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排序方式: 共有726条查询结果,搜索用时 15 毫秒
71.
Rmi Cousin Hugo Groult Chanez Manseur Romain Ferru-Clment Mario Gani Rachel Havret Claire Toucheteau Grgoire Prunier Batrice Colin Franck Morel Jean-Marie Piot Isabelle Lanneluc Kvin Baranger Thierry Maugard Ingrid Fruitier-Arnaudin 《Marine drugs》2021,19(10)
Sugar-based molecules such as heparins or natural heparan sulfate polysaccharides have been developed and widely studied for controlling heparanase (HPSE) enzymatic activity, a key player in extracellular matrix remodelling during cancer pathogenesis. However, non-enzymatic functions of HPSE have also been described in tumour mechanisms. Given their versatile properties, we hypothesized that sugar-based inhibitors may interfere with enzymatic but also non-enzymatic HPSE activities. In this work, we assessed the effects of an original marine λ-carrageenan derived oligosaccharide (λ-CO) we previously described, along with those of its native counterpart and heparins, on cell viability, proliferation, migration, and invasion of MDA-MB-231 breast cancer cells but also of sh-MDA-MB-231 cells, in which the expression of HPSE was selectively downregulated. We observed no cytotoxic and no anti-proliferative effects of our compounds but surprisingly λ-CO was the most efficient to reduce cell migration and invasion compared with heparins, and in a HPSE-dependent manner. We provided evidence that λ-CO tightly controlled a HPSE/MMP-14/MMP-2 axis, leading to reduced MMP-2 activity. Altogether, this study highlights λ-CO as a potent HPSE “modulator” capable of reducing not only the enzymatic activity of HPSE but also the functions controlled by the HPSE levels. 相似文献
72.
Ioannis E. Papadakis Mario M. Zaiss Yiannos Kyriakou Georgios Georgiou Pascal Divanach Constantinos C. Mylonas 《Aquaculture (Amsterdam, Netherlands)》2009,286(1-2):45-52
The influence of the absence of Artemia nauplii from larval diet protocols on growth and digestive system ontogeny was studied using histological techniques in the shi drum (Umbrina cirrosa). One group of larvae was reared using the standard intensive rearing protocol, which offers a combination of enriched rotifers (Brachionus plicatilis), Artemia spp. nauplii and artificial diet (Std-group). Another group was reared using the same protocol, but without the offering of Artemia nauplii (group No-Artemia). The ontogenesis of the digestive system from hatching to metamorphosis was a very rapid process, and there were no differences between the two feeding regimes in the temporal appearance of the various components of the digestive system. The first organised presence of the hepatic and pancreatic tissue appeared at 2–3 d after hatching (dah), suggesting that these organs function from a very early developmental stage. In the No-Artemia larvae between 13 and 29 dah there was a reduction in the height of enterocytes in the intestinal mucosa, a progressive flattening of the primary intestinal folds in the anterior and posterior intestine and a decrease in lipid stores in the liver, suggesting a period of relative starvation. However, by the end of the study at 41 dah, there were no significant differences in body length, intestinal morphology or liver lipid stores between larvae reared under the two feeding regimes. The study suggests that the diet may influence the maturation and/or function, but not the ontogeny of the digestive system. Furthermore, the rapid differentiation of the digestive system in shi drum and the prompt recovery of the No-Artemia larvae from the symptoms of starvation by 29 dah, indicate a plasticity during ontogenesis and the ability of larvae to adapt to artificial diets at very early developmental stages. 相似文献
73.
Gaëlle Roudaut Mario Maglione Dagmar van Dusschoten Martine Le Meste 《Cereal Chemistry》1999,76(1):70-77
The molecular mobility in low-moisture (<9%, web) white bread was studied as a function of temperature using pulsed-proton nuclear magnetic resonance (NMR), and dielectric and dynamic mechanical spectroscopies. The water was mobile, even in glassy samples. Different processes below glass transition temperature (sub-Tg) were observed, and a relaxation map of the studied system was drawn. These results have been interpreted and extrapolated to suggest that the Tg is not a universal predictive parameter for the physical stability of glassy food. 相似文献
74.
Elisabete Yurie Sataque Ono Elaine Cunha Moreno Mario Augusto Ono Carolina Nachi Rossi Gervásio Hitoshi Saito Édio Vizoni Yoshitsugu Sugiura Elisa Yoko Hirooka 《European journal of plant pathology / European Foundation for Plant Pathology》2011,131(4):653-660
In this study the effect of different cropping systems and crop successions was evaluated on natural Fusarium sp. contamination and fumonisin levels in corn. The cropping systems consisted of a conventional and no-tillage area cultivated
with corn in the summer following either oats or fallow in the winter (2006 and 2007 growing seasons). In addition, the effect
of applying nitrogen fertilizer (0, 22.5, 45.0, 90.0 and 90.0 kg ha−1 nitrogen supplemented with potassium oxide) on fumonisin contamination was evaluated in the 2006 growing season. Fusarium sp. was detected in 90% samples in 2006 and in 100% samples in 2007. In both growing seasons, no-till corn following oats
showed the highest mean fumonisin levels and differed significantly (P < 0.05) from all the others (2006) and from conventional till corn following either oats or fallow in the winter (2007).
