Effect of NO3 and NH4 concentrations in nutrient solution on yield and nitrate concentration in seasonally grown leaf lettuce

The effect of suboptimal supply of nitrogen (N) and of replacing nitrate in the nutrient solution with ammonia on growth, yield, and nitrate concentration in green and red leaf lettuce was evaluated over two seasons (autumn and spring) using multiple regression analysis. The plants were grown in a greenhouse on a Nutrient Film Technique (NFT) system. Nitrogen concentrations in the nutrient solution were either 3?mM or 12?mM, and the form of N was varied as follows: 100% NO₃, 50% NO₃?+?50% NH₄, and 100% NH₄. In both seasons, the biomass (fresh weight) of lettuce heads increased with increasing NO₃ concentrations and in autumn, NO₃ even at 1.5?mM was sufficient for high yield. However, head dry weight was affected neither by the season nor by changes in the composition of the nutrient solution. The concentration of NO₃ had no effect on root dry weight, but it decreased at higher concentrations of NH₄. The number of leaves increased as the ratio of NO₃ to NH₄ in the nutrient solution increased and was higher in autumn because of the longer growth period. Increasing the concentration of NO₃ in nutrient solution increased both total N and nitrate concentration in lettuce heads (dry weight) but decreased the concentration of total C. Also, leaf nitrate concentration was lower in spring than in autumn and decreased with increasing NH₄ concentration. Nitrogen utilization efficiency was maximum when NH₄ levels in the nutrient solution were either 0% or 50% irrespective of the season. Our results thus show that suboptimal N supply in autumn will not affect lettuce yield, and that nitrate concentration in leaves is lower when NH₄ concentrations in nutrient solution are higher and also much lower in red lettuce than in green lettuce.     

Distribution and status of medicinal leeches (genus Hirudo) in the Western Palaearctic: anthropogenic,ecological, or historical effects?

1. Distribution and status of medicinal leeches were re‐considered in the light of the new taxonomy recognizing four Western Palaearctic species: Hirudo medicinalis, Hirudo verbana, Hirudo orientalis and Hirudo troctina. 2. Recent records and new data obtained on expeditions to Ukraine, Russia, Azerbaijan, Kazakhstan, Uzbekistan and the Western Balkans were mapped to obtain an up‐to‐date overview of the distribution. 3. Three hypotheses explaining the current ranges of all Hirudo species were tested. The ecological hypothesis, suggesting a strong impact of large‐scale environmental factors, received the highest support, while anthropogenic influence was minimal, and no historical patterns of refugia and colonization were detected. 4. Mapped localities of all Hirudo species show extensive, belt‐shaped ranges extending from east to west. H. medicinalis is distributed from Britain and southern Norway to the southern Urals and probably as far as the Altai Mountains, occupying the deciduous arboreal zone. H. verbana has been recorded from Switzerland and Italy to Turkey and Uzbekistan, which largely corresponds to the Mediterranean and sub‐boreal steppe zone. H. orientalis is associated with mountainous areas in the sub‐boreal eremial zone and occurs in Transcaucasian countries, Iran and Central Asia. H. troctina has been found in north‐western Africa and Spain in the Mediterranean zone. 5. Based on the data gathered, and considering real and potential threats, global IUCN category Near Threatened is proposed for H. medicinalis, H. verbana, and H. orientalis, while H. troctina can only be assigned to category Data Deficient. Copyright © 2010 John Wiley & Sons, Ltd.     

