Apparent Electrical Conductivity,Soil Properties and Spatial Covariance in the U.S. Southern High Plains

Site-specific soil and crop management will require rapid low-cost sensors that can generate position-referenced data that measure important soil properties that impact crop yields. Apparent electrical conductivity (EC_a) is one such measure. Our main objective was to determine which commonly measured surface soil properties were related to EC_a at six sites in the Texas Southern High Plains, USA. We used the Veris 3100 and Geonics EM-38 EC mapping systems on 12 to 47 ha areas in six center-pivot irrigation sites. Soil samples were taken from 0–150 mm on a 0.1 to 0.8 ha grid and analyzed for routine nutrients and particle size distribution. At four of the six sites, shallow EC_a measured with the Veris 3100 (EC_a-sh) positively correlated to clay content. Clay content was negatively related with EC_a-sh at one site, possibly due to low bulk density of the shallow calcic horizon at that site. Other soil properties that were often correlated with EC_a included soil extractable Ca²⁺, Mg²⁺, Na⁺, CEC, silt and soluble salts. Extractable K⁺, NO₃^–, SO₄^–, Mehlich-3-P, and pH were not related to EC_a. Partial least squares regression (PLS) of seven soil properties explained an average of 61%, 51% and 37% of the variation in observed shallow EC_a-sh, deep EC_a with the Veris 3100 (EC_a-dp) and EC_a with the Geonics EM-38 (EC_a-em), respectively. Including nugget, range and sill parameters from a spherical semivariance model of the residuals from PLS regression improved the fit of mixed models in 15 of 18 cases. Apparent EC, therefore can provide useful information to land-users about key soil properties such as clay content and extractable Ca²⁺, but that spatial covariance in these relationships should not be ignored.     

Efficacy of a pandemic (H1N1) 2009 virus vaccine in pigs against the pandemic influenza virus is superior to commercially available swine influenza vaccines

In April 2009 a new influenza A/H1N1 strain, currently named "pandemic (H1N1) influenza 2009" (H1N1v), started the first official pandemic in humans since 1968. Several incursions of this virus in pig herds have also been reported from all over the world. Vaccination of pigs may be an option to reduce exposure of human contacts with infected pigs, thereby preventing cross-species transfer, but also to protect pigs themselves, should this virus cause damage in the pig population. Three swine influenza vaccines, two of them commercially available and one experimental, were therefore tested and compared for their efficacy against an H1N1v challenge. One of the commercial vaccines is based on an American classical H1N1 influenza strain, the other is based on a European avian H1N1 influenza strain. The experimental vaccine is based on reassortant virus NYMC X179A (containing the hemagglutinin (HA) and neuraminidase (NA) genes of A/California/7/2009 (H1N1v) and the internal genes of A/Puerto Rico/8/34 (H1N1)). Excretion of infectious virus was reduced by 0.5-3 log(10) by the commercial vaccines, depending on vaccine and sample type. Both vaccines were able to reduce virus replication especially in the lower respiratory tract, with less pathological lesions in vaccinated and subsequently challenged pigs than in unvaccinated controls. In pigs vaccinated with the experimental vaccine, excretion levels of infectious virus in nasal and oropharyngeal swabs, were at or below 1 log(10)TCID(50) per swab and lasted for only 1 or 2 days. An inactivated vaccine containing the HA and NA of an H1N1v is able to protect pigs from an infection with H1N1v, whereas swine influenza vaccines that are currently available are of limited efficaciousness. Whether vaccination of pigs against H1N1v will become opportune remains to be seen and will depend on future evolution of this strain in the pig population. Close monitoring of the pig population, focussing on presence and evolution of influenza strains on a cross-border level would therefore be advisable.     

An Archean Geomagnetic Reversal in the Kaap Valley Pluton, South Africa

The Kaap Valley pluton in South Africa is a tonalite intrusion associated with the Archean Barberton Greenstone Belt. Antipodal paleomagnetic directions determined from the central and marginal parts of the pluton record a geomagnetic reversal that occurred as the pluton cooled. The age of the reversal is constrained by an 40Ar/39Ar plateau age from hornblende at 3214 +/- 4 million years, making it the oldest known reversal. The data presented here suggest that Earth has had a reversing, perhaps dipolar, magnetic field since at least 3.2 billion years ago.     

