Norway spruce plantation at Solling, central Germany. Throughfall input of H+, SO42—, and N‒compounds is artificially reduced by means of a permanent roof construction below the canopy and a de‒ionizing equipment since 1991. Here we present long term soil solution records for SO42—, NO3—, Al3+ and the pH of the 10 cm mineral soil sampling depth. A significant decrease in ion concentrations since the start of the treatment is observed, but no change of the soil solution pH. Even in the fourth year pH values remained well within the aluminium buffer range (pH < 4.2). Three years after the start of the experiment (July 1994) it was examined whether microbial biomass (Cmic), specific activity (heat production per unit biomass), and the percentage of Cmic in organic C material indicated any changes. Furthermore chemical standard parameters (CEC, base saturation, pH) were analyzed for all soil samples. Results indicate that despite of drastic decreases of soil solution ion concentrations in the upper soil horizons microbial parameters were not affected and that the soil solid phase is not deacidified by the treatment until now. 相似文献
153.
Markus Raubuch Jens Dyckmans Rainer Georg Joergensen Malte Kreutzfeldt 《植物养料与土壤学杂志》2002,165(4):435-440
Temperature, drying, and rewetting are important climatic factors that control microbial properties. In the present study we looked at the respiration rates, adenosine 5′‐triphosphate (ATP) content, and adenylate energy charge (AEC) as a measure for energy status of microbial biomass in the upper 5 cm of mineral soils of three beech forests at different temperatures and after rewetting. The soils differed widely in pH (4.0 to 6.0), microbial biomass C (92 to 916 μg (g DW)—1) and ATP content (2.17 to 7.29 nmol ATP (g DW)—1). The soils were incubated for three weeks at 7 °C, 14 °C, and 21 °C. After three weeks the microbial properties were determined, retaining temperature conditions. The temperature treatment did not significantly affect AEC or ATP content, but respiration rates increased significantly with increasing temperature. In a second experiment the soils were dried for 12 hours at 40 °C. Afterwards the soils were rewetted and microbial properties were monitored for 72 hours. After the drying, respiration rates dropped below the detection limit, but within one hour after rewetting respiration rates increased above control level. Drying reduced AEC by 16 % to 44 % and ATP content by 47 % to 78 %, respectively. Rewetting increased AEC and ATP content significantly as compared to dry soil, but after 72 hours the level of the controls was still not reached. The level of AEC values indicated dormant cells, but ATP content increased. These results indicate that the microbial carbon turnover was not directly linked to microbial growth or microbial energy status. Furthermore our results indicate that AEC may describe an average energy status but does not reflect phases of growing, dormant, or dying cells in the complex microbial populations of soils. 相似文献
154.
155.
Data from the historical River Ems glass eel fisheries in the 20th Century have been used for the ICES recruitment series. Commercial catches ceased from 1980 and dropped to zero; thus, eel recruitment data are since lacking. The present study assessed the actual eel recruitment to the River Ems with regard to the local tidal conditions. Standardised measurements were performed on relative glass eel occurrence at the tidal weir from 2014 and quantitative sampling at the next weir upstream from 2013. Eel migration at the tidal weir was dominated by glass eels and increased 2–3 month earlier than at the upstream weir, where migration was dominated by pigmented eels. In 2016, glass eels were marked at the tidal weir and pigmented elvers were sampled at the upstream weir. The 2016 eel recruitment was estimated at about 1% of the mean historical recruitment. Eel recruitment at the two weirs was not directly linked. Many eels seem not to pass the upstream weir. Further investigations are needed to reveal whether the results represent a regular pattern. In the long run, the perpetuation of the recruitment series is also foreseen. 相似文献
156.
Matti Janhunen Harri Vehvilinen Juha Koskela Antti Forsman Markus Kankainen 《Aquaculture Research》2019,50(3):818-825
Three‐summers‐old all‐female triploid and diploid rainbow trout were compared after one on‐growing season in sea net cages. Slaughter traits of round weight, gutted weight, fillet weight, carcass% and fillet% were measured at three times in November 2017, January and April 2018. The triploid group had lower daily growth coefficient mean (4.25) and higher feed conversion ratio (1.18) than diploids (4.48 and 1.05, respectively) during on‐growing (June–November). In November, no difference of means was found between mature or immature diploids and triploids for any of the weight traits when the effect of vertebrae defects was statistically removed. However, the triploids had attained higher means than mature or immature diploids in gutted and fillet weight by January, suggesting that the loss of muscle mass during early winter was lower in triploids. Sexually maturing diploids (46%) had lower slaughter yield means compared to triploids or immature diploids at each measurement time, and these differences also increased during overwintering. Instead, the means of yield traits remained similar between the triploid and immature diploid groups through the winter. Likewise, fillet redness remained at equally high level in triploids and immature diploids, whereas in maturing diploids this attribute decreased substantially during overwintering. The triploid group had a higher incidence of vertebral defects (12.0%) than diploids (5.3%). The present results demonstrate the potential of triploid trout in producing large‐sized (>2 kg) fillet fish until spring markets. However, more detailed investigations are needed, particularly regarding the animal health and growth efficiency in triploids, relative to their diploid counterparts. 相似文献
157.
