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Vitis vinifera L. cv. Pinot blanc was grown in both pot and commercial vineyard under lime‐stress conditions. Dark green to very chlorotic young leaves were sampled in order to analyze chlorophyll and iron (Fe) concentration and content, area, fresh and dry weight, specific weight per area. Iron content per individual leaf was a good parameter to separate dark green leaves from chlorotic ones in both trials, while Fe concentration on DW basis was not. Chlorosis occurrence always decreased leaf dry weight and specific weight per area. 相似文献
53.
Resistance to blackleg and tuber soft rot in sexual and somatic interspecific hybrids with different genetic background 总被引:1,自引:0,他引:1
Domenico Carputo Teodoro Cardi Mario Speggiorin Astolfo Zoina Luigi Frusciante 《American Journal of Potato Research》1997,74(3):161-172
Diploid interspecific hybrids from crosses betweenSolanum tuberosum haploids andS. canasense, S. multidissectum, andS. tarijense, along with tetraploid and hexaploid somatic hybrids S.tuberosum (+)S. commersonii, were screened for resistance to blackleg and tuber soft rot byErwinia carotovora subsp.atroseptica andE. carotovora subsp.carotovora. Among the diploid hybrids, those involving clone mlt la of S.multidissectum and tar 2b ofS. tarijense yielded the highest number of genotypes resistant to both blackleg and tuber soft rot. By contrast, all the hybrids involving clone tar 1lb ofS. tarijense were susceptible to both bacterial diseases. As far as resistance of somatic hybrids is concerned, the most interesting genotype was the tetraploid one which showed resistance to both blackleg and tuber soft rot Significant correlations were found betweenE. carotovora subsp.atroseptica andE. carotovora subsp.carotovora both in resistance to blackleg and in resistance to tuber soft rot, suggesting that resistance to the two subspecies is controlled by the same gene/s or that the relevant genes are linked. On the other hand, correlations between blackleg and tuber soft rot were never significant, indicating that different mechanisms may control resistance in tuber and stems. Alternatively, the resistance could be controlled by the same gene/s under a different spatial/temporal expression pattern. Twelve diploid sexual hybrids and one tetraploid somatic hybrid were selected for resistance to blackleg and/or tuber soft rot and for tuber characteristics. 相似文献
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Romina Beleggia Cristiano Platani Giuseppe Spano Massimo Monteleone Luigi Cattivelli 《Journal of Cereal Science》2009
Although pasta is generally not considered for its aromatic properties, some evidence proves that cereal flours release volatile compounds and they might have an effect on the aroma of the transformed products. This work reports on the characterization of the volatile components of semolina and pasta obtained from four durum wheat cultivars (Triticum durum Desf., cvs. PR22D89, Creso, Cappelli, Trinakria). Semolina samples were characterized through polar metabolite profiling and fatty acid analysis to identify potential precursors of the volatile components. The results show significant differences among the samples tested with cv. Trinakria characterized by the highest content of sugars and fatty acids. Volatile composition was investigated both in semolina and in cooked pasta using headspace solid-phase micro-extraction (HS-SPME) and identified by GC–MS. Thirty-five volatile compounds including aldehydes, ketones, alcohols, terpenes, esters, hydrocarbons and a furan were identified. Significant differences were observed between semolina and pasta samples in terms of composition and amount of the volatile compounds. During cooking an increase in aldehyde content, the appearance of ketones and a decrease in alcohol content were observed. Correlations between metabolites and volatiles demonstrate that the flavour of cooked pasta may differ significantly depending on the durum wheat cultivar employed. 相似文献
56.
