Isolation and Characterization of GhLEA3 Gene from Upland Cotton and Its Expression in Response to Low Temperature Stress

[Objective] Based on the analysis of cotton GhLEA3 gene structure and its expression model in chilling stress, we investigated the resistance function of GhLEA3 in response to cold stress. [Method] We cloned GhLEA3 from upland cotton by homologous sequence cloning way. We analyzed the properties of the GhLEA3, and constructed phylogenetic tree by bioinformatics analysis. We constructed the transient expression vector 35S∷GhLEA3-GFP by In-Fusion connection technology and studied the subcellular localization of GhLEA3. The expression of GhLEA3 gene in leaves of TM-1 at three-leaf stage under low temperature stress treatments (4 ℃, 24 h) was performed. Agrobacterium tumefaciens mediated floral dipping method was used to transform the gene with expression vector 35S∷GhLEA3-GFP into Arabidopsis thaliana wild type. Low temperature germination experiment at 4 ℃ was conducted to verify germination ability of the transgenic Arabidopsis thaliana T₃ seed. The transgenic Arabidopsis seedlings were treated at 4 ℃(low temperature), and all the leaves were taken to measure their electrical conductivity. [Result] The coding sequence of GhLEA3 gene is 1 218 bp, which encodes 405 amino acids. GhLEA3 protein includes 4 functional domains of PF02987. Phylogenetic analysis showed that the similarity of amino acid sequences between upland cotton GhLEA3 and Arabidopsis thaliana AtLEA3 was 52.6%. GhLEA3 might be mainly localized in vacuoles and small vesicles. GhLEA3 was up-regulated in leaves after low temperature treatment. The germination rate of transgenic Arabidopsis thaliana of T₃ generation at low temperature was significantly higher than that of wild type, and its electrical conductivity of leaves after low temperature treatment was significantly lower than that of wild type. [Conclusion] GhLEA3 belonged to the member of LEA3 family. Phylogenetic analysis showed that GhLEA3 had the closest relationship with AtLEA3. GhLEA3 was induced by low temperature stress and may play an important role in improving cold resistance.     

知识融合教学模式在“数据结构”教学中的应用

＂数据结构＂课程具有理论性和实践性强,以及与计算机专业其他课程关联密切的特点,可以将知识融合教学模式应用于该课程的教学中,从教学内容、实践环节和考核评价指标等方面进行改革。结果表明,采用知识融合教学模式能够显著提高教学质量,促进学生专业应用能力和综合素质的提高。     

生物有机肥在盐碱地上的应用效果及其对玉米的影响

通过筛选能够在高渗透压条件下生长发育的巨大芽孢杆菌和枯草芽孢杆菌,将其作为功能微生物发酵生产出生物有机肥,应用于盐碱地改良。同化学改良法比较得出,在投入相同的条件下,生物有机肥改良的盐碱地土壤理化性状显著改善,土壤微生物数量明显增多,生物量碳、土壤的呼吸作用和酶活性也有一定增加;玉米长势良好,植株健壮,叶色浓绿,未出现盐碱化症状,增产幅度明显。试验表明,利用生物有机肥进行盐碱地改良是一种经济有效的方法。     

山区丘陵地带土壤有机质插值方法的研究——以安徽省东至县为例

以安徽省东至县为例,系统研究了反距离加权插值法、径向基插值法、全局多项式插值法、局部多项式插值法、普通克里格插值法、简单克里格插值法、泛克里格插值法和析取克里格插值法对区域土壤有机质空间变异评价准确性的影响。结果表明,普通克里格插值法优于其他插值方法,所以东至县土壤有机质的插值分析应使用普通克里格插值法。     

加强农业科研档案管理 提高农业科研管理水平

文章从分析农业科研档案管理工作的重要作用入手,指出了目前我国农业科研院所在科研档案管理中存在的问题与不足,在借鉴国内外科研档案管理工作先进经验的基础上,提出了更新思想观念,提高科研档案管理意识;落实和完善科技档案规章制度,依法抓好档案管理工作;创新工作方式,提高工作效率;抓住项目重要节点,加强科研档案收集;加强人才培养,提高业务素质等对策。     

欧美杨107杨制浆造纸性能的径向变异

逐生长轮对107杨木材进行硫酸盐法化学制浆,并制备手抄纸片测定纸张力学性能,进行制浆造纸性能径向变异的研究.结果表明:107杨制浆造纸性能均值分别为制浆得率48.39％,卡伯值22.16,抗张指数85.61N·m·g-1,耐破指数58.38 kPa· m2 · g-1,撕裂指数13.72mN·m2 ·g-1.制浆造纸性...     

坡位和坡向对长白落叶松纸浆材材性的影响

为研究不同坡位、坡向对长白落叶松（Larix olgensis）纸浆材材性的影响,在东北林业大学帽儿山实验林场,于不同坡向和坡位设置样地并采集木材试样,对木材材性指标测定结果表明:坡向、坡位对长白落叶松基本密度、晚材率、纤维长和纤维宽影响不显著.试样纤维长宽比在不同坡位间差异不显著,但在不同坡向间差异显著,东坡和南坡明...     

高频引力波在电磁环形腔焦点区域的时空扰动效应

基于弱场条件,计算了在柱坐标下由电磁环形腔中强电磁简正模在其焦点区域产生的引力驻波的第二类克氏联络、里奇张量、曲率标量及其能量密度.研究发现,在焦点区域的引力驻波的能量密度是正定的.此外,讨论了该引力驻波的能量密度和时空的曲率标量的关系.高Q值强场环形微波腔以及受控核聚变的Tokamak装置均具有轴对称的特点,并可作为可能的潜在高频引力波源.     

水稻稻瘟病菌拮抗细菌的筛选与鉴定

采用平板测定法和盆栽试验结合筛选对稻瘟病菌具有高效抑菌活性的拮抗细菌,结果表明：从江苏省南京市、丰县、泗阳县等地的50份稻田水稻根际土壤样品中,得到1 015个细菌分离物,共获得45株对稻瘟病菌具有高效抑菌活性的拮抗细菌,其中10株对稻瘟病菌、水稻纹枯病菌和水稻稻曲病菌、水稻细菌性条斑病菌及水稻白叶枯病菌均表现出较强的广谱抗菌活性(对5种水稻病原菌平均抑制率在45％以上)。水稻苗瘟的盆栽防治效果表明：拮抗细菌T429和T392具有较好的防治效果,分别达到69.4％和61.1％。结合理化特性和16S rDNA鉴定,T429为枯草芽孢杆菌(Bacillus subtilis),T392隶属伯克霍尔德菌属(Burkholderia)。     

4种滨海造林树种耐盐性评价

基于室内盐胁迫处理试验,观察分析耐旱桑、果桑、胡杨、柽柳4个树种受盐害影响发生的生理变化,结果表明:叶片受盐害症状以耐旱桑表现最严重,其后从重到轻依次是果桑、胡杨、柽柳;随着盐胁迫质量分数的提高,光合作用受抑制加剧,耐旱桑和果桑受盐害影响较重且差异不显著,柽柳次之,胡杨受盐害影响最轻;叶绿素质量浓度随着盐胁迫质量分数增...