Mapping B-cell linear epitopes of NS3 protein of bovine viral diarrhea virus

Bovine viral diarrhea virus (BVDV) is a member of the genus Pestivirus within the family Flaviviridae. NS3 is one of the immunodominance regions of the BVDV viral proteins. To identify the potential B-cell linear antigenic epitopes within BVDV NS3 region, serial overlapping truncations covering the whole region were expressed and purified, and screened by multistep of Western-blot. We found (₁VCKKITEHERCHVNI₁₅), (₂₀AFFGVMPRGTTPRAPVR₃₆), (₄₆RRGLETGWAYTHQGGI₆₁), (₂₈₁EGDMATGITYASYGYFC₂₉₇), (₄₂₆YSGEDPANLRVVTSQSPYVVVATNAIESGV₄₅₅) and (₄₈₁FIVTGLKRMAVTVGEQA₄₉₇) can be recognized by the BVDV infected bovine serum. These proteins have been confirmed by indirect enzyme-linked immunosorbent assay (I-ELISA). The results of this study might open new perspectives on the structure and antibody–antigen reaction of the non-structural proteins and may aid in the clinical application as well.     

瘤胃缓释氮对奶牛泌乳性能的影响

试验旨在研究瘤胃缓释氮对奶牛泌乳性能的影响。选用胎次不同、产犊日期相近、泌乳中期的中国荷斯坦奶牛62头,随机分为两组(试验组和对照组),每组31头。试验前15d为预饲期。在预饲期内,试验组添加0.5%瘤胃缓释氮替代3%豆粕;到第16d添加1%瘤胃缓释氮替代6%豆粕;第38d添加1%瘤胃缓释氮替代6%的菜粕。结果表明,与对照组相比,试验组添加瘤胃缓释氮可提高奶牛对干物质的采食量,平均产奶量提高1.20kg,平均饲养成本降低0.33元/d,经济效益增加。     

青皮种子主要储藏条件的研究Ⅰ.种子含水量与测控

水分是影响青皮(Vatica astrotricha Hance)种子活力最关键的内在因素,其中种胚含水量是决定因素。用含水量27—29％椰糠控制种胚含水量在50—52％,也就是控制种子含水量在30—36％,维持种子一定的呼吸强度(32℃下测定为50—60 O_2μl/g·h),种子活力保持最高,能延长寿命。找出了储藏中种子安全含水量的最佳点,此点处于安全含水量下限的内缘,可利用种子本身果翅与种子或大小种子之间含水量的内在关系来测定。这种测定方法准确、快速又简便。可考虑作为确定“顽拗型”种子安全含水量下限的新捷径。     

酒泉市动物产地检疫现状及对策

随着社会经济迅速发展,酒泉市动物及动物产品流通日益频繁,动物产地检疫极为重要。本文主要论述了酒泉市动物产地检疫的作用、意义、现状、存在的问题及解决对策和措施。     

貂,貉,犬病毒性肠炎快速诊断试剂的研究

用金黄葡萄球菌Ａ蛋白（ＳＰＡ）协同凝集方法（ＣｏＡ）对貂、貉、犬病毒性肠炎快速诊断的试剂进行了制备，确定了制备。艺及该制剂的标准操作程序。本制剂特异性强，不与健康动物的组织及其他几种病毒病（犬瘟热、犬传染性肝炎、狂犬病等）发生交叉凝集反应，灵敏度高，通过该制剂ＣｏＡ法与ＨＡ－ＨＩ法比较结果比现行的貂、貉、犬病毒性肠炎ＨＡ－ＨＩ诊断方法检出率高出３０％，同时具有微量、操作简便、易掌握、快速等特点，可在３～５ｍｉｎ获得诊断结果。     

猪霉麦中毒和霉麦无害化处理的研究

对1983年嫩江地区某地猪喂霉麦引起的中毒进行了系统研究。通过霉菌菌相、菌量分析,明确了霉麦的优势菌为黄曲霉菌;通过流行病学调查、优势菌的产毒试验、毒素的化学确证试验、当日鸭雏的生物学确证试验以及猪的回归试验等,证明黄曲霉毒素B_1(AFT B_1)是造成猪短期内大批死亡的原因。同时,进行了霉麦无害化处理试验,初步证明用0.02％次氯酸钠处理具有杀菌、除毒效果可靠等特点。     

Equine chronic diarrhea: Traditional Chinese veterinary medicine review

Chronic diarrhea is a common problem in equine practice. Herbal medicine has been used for the treatment of gastrointestinal disease in horses for at least one thousand years. In present-day equine practice in China, the most common approach to treat chronic diarrhea is herbal medicine, or a combination of herbal medicine and acupuncture. Three types of chronic diarrhea in horses will be described in detail in term of symptoms, diagnosis and herbal treatments in this paper. Nineteen herbal formulas for treatment of different types of diarrhea will be introduced. Clinical results of each herbal formula will be evaluated. Acupuncture treatment of this problem will also be included.     

Studies on the pathogenicity ofBabesia bovis in water buffaloes after cryopreservation and resuscitation

Tropical Animal Health and Production - Packed erythrocytes infected withBabesia bovis were mixed with an equal volume of 16% dimethyl sulphoxide (DMS0) in Alsever’s solution and dispensed...     

Multiple amino acid substitutions are involved in the adaptation of H9N2 avian influenza virus to mice

To explore adaptation of avian influenza virus to mice we previously performed serial lung-to-lung passages of the influenza A/Chicken/Jiangsu/7/2002 (H9N2) strain, resulting in the isolation of a variant influenza strain lethal for mice. We now report that virulence correlates with improved growth characteristics on mammalian cells and extended tissue tropism in vivo. Sequencing of the complete genomes of the wild-type and mouse-adapted viruses revealed 25 amino acid substitutions. Some were found to reiterate known substitutions in human and swine H9N2 influenza isolates. Functions affected include nuclear localization signals and sites of protein and RNA interaction, while others are known determinants of pathogenicity and host specificity such as the viral polymerase PB2 E627K substitution. These observations suggest that enhanced growth characteristics and modified cell tropism may contribute to increased virulence in mice. We conclude that multiple amino acid substitutions are likely to be involved in the adaptation of H9N2 avian influenza virus to mice.