水稻雄性不育突变体012S-3的遗传分析和基因定位

雄性不育是水稻杂种优势利用的重要资源,对雄性不育现象的研究具有重要理论意义和实践价值。本研究以自然突变的水稻雄性不育突变体012S-3为试验材料,对其表型特征和花粉育性等进行调查,并构建遗传群体,利用分子标记对目的基因进行初步定位,然后应用基因组重测序技术对其进行精细定位。结果表明,012S-3是一个典型的无花粉普通型雄性不育材料,其不育性状受1对隐性核基因控制。初步定位分析目的基因与SSR标记RM6081存在连锁关系,其遗传距离约为34.4 c M;进一步的精细定位分析,找到3个候选基因:LOC_Os07g35880、LOC_Os07g35920和LOC_Os07g35940,其中LOC_Os07g35880和LOC_Os07g35940编码β-淀粉酶,属于水稻中新发现的花粉致死基因。该不育基因的成功定位为其进一步的分离克隆及其在水稻分子设计育种中的应用奠定了基础。     

高温胁迫对无柄小叶榕光合作用的影响

以无柄小叶榕为材料,研究了不同时间高温处理对无柄小叶榕气体交换参数、叶绿素荧光参数和Rubisco活力的影响.结果表明,随着高温处理的持续,净光合速率(Pn)持续下降,而气孔导度(Gs)下降非常缓慢,蒸腾速率(Tr)则先开始快速上升随后保持稳定,但是胞间CO2浓度(Ci)却没有明显变化.进一步研究发现,在高温胁迫下,开放PSII反应中心激发能捕获效率(Fv''/Fm'')、光化学猝灭(gP)、PSⅡ实际量子产额(Φ<,PSⅡ>)和电子传递速率(ETR)都持续下降.Rubisco初始活力下降速度则快于这些荧光参数的变化.非光化学猝灭(NPQ)14是先迅速上升后来略有下降.这些结果表明高温抑制无柄小叶榕的光合作用不是由于气孔因素引起的,而是由于高温抑制了Rubisco活力,进而影响PSⅡ活力和电子传递.     

基于键合图理论的动力稳定装置轮轨间动态特性分析

考虑动力稳定装置的轮轨非线性接触、轮轨间的间隙传动、夹钳油缸和水平油缸的等效刚度建立了动力稳定装置动态特性键合图模型.使用分段函数表达式实现了轮轨间的间隙传动.针对不同夹持力对于能量的传递效率与作业效果进行了深入分析与探究,确定了所需最小的水平油缸的推力.     

动物骨粉的应用研究

动物骨头是肉类加工的副产品，由于富含蛋白质，钙、磷等微量元素以及多种维生素等营养成分，具有较高的营养价值，其对动物生产性能的促进作用早已被人们所认知，将其加工为骨粉后用于畜牧养殖已在生产实践中大量利用。了解骨粉的营养特性，掌握骨粉的加工及应用方法，将有助于充分发挥骨粉的营养功效，提高骨粉的利用价值。     

Field evaluation of insect-resistant transgenic Populus nigra trees

The performance of insect-resistant transgenic poplar trees (Populusnigra) expressing a Cry1Ac gene from Bacillus thuringiensis subsp. Kurstaki HD-1 against poplar defoliators was evaluated in the field at the Manas Forest Station in Xinjiang Uygur Autonomous Region during1994–1997. The results showed that the average percentage of highly damaged leaves on the transgenic trees was 10% while that on the control trees in nearby plantations reached 80–90%. The average number of pupae per m² of soil at 20cm depth in transgenic poplar plantation was 18 which was only 20% of that found in the non-transgenic control field. The number of pupae and the leaf-damage on transgenic trees described above are all far below the threshold set for chemical protection measures. The non-transformed poplar trees grown in the same plantation with the transgenic trees were also protected indicating that cross protection occurred between these two kinds of trees. Insect-resistant transgenic poplar trees have a potential application value in afforestation. This revised version was published online in August 2006 with corrections to the Cover Date.     

Effect of stachyose supplementation on growth performance, nutrient digestibility and caecal fermentation characteristics in broilers

1. The objective of this study was to evaluate whether the oligosaccharide stachyose enhances gastrointestinal tract health by fermentation and proliferation of desirable bacteria species and thus affects growth performance and nutrient digestibility in broilers. 2. A total of 432 1-d-old male Arbor Acres (AA) broilers were randomly allocated to one of 6 treatments, with 12 replicate pens per treatment and 6 birds per pen. Chicks were fed a maize-hamlet protein 300 (HP300) basal diet with 0, 4.0, 8.0, 12.0 or 16.0 g/kg stachyose. A sixth diet contained no HP300 but soybean meal (SBM) and provided 8.7 g/kg stachyose and 3.1 g/kg raffinose. The duration of the study was 42 d. 3. Stachyose contents above 12.0 g/kg depressed group body weights, average daily gain and feed/gain but not feed intake during the whole experimental period. Broiler growth decreased linearly and quadratically with increasing stachyose content. No differences were detected between diets supplemented with 12.0 g/kg stachyose and SBM. 4. Nutrient digestibility tended to decrease but not significantly with increasing stachyose. 5. Stachyose content had no significant positive effects on caecal pH, microflora population and the resulting short-chain fatty acid (SCFA) metabolites during the 42 d experiment, with only butyrate differing significantly in the initial period.     

高等农林院校数学建模教育研究与实践

结合1994～1998年甘肃农业大学大学生数学协会数学建模讨论班的实践经验,提出了：高等农林院校数学素质教育内容应包括数学基础教育、数学建模教育、数学建模实践3方面;数学建模教育的形式是“讨论班”;数学建模能力巩固、提高的方式是数学建模竞赛;要培养真正合格的素质型建设人才必须在大学期间参加适当的数学建模实践。并指出只有更多的专家、教授、学者参与到数学素质教育中来,使专业与数学教育真正相结合,数学建模教育乃至数学素质教育的口的才能真正实现。     

作物遥感估产的现状及其展望

 总结了国内外作物遥感估产的状况，并对作物遥感的技术集成和便利化服务、数据处理、农业标准化、农学机理、扩大用户需求共4个方面作了展望。     

山西省冬小麦生产潜力时空分布与气象因子分析

估算一定区域内作物生产潜力,对于明确该区域作物生产力及其限制因子具有重要意义。采用农业生态区域法和生产潜力递减机制法估算山西省冬小麦生产潜力,并解析其与主要气象因子的关系。结果表明,2004-2013年山西省冬小麦平均光合、光温、气候和土地生产潜力分别介于63.63~74.81、10.86~12.97、3.68~5.65和2.71~4.73t/hm ²。山西冬小麦生产潜力分布特征为南部普遍低于中部地区,中部盆地地区普遍低于东西部高海拔地区。与2004-2008年相比,2009-2013年晋中西部地区和吕梁冬小麦光合和光温生产潜力有所增加,而晋中东部和长治东部的冬小麦气候和土地生产潜力有所降低。限制冬小麦气候和土地生产潜力的主要气象因子是生育期降水量和日平均温度。