Accurate determination of 2,4,6-trichloroanisole in wines at low parts per trillion by solid-phase microextraction followed by GC-ECD

A headspace solid-phase microextraction (HS-SPME) procedure at 30 degrees C with a 100 microm PDMS fiber of a saturated NaCl solution stirred at 1100 rpm combined to GC-ECD for the 2,4,6-trichloroanisol (TCA) determination in wines has been developed. Due to the matrix complexity and ethanol absorption into the fiber, the internal standard selection was crucial to obtain unbiased results. Thus, matrix effects were observed when analyzing different types of Spanish wines (white, early, and vintage red wines) spiked with TCA at low concentration levels (i.e., <40 ng L(-)(1)). In contrast, the use of 2,4,6-tribromoanisole (TBA) as internal standard overcame these matrix effects, whereas the use of 2,4,6-trichlorophenyl ethyl ether led to inconsistent results. The developed HS-SPME-GC-ECD methodology reaches a limit of quantitation for TCA in wine within 2.9-18 ng L(-)(1), with a relative standard deviation of 2.5-13.4%, depending on the TCA concentration level and wine characteristics. This analytical method is comparable to the existing methodologies based on HS-SPME followed by GC-MS in terms of accuracy, precision, length of determination, and length of quantification; however, analysis cost is reduced.     

Assessing the Influence of Different Atmospheric and Soil Mercury Concentrations on Foliar Mercury Concentrations in a Controlled Environment

This study vividly presents results from a seasonal particulate matter measurement campaign conducted at world’s largest ship-breaking yard i.e., Alang-Sosiya (Gujarat, India) at six locations and a reference station at Gopnath which is 30 km south of this ship-breaking yard. The collected suspended particulate matter (SPM) 24-h samples were critically analyzed for heavy metals (Pb, Cd, Co, Ni, Cr, Mn, Fe, Cu, Zn). The average concentration of SPM within the ship-breaking yard during the investigation was 287.5 ± 20.4 μg m^?3 and at reference station it was 111.13 ± 5.81 μg m^?3. These values are found to be in excess of the permitted national standards. The levels of heavy metals at Alang-Sosiya are very high as compared to US EPA and WHO guidelines. The mean concentrations of all metals are in the order: Fe >>Zn >Cu > Mn > Cd >Pb > Co >Ni >Cr. The results on enrichment factors (EF) suggest that most of the metals in the ship-breaking yard exhibit EF values of near or above 100 which must have been comprehensively affected by ship-breaking activities. Metal data was used to evaluate the role of spatial factors on their distribution characteristics. Thereafter, factor analysis was carried out to identify the main components liable for the variance of the data set.     

Survey onYersinia Enterocolitica and related bacteria in shellfish

Results from examining shellfish collected from ‘safe’ (2–34Escherichia coli/100 mL) and ‘unsafe’ (> 34E. coli/100 mL) waters for the presence ofYersinia enterocolitica and related bacteria are reported. The recovery of these microorganisms was low in bivalve mollusks destined for food consumption, high in those derived from prohibited areas. No human pathogenic bio-serogroup f Yersinia was found during the entire survey.     

Integrated research in archaeology using soil micromorphology and palynology

Archaeologists often encounter problems in interpreting the genesis of layers and the context in which specific materials occur in excavations. Field visits of geologists, soil scientists or biologists can solve a number of questions, but often, problems remain that only can be studied by microscopic research as, for example, soil micromorphology and palynology. In the past, the employment of these two disciplines, one in earth science and one in biology, generally occurred independently of each other, also in interdisciplinary research and contract research. Consequently, results could not always be correlated, permitting only a restricted integration of data. It was realised that soils, as archives, contain much more information than generally utilised. A skillful employment and balancing of both specialties create a real integrated approach that offers a substantially improved interpretation potential without hardly additional efforts and costs. The results of 7 years of experience were very stimulating and presented here.In this article, the essence of soil micromorphology and palynology applied to archaeology is briefly outlined and illustrated by selected examples of the different research themes covered, showing the possibilities and results of integrated research. Both specialties generate essential information that, when combined and integrated properly, lead to improved understanding of the landscape genesis and human activities in the past. To be able to employ the full interpretive potential of this integrated approach, the research procedures followed by both specialties had to be compared. The key requirements for optimal integration of each other's databases comprised a combined effort to compare the pros and cons during the first four steps of the research procedure: defining research questions, field survey, selection of sampling locations and sampling. This approach enabled optimal correlation of the individual results, made cross-checking and references possible and led to higher levels of interpretation that would not have been possible otherwise. The integrated research elucidated lacunae in the other's discipline, resulting in a distinctly more complete reconstruction of paleoenvironments and past land uses, important research themes of archaeologists for the specialties concerned.     

Germline allele-specific expression of TGFBR1 confers an increased risk of colorectal cancer

Much of the genetic predisposition to colorectal cancer (CRC) in humans is unexplained. Studying a Caucasian-dominated population in the United States, we showed that germline allele-specific expression (ASE) of the gene encoding transforming growth factor-beta (TGF-beta) type I receptor, TGFBR1, is a quantitative trait that occurs in 10 to 20% of CRC patients and 1 to 3% of controls. ASE results in reduced expression of the gene, is dominantly inherited, segregates in families, and occurs in sporadic CRC cases. Although subtle, the reduction in constitutive TGFBR1 expression alters SMAD-mediated TGF-beta signaling. Two major TGFBR1 haplotypes are predominant among ASE cases, which suggests ancestral mutations, but causative germline changes have not been identified. Conservative estimates suggest that ASE confers a substantially increased risk of CRC (odds ratio, 8.7; 95% confidence interval, 2.6 to 29.1), but these estimates require confirmation and will probably show ethnic differences.     

For whose benefit?: A second look at fund raisers and other charitable responses to the U.S. farm crisis

The deepening U.S. farm crisis has been accompanied by numerous benefit fund raisers, individual donations and volunteer programs—all an expression of cooperation and concern on the part of U.S. citizens, farmer and non-farmer alike. These responses have received wide media attention and much public praise. A sense of patriotism and self-reliance underlies their popularity. Nevertheless, such efforts work to undermine their own ultimate objective—that of improving the economic circumstances of the family farm and farm family. This irony, it is argued, arises from the fact that these charitable responses typically depoliticize the nature of the U.S. farm crisis. First, they deflect public attention away from the larger economic context and the structural inequities within it. Second they revitalize agrarian-based myths which serve to rationalize and to reproduce the ‘independent’ behavior of small, commercial farmers. Finally, it is argued that the depoliticization of the farm crisis is itself a political strategy, one which supports and legitimizes ‘business as usual’ and is compatible with the interests of corporate agriculture.