The European College of Veterinary Pathologists (ECVP): the professional body for European veterinary pathologists

The European College of Veterinary Pathologists (ECVP) was established in 1995 with the aim of advancing veterinary pathology and promoting high standards within the specialty in Europe. The ECVP is one of 21 European colleges recognized by the European Board of Veterinary Specialisation (EBVS), which represents a quality-assurance system for European veterinary specialists. Until the ECVP was founded, there was no unified European system recognizing the specialty of pathology, and many European countries followed their own qualification systems, which varied in form and standard. The ECVP provides an annual certifying examination, the passing of which is required to gain membership (diplomate status) in the college. This qualification is now accepted on equal terms by the well-established American College of Veterinary Pathologists (ACVP). In line with EBVS requirements, the ECVP has also established a standard continuing professional development (CPD) and re-registration system for its membership. Furthermore, it has promoted and unified European post-graduate training in veterinary pathology by setting up requirements for residency training programs and making registration and monitoring of these programs by the ECVP a prerequisite for approval of an institution as a training facility. The concurrent establishment, together with the European Society of Veterinary Pathology, of an annual summer school that trains residents for the certifying examination has further fostered European post-graduate training. Within 10 years, the ECVP has succeeded in establishing common standards and a unified approach to veterinary pathology throughout Europe. This article describes the evolution and organization of the ECVP.     

The effect of pest control on the incidence of trichinosis in Virovitica-Podravina County, Croatia

The aim of the study was to assess the role of pest control as a preventive measure in the control of trichinosis. The investigation was designed as a retrospective cohort study in the Virovitica-Podravina County. The incidence of trichinosis was determined according to the presence or absence of exposure to protective pest control measures. Differences in the rate of trichinosis infection were determined by calculating relative risk (RR), i.e., the ratio of absolute risk in the two groups. In this epidemiological study, data collected in the County area regularly covered by pest control (exposure area) were compared with other areas not covered by regular pest control measures (non-exposure area). The study included 132 subjects living in the County and locally infected with trichinosis. Patient data were obtained from their medical history, epidemiological survey and epidemiological questionnaire on the source (autochthony) of the disease. The study also included 133,028 domestic pigs, 875 wild animals (mostly wild boar) and 111 trapped rats. All samples were examined by the method of trichinoscopy at authorized veterinary institutions of the County. Trichinosis infection was recorded in 1012 of 79,534 domestic pigs in the area exposed to pest control measures and in 51 of 53,494 domestic pigs from the rest of the County. RR for the exposure area vs. non-exposure area was 13.35 (95% CI 10.08-17.68; p<0.05); according to particular areas, RR was 3.66 (95% CI 2.62-5.13) for Pitomaca, 22.47 (95% CI 13.27-38.08) for Slatina and 118.89 (95% CI 29.7-476.01) for Orahovica (p<0.05 all). Domestic pigs from the County areas exposed to regular pest control measures had a 13-fold likelihood of infection recorded in pigs from County areas where pest control measures were not or were only occasionally performed. Quite unexpectedly, these results revealed a failure of pest control to reduce the incidence of trichinosis. The spread of trichinosis obviously occurs by some routes obviating the impact of pest control.     

"肉鸡球虫病及其综合防治措施"专栏(一)应采用轮换用药方案防治家禽球虫病

球虫病是家禽生产者一个永远挥之不去的威胁.无论该病表现为临床型还是亚临床型均具有重要的经济意义,因此应给予不断关注.由于该病的致病原生动物易对特定抗妹虫药产生耐药性,应采取一种有效的轮换用药方案.     

Cardiac troponin I in dogs anaesthetized with propofol and sevoflurane: the influence of medetomidine premedication and inspired oxygen fraction

Objective

To investigate changes in serum cardiac troponin I (cTnI) concentrations in dogs in which medetomidine was used for sedation or for premedication prior to anaesthesia with propofol and sevoflurane.

Temporal trends in Giardia occurrence in the Grand River and surrounding tributaries,Waterloo, Ontario (2005–2013), a retrospective analysis of surveillance data

Giardia contamination in the Grand River Watershed (south‐western Ontario, Canada) was monitored from 2005 to 2013 as part of FoodNet Canada. Our study objectives were to describe the temporal pattern of Giardia occurrence and determine whether water quality parameters and bacterial indicators could act as effective markers for Giardia occurrence. Water samples were collected monthly from the Grand River near a drinking water intake point (2005–2013) and also collected intermittently from other areas in the watershed during the study period. Samples were tested for Giardia cysts using the US EPA method 1623. Samples were also tested for chemical and microbial water quality indicators. Univariable and multivariable linear regression models were built to examine whether temporal, water quality and bacterial indicators were associated with Giardia cyst concentration. Giardia cysts were identified in 89% of samples (n = 228), with highest measured concentrations downstream of a waste water treatment plant outfall. Year and season were found to be predictors for Giardia occurrence. Concentrations were significantly higher in the winter and fall compared to the summer, and significantly higher in 2007 compared to other study years. After controlling for season, year and sampling location, dissolved oxygen was the only variable significantly associated with Giardia cyst concentration. Seasonal peaks in Giardia cyst concentrations in samples collected near the intake for the drinking water plant did not align with the seasonal peak in human Giardiasis cases in this region that are reported annually by public health authorities. This suggests that the risk of contracting Giardiasis from treated drinking water in this community is possibly low when the treatment plant is functioning adequately. Instead, waterborne exposure is likely the result of seasonal behaviours surrounding recreational water use. Therefore, the collective findings of our study are important to help inform future risk management studies and guide public health protection policies.     

The influence of early yield on the accumulation of major taste and health-related compounds in black and red currant cultivars (Ribes spp.)

The focus of our study was to investigate the effect of crop load on the accumulation and composition of primary metabolites (sugars and organic acids), selected groups of flavonoids (anthocyanins and flavonols), and total phenolics in two subsequent years in four black currant cultivars ('Titania', 'Triton', 'Tsema', and 'Cacanska crna') and three red currant cultivars ('Junifer', 'Rolan', and 'Stanza'). For the determination and quantification of compounds, high-performance liquid chromatography-photodiode array with a mass spectrometer was used. Significant differences among cultivars were detected in all analyzed compounds. Anthocyanins were the predominant phenolic group and were more abundant in black currant cultivars as compared to red ones. Similar amounts of sugars and organic acids were measured in both Ribes species; however, vitamin C was 3-fold higher in black currants. A larger crop load in the second year had a negative effect on the sugar content of berries and promoted a higher degree of acids, with the exception of vitamin C, which was higher in the year with a lower crop load. On the other hand, the content of anthocyanins and flavonols was higher in the year with a larger crop load, while there were no differences in total phenolic content.     

Generalized Taenia crassiceps cysticercosis in a chinchilla (Chinchilla lanigera)

Taenia crassiceps is a cestode parasite that uses carnivores as definitive hosts and rodents and rabbits as main intermediate hosts, but other animal species and humans may also get infected. One adult male chinchilla (Chinchilla lanigera) from an animal shelter in Switzerland presented widespread subcutaneous fluctuant swellings extended over the forehead, nose, face and thoracic regions with a progressive growth over 3 months. The thoracic swelling was surgically resected, and it consisted of numerous 3–4 mm small transparent vesicles, mainly confined to the subcutaneous tissue, which were morphologically identified as cysticerci of T. crassiceps. The diagnosis was confirmed by PCR and DNA sequence analysis of fragments of the mitochondrial small subunit rRNA and NADH dehydrogenase subunit 1 genes. After 1.5 months, due to enlargement of the swollen areas and deterioration of the general health condition, the chinchilla was euthanized and a necropsy was performed. Thousands of small cysticerci were observed widespread in the subcutis, involving underlying musculature of the whole body, in the thoracic cavity, larynx, pharynx and in the retropharyngeal region. Additionally, three larger metacestodes were detected in the liver and morphologically and molecularly identified as Taenia taeniaeformis strobilocerci. The present case represents an indicator of the environmental contamination with Taenia eggs, highlighting the risk of infection for susceptible animals and humans. Besides the clinical relevance for pets, T. crassiceps is a zoonotic parasite and can be also cause of severe cysticercosis in humans.