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排序方式: 共有349条查询结果,搜索用时 15 毫秒
71.
The prevalence of Middle East respiratory syndrome coronavirus (MERS‐CoV) antibodies in dromedary camels in Israel 下载免费PDF全文
Jennifer L. Harcourt Nir Rudoler Azaibi Tamin Eyal Leshem Michal Rasis Michael Giladi Lia M. Haynes 《Zoonoses and public health》2018,65(6):749-754
Middle East respiratory syndrome coronavirus, MERS‐CoV, was identified in Saudi Arabia in 2012, and as of January 29, 2018, there were 2,123 laboratory‐confirmed MERS‐CoV cases reported to WHO (WHO, 2018, https://www.who.int/emergencies/mers-cov/en/ ). Multiple studies suggest that dromedary camels are a source for human MERS‐CoV infection. MERS‐CoV‐specific antibodies have been detected in the serum of dromedary camels across Northern Africa and across the Arabian Peninsula. Israel's geographic location places Israel at risk for MERS‐CoV infection. To date, MERS‐CoV‐related illness has not been reported and the burden of MERS‐CoV infection in the Israeli population is unknown. The seroprevalence of MERS‐CoV‐specific antibodies in Israeli dromedary camels is unknown. The objective of this study was to determine the prevalence of MERS‐CoV seropositivity in dromedary camels in Israel. The prevalence of MERS‐CoV antibodies in Israeli camels was examined in 71 camel sera collected from four farms across Israel by MERS‐CoV‐specific microneutralization (Mnt) assay and confirmed by MERS‐CoV‐specific immunofluorescence assay (IFA). Although this study cannot rule out potential antibody cross‐reactivity by IFA, the presence of bovine coronavirus‐specific antibodies do not appear to impact detection of MERS‐CoV antibodies by Mnt. MERS‐CoV neutralizing antibodies were detectable in 51 (71.8%) camel sera, and no association was observed between the presence of neutralizing antibodies and camel age or gender. These findings extend the known range of MERS‐CoV circulation in Middle Eastern camels. The high rate of MERS‐CoV‐specific antibody seropositivity in dromedary camels in the absence of any reported human MERS cases suggests that there is still much to be learned about the dynamics of camel‐to‐human transmission of MERS‐CoV. 相似文献
72.
Nakamura S Tsuchiya H Okahara N Nakagawa T Ohara N Yamamoto H Li TC Takeda N Ogasawara K Torii R 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2012,74(3):279-283
A serological survey of hepatitis E virus (HEV) antibody was conducted using 202 adult captive cynomolgus monkeys, who did not show any clinical signs of acute hepatitis. Out of these, 44 monkeys were sero-positive for anti-HEV IgG and all monkeys were negative for anti-HEV IgM. All positive monkeys came from either Vietnam or China, but none from the Philippines, Indonesia, or our facility. Selected 12 monkeys out of positive monkeys from Vietnam, including 9 positive and 3 negative, revealed mostly within the reference ranges for alanine aminotransferase (ALT) and asparatate aminotransferase (AST) by serum biochemistries. Their titers of anti-HEV IgG did not correlate with the concentrations of ALT and AST. Moreover, HEV-RNA could not be detected from any fecal specimens of the 12 monkeys. Thus, monkeys with anti-HEV IgG sero-positive did not seem to be source of the HEV-pollution, because 1) sero-positive monkeys did not excrete HEV-RNA from their feces, and 2) monkeys from the Philippines and Indonesia have remained to be sero-negative for anti-HEV IgG, even if the monkeys were kept in same animal room of our facility. From these results, it could be inferred that primary infection of HEV occurred in the exported countries, but not in our colony. The contamination of HEV in indoor-captive monkeys could be prevented by precise quarantine tests, including ELISA for detecting anti-HEV and RT-PCR for HEV RNA. 相似文献
73.
Takeuchi Y Fujino Y Watanabe M Nakagawa T Ohno K Sasaki N Sugano S Tsujimoto H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2011,73(10):1295-1302
Options of systemic treatment for canine MCT have been still limited and most canine cases with MCTs eventually undergo relapses even after achievement of a remission. Thus additional therapies are required to establish for the tumor. To identify the novel candidate therapeutic targets for canine MCT, the mRNA expression and phosphorylation statuses of several receptor or non-receptor kinases as well as the inhibitory effect of 95 specific inhibitors on the growth were assessed in three canine MCT cell lines (HRMC, VIMC1 and CMMC1). Among the 14 targets, the mRNAs of 11, 7 and 7 kinases were amplified in HRMC, VIMC1 and CMMC1, respectively. The mRNAs of VEGFR3, PDGFRα, SRC, YES, LCK and FYN were detected in all cell lines. The phosphorylation of 12, 8 and 7 kinases was observed by using specific antibody arrays in HRMC, VIMC1 and CMMC1, respectively. DTK, EPHB6, AMPKα1, CREB, STAT5a and STAT5b were phosphorylated in all cell lines. The 10, 9 and 17 inhibitors exhibited the biological activity against the growth of HRMC, VIMC1 and CMMC1, respectively. Only three inhibitors such as SB218078 (for Chk1), PDGF RTK inhibitor IV (for PDGFR) and radicicol (for Hsp90) suppressed the growth of all three cell lines. The present study indicated that several kinases, such as Chk1, PDGFR and Hsp90, could be used as therapeutic targets in the treatment for canine MCT. Further studies and clinical trials are warranted to apply the inhibitors for the treatment of the tumor. 相似文献
74.
