Vasovagal tonus index (VVTI) as an indirect assessment of remission status in canine multicentric lymphoma undergoing multi-drug chemotherapy

Vasovagal tonus index (VVTI) is an indirect measure of heart rate variability and may serve as a marker of disease severity. Higher heart rate variability has predicted lower tumour burden and improved survival in humans with various tumour types. The purpose of this pilot study was to evaluate VVTI as a biomarker of remission status in canine lymphoma. The primary hypothesis was that VVTI would be increased in dogs in remission compared to dogs out of remission. Twenty-seven dogs were prospectively enrolled if they had a diagnosis of intermediate to high-grade lymphoma and underwent multidrug chemotherapy. Serial electrocardiogram data were collected under standard conditions and relationships between VVTI, remission status and other clinical variables were evaluated. VVTI from dogs in remission (partial or complete) did not differ from dogs with fulminant lymphoma (naive or at time of relapse). Dogs in partial remission had higher VVTI than dogs in complete remission (p = 0.021). Higher baseline VVTI was associated with higher subsequent scores (p < 0.001). VVTI also correlated with anxiety level (p = 0.03). Based on this pilot study, VVTI did not hold any obvious promise as a useful clinical biomarker of remission status. Further investigation may better elucidate the clinical and prognostic utility of VVTI in dogs with lymphoma.     

Application of peracetic acid and quarternary ammonium disinfectants as a part of sanitary treatment in a poultry house and poultry processing plant

Hygiene and sanitation play a major role in any effective disease control programme for poultry production and processing premises. Various deficiencies in disinfection may induce that chains of infections are not broken from one stock to another. The present study investigated the efficacy of disinfection on a broiler farm and in a plant processing poultry from the investigated farm. Besides inspection of disinfection, the influence of contamination on broiler carcasses and consequences of this contamination on sanitation of the processing lines were studied. Swabs from surfaces coming into contact with the handled raw material were taken and evaluated. The results obtained by a standard microbiological swab method were evaluated and compared with an ATP-bioluminescence method. The investigations included determination of total counts of microorganisms, coliform bacteria and moulds. When employing the standard plate-count method, the total counts of microorganisms (TCM) reached <1, 1-100 and >100 CFU in 0, 12 and 88% swabs on poultry farm and in 22%, 36% and 42% swabs in the processing plant, respectively. The bioluminescence method was used only in the processing plant and contamination corresponding to <100, 100-300 and >300 relative light units (RLU) was detected in 80%, 10% and 10% swabs respectively.     

Integrated approach for improving small scale market oriented dairy systems in Pakistan: participatory rural appraisal and economic opportunity survey

Livestock production is an integral part of the rain-fed and irrigated agriculture system in Pakistan. Animal production is closely interlinked with the cropping systems and play a crucial role in the rural economy. Participatory rural appraisals and economic opportunity surveys were conducted in two ecological zones (irrigated and rain-fed) and two dairy production systems (peri-urban and mixed livestock). Major constraints in animal health, nutrition and reproduction were identified and interventions were suggested to overcome these difficulties. The economic opportunity survey revealed that maximum opportunity to enhance farmers’ income is to increase milk production per day per animal, which can be accomplished through coordinated improvements in nutrition, reproduction and genetics.     

Effect of supplementation of mustard oil cake on intake,digestibility and microbial protein synthesis of cattle in a straw-based diet in Bangladesh

The objective of this study was to analyse the effects of different levels of rumen-degradable protein (RDP) on intake, digestibility and microbial protein synthesis by supplementing mustard oil cake (MOC) on rice straw-based diet of cattle (Bos indicus) in Bangladesh. A 4 × 4 Latin square design was applied. Four diets having constant energy (7.0 MJ/kg of dry matter (DM)) with varying levels of RDP (M ₀ = 4.1 g/MJ (control), M ₁ = 6.3 g/MJ, M ₂ = 8.3 g/MJ and M ₃ = 12.4 g/MJ of metabolizable energy (ME)) were received by each animal for a period of 28 days. A metabolism trial was conducted for 7 days. Results indicate that with increasing levels of RDP, crude protein (CP) and RDP intake increased significantly (P < 0.01). The significant (P < 0.01) increase in digestibility values are obtained for DM, organic matter, CP and digestible organic matter in the rumen. The digestibility of neutral detergent fibre and acid detergent fibre was also increased significantly (P < 0.05). The total nitrogen (N), ammonia-N and total volatile fatty acids increase significantly (P < 0.01) while the rumen pH increased from M ₀ to M ₂ and decreased thereafter. The efficiency microbial N intake increased significantly (P < 0.01) but showed a curvilinear response with higher RDP level (12.40 g/RDP/MJ ME). This study concludes that supplementation of RDP from MOC enhances the intake, digestibility and microbial protein synthesis which ultimately increases utilization of low-quality feed resources that can be used for developing cost-effective feeding systems on a straw-based diet in tropical regions.     