Spectral Detection of Emergence in Corn and Cotton

Multispectral reflectance of emerging cotton (Gossypium hirsutum) and corn (Zeamays) seedlings was measured during the 2000 and 2001 growing seasons. Reflectance in blue, green, red, and near infrared (NIR) wave lengths was used to detect seedling emergence, to monitor leaf area growth, and to measure the effect of bare soil reflectance on scene (bare soil and seedlings) reflectance. Cotton and corn seedlings were detected 1 day after initial emergence (1 DAE) in 2000 by the red band. The red band detected seedlings in 2001 at 9 and 8 DAE in early and late planted corn, respectively, and on 0 DAE for cotton. The normalized difference vegetative index (NDVI) detected seedlings at 1 DAE or 2 DAE in both years. Seedling ground cover in 2000 on the initial detection date in the target areas averaged 1.3% and 0.9%, respectively, for cotton and corn; comparable values in 2001 for cotton, early planted corn, and late planted corn, were 1.4%, 0.4%, and 0.8%, respectively. The red wave band was the most sensitive single band for detecting the presence of seedlings, but NDVI was the most sensitive spectral indicator, which was apparently due to the red band since the NIR band did not always detect seedlings. Seedling leaf area was linearly correlated with NDVI values beginning at 1 or 2 DAE. Bare soil was the major component of the scene during stand establishment and dominated single band reflectance and NDVI values. A dry soil surface that was smoothed and sealed by rain usually caused single band reflectance to increase. The high variability in spectral characteristics of bare soil restricted the interpretation of the spectral data to concluding whether or not seedlings were emerging, but without estimating numbers and seedling size.     

Experimental transmission of epizootic bovine abortion (foothill abortion)

Advances in defining the biology of epizootic bovine abortion (EBA), including identification of the etiologic agent, have been hampered by the inability to reproduce the disease with confidence. Experimental reproduction of EBA, by feeding the tick vector Ornithodoros coriaceus on susceptible pregnant heifers, is not reliable. The primary objectives of this study were to identify specific tissue(s) obtained from EBA-infected fetuses that could transmit the disease, and then utilize such an infectious challenge system to better define the pathogen, host immunity and geographic distribution of the agent. Described here is the ability to routinely reproduce EBA following inoculation of cryopreserved suspensions of homogenized thymus into susceptible pregnant heifers. This challenge system permitted experiments demonstrating the agent was non-filterable, inactivated upon sonication and susceptible to antibiotics. These findings suggest a prokaryotic microbe and represent a major advance in EBA research. Additional experiments demonstrated that inoculation of the cryopreserved EBA-infectious tissue into heifers, prior to breeding, conferred immunity. Furthermore, such immunized heifers were resistant to challenge with heterologous sources of infectious tissue, suggesting monovalent vaccine development might be feasible. Lastly, challenge studies employing animals from Central Nevada, an area considered free of EBA, demonstrated partial immunity, suggesting the pathogen, and possibly the disease, enjoy a broader distribution than previously thought.     