Kusuda S Nagami H Ueda K Nishikaku T Nakagawa D Takida T Kurita D Uemichi K Fukai M Kubota H Ueda K Ooe T Okuda K Kusunoki H Doi O 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2006,68(8):847-851
The purpose of present study was to determine annual changes in serum progesterone (P(4)) concentrations and to clarify basic reproductive characteristics, such as breeding season, estrous cycle, and puberty in female bharals (Pseudois nayaur). Blood was collected from 9 female bharals once or twice weekly for approximately one year. Serum P(4) concentrations were determined by radioimmunoassay. Serum P(4) concentrations showed remarkable and cyclic changes between November/December (winter) and May/June (late spring). The mean estrous cycle was 24.9 +/- 0.5 days. Chasing insistently to other females and discharge of mucus from the vulva were observed around the time when the serum P(4) concentrations began to increase. The chasing behavior and discharge of mucus were considered to be external indicators of estrus in female bharals. Serum P(4) concentrations of a pregnant female had non-cyclic changes, and the values remained high. In this study, all 37 deliveries were between April and September, and about 70% of these were concentrated in May and June. The conception month determined on the day of birth was between October and April for all animals, and the most common month was in December (54%). This month corresponded to an early stage of the period when the serum P(4) concentrations changed cyclically. These results indicate that many female bharals become pregnant at the beginning of the breeding seasons and, if they do not become pregnant, the estrous cycle, about 25 days in length, is repeated. 相似文献
75.
Antigenic relationships of Moraxella bovis isolates recovered from outbreaks of infectious bovine keratoconjunctivitis in Argentina, Brazil, and Uruguay between 1983 and 2000 下载免费PDF全文
Fabrício Rochedo Conceio Fernando Paolicchi Ana Lia Cobo Carlos Gil-Turnes 《Canadian journal of veterinary research》2003,67(4):315-318
Cross-reactivity indices (CRIs) of 28 isolates of Moraxella bovis recovered from outbreaks of infectious bovine keratoconjunctivitis in Argentina (A, 11 isolates), Brazil (B, 7), and Uruguay (U, 10) between 1983 and 2000 were estimated. Hyperimmune sera were produced in rabbits and antibody titres determined with each isolate. Isolates showing CRIs3 70 were placed in the same group. Group I had 13 isolates (A, 1; B, 6; U, 6); group II had 6 isolates (A, 4; U, 2); groups III, IV, and V had 2 isolates each, recovered in Argentina; group VI had 2 isolates, from Uruguay; and group VII had 1 isolate, from Brazil. The CRIs3 70 between vaccine strains and isolates recovered before and after 1990 were 58% and 42%, 50% and 50%, and 33% and 67% with vaccine strains 2419, 2358, and 2439, respectively. Isolate 273, from Uruguay, showed CRIs > 70 with 78% of the isolates and is recommended as the vaccine strain. 相似文献
76.
77.
Simone Saydelles da Rosa Nerinéia Dalfollo Ribeiro Evandro Jost Lia Rejane Silveira Reiniger Daniele Piano Rosa Taiguer Cerutti Micheli Thaise Della Flora Possobom 《Euphytica》2010,175(2):207-213
A large proportion of the world’s population is at risk of developing symptoms of zinc deficiency due to its low levels in
food. The objective of this research was to estimate the heritability in early hybrid generations for the zinc content character
in seeds of common bean (Phaseolus vulgaris L.), to investigate possible maternal effects on zinc contents and to evaluate the potential for genetic improvement. Reciprocal
F1, and F2 generations as well as backcross (RCP1 and RCP2) populations were produced by crosses of three cultivars. The seed zinc content was measured by the nitric-perchloric digestion
method and using the atomic absorption spectrophotometer. The range of variation in zinc content among tested progenies was
from 21.76 mg kg−1 dry matter to 53.48 mg kg−1 dry matter, and no significant maternal effect was discovered. Narrow sense heritability was of an intermediate 57.5% to
high 77.84% value and transgressive segregation was observed. These results suggest that breeding for increased zinc content
in the seeds of common bean can be quite successful; an increase of 37.3% was obtained using just the three parents tested
in this study. 相似文献
78.
