全文获取类型
收费全文 | 298篇 |
免费 | 14篇 |
专业分类
林业 | 7篇 |
农学 | 5篇 |
13篇 | |
综合类 | 6篇 |
农作物 | 5篇 |
水产渔业 | 72篇 |
畜牧兽医 | 193篇 |
植物保护 | 11篇 |
出版年
2023年 | 1篇 |
2022年 | 9篇 |
2021年 | 9篇 |
2020年 | 6篇 |
2019年 | 8篇 |
2018年 | 6篇 |
2017年 | 3篇 |
2016年 | 6篇 |
2015年 | 16篇 |
2014年 | 16篇 |
2013年 | 14篇 |
2012年 | 22篇 |
2011年 | 30篇 |
2010年 | 4篇 |
2009年 | 11篇 |
2008年 | 18篇 |
2007年 | 18篇 |
2006年 | 31篇 |
2005年 | 16篇 |
2004年 | 19篇 |
2003年 | 18篇 |
2002年 | 14篇 |
2001年 | 8篇 |
2000年 | 4篇 |
1998年 | 3篇 |
1992年 | 2篇 |
排序方式: 共有312条查询结果,搜索用时 250 毫秒
11.
We have previously reported that the scallop shell extract possesses free radical scavenging activity. In this study, we isolated a glycoprotein with a molecular weight of approximately 90 kDa (named 90-kDa protein) which showed 1,1-diphenyl-2-picrylhydrazyl (DPPH) radical and superoxide anion radical scavenging, reducing, and ferrous ion-chelating activities. Amino acid composition analysis showed that the 90-kDa protein was rich in Asx (Asp or Asn), Ser and Gly residues. A BLAST search of partial amino acid sequences determined by matrix-assisted laser desorption/ionization tandem time-of-flight mass spectrometry revealed that the 90-kDa protein is a novel protein. The 90-kDa protein is a yellow protein that contains fluorescent substances. To the best of our knowledge, this is the first report of a radical scavenging protein from the scallop shell. 相似文献
12.
Hashizume K Takahashi T Shimizu M Todoroki J Shimada A Hirata M Sato T Ito A 《The Journal of reproduction and development》2003,49(1):45-53
In vitro cell culture is a convenient tool for studying cellular mechanisms. In the present study, production of matrix-metalloproteinases (MMPs) in bovine endometrial (containing both epithelial and stromal cells) monolayer cells was examined. Blastocysts attached to the endometrial cells in a monolayer culture were examined for their effects on MMP-2 production. Initial attachment of blastocysts to the monolayer inhibited MMP-2 production by endometrial cells. But once trophoblast cells began to migrate into the endometrial cell layer, MMP-2 production increased, and at the same time MMP-9 production also became evident in the medium. In order to understand how blastocysts affected MMP-2 production, we examined the effect of progesterone, estradiol, insulin-like growth factors (IGFs), tumor necrosis factors (TNFs), and interferon-tau (IFN-tau) supplementation. It was IFN-tau that inhibited the production of MMP-2. In addition, progesterone at a lower dose appeared to inhibit MMP-2 production. Both TNF-alpha and TNF-beta strongly stimulated the production of MMP-2 and MMP-9, whereas IGFs had no effect. Based on these findings, it appears that conceptus has the capacity to inhibit MMP activity. 相似文献
13.
Itakura S Kato H Wang PC Matsunaga A Jitsukawa T Edamura K Ohgawara H Mochizuki M Nishimura R Sasaki N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(10):1107-1109
Complement-mediated cytotoxicity for porcine islet cells (PICs) was evaluated using sera of six animal species. Then soluble complement receptor type-1 (sCR1) as an anti-complement agent was added to those sera, and the changes in 50% hemolytic unit of complement serum (CH50) and cytotoxic effect of those sera on PICs were examined. All the sera except for that of pig showed cytotoxicity. However, the extent of toxicity was considerably different between species. In the rat and human serum, sCR1 significantly reduced CH50 and cytotoxicity, however in the dog serum, sCR1 had no suppressive effects. These results may suggest that complement contribute to humoral cytotoxicity for PICs as a main factor, and the compatibility of complement with PICs differs between animal species. 相似文献
14.
Yin SA Makara M Pan Y Ishiguro H Ikeda M Numakunai S Goryo M Okada K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(5):599-606
Thirty-three cases of enzootic bovine leukosis (EBL) and 14 cases of sporadic bovine leukosis (SBL) were examined by immunohistochemistry using 6 monoclonal antibodies against leukocyte differentiation molecules of bovine leukocytes. There were 17 cases of B-1a cell type, 10 cases of B-1b cell type and 6 cases of B-2 cell type in EBL, and 5 cases originating from B cells (B-2 cell type) and 9 cases originating from immature T cells in SBL. The average age for the EBL cases of B-1a cell type was 8.6 years, B-1b cell type was 6.5 years, and of B-2 cell type was 4.5 years. In cases of SBL, immature T cell type patients were younger than B-2 cell type ones. The lymphoma originating from B cells differed from that originating from T cells in morphology. In T cell tumors, the nucleus of tumor cells was round, the edge of the cytoplasm obvious, and tumor cells were sporadically present and proliferated. When compared with T cells, the region among B cells was obscure. But, there was no relation between phenotype and the histologic classification of tumor cells. In EBL, beyond the lymph node, tumors of B-1a and B-1b types had developed in the heart and abomasum, and those of the B-2 type tended to occur in liver. In SBL, B-2 type and T type cells formed tumors in the liver, kidney, thymus, and one case of T-cell type tumor formed on the skin. We would like to propose a new classification of bovine leukosis as EBL, calf type B-cell lymphoma, juvenile T-cell lymphoma and skin type T-cell lymphoma. 相似文献
15.
