Effect of seed phosphorus concentration on nodulation and growth of three common bean cultivars

Abstract

An experiment was conducted in the greenhouse to evaluate the effects of seed phosphorus (P) concentration on growth, nodulation, and nitrogen (N) and P accumulation of three common bean (Phaseolus vulgaris L.) cultivars. Seeds were produced under low or high soil P levels, and soaked, or not, in 200 mM KH₂PO₄ solution. The experiment had a 3×3×2×2 factorial block design: three cultivars (ICA Col 10103, Carioca and Honduras 35), three levels of applied P (15, 30 and 45 mg P kg^?1 soil), two native seed P concentrations, and two seed soaking treatments. Plants were harvested at flowering. Soaked seeds increased the number, dry mass and P content of nodules, but did not affect plant growth. Plants originated from seeds with high native P concentration presented higher shoot dry mass and nodule number and mass at every soil P level, and were less responsive to increased soil P supply, than plants from low seed P. In plants from seeds with high P, soil P levels did not alter significantly root dry mass, while in plants from seeds with low P bean cultivars expressed wider differences in root dry mass. The genotypic variability of nodulation was influenced by soil P levels and seed P concentration. Both higher soil or seed P supply enhanced N and P accumulation in shoots. These results indicate that a high seed P concentration produces plants less dependent on soil P supply, and can enhance nodulation and N₂ fixation of common bean. Seed P supply affected the cultivar performance, and should be considered in evaluation of bean genotypes.     

Soil microbiological attributes under summer/winter crops rotation in a no-tillage system

Knowledge of the effect of a multiple combination of summer/winter crop rotation on the microbiological properties of soil would allow a more adequate response to its use. This study aimed to evaluate the effect of the rotation of three summer crops (continuous soybean, continuous maize and soybean/maize rotation) in combination with seven winter crops (maize, sunflower, oilseed radish, millet, pigeon pea, sorghum and sunn hemp) on the microbiological properties of the soil. A soybean/maize (SM) rotation had a greater influence on microbial biomass than continuous maize (MM) and continuous soybean (SS). Urease and phosphatase activities were not affected by the crop rotation. Dehydrogenase activity was higher in continuous crops (MM and SS) than in SM, whereas respiratory activity was higher in SM than in continuous crops. For the SM rotation, the main variables selected by principal components analysis were microbial biomass C, N and P, respiratory and phosphatase activities, and microbial quotient. Pigeon pea, sorghum and sunn hemp had a greater effect on soil properties than the other winter crops. In general, the degree of influence of the summer and winter crops on the microbiological soil properties can be ranked as follows: SM > MM > SS, and millet > sorghum > sunn hemp > radish > pigeon pea > maize, respectively.     

Neural networks in climate spatialization and their application in the agricultural zoning of climate risk for sunflower in different sowing dates

ABSTRACT

Sunflower is a species that is sensitive to local climate conditions. However, studies that use artificial neural networks (ANNs) to evaluate this influence and create tools such as agricultural zoning of climate risk (ZARC) have not been conducted for this species. Due to the importance of sunflower as a human food source and for biodiesel production, and also the necessity of conducting research to evaluate the suitability of this oleaginous species under different climatic conditions. Thus, we seek to construct a ZARC for sunflower in Brazil simulating sowing on different dates and using meteorological elements spatialized by ANNs. Climate data were used: air temperature (T), rainfall (P), relative air humidity (UR), solar radiation (MJ_m^?2_d^?1) and wind velocity (U²). Climatic regions considered suitable for the cultivation of sunflower had average annual values for T between 20 and 28°C, P between 500 and 1.500 mm per cycle, and soil water deficit (DEF) below 140 mm per cycle. A neural network is an efficient tool that can be used in spatialization of climate variables quickly and accurately. Sunflower sowing in the spring and summer are the ones that provide the largest suitable areas in southeastern Brazil, with 58.13 and 64.36% of suitable areas, respectively.     

Plant development,agronomic performance and nutritive value of forage maize depending on hybrid and marginal site conditions at high latitudes