Characterization of indigenous chicken production systems in Kenya

Indigenous chicken (IC) and their production systems were characterized to understand how the whole system operates for purposes of identifying threats and opportunities for holistic improvement. A survey involving 594 households was conducted in six counties with the highest population of IC in Kenya using structured questionnaires. Data on IC farmers’ management practices were collected and analysed and inbreeding levels calculated based on the effective population size. Indigenous chicken were ranked highest as a source of livestock income by households in medium- to high-potential agricultural areas, but trailed goats in arid and semi-arid areas. The production system practised was mainly low-input and small-scale free range, with mean flock size of 22.40 chickens per household. The mean effective population size was 16.02, translating to high levels of inbreeding (3.12%). Provision for food and cash income were the main reasons for raising IC, whilst high mortality due to diseases, poor nutrition, housing and marketing channels were the major constraints faced by farmers. Management strategies targeting improved healthcare, nutrition and housing require urgent mitigation measures, whilst rural access road network needs to be developed for ease of market accessibility. Sustainable genetic improvement programmes that account for farmers’ multiple objectives, market requirements and the production circumstances should be developed for a full realization of IC productivity.     

Prevalence and antimicrobial resistance profiles of <Emphasis Type="Italic">Salmonella enterica</Emphasis> serovars isolated from slaughtered cattle in Bahir Dar,Ethiopia

A study was undertaken from October 2006 to March 2007 to determine the prevalence and antimicrobial resistance patterns of Salmonella serovars. Liver, mesenteric lymph nodes, intestinal content, and carcass swab samples (each n?=?186) were collected from 186 apparently healthy slaughtered cattle at Bahir Dar abattoir. Bacteriological analysis was done according to the International Organization for Standardization (ISO 6579 2002). Isolates were serotyped at Agence Française de Securite Sanitaire des Aliments, Cedex, France. Twenty-eight isolates consisting of Salmonella Typhimurium, Salmonella Newport, Salmonella Haifa, Salmonella Heidelberg, Salmonella Infantis, and Salmonella Mishmarhaemek were identified. Salmonella Typhimurium and Salmonella Newport were most frequently isolated while Salmonella Heidelberg and Salmonella Mishmarhaemek were isolated least. Eleven of the 28 (39.3%) were resistant to one or more of the antimicrobials tested. Resistance was shown to ampicillin, chloramphenicol, gentamycin, norfloxacin, polymyxin-B, streptomycin, tetracycline, and trimethoprim. Four of 11 (36.4%) were multiple antimicrobial resistant. All the isolates tested were susceptible to the antimicrobial effects of gentamycin, norfloxacin, and trimethoprim. Eleven, four, and two isolates of the 28 were resistant to streptomycin, tetracycline, and ampicillin, respectively. All isolates of Salmonella Infantis, Salmonella Typhimurium (except one), and Salmonella Mishmarhaemek were susceptible to the tested antimicrobials. One Typhimurium isolate was resistant to chloramphenicol, streptomycin, and tetracycline. Salmonella Haifa was multiply antimicrobial resistant to ampicillin, tetracycline, and streptomycin. All isolates of Salmonella Heidelberg were resistant to streptomycin. Results of this study indicated high level of carcass contamination with antimicrobial-resistant Salmonella serovars which could pose public health risk; suggests need for hygienic slaughtering operations and proper cooking of meat before consumption. Further detailed studies involving different abattoirs, animal products, food items, and animals on different settings were recommended in the study area.     

Responses to graded replacement of urea by maize steep liquor in diets for intensively fed lambs for meat production

Urea is a common ingredient of the diets of intensively fed lambs, but is increasingly required for industrial processes. Maize steep liquor (MSL) is a by-product of maize grain degradation to produce starch that may be a suitable replacement. Fifty growing lambs were fed on equinitrogenous diets in which between 0% and 80% of the urea was replaced by MSL; their growth and metabolism were recorded over 70 days. Increasing replacement of urea by MSL increased feed intake and nutrient digestibilities, leading to increased growth rates, more efficient feed conversion, and increased nitrogen retention. Concentrations of triiodothyroxin, thyroxin, glucose, and methionine were increased by replacement of urea by liquor, and plasma urea was reduced. This study suggests that MSL is a suitable replacement for up to 80% of urea in the diet of rapidly growing lambs.     