16.
Ohashi E Inoue K Kagechika H Hong SH NakagawaTakayuki Takahashi T Mochizuki M Nishimura R Sasaki N 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2002,64(2):169-172
The effect of two natural retinoids and synthetic retinoids with or without retinoid synergists on the proliferation and differentiation of 3 melanoma cell lines were investigated in vitro. No retinoids showed significant growth inhibitory effect on these cell lines when used alone, however, cell differentiation and significant growth inhibition were observed when treated with a combination of retinoids and a retinoid synergist. This study may suggest that, though the cells showed low susceptibilities when retinoids were treated alone, the combination of retinoids and a retinoid synergist may be effective to control the growth of canine melanoma cell lines. 相似文献
17.
Kaoru Hanada Fumiyoshi Fukumoto Manabu Kusunoki Mitsuro Kameya-Iwaki Yuko Tanaka Toru Iwanami 《Journal of General Plant Pathology》2006,72(6):383-386
An undescribed spherical virus ca. 30 nm in diameter was isolated from gladiolus (Gladiolus spp.) plants in Japan. The virus had a moderate host range within eight families. Purified virus preparations contained two
large RNA components and one coat protein with mobility similar to Cycas necrotic stunt virus (CNSV) from cycas (Cycas revolute). The virus was serologically closely related to CNSV. Its nucleotide sequence of the coat protein gene had 89% common identity
with that of CNSV. These results indicated that the virus isolated from gladiolus is a new strain of CNSV.
The nucleotide sequence data reported are available in the DDBJ/EMBL/Gen Bank databases under the accession number AB237656. 相似文献
18.
Akinori Hirashima Manabu Matsushita Kentaro Nakazono Morifusa Eto 《Pesticide biochemistry and physiology》2006,85(1):15-20
Effects of 1-arylimidazole-2(3H)-thiones (AITs) and 1-(substituted benzyl)imidazole-2(3H)-thiones (BITs) were tested on progeny formation in Drosophila melanogaster. Some AITs showed inhibitory activities at laying eggs and delayed eclosion by 1 day. The inhibitory activity was nullified by adding octopamine (OA) or noradrenaline (NA) to the medium for progeny formation in D. melanogaster. The effect of AIT on the contents of OA and NA was analyzed in adults of D. melanogaster by high-performance liquid chromatography with electrochemical detection. Flies fed with AIT decreased OA and NA levels and increased TA content. Taken together, the inhibitory activity of AIT could be due to inhibition of tyramine β-hydroxylase and dopamine β-hydroxylase. 相似文献
19.
Tsukasa OKANO Katsuhi NAKATA Yumiko NAKAYA Takashi NAGAMINE Manabu ONUMA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(6):637-642
The Ryukyu long-furred rat, Diplothrix legata, is a large rodent
distributed only on Amami-ohshima Island, Tokuno-shima Island and Okinawa-jima Island,
Japan. This animal is endangered as a result of deforestation, predation by introduced
carnivores and mortality caused by vehicles. We performed theriogenological examinations
of 32 male and 25 female Ryukyu long-furred rats carcasses collected from wild populations
on northern Okinawa-jima Island from December 2005 to September 2013. Adult males had
remarkably large preputial glands. Seminiferous diameter of adult was significantly small
(136 ± 28 µm, n=8) from April to August. Numerous spermatozoa were
observed from September through February, and seminiferous diameter was significantly
large (216 ± 27 µm, n=12) during this time in adults; testes length
changed in a similar pattern. These findings indicate that the mating season may occur
from September through February. Size (body length) at sexual maturity was estimated to be
>560 mm in both sexes. From observation of corpora lutea and placental scars, litter
size was estimated to range from 2 to 12 (average=6, n=4). These results provide
fundamental knowledge that will be beneficial for in situ and ex
situ conservation of this rare species. 相似文献
20.
MD Shah ALAM Shin-Ichi TESHIMA Manabu ISHIKAWA Shunsuke KOSHIO 《Fisheries Science》2002,68(3):509-516
ABSTRACT: To investigate the effects of disproportionate levels of dietary arginine and lysine on juvenile Japanese flounder Paralichthys olivaceus , growth performance and biochemical parameters were evaluated by feeding five test diets, comprising different levels of arginine and lysine, to triplicate groups of juveniles (initial bodyweight 1.85 g) for 40 days. Crystalline amino acids were supplemented to test diets to correspond to the amino acid pattern found in the whole body protein of the Japanese flounder, except for arginine and lysine. After the feeding trials, plasma arginine, lysine and urea levels, excreted ammonia-N, and liver arginase activity were analysed. Survival, specific growth rate, feed conversion efficiency, and apparent protein retention were adversely affected ( P < 0.05) by the deficiency in dietary arginine or lysine concentrations. An excess of either lysine or arginine in the diet did not depress growth when the diets contained adequate levels of either arginine or lysine, proving that there is no evidence for an arginine–lysine antagonism. Results for plasma arginine, lysine and urea levels, excreted ammonia, and liver arginase activity also demonstrated that Japanese flounder juveniles are not sensitive to excess dietary levels of lysine and arginine. 相似文献