Abstract

The objectives of this investigation were to study the effects of marginal site conditions and hybrid on plant development, agronomic performance and nutritive characteristics of forage maize (Zea mays L.) at high latitudes. Field experiments were conducted in 2008 and 2009 at three experimental sites, Kristianstad, Skara and Västerås, at increasing latitudes from 55°–60° N. Experimental design used two replicated randomized complete blocks at each site with three maize hybrids, Avenir (FAO 180), Isberi (FAO 190) and Burli (FAO 210), which were continuously assessed for plant development and harvested at various levels of maturity. The chemical composition and nutritional characteristics of harvested plant materials were analysed and hybrid responses to advancing maturity in terms of yield and nutritional qualities were evaluated. Results showed that maize hybrids required different numbers of accumulated thermal units at sites on varying latitudes to achieve developmental stages. Lowest thermal unit requirements among hybrids were observed for hybrid Avenir, and for sites it was highest for plants grown in the most northern site, Västerås. The most southern site, Kristianstad, was the only site at which all hybrids reached the dent stage (c. 450 g kg^?1 kernel DM), a recommended maturity for ensiling. The DM yields of early maturing hybrid Avenir were consistently lower than those for Isberi and Burli at all the sites. Results also revealed nutritional differences among maize hybrids at a given maturity (DM, g kg^?1), indicating that the effects of maturation should be factored into design of hybrid performance trials. This study highlights the effects of marginal site conditions and hybrids on plant development, agronomic performance and nutritional characteristics of maize hybrids at high latitudes. Further studies on marginal sites are recommended to enlighten the understanding of interaction between environmental and genetic factors on the performance of forage maize.     

Fate of humic acids isolated from natural humic substances

Composition of humic acids (HA) is a function of plant-derived inputs, degradation processes regulated by microorganisms, organo-mineral interactions and age. Characterization of different origin humic substances is important for evaluation of their contribution to stabile and labile carbon pool in the environment. The relative abundance of chemical components in HA isolated from soils, compost, commercial lignohumates, alginite, acadiane and lignite was studied with aim to quantify content of important biomarkers such as amino acid, lipids and polyphenols. HA were considered as a heterogeneous complex and high concentration of peptides, polyphenols and lipids was determined in acadian-HA to compare with soil-HA. Compost-HA contained much more amino acids to compare with soil-HA samples. Alginite-HA and lignite-HA were similar in biomarkers content to soil-HA. Fourier transform infrared spectroscopy confirmed that chemical composition and functional groups content differs with the origin, humification degree and the age of studied samples. Soil-HA are typically composed of a variety of ?OH, COOH?, C–O, C–H₂, (aliphatic and aromatic) groups, quinines, lignin fragments, polysaccharide, monosaccharide and proteins fragments, which are linked together by ?O?, ?NH?, ?H=, >C=O, metal ions and –S? groups. ¹³C NMR spectroscopy showed that aromatic carbon content was the highest in lignite-HA and soil-HA.     

Variability in sensory attributes in common bean (Phaseolus vulgaris L.): a first survey in the Iberian secondary diversity center

Few studies have explored sensory variability in beans. We used geographic, agronomic, morphological, and genetic criteria to select numerous groups of landraces from among the many collected in Catalonia (northeast Spain). Beans cultivated in a single location to avoid environmental effects were submitted to a trained panel for sensory analysis. We used multiple linear regression to explore whether the flavor and texture of the seed (evaluable only by tasting after cooking) can be estimated from easily measurable traits like the appearance of the beans. The degree of variability found for all sensory traits (coefficients of variation: 20–70 %) was similar to that of agronomic traits; however, entries were grouped differently for sensory and agronomic traits, suggesting independent evolution. Negative genotypic correlations between some sensory traits will probably hinder attempts to achieve ideotypes combining low seed-coat perceptibility, low mealiness, and high flavor. Regressions between easily measurable traits (agronomic and culinary traits and appearance of the seeds) and flavor and texture yielded R² values ranging from 0.47 to 0.64. We consider these values good enough for rough screening of sensory attributes in genetic resources studies or breeding.     

Modulation of Uptake and Translocation of Iron and Copper from Root to Shoot in Common Bean by Siderophore-Producing Microorganisms

Microbes have developed high-affinity uptake mechanisms to assimilate iron (Fe) and other metals such as aluminum (Al), gallium (Ga), chromium (Cr), and copper (Cu). Siderophores, which are metal chelating compounds, and membrane receptor proteins are involved in these specialized mechanisms. A few siderophore-producing microorganisms associated with plant roots also influence the uptake of some metals. In this study, the potential microbial-assisted Cu and Fe uptake by Phaseolus vulgaris (common bean) plants was evaluated. Seedlings of cultivated common bean varieties Bayo-INIFAP (B) and Negro-150 (N) and wild types yellowish (WY) and black (WB) were developed in the presence of a Cu and Fe solution and associated with the siderophore-producing microorganisms R. leguminosarumbv. Phaseoli (strains 19, 44, and 46); Pseudomonas fluorescens(strain Avm), and Azospirillum brasilense (strain 154). Seedlings of cultivated variety N and black wild type WB inoculated with the strain CPMex.44 accumulated 71% and 30% more Fe than the un-inoculated plants, respectively; however, the wild black bean accumulated the highest absolute amount of Fe (221.56 mg/kg of dry matter) as compared with the cultivated black variety N (126.16 mg/kg of dry matter) (P < 0.05). In the wild type WY seedlings, the highest Fe accumulation was observed when the seeds were inoculated with the Pseudomonas strain Avm (206 mg/kg of dry matter) (P < 0.05). The interaction of Pseudomonas strain Avm with seedlings of the cultivated B variety and the wild type WB promoted the highest accumulation of Cu (51 and 54 mg/kg of dry matter, respectively), 7 and 14 mg more than in the respective non-inoculated seedlings. No promotion of Fe accumulation was observed in the seedlings of the cultivated B variety and in roots; instead, less Fe was accumulated. The wild type WY did not show any improvement in Cu accumulation. In this study, Rhizobiumstrains promoted Fe but not Cu uptake in P. vulgaris seedlings while Pseudomonas strains promoted the uptake of both Cu and Fe.     

