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131.
Growth of subarctic Scots pine (Pinus sylvestris L.) trees was investigated by a combination of process-based models and dendroecological approaches. Tree ring width indices were strongly autocorrelated and correlated with simulated photosynthetic production of the previous year and with organic matter N mineralization of the current year. An autoregressive model, with photosynthesis and N mineralization as external inputs, explained growth of the trees well. However, relationships for the period 1950-1992 differed significantly from relationships for the period 1876-1949; the slope of the regression of tree ring width index and photosynthesis was lower for the 1950-1992 period. Also, the autocorrelation structure of the data changed. First-order autocorrelation decreased and second-order autocorrelation increased from the earlier to the later period. This means that growth is becoming less sensitive to variations in photosynthetic production, whereas the relationships between growth and N mineralization are remaining fairly constant. We postulate that, although photosynthesis has increased in response to increasing CO2 concentrations, tree growth rate cannot parallel the increase in photosynthesis because potential growth rate is limited directly by temperature.  相似文献   
132.
Despite the steadily increasing area under protected agriculture there is a current lack of knowledge about the effects of the 30% black shading screen on microclimate and crop water requirements. Meteorological and lysimeter measurements inside a screenhouse planted with sweet pepper were compared to external reference data. Irrigation water use efficiency (IWUE) was calculated from yield records Y and water meter readings I applied. Shading reduced mean global radiation R G by more than 40%, and the screen transmissivity τ screen was shown to vary with solar elevation angle β. Wind speed inside the screenhouse u in was reduced by more than 50%. Crop water requirements ET c were 38% lower than estimates for an open field crop, suggesting a significant water saving potential when using screenhouses. However, the screen did not significantly modify maximum temperature T max and daily vapor pressure deficit. The FAO-Penman–Monteith approach based on meteorological measurements in the screenhouse accurately predicted daily crop evapotranspiration, and was in close agreement with lysimeter measurements. IWUE was relatively high (10.7 kg m−3 in 2004 and 13.5 kg m−3 in 2005), but additional research is required to quantify the effect of shading on yield as well as to determine the water saving potential of other commonly used screens. Contribution no. 603/06 from the Agricultural Research Organization An erratum to this article can be found at  相似文献   
133.
The AcrA/AcrB/TolC complex spans the inner and outer membranes of Escherichia coli and serves as its major drug-resistance pump. Driven by the proton motive force, it mediates the efflux of bile salts, detergents, organic solvents, and many structurally unrelated antibiotics. Here, we report a crystallographic structure of trimeric AcrB determined at 2.9 and 3.0 angstrom resolution in space groups that allow asymmetry of the monomers. This structure reveals three different monomer conformations representing consecutive states in a transport cycle. The structural data imply an alternating access mechanism and a novel peristaltic mode of drug transport by this type of transporter.  相似文献   
134.
135.
Tropospheric ozone and black carbon (BC) contribute to both degraded air quality and global warming. We considered ~400 emission control measures to reduce these pollutants by using current technology and experience. We identified 14 measures targeting methane and BC emissions that reduce projected global mean warming ~0.5°C by 2050. This strategy avoids 0.7 to 4.7 million annual premature deaths from outdoor air pollution and increases annual crop yields by 30 to 135 million metric tons due to ozone reductions in 2030 and beyond. Benefits of methane emissions reductions are valued at $700 to $5000 per metric ton, which is well above typical marginal abatement costs (less than $250). The selected controls target different sources and influence climate on shorter time scales than those of carbon dioxide-reduction measures. Implementing both substantially reduces the risks of crossing the 2°C threshold.  相似文献   
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137.
Excessive nutrient loading is a major threat to aquatic ecosystems worldwide that leads to profound changes in aquatic biodiversity and biogeochemical processes. Systematic quantitative assessment of functional ecosystem measures for river networks is, however, lacking, especially at continental scales. Here, we narrow this gap by means of a pan-European field experiment on a fundamental ecosystem process--leaf-litter breakdown--in 100 streams across a greater than 1000-fold nutrient gradient. Dramatically slowed breakdown at both extremes of the gradient indicated strong nutrient limitation in unaffected systems, potential for strong stimulation in moderately altered systems, and inhibition in highly polluted streams. This large-scale response pattern emphasizes the need to complement established structural approaches (such as water chemistry, hydrogeomorphology, and biological diversity metrics) with functional measures (such as litter-breakdown rate, whole-system metabolism, and nutrient spiraling) for assessing ecosystem health.  相似文献   
138.
Entanglement is the essential feature of quantum mechanics. Notably, observers of two or more entangled particles will find correlations in their measurement results that cannot be explained by classical statistics. To make it a useful resource, particularly for scalable long-distance quantum communication, the heralded generation of entanglement between distant massive quantum systems is necessary. We report on the creation and analysis of heralded entanglement between spins of two single rubidium-87 atoms trapped independently 20 meters apart. Our results illustrate the viability of an integral resource for quantum information science, as well as for fundamental tests of quantum mechanics.  相似文献   
139.
The use of living mulch (LM) systems for maize (Zea mays L.) cultivation may reduce soil erosion and nitrate leaching. However, the yield of maize cultivated in LM is lower than in conventional farming systems. This decrease has often been attributed to belowground competition, but the lack of a suitable method to demonstrate such competition has prevented further investigation. A recently developed method allows for the direct and non-destructive observation of root growth in LM. Maize expressing a green fluorescence protein was grown in monoculture or together with Italian ryegrass (Lolium multiflorum Lam.). Root growth was screened in minirhizotrons from the time of sowing to the anthesis of the maize.Compared with the maize cultivated without Italian ryegrass, the cultivation in the LM significantly (p < 0.05) reduced the shoot dry matter (56%), leaf area (39%) and mean root density (41%) of the maize at anthesis. An analysis of variance showed that the reduction due to the living Italian ryegrass in the root density of the maize was not significantly different from the reduction in leaf area. Similarly, the reduction in the root density and shoot dry matter of the maize were not significantly different. The roots of the LM maize grew in the soil where the LM was removed but also where the LM continued growing. This study constitutes the first direct quantification of root growth and distribution in a LM system.  相似文献   
140.
Bluetongue (BT) is an infectious, non-contagious disease of wild and domestic ruminants. It is caused by bluetongue virus (BTV) and transmitted by Culicoides biting midges. Since 1998, BT has been emerging throughout Europe, threatening not only the na?ve ruminant population. Historically, South American camelids (SAC) were considered to be resistant to BT disease. However, recent fatalities related to BTV in captive SAC have raised questions about their role in BTV epidemiology. Data on the susceptibility of SAC to experimental infection with BTV serotype 8 (BTV-8) were collected in an animal experiment. Three alpacas (Vicugna pacos) and three llamas (Lama glama) were experimentally infected with BTV-8. They displayed very mild clinical signs. Seroconversion was first measured 6-8 days after infection (dpi) by ELISA, and neutralising antibodies appeared 10-13 dpi. BTV-8 RNA levels in blood were very low, and quickly cleared after seroconversion. However, spleens collected post-mortem were still positive for BTV RNA, over 71 days after the last detection in blood samples. Virus isolation was only possible from blood samples of two alpacas by inoculation of highly sensitive interferon alpha/beta receptor-deficient (IFNAR(-/-)) mice. An in vitro experiment demonstrated that significantly lower amounts of BTV-8 adsorb to SAC blood cells than to bovine blood cells. Although this experiment showed that SAC are generally susceptible to a BTV-8 infection, it indicates that these species play a negligible role in BTV epidemiology.  相似文献   
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