Sergey A. Dyshlovoy Moritz Kaune Jessica Hauschild Malte Kriegs Konstantin Hoffer Tobias Busenbender Polina A. Smirnova Maxim E. Zhidkov Ekaterina V. Poverennaya Su Jung Oh-Hohenhorst Pavel V. Spirin Vladimir S. Prassolov Derya Tilki Carsten Bokemeyer Markus Graefen Gunhild von Amsberg 《Marine drugs》2020,18(12)
Efficacy and mechanism of action of marine alkaloid 3,10-dibromofascaplysin (DBF) were investigated in human prostate cancer (PCa) cells harboring different levels of drug resistance. Anticancer activity was observed across all cell lines examined without signs of cross-resistance to androgen receptor targeting agents (ARTA) or taxane based chemotherapy. Kinome analysis followed by functional investigation identified JNK1/2 to be one of the molecular targets of DBF in 22Rv1 cells. In contrast, no activation of p38 and ERK1/2 MAPKs was observed. Inhibition of the drug-induced JNK1/2 activation or of the basal p38 activity resulted in increased cytotoxicity of DBF, whereas an active ERK1/2 was identified to be important for anticancer activity of the alkaloid. Synergistic effects of DBF were observed in combination with PARP-inhibitor olaparib most likely due to the induction of ROS production by the marine alkaloid. In addition, DBF intensified effects of platinum-based drugs cisplatin and carboplatin, and taxane derivatives docetaxel and cabazitaxel. Finally, DBF inhibited AR-signaling and resensitized AR-V7-positive 22Rv1 prostate cancer cells to enzalutamide, presumably due to AR-V7 down-regulation. These findings propose DBF to be a promising novel drug candidate for the treatment of human PCa regardless of resistance to standard therapy. 相似文献
158.
Mikko T. Niemi Mikko Vastaranta Jari Vauhkonen Timo Melkas Markus Holopainen 《Scandinavian Journal of Forest Research》2017,32(8):762-773
Heavy off-road traffic causes soil compaction and rutting, which can significantly reduce the yield of forest stands. Reliable information on terrain trafficability, that is, the ability of terrain to support the passage of vehicles, would enable significant enhancement of wood procurement planning and reduction of soil damage. The objective here was to determine the feasibility of airborne scanning light detection and ranging (LiDAR)-derived digital terrain models (DTM) in terrain trafficability mapping. Soil damage was inventoried from a total of 13?km of forwarding trails, and a logistic regression model was fitted for predicting the event of soil damage. DTM-derived soil wetness indices performed well as predictor variables, and DTM-derived local binary patterns also proved useful in terrain trafficability mapping. A prediction accuracy of 83.6% (Cohen’s kappa of 0.38) was observed for soil damage probability modelling, using only DTM-derived predictors, and a corresponding accuracy of 85.0% (kappa of 0.45) was achieved when an existing soil map was used as well. In addition to the topography-related features, soil stoniness proved to be a critical factor affecting soil resistance to rutting. Our results indicate that the utilisation of LiDAR-derived elevation data for terrain trafficability mapping is a feasible method in sustainable forest management. 相似文献
159.
Jiri Pyörälä Ville Kankare Mikko Vastaranta Juha Rikala Markus Holopainen Marketta Sipi 《Scandinavian Journal of Forest Research》2018,33(3):291-298
While X-ray scanning is increasingly used to measure the interior quality of logs, terrestrial laser scanning (TLS) could be used to collect information on external tree characteristics. As branches are one key indicator of wood quality, we compared TLS and X-ray scanning data in deriving whorl locations and each whorl’s maximum branch and knot diameters for 162 Scots pine (Pinus sylvestris L.) log sections. The mean number of identified whorls per tree was 37.25 and 22.93 using X-ray and TLS data, respectively. The lowest TLS-derived whorl in each sample tree was an average 5.56?m higher than that of the X-ray data. Whorl-to-whorl mean distances and the means of the maximum branch and knot diameters in a whorl measured for each sample tree using TLS and X-ray data had mean differences of ?0.12?m and ?6.5?mm, respectively. One of the most utilized wood quality indicators, tree-specific maximum knot diameter measured by X-ray, had no statistically significant difference to the tree-specific maximum branch diameter measured from the TLS point cloud. It appears challenging to directly derive comparative branch structure information using TLS and X-ray. However, some features that are extractable from TLS point clouds are potential wood quality indicators. 相似文献
160.
Maxim E. Zhidkov Moritz Kaune Alexey V. Kantemirov Polina A. Smirnova Pavel V. Spirin Maria A. Sidorova Sergey A. Stadnik Elena Y. Shyrokova Dmitry N. Kaluzhny Oleg A. Tryapkin Tobias Busenbender Jessica Hauschild Tina Rohlfing Vladimir S. Prassolov Carsten Bokemeyer Markus Graefen Gunhild von Amsberg Sergey A. Dyshlovoy 《Marine drugs》2022,20(3)
Marine alkaloid fascaplysin and its derivatives are known to exhibit promising anticancer properties in vitro and in vivo. However, toxicity of these molecules to non-cancer cells was identified as a main limitation for their clinical use. Here, for the very first time, we synthesized a library of fascaplysin derivatives covering all possible substituent introduction sites, i.e., cycles A, C and E of the 12H-pyrido[1-2-a:3,4-b’]diindole system. Their selectivity towards human prostate cancer versus non-cancer cells, as well as the effects on cellular metabolism, membrane integrity, cell cycle progression, apoptosis induction and their ability to intercalate into DNA were investigated. A pronounced selectivity for cancer cells was observed for the family of di- and trisubstituted halogen derivatives (modification of cycles A and E), while a modification of cycle C resulted in a stronger activity in therapy-resistant PC-3 cells. Among others, 3,10-dibromofascaplysin exhibited the highest selectivity, presumably due to the cytostatic effects executed via the targeting of cellular metabolism. Moreover, an introduction of radical substituents at C-9, C-10 or C-10 plus C-3 resulted in a notable reduction in DNA intercalating activity and improved selectivity. Taken together, our research contributes to understanding the structure–activity relationships of fascaplysin alkaloids and defines further directions of the structural optimization. 相似文献