毛白杨85号高效遗传转化系统的建立

在建立以毛白杨85号( Populus tomentosa 85)叶片为外植体的再生系统基础上,从预培养时间、浸染菌液浓度、浸染时间、共培养时间及添加AS(乙酰丁香酮)5个方面,以卡那霉素抗性植株作为转化率的衡量指标,研究了各因素对毛白杨85号遗传转化率的影响,建立了高效的遗传转化系统。筛选的高效转化系统为：农杆菌活化菌液用MS液体培养基稀释10倍,浸染5min,共培养4d。毛白杨85号叶盘转化率可达40.0%。该研究为利用叶盘法开展毛白杨85号基因转化培育抗逆新品种奠定了良好基础。     

Does Dietary Administration of Lactococcus lactis Modulate the Gut Microbiota of Grouper,Epinephelus coioides

The effect of dietary administration of probiotic Lactococcus lactis MM1 for 60 d on the autochthonous microbiota in the foregut, midgut and hindgut of juvenile grouper, Epinephelus coioides, was evaluated using polymerase chain reaction‐denaturing gradient gel electrophoresis (DGGE). Samples collected from the probiotic and control groups showed different DGGE patterns, while 11 common bands presented in both groups. The similarity dendrogram revealed three different clusters depending on the three sections of the gastrointestinal (GI) tract, and the probiotic triplicates in each GI section were generally clustered into one group distinctly different from the corresponding control triplicates. Increased species richness and Shannon index were observed in the foregut, midgut, and hindgut samples in the probiotic group compared with the control group, which suggested that probiotic L. lactis MM1 could improve the autochthonous microbial diversity along the GI tract of E. coioides. The growth of many potentially beneficial and unidentified bacteria was stimulated by the probiotic, whereas the growth of some potential harmful species, like Staphylococcus saprophyticus, was depressed.     

新疆大蒜叶疫病的研究:Ⅱ病菌越冬致病性病害流行因素及防治

 试验结果表明,本菌(Stemphylium vesicarium(Wallr.) Simons)以病残体上分生孢子和菌丝体在田间和冷藏室越冬,9月至翌年6月检查,分生孢子萌发率为16.4~41.3%。人工接种试验指出,不论有无伤口,病菌均可侵染大蒜,但洋葱与大葱在伤口接种条件下病情较重。在自然条件下,大蒜的斑点型病状出现于6月下旬,7月为病情高峰期。影响病情的天气因素,主要是7月份温度和7月中旬的降水量(r=0.69)。如果7月雨次多、雨时长,病情即极为严重,可导致蒜田绝收。化学防治试验结果,以腐霉利防效最佳。从7月上旬开始,每间隔10~13天喷药1次。喷药2~3次即可取得较好的防治效果。     

荀子哲学关于心的看法及其对当代大学生思想政治教育的启示——从哲学关于他心及“性恶”的角度分析

先秦时期的诸子百家对于人心都有着自己独到的看法,从儒家的性善论到法家的性恶论,都体现着他们对于他人之心的揣度。而人心的善恶也直接关系着我们应该采取何种方式来对大学生进行有效地思想政治教育。这就需要我们细致研究关于心的哲学问题,从而有针对性地开展思想政治教育工作。     

宁夏六盘山不同森林类型土壤贮水与入渗研究

为了评价六盘山不同森林类型的水源涵养生态功能,本文对六盘山7种森林类型林地土壤的贮水和入渗特征进行了研究。结果表明:不同纯林林地的土壤饱和贮水量依次为5 151.867 t/hm3,4 798.500 t/hm3,4 632.533 t/hm3;不同混交林林地的土壤饱和贮水量依次为6 261.542 t/hm3,5 380.267 t/hm3,5 254.222 t/hm3,5 019.700 t/hm3;土壤贮水量随土壤容重和孔隙度变化的差异不显著,土壤持水率随土壤容重和孔隙度变化的差异极显著。不同纯林林地的土壤初渗速率依次为7.18mm/m in,9.06mm/m in,13.77mm/m in;稳渗速率依次为0.70mm/m in,2.04mm/m in,2.15mm/m in;不同混交林林地的土壤初渗速率为9.32mm/m in,8.20mm/m in,6.94mm/m in,3.38mm/m in;稳渗速率为2.74mm/m in,1.22mm/m in,2.50mm/m in,1.42mm/m in;土壤初渗速率和稳渗速率随土壤容重和总孔隙度变化的差异不显著,土壤初渗速率和稳渗速率随土壤毛管孔隙度与非毛管孔隙度变化差异极显著。     

Effect of dietary lysine on growth,intestinal enzymes activities and antioxidant status of sub-adult grass carp (Ctenopharyngodon idella)

The dietary lysine requirement of sub-adult grass carp (460 ± 1.5 g) was assessed by feeding diets supplemented with grade levels of lysine (6.6, 8.5, 10.8, 12.9, 15.0 and 16.7 g kg^?1 diet) for 56 days. The test diets (28 % CP) contained fish meal, casein and gelatin as sources of intact protein, supplemented with crystalline amino acids. Weight gain (WG), feed intake and feed efficiency were significantly improved with increasing levels of lysine up to 12.9 g kg^?1 diet and thereafter declined (P < 0.05). Quadratic regression analysis of WG at 95 % maximum response indicated lysine requirement was 10.9 g kg^?1 diet. Activities of trypsin, chymotrypsin, lipase, Na⁺, K⁺-ATPase and alkaline phosphatase in intestine, creatine kinase activity in proximal and mid-intestine responded similar to WG (P < 0.05). In addition, lipid and protein oxidation decreased with increasing levels of lysine up to certain values and increased thereafter (P < 0.05); the anti-hydroxyl radical capacity, dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione-S-transferase (GST) activities and glutathione content were increased with increasing dietary lysine levels up to certain values in the detected tissues, except for hepatopancreatic GST. Requirement estimated on the basis of malondialdehyde content in intestine and hepatopancreas was 10.6 and 9.53 g lysine kg^?1 diet, respectively.     

番茄裂果的原因及防治对策

番茄生产过程中,由于肥料的使用不当和水分灌溉多少直接影响番茄的裂果现象,针对出现番茄裂果,找出具体原因后对照症状,及时防治.     

籼稻Wx基因与稻米食用品质的相关性分析

以14个水稻品种及亲本为材料,通过检测各材料的Wx基因型,测定其直链淀粉含量(AC)、胶稠度(GC)和糊化温度(GT),对参试品种的Wx基因与AC、GC和GT的相关性进行了分析.结果表明,基因型为GG的品种具有较高的AC和较低的GC,而TT型品种具有中等的AC和较高的GC;AC与GC、GT呈极显著负相关,说明Wx除了控制稻米的AC之外,Wx与之紧密连锁的基因位点还可能影响GC的高低程度和GT的大小.对属于同一个恢复系的杂交组合的不育系和品种之间作偏相关性分析表明,优良品种的AC与不育系呈显著负相关,而品种的GC和GT与不育系之间的相关性却没有那么明显.     

劈破法扦插育苗试验研究

将仿照古代的劈破法与现今常用的马耳型剪法扦插育苗进行对比,证明劈破法扦插育苗有提高扦插成活率和促进多生根以及加快主梢高生长的效果,值得推广应用。