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Alessandro Vetere Mara Bertocchi Igor Pelizzone Giuseppe F. La Cauza Laura Denti Enrico Bigliardi Francesco Di Ianni 《Journal of veterinary diagnostic investigation》2021,33(1):116
Forty-five Horsfield’s tortoises (Testudo horsfieldii; syn. Agrionemys horfieldii, Russian tortoise) belonging to different owners had decreased appetite and respiratory issues. Twenty-nine tortoises had epiphora, dyspnea, and white necrotic diphtheroid oral plaques (group G1). Ten of the remaining 16 tortoises had serious dehydration, appetite disorder, and depression (G2). The last 6 tortoises had only decreased appetite and moderate conjunctival discharge (G3). During the physical examination of all 45 tortoises, a cytologic sample and an oral swab for herpesvirus and Mycoplasma agassizii PCR testing were taken. In 20 of 29 specimens from G1, in 8 of 16 from G2, and 0 of 6 from G3, the cytologic exam revealed intranuclear acidophilic inclusion bodies, multinucleate cellular syncytia, and further abnormalities caused by herpesviral infection. Moreover, all 45 tested subjects were found to be positive for testudinid herpesvirus 1; 2 were positive for M. agassizii. This prospective study suggests that Horsfield’s tortoises with such signs would benefit from this screening procedure, given that it was effective in a significant proportion of infected and symptomatic animals, and no negative effects were seen. 相似文献
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Risely Ferraz De Almeida Camila Haddad Silveira Raquel P. Mota Mara Moitinho Everton Martins Arruda Eduardo De Sá Mendonça Newton La Scala Beno Wendling 《Journal of Soils and Sediments》2016,16(10):2354-2364
Purpose
The mineralization/immobilization of nutrients from the crop residues is correlated with the quality of the plant material and carbon compartments in the recalcitrant and labile soil fractions. The objective of this study was to correlate the quality and quantity of crop residues incubated in the soil with carbon compartments and CO2-C emission, using multivariate analysis.Materials and methods
The experiment was conducted in factorial 4?+?2?+?5 with three replicates, referring to three types of residues (control, sugarcane, Brachiaria, and soybean), and two contributions of the crop residues in constant rate, CR (10 Mg ha?1 residue), and agronomic rate, AR (20, 8, and 5 Mg ha?1 residue, respectively, for sugarcane, soybean, and Brachiaria), evaluated five times (1, 3, 6, 12, and 48 days after incubation). At each time, we determined the CO2-C emission, nitrogen and organic carbon in the soil, and the residues. In addition, the microbial biomass and water-soluble, labile, and humic substance carbons fractionated into fulvic acids, humic acids and humin were quantified.Results and discussion
Higher CO2-C emissions occurred in the soil with added residue ranging from 0.5 to 1.1 g CO2-C m?2 h?1 in the first 6 days of incubation, and there was a positive correlation with the less labile organic soil fractions as well as residue type. In the final period, after 12 days of soil incubation, there was a higher relation of CO2-C emission with carbon humin. The sugarcane and soybean residue (20 Mg ha?1) promoted higher CO2-C emission and the reduction of carbon residue. The addition of residue contributed to an 82.32 % increase in the emission of CO2-C, being more significant in the residue with higher nitrogen availability.Conclusions
This study shows that the quality and quantity of residue added to soil affects the carbon sequestration and CO2-C emission. In the first 6 days of incubation, there was a higher CO2-C emission ratio which correlates with the less stable soil carbon compartments as well as residue. In the final period of incubation, there is no effect of quality and quantity of residue added to soil on the CO2-C emission.44.
45.
Abdelfattah Z M Salem German Buenda-Rodrguez Mona M M Elghandour Mara A Mariezcurrena Berasain Francisco J Pea Jimnez Alberto B Pliego Juan C V Chagoyn Mara A Cerrillo Miguel A Rodrguez 《中国农业科学(英文版)》2015,(1)
Anin vitro gas production (GP) technique was used to investigate the effects of combining different doses ofSalix babylonicaextract (SB) with exogenous ifbrolytic enzymes (EZ) based on xylanase (X) and... 相似文献
46.
Angela LO MONACO Luigi TODARO Mara SARLATTO Raffaello SPINA Luca CALIENNO Rodolfo PICCHIO 《中国林学(英文版)》2011,13(4):276-284
This study aims to investigate wood density at different levels of moisture, basic density and shrinkage of timber from Turkey oak (Quercus cerris L.) coppice forests growing in Central Italy. We also studied the variability in density in the trees within and among sites. Density shows no significant statistical differences in the tested population. A higher variability in the shrinkage than in the density was found. Wood moisture is referred to as dry mass and fresh mass, which is related to many performance characteristics of wood, i.e., energy production. Trends in moisture and water content were studied because these physical parameters play an important role in the specific area of firewood which requires an accurate estimation of mass, volume and energy content. This work is a contribution to improve xylo-energy estimates of small and medium forestry issues. 相似文献
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Marilaine Ragugnetti M?nica L. Adams Ana T. B. Guimar?es Graziela Sponchiado Eliane Carvalho de Vasconcelos C��ntia Mara Ribas de Oliveira 《Water, air, and soil pollution》2011,218(1-4):361-364
Medicines and their metabolites have been found as water contaminants at very low concentrations; moreover, there is no extensive toxicological data to determine the risks associated with their occurrence in water resources. The ibuprofen genotoxicity potential to the Oreochromis niloticus fish (Tilapia), due to nanograms per liter exposure, was evaluated using the micronucleus test. Acute (48 h) and sub-chronic assays (10 days) were carried out at 300 ng/L ibuprofen aquatic concentration comparing with the negative control group (without treatment), with eight animals per group. The results were assessed from the average of triplicate analyses. The micronucleus frequency in peripheral blood of fish was determined using a sample size of 3,000 erythrocytes per animal. Significance was defined using t test (p????0.05). The bioassay results showed a statistically significant increase in the frequency of micronuclei for both exposure times in comparison to the negative control. The micronucleus frequency observed for the sub-chronic tests was higher than the one identified in the acute assays. The observed ibuprofen genotoxic effects demonstrated an aquatic environmental risk of this pharmaceutical, which occurs for the used fish experimental model in lower concentration than previously described for other aquatic organisms. 相似文献