Stefano Pavan Zheng Zheng Mariya Borisova Petra van den Berg Concetta Lotti Claudio De Giovanni Pim Lindhout Hans de Jong Luigi Ricciardi Richard G. F. Visser Yuling Bai 《Euphytica》2008,162(1):91-98
The recessive gene ol-2 confers papilla-associated and race-non-specific resistance to tomato powdery mildew caused by Oidium neolycopersici. In order to facilitate marker assisted selection (MAS) in practical breeding programmes, we identified two simple sequence repeat (SSR) markers and one cleaved amplified polymorphic sequence (CAPS) marker which are linked to the resistance locus and co-dominantly inherited. Aiming to provide a base for ol-2 positional cloning, we used a large segregating F2 population to merge these markers with all the ol-2 linked amplified fragment length polymorphism (AFLP®) markers previously identified in an integrated genetic map. By screening a tomato bacterial artificial chromosome (BAC) library, we detected two BAC clones containing two expressed sequence tags (ESTs) homologous to the gene mlo, responsible for powdery mildew resistance in barley, as well as an ol-2-linked marker. Chromosomal mapping by Fluorescence in situ Hybridization (FISH) revealed major signals of the two BAC DNAs in the pericentromeric heterochromatin of the short arm of chromosome 4, in the same region where the ol-2 gene was previously mapped. The genetic and cytogenetic co-localisation between ol-2 and tomato mlo-homologue(s), in addition to the similarity of ol-2 and mlo resistances for both genetic and phytopathological characteristics, suggests that ol-2 is likely a mlo-homologue. Thus, a homology-based cloning approach could be more suitable than positional cloning for ol-2 isolation. 相似文献
57.
Transnasal administration of a combination of dexmedetomidine,midazolam and butorphanol produces deep sedation in New Zealand White rabbits
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Studies on the distribution of mammalian carnivores in fragmented landscapes have focused mainly on structural aspects such
as patch and landscape features; similarly, habitat connectivity is usually associated with landscape structure. The influence
of food resources on carnivore patch use and the important effect on habitat connectivity have been overlooked. The aim of
this study is to evaluate the relative importance of food resources on patch use patterns and to test if food availability
can overcome structural constraints on patch use. We carried out a patch-use survey of two carnivores: the beech marten (Martes foina) and the badger (Meles meles) in a sample of 39 woodland patches in a fragmented landscape in central Italy. We used the logistic model to investigate
the relative effects on carnivore distribution of patch, patch neighbourhood and landscape scale variables as well as the
relative abundance of food resources. Our results show how carnivore movements in fragmented landscapes are determined not
only by patch/landscape structure but also by the relative abundance of food resources. The important take-home message of
our research is that, within certain structural limits (e.g. within certain limits of patch isolation), by modifying the relative
amount of resources and their distribution, it is possible to increase suitability in smaller/relatively isolated patches.
Conversely, however, there are certain thresholds above which an increase in resources will not achieve high probability of
presence. Our findings have important and generalizable consequences for highly fragmented landscapes in areas where it may
not be possible to increase patch sizes and/or reduce isolation so, for instance, forest regimes that will increase resource
availability could be implemented.
Electronic supplementary material The online version of this article (doi:) contains supplementary material, which is available to authorized users. 相似文献
60.
Massimo Vignoli DR. MED. VET. SRV Stefanie Ohlerth DR. MED. VET. Federica Rossi DR. MED. VET. SRV Luigi Pozzi PROF. DR. MED. VET. Rossella Terragni DR. MED. VET. Daniele Corlazzoli DR. MED. VET. Barbara Kaser-Hotz PROF. DR. MED. Vet 《Veterinary radiology & ultrasound》2004,45(2):125-130
In humans, free-hand computed tomography (CT)-guided biopsy is an accurate method to obtain a tissue sample. There are only a few reports of this technique in veterinary medicine. In the present study, 21 dogs and two cats underwent a free-hand CT-guided tissue-core biopsy (17 animals) or fine-needle aspiration (six animals) of a bone lesion. Two out of 17 tissue-core samples were also cultured. All 17 tissue-core biopsy samples were diagnostic (accuracy of 100%). Five out of six aspirates were diagnostic (accuracy of 83.3%). The overall accuracy was 95.7%. In one aspirate, cytologic quality was insufficient containing only blood. No major complications were encountered. Fourteen neoplastic, two infectious and six benign lesions were diagnosed. CT examination after intravenous contrast medium added useful information to avoid large vessels and to biopsy-viable tissue. Free-hand CT-guided tissue-core biopsy and aspiration appears to be a safe and very accurate procedure for use in the diagnosis of bone-associated diseases in small animals. 相似文献