Shaun Lisson Neil MacLeod Jeff Corfield Lalu Wirajaswadi Syamsu Bahar Nasruddin Razak Dahlanuddin Sania Saenong Lia Hadiawati Lisa Brennan 《Agricultural Systems》2010,103(7):486-497
Bali cattle (Bos javanicus) account for about one quarter of the total cattle population in Indonesia and are particularly important in the smallholder farming enterprises of the eastern islands. The population of Bali cattle is declining in most areas of Eastern Indonesia because demand for beef cattle exceeds the local capacity to supply these animals. Indonesian agencies recognise that new strategies are required to improve the productivity of Bali cattle and to address major constraints relating to animal husbandry and nutrition. To date, the adoption of cattle improvement technologies has been historically slow in Indonesia, as is the case elsewhere.This paper reports on key findings from a long-term study conducted between 2001 and 2009 with smallholder households from six villages in South Sulawesi and Central Lombok, to develop and test an approach for evaluating and increasing the adoption of cattle and forage improvement technologies. The approach is based on the principles of farming systems and participatory research and involved four main steps; (1) benchmarking the current farming system; (2) identifying constraints to cattle production and strategies to address them; (3) desktop modelling of the production and economic impacts of selected strategies; and (4) on-farm testing of the most promising strategies with 30 participant smallholder households.The approach was found to be successful based on: (1) sustained adoption of a package of best-bet technologies by the 30 participating households; (2) evidence of positive production, social and economic impacts; and (3) significant diffusion of the cattle improvement technologies to other households in the project regions. 相似文献
79.
Yoshiaki Nakagawa Guy Smagghe Soichi Kugimiya Kazunari Hattori Tamio Ueno Luc Tirry Toshio Fujita 《Pest management science》1999,55(9):909-918
Insecticidal activity against the Colorado potato beetle, Leptinotarsa decemlineata, was measured for a series of substituted N-tert-butyl-dibenzoylhydrazines, in which one of the benzoyl moieties closer to the tert-butyl group was fixed as being 2-chloro-substitued and the other variously substituted singly or doubly. The effects of substituents on the activity were quantitatively analysed using the classical quantitative structure–activity relationship (QSAR) procedure. The activity against the Colorado potato beetle increases with the molecular hydrophobicity. In addition, various types of steric effect are at work, depending upon the positions. Hydrogen-bonding acceptor substituents at the para position enhance the activity. There seem to be threshold (or optimum) values, albeit position-dependent, in the molecular hydrophobicity, above which the activity starts to decrease. This biphasic contribution of the molecular hydrophobicity to activity against coleopterous larvae is the most conspicuous difference in substituent effects from those found for similar compounds against lepidopterous pest insects, and may be the basis of the variations in the activity spectrum for certain compounds in this series. The introduction of bulkier substituents into the meta- and para-positions of the benzene ring, apart from the tert-butyl group, is unfavorable to activity. LD50 values against Colorado potato beetle larvae of methoxyfenozide (RH-2485) and tebufenozide (RH-5992) were in the order of 10−7 mol per insect, whereas those of RH-5849, and halofenozide (RH-0345) were very low, 10−9–10−10 mol per insect being selective to the coleopterous larvae. © 1999 Society of Chemical Industry 相似文献
80.
Rosa RM Melecchi MI da Costa Halmenschlager R Abad FC Simoni CR Caramão EB Henriques JA Saffi J de Paula Ramos AL 《Journal of agricultural and food chemistry》2006,54(19):7324-7330
The genus Hibiscus thrives in a variety of climates and produces a diversity of natural compounds with bioactive properties. We have studied the chemical composition and the in vivo antioxidant properties of Hibiscus tiliaceus L. methanolic flower extract, as well as its mutagenic/antimutagenic effects. Vitamin E and some stigmasterol derivatives that might confer an antioxidant effect to the extract were present. Treatment with this extract protected several Saccharomyces cerevisiae strains defective in antioxidant defenses against H2O2 and t-BOOH cytotoxicities, showing a clear antioxidant activity. The effect is the same for all strains used, independent of the antioxidant defense disrupted, suggesting that protection may be due to molecules that act as versatile and wide spectrum nonenzymatic antioxidants, such as vitamins or phytosterols. The extract was not mutagenic in either Salmonella typhimurium or S. cerevisiae and showed a significant antimutagenic action against oxidative mutagens in S. cerevisiae. 相似文献