Fumonisin levels ranged from 0.13 to 19.52 μg g−1 (mean 6.97 μg g−1) and from 3.70 to 7.75 μg g−1 (mean 6.29 μg g−1) in no-till corn following oats from the 2006 and 2007 growing seasons, respectively. Plots treated with 0 kg ha−1 and 22.5 kg ha−1 nitrogen showed the highest mean fumonisin levels and differed significantly from those with 45.0 and 90 kg ha−1 nitrogen. Fumonisin levels correlated negatively (P < 0.05) with the nitrogen fertilization rates. Although no-till is advantageous from a soil conservation standpoint, it may
enhance the potential for fumonisin contamination in corn. 相似文献
75.
Hiroto Miura Takuya Hashimoto Yukiko Kawanishi Hiroki Kawauchi Ryo Inoue Noriaki Shoji Kunihiko Saito Mario Sekiya Yosuke Saito Jumpei Yasuda Chiemi Yonezawa Tetsushiro Endo Hirotaka Kasuya Yutaka Suzuki Yasuo Kobayashi Satoshi Koike 《Animal Science Journal》2021,92(1):e13601
The rumen microbiota comprises a vast range of bacterial taxa, which may affect the production of high-quality meat in Japanese Black cattle. The aim of this study was to identify core rumen microbiota in rumen fluid samples collected from 74 Japanese Black cattle raised under different dietary conditions using 16S rRNA gene amplicon sequencing. In the rumen of fattening Japanese Black cattle, 10 bacterial taxa, showing >1% average relative abundance and >95% prevalence, irrespective of the dietary conditions and the fattening periods, were identified as the core rumen bacterial taxa, which accounted for approximately 80% of the rumen microbiota in Japanese Black cattle. Additionally, population dynamics of the core rumen bacterial taxa revealed two distinct patterns: Prevotella spp. and unclassified Bacteroidales decreased in the mid-fattening period, whereas unclassified Clostridiales, unclassified Ruminococcaceae, Ruminococcus spp., and unclassified Christensenellaceae increased during the same period. Therefore, the present study reports the wide distribution of the core rumen bacterial taxa in Japanese Black cattle, and the complementary nature of the population dynamics of these core taxa, which may ensure stable rumen fermentation during the fattening period. 相似文献
76.
Early life history connectivity of Antarctic silverfish (Pleuragramma antarctica) in the Ross Sea 下载免费PDF全文
Cassandra M. Brooks Jilda Alicia Caccavo Julian Ashford Robert Dunbar Kimberly Goetz Mario La Mesa Lorenzo Zane 《Fisheries Oceanography》2018,27(3):274-287
A recent population hypothesis for Antarctic silverfish (Pleuragramma antarctica), a critical forage species, argued that interactions between life history and circulation associated with glacial trough systems drive circumpolar distributions over the continental shelf. In the Ross Sea, aggregations of eggs and larvae occur under fast ice in Terra Nova Bay, and the hypothesis predicted that dispersing larvae encounter outflow along the western side of Drygalski Trough. The outflow advects larvae towards the shelf‐break, and mixing with trough inflow facilitates return toward the inner shelf. To examine the hypothesis, we compared samples of P. antarctica collected near Coulman Island in the outflow, along Crary Bank in the inflow, and a third set taken over the rest of the Ross Sea. We ruled out misidentification using an innovative genetic validation. Silverfish larvae comprised 99.5% of the catch, and the highest population densities were found in Drygalski Trough. The results provided no evidence to reject the population hypothesis. Abundance indices, back‐calculated hatching dates, length distributions and growth were congruent with a unified early life history in the western Ross Sea, constrained by cryopelagic early stages in Terra Nova Bay. By contrast, a sample in the Bay of Whales revealed much smaller larvae, suggesting either a geographically separate population in the eastern Ross Sea, or westward connectivity with larvae spawned nearby by fish sourced from troughs upstream in the Amundsen Sea. These results illustrate how hypotheses that integrate population structure with life history can provide precise spatial predictions for subsequent testing. 相似文献
77.