Salinity sensitivity of sorghum at three growth stages

Summary The relative salt tolerance of two sorghum cultivars [Sorghum bicolor (L.) Moench., cvs. Northrup King 265 and Asgrow Double TX] at three different stages of growth was determined in a greenhouse experiment. Plants were grown in sand cultures irrigated four times daily with modified Hoagland's solution. A nonsaline solution and six solutions salinized with NaCl and CaCl₂ (2: 1 molar ratio) provided treatments with osmotic potentials (_s) ranging from –0.05 to –1.05 MPa. The saline treatments were imposed for 30 days beginning at either Stage 1, 4, or 7 as defined by Vanderlip and Reeves (Agron J. 64:13, 1972). The 30-day stages are referred to here as the vegetative, reproductive and maturation stages although the first stage may have included initial panicle differentiation. Both cultivars were most sensitive to salinity during the vegetative stage and least sensitive during maturation. Based on a nonlinear least-squares analysis, grain yield reductions of 50% were predicted at _s=–0.68, –1.02, and –1.14 MPa for NK265 and at –0.62, –1.00, and –1.10 MPa for Double TX when salinized during the vegetative, reproductive, and maturation stages, respectively. Although salinity had no significant effect on mean kernel weights, significant growth stage effects and interaction indicated that kernels were heaviest for plants salinized during the vegetative stage. Stover yields were significantly reduced by salination during the vegetative stage but were unaffected when plants were salinized during the maturation stage. Salination during the reproductive stage also decreased stover yield of Double TX but the effect was smaller than that during the first stage. Stover yield of NK265 was unaffected by salinity at this stage.Mineral analysis of the first leaf below the flag leaf at harvest indicated that both cultivars tended to exclude Na from the upper leaves. Ca and Cl concentrations increased with increased salinity in plants salinized during the maturation stage but salination in earlier stages decreased Ca concentration of this upper leaf at harvest and had no effect on the final Cl concentration. Phosphate and K concentrations decreased when plants were salinized during the third stage but increased when plants were salinized during the vegetative and reproductive stages. Mg was unaffected by salinization during the first and last stage but decreased when plants were salinized,during the reproductive stage. An extensive data base now exists which describes the salt tolerances of many different crops (Maas and Hoffman 1977; Maas 1986). These data express yield responses as a function of the average salt concentration in the rootzone. Generally, these data apply only if salinity is fairly uniform from the seedling stage to maturity. Except for germination, little information exists on the tolerances of crops at different stages of growth. Such information could be invaluable to optimize the use of limited water resources. Knowledge that crops are more tolerant during some stages of growth will improve new strategies for utilizing saline drainage waters (Rhoades 1984).Several studies indicate that tolerances do change as the crop develops and matures, but none of these studies completely separated the effects of duration of treatment from the stage of growth that the crop was treated (Ayers et al. 1952; Kaddah and Ghowail 1964; Kovalskaia 1958; Lunin et al. 1961 a, 1961 b; Maas et al. 1983; Ogo and Sasai 1955; Piruzyan 1959; Verma and Bains 1974). Comparisons of sensitivity during specific phenological stages are confounded when treatment periods are of unequal duration.This study was initiated to determine the sensitivity of grain sorghum [Sorghum bicolor (L.) Moench] to salinity during three 30-day periods of growth. Francois et al. (1984) recently reported that sorghum is a moderately salt-tolerant crop. In field plot tests, grain yields of two cultivars decreased 16% per unit increase in salinity (electrical conductivity of saturated soil extracts from the rootzone) above 6.8 dS/m. They further reported that both cultivars were significantly more tolerant at germination than at later stages of growth. Soil water salinities above 8.2 dS/m delayed germination but full germination occurred within 10 days at salinities up to 22 dS/m. Treatments in the present study were designed to assess plant growth and yield responses to 30-day exposures to salinity beginning at either the 2-leaf stage, at the beginning of rapid culm elongation, or after anthesis.     

Irrigation water quality options for corn on saline,organic soils

Summary Corn production on the organic soils of the Sacramento-San Joaquin Delta of California was affected by the salinity of the irrigation water and the adequacy of salt leaching. Full production was achieved on soils that were saline the previous year, provided the electrical conductivity of the irrigation water (EC_i) applied by sprinkling was less than about 2 dS/m and leaching was adequate from either winter rainfall or irrigation to reduce soil salinity (ECM_SW) below the salt tolerance threshold for corn (3.7 dS/m). For subirrigation, an EC_i up to 1.5 dS/m did not decrease yield if leaching had reduced ECM_SW below the threshold. If leaching was not adequate, even nonsaline water did not permit full production. In agreement with previous results obtained in a greenhouse, surface irrigation with water of an electrical conductivity of up to 6 dS/m after mid-season (end of July) did not reduce yield below that of treatments where the salinity of the irrigation water was not increased at mid-season. Results also reconfirm the salt tolerance relationship established in the previous three years of the field trial. The earlier conclusion that the irrigation method (sprinkler or subirrigation) does not influence the salt tolerance relationship was also confirmed.This project was sponsored jointly by the California State Water Resource Control Board, the California Department of Water Resources, the University of California, and the Salinity Laboratory of the US Department of Agriculture     