Effect of Excess Cadmium and Zinc Ions on Roots and Shoots of Maize Seedlings

The effect of 8-day-old exposure maize seedlings with cadmium (Cd) or zinc (Zn), separately, are described with special attention being given to ultrastructural changes as well as changes of the growth. These elements, frequent pollutants found in the soil, were added as 1 mM solutions to nutrient solution used for roots and shoots of maize seedlings, investigated 8 days after germination. The symptoms of heavy metal toxicity were clear showing that Cd inhibits root growth more strongly than the shoots and more effectively than zinc ions. The results of scanning electron microscopy revealed changes in the leaf surface, particularly in the guard cells of the stomata. The ultrastructural analyses of the parenchyma mesophyll cells showed extensive chloroplast disorganization, mainly affecting the thylakoid membranes and grana.     

ADVERSE EFFECTS OF HUMIC SUBSTANCES FROM DIFFERENT ORIGIN ON LUPIN AS RELATED TO IRON SOURCES

Humic substances improve the efficiency of different iron (Fe) sources overcoming Fe deficiency chlorosis of plants. However, applied at high rates, they can promote negative effects on plants. The main objective of this work was to study the potential adverse effect of three humic acids from different origin when they were applied with two effective Fe sources for plants: Fe- ethylenediaminedihydroxyphenylacetic acid (EDDHA) and Vivianite. To this end, an experiment with lupin (Lupinus albus L.) was performed involving two factors: (i) Fe source, and (ii) humic substances from three different origin (composted cork, leonardite, and compost obtained from a mixture of olive husk with cotton gin trash) applied at 0, 0.1, and 0.5 g organic carbon (C) kg^?1 of growing media. At the rates used, humic substances promoted adverse effects on plant development, chlorophyll meter readings, and Fe content in lupin grown in calcareous media. Overall, the effect on dry matter and Fe content in plants was more relevant when Fe was supplied with Vivianite, the effect on chlorophyll meter readings being more significant when Fe was applied as Fe-EDDHA. Differences were also observed depending on the source of humic substances, those from leonardite promoting the greatest decrease in dry matter in roots and shoots. These humic substances possessed the highest values of spectroscopy index for aromaticity (A₂₅₄ ). On the other hand, the application of humic substances from olive husk compost, which exhibited the lower aromaticity index, resulted in the smallest decrease in dry matter production and chlorophyll meter readings. Dry matter in roots decreased logarithmically with increased values of the estimates of the amounts of aromatic compounds accumulated in the growing media (R² = 0.92; P < 0.01) with Vivianite as Fe source. Thus, the effects decreasing dry matter production, particularly in roots, and chlorophyll meter readings can be ascribed at least partially to the presence of phytotoxic aromatic compounds in humic substances.     

BORON UPTAKE AND DISTRIBUTION IN FIELD GROWN CITRUS TREES

In low fertility tropical soils, boron (B) deficiency impairs fruit production. However, little information is available on the efficiency of nutrient application and use by trees. Therefore, this work verified the effects of soil and foliar applications of boron in a commercial citrus orchard. An experiment was conducted with fertigated 4-year-old ‘Valencia’ sweet orange trees on ‘Swingle’ citrumelo rootstock. Boron (isotopically-enriched ¹⁰B) was supplied to trees once or twice in the growing season, either dripped in the soil or sprayed on the leaves. Trees were sampled at different periods and separated into different parts for total B contents and ¹⁰B/¹¹B isotope ratios analyses. Soil B applied via fertigation was more efficient than foliar application for the organs grown after the B fertilization. Recovery of labeled B by fruits was 21% for fertigation and 7% for foliar application. Residual effects of nutrient application in the grove were observed in the year after labeled fertilizer application, which greater proportions derived from the soil supply.