Schirra M Palma A D'Aquino S Angioni A Minello EV Melis M Cabras P 《Journal of agricultural and food chemistry》2008,56(2):455-460
The present study investigated the influence of a hot water dip (HWD) for 2 min at 50 degrees C, a standard and effective treatment for postharvest decay control of citrus fruit, on the nutritional and health-related properties of kumquats. The results show that most of the parameters examined, including titratable acidity, soluble solids content, maturity index, glucose, fructose, sucrose, ascorbic acid, dehydroascorbic acid, alpha- and gamma-tocopherols, beta-carotene, zeaxantin, rhoifolin, and antioxidant activity, were not significantly affected by treatment. The levels of beta-cryptoxanthin, narirutin, and total flavonoids increased after HWD, whereas lutein and total phenols decreased. The concentration of the essential oil and the relative percentage of the individual components of the essential oil were not affected by HWD except for the minor compound p-menta-1,5-dien-1-ol, which increased after HWD. After storage, lower levels of glucose, total sugars, beta-carotene, beta-cryptoxanthin and lutein were recorded in HWD fruit. A decrease in antioxidant activity and increases in alpha-tocopherol and total vitamin E were found both in control and HWD fruit. The influence of HWD at 50 degrees C for 2 min on individual nutraceuticals and health-related properties was thus generally low and may depend on storage conditions. 相似文献
78.
Giorgi M Cuniberti B Ye G Barbero R Sgorbini M Vercelli C Corazza M Re G 《Veterinary journal (London, England : 1997)》2011,190(1):143-149
Tepoxalin is a non-steroidal anti-inflammatory drug with analgesic, anti-inflammatory, and antipyretic properties and has been recently introduced into veterinary medicine. The aim of this study was to evaluate the pharmacokinetic/pharmacodynamic (PK/PD) profile of tepoxalin to assess whether it would be suitable for clinical use in horses. Six female fasting/fed horses were given 10mg/kg tepoxalin orally in a cross-over study. After administration, tepoxalin underwent rapid and extensive hydrolytic conversion to its carboxylic acid metabolite RWJ-20142. In animals that had been fed, the plasma concentrations of tepoxalin were undetectable, whereas in fasting animals they were close to the limit of quantification of the method. No differences between the fasting/fed groups in RWJ-20142 plasma concentrations were shown. Tepoxalin showed a strong and long-lasting ex vivo inhibitory activity against cyclooxygenase (COX)-1, mainly due to its main metabolite RWJ-20142. Tepoxalin and RWJ-20142 do not seem to possess either COX-2 or 5-lipoxygenase inhibitory activity in the horse. These features suggest that the drug is a selective COX-1 inhibitor in horses, with no significant anti-inflammatory activity. Thus, its long term use in equine practice could be of concern. 相似文献
79.
Mario A. Burgos-Aceves Rafael Campos-Ramos Danitzia A. Guerrero-Tortolero 《Fish physiology and biochemistry》2010,36(4):1263-1269
The peripheral blood cells and differential blood profile of captive female and male leopard grouper Mycteroperca
rosacea are described for aquacultural purposes. Basophilic, polychromatic, and orthochromic erythroblasts were observed as immature
erythrocytes that develop into mature erythrocytes. Young erythrocytes were not evident, and bi-lobed erythrocytes were extremely
scarce. Types of leukocytes included lymphocytes; three types of granulocytes (basophiles, eosinophiles, and neutrophiles);
monocytes; and a specialized amoeboid-like macrophage in the blood, which has not been previously described in fish-blood
literature. Thrombocytes were commonly observed. There was significantly higher erythropoiesis in males. Granulocytes and
lymphocytes of females were significantly higher than males, whereas monocytes and thrombocytes were not. 相似文献
80.
Mario Augusto Pagnotta Linda Mondini Pasquale Codianni Clara Fares 《Genetic Resources and Crop Evolution》2009,56(3):299-310
Emmer wheat (Triticum dicoccon Schrank, 2n = 4x = 28) consists in a hulled wheat; its cultivation has been drastically reduced during the last century as a consequence of
its low yield. Recently, its agronomic and nutritive values, as well as the increase of popularity of organic agriculture,
have led to a renewed interest making its cultivation economically viable in the marginal lands with an increase of the cultivated
areas. In Italy, it mainly survives in few marginal lands of central and southern Italy, where local varieties, adapted to
the natural environment from where they originate, are used; moreover, some selected lines have also been developed. In the
present work, agro-morphological and qualitative traits, together with molecular analyses of 20 emmer accessions consisting
of Italian landraces, breeding lines, and cultivars, were performed. The field experiments were conducted for two consecutive
years (2001/2002–2002/2003) in two locations: Viterbo in central Italy, and Foggia in south Italy. The analyzed emmer wheat
accessions showed a good amount of genetic variability for both evaluated agro-morphological and molecular traits. This study
illustrates an increase in earliness, GY, TW, TKW, and YI going from landraces, breeding lines to cultivars, while the variability
does not change proportionally. 相似文献