Rumen development and growth of Balouchi lambs offered alfalfa hay pre- and post-weaning

The consumption of solid feed is essential for successful transition from a pre-ruminant to a functional digestive tract. Lambs fed starter rations containing highly fermentable carbohydrates often experience dramatic changes in concentrations of rumen and blood metabolites. The optimal amount of roughage required in the diet of pre-ruminant animals is still unclear. The objective of this study was to determine the effect of feeding alfalfa hay on performance and rumen development in young Balouchi lambs. In a completely randomized design, 30 lambs were fed one of three experimental diets consisting of a control, without alfalfa hay (C), a diet containing 7.5% alfalfa hay (A1), and a diet containing 15% alfalfa hay (A2). Lambs fed A1 and A2 diets had lower dry matter intake during the pre-weaning period (P?P?=?0.02), but feed conversion ratio and average daily gain were not affected by feeding alfalfa hay. Concentration of beta-hydroxybutyric acid was higher in C compared with the A1 and A2 groups (P?P?=?0.04) and increased thickness of muscular layer (P?=?0.05). We concluded that including 15% alfalfa hay in the starter diet could reduce thickness of the keratinization layer and increase muscularity of rumen wall without adverse effects on growth and performance of newborn lambs.     

Improvement in the diagnosis of <Emphasis Type="Italic">Brucella abortus</Emphasis> infections in naturally infected water buffaloes (<Emphasis Type="Italic">Bubalus bubalis</Emphasis>) using an ELISA with a Protein-G-based indicator system

Brucellosis caused by Brucella abortus in domestic water buffaloes (Bubalus bubalis) raised under the traditional system of husbandry in northern India was diagnosed using an enzyme-linked immunosorbent assays (ELISA) with a Protein-G-based indicator system (Protein-G ELISA). A total of 1,551 animals that are positive (N = 61), negative (N = 243), and suspected (N = 1,247) for brucellosis were examined. Rose bengal test (RBT) was used to predict the disease, and accordingly, animals were dichotomized in positive and negative population for receiver operating characteristic (ROC) curve analysis to determine the sensitivity, the specificity, and the performance index of Protein-G ELISA. Taking all animals (N=1551) into account, the ROC curve analysis revealed cut off value of 29.6% positivity (%P) with 98.40% and 94.94%, sensitivity and specificity, respectively. The results were compared with ELISA in which anti-bovine conjugate was used. The cut off in ELISA was 37.9%P and sensitivity and specificity were 96.26% and 97.07%, respectively. The performance indexes of both the assays were almost equal and were 193.34 for Protein-G ELISA and 193.33 for ELISA. The cut off values of both the tests changed, if only known positive (N = 61) and known negative (N=243) animals were used for ROC curve analysis, and accordingly, changes in sensitivity and specificity were observed with significant decrease of performance indexes of both the tests. The high optical density (P<0.0001) background signal with negative serum control and high %P (P<0.0001) in sera from negative population were noticed in ELISA in comparison to Protein-G ELISA.     

In vitro inhibitory effect of trichostatin A on canine grade 3 mast cell tumor

Mast cell tumor (MCT) is one of the most prevalent neoplasms that affect skin and soft tissue in dogs. Because mast cell tumors present a great variety of clinical appearance and behavior, their treatment becomes a challenge. Trichostatin A (TSA), an antifungal antibiotic, has shown inhibitory effects on the proliferation and induction of apoptosis in various types of cancer cells. In order to evaluate the potential of trichostatin A as a therapeutic drug, cells of grade 3 MCT were cultured and treated with concentrations of 1 nM to 400 nM of TSA. MTT assay and trypan blue exclusion assays were performed to estimate cell growth and cell viability, and cell cycle analysis was evaluated. TSA treatment showed a reduction in numbers of viable cells and an increase of cell death by apoptosis. The cell cycle analysis showed an increase of hypodiploid cells and a reduction of G0/G1 and G2/M –phases. According to these results, trichostatin A may be an interesting potential chemotherapeutic agent for the treatment of canine MCT.