禽白血病病毒J亚群诱导蛋鸡群多种肿瘤

2009年8月,山东泰安某海兰褐商品蛋鸡场,开产后产蛋率只有60％～70％,31周龄时,死亡率突然增加,最高达20％.患病鸡经大体剖检、病理组织学、PCR和免疫组织化学检测确诊为J亚群禽白血病.病理组织学检测发现送检鸡除了髓细胞瘤外,还存在明显的纤维肉瘤、组织肉瘤、腺癌和血管内皮瘤等肿瘤.纤维肉瘤单独存在,组织肉瘤、腺癌和血管内皮瘤分别和髓细胞瘤混合存在,本文详细描述了各肿瘤的组织学特点.ALV-J从肉种鸡传向商品蛋鸡后引起肿瘤谱扩展的原因和机制值得进一步研究.     

黄粉虫粪不同用量对制种黄瓜幼苗质量的影响

研究了营养土中不同黄粉虫粪搀入量对马坊营旱黄瓜幼苗生长的影响，结果表明：用黄粉虫粪与营养土按1：(160-320)(体积比)混配的基质进行黄瓜育苗，能促进幼苗生长，显著提高幼苗简单指标、相对指标和复合指标的值。     

松茸菌株对6种松树幼苗的感染及其菌根解剖学研究

利用我国滇产松茸9924菌株及日产松茸99606菌株，分别对云南松、华山松、马尾松、火炬松、湿地松及加勒比松6种松树幼苗实施人工菌根接种。研究结果表明，接种后90d的云南松和湿地松分别有75％和78．1％的幼苗受感染并形成菌根；接种后180d,6种松树幼苗分部都可形成菌根，菌根感染率分别达90．63％－100％，感染指数达50．00－84．68；菌根形态主要有棒状、二叉分枝状及多叉分枝状3种；随树种的不同，各类菌根形态所占比例也不同；不同树种根系上形成菌根的长度也有差异，以马尾松根系上的菌根最长，华山松的菌根最短；此外，各种组合之间在菌套厚度，哈蒂氏网结构上也略有差别。     

Expression of miRNA-22 in ovarian cancer and effect of miRNA-22 over-expression on SKOV-3 ovarian cancer cell proliferation,migration and invasion

AIM: To examine