Salt tolerance of irrigated guayule

Summary The salt tolerance of guayule (Parthenium argentatum Gray cv. N565-II) was tested in small held plots (silty clay soil) in the Imperial Valley of California. Seedlings were transplanted in October 1981. Differential salination was begun in March 1982 and continued for 4 years by irrigating with waters salinized with NaCl and CaCl₂ (1:1 by wt.) to obtain electrical conductivities of 0.8, 1.4, 3, 6, 9, and 12 dS/m. Dry matter, rubber, and resin yields were determined from pollarded plants in February 1984 and uprooted plants in February 1985 and 1986. Rubber concentrations in the woody branches in 1984 and 1985 averaged 6.1 and 7.3%, respectively on a dry weight basis and were not significantly affected by soil salinity. Resin concentrations averaged 8.6% and 7.3% for the two years. In 1986, both rubber and resin concentrations decreased with increased salinity. Rubber and resin concentrations in the root crowns were approximately one percentage point less than those of the shoot. Dry matter and resin yields were not affected by salinity until the time- and depth-averaged electrical conductivity of the saturated-soil extracts ( ) taken from the rootzone (0–90 cm) exceeded 8.7 dS/m. Above 8.7 dS/m, both yields decreased 11.6% per dS/m increase in . Rubber yields decreased 10.8% per dS/m above a threshold of 7.8 dS/m. Plant mortality rather than growth reduction at high levels of salinity appears to be the limiting factor for rubber production from irrigated guayule.     

Salt sensitivity of wheat at various growth stages

Summary The relative salt tolerance of two wheat species (Triticum aestivum L., cv. Probred and Triticum turgidum L., Durum Group, cv. Aldura) at different stages of growth was determined in a greenhouse experiment. Plants were grown in sand cultures that were irrigated four times daily with modified Hoagland's solution. Salinization with NaCl and CaCl₂ (2:1 molar ratio) provided seven treatment solutions with osmotic potentials ( _s ) ranging from –0.05 to –1.25 MPa (electrical conductivities of 1.4 to 28 dS/m). Salt stress was imposed for 45 days beginning at either 10, 56, or 101 days after planting. The three 45-day stages are referred to here as the vegetative, reproductive, and maturation stages although the first stage included spikelet differentiation. In a separate experiment, seedling growth was measured after 21 days of salt stress ( _s = –0.05 to –0.85 MPa) initiated at 0, 7, 11, and 16 days after planting. Salt stress ( _s = –0.65 MPa) delayed germination by 4 days for both wheats but full emergence occurred. Relative growth response curves of the seedlings were alike regardless of whether salt stress was imposed at planting or at the 1st, 2nd, or 3rd-leaf stage of growth. Salt stress also retarded leaf development and tillering but hastened plant maturity. Grain yields from plants stressed during either the vegetative, reproductive, or maturation stages indicated that both species became less sensitive to salinity the later plants were stressed. Grain yield was reduced 50% at _s = –0.76, –1.53, and –1.58 MPa for Probred and –0.65, –1.08, and –1.34 MPa for Aldura when salinized during stages 1, 2, and 3, respectively. Salinity reduced grain yield by reducing seed number more than seed weight indicating that salt stress during stage 1 affected spikelet differentiation. Straw yield was significantly reduced by salt stress only during stage 1. Leaf mineral analyses revealed that Aldura readily accumulated Na whereas Probred did not. Both species accumulated Cl but the concentrations were much higher in Aldura. K uptake was severely inhibited by salt stress imposed during the first stage but not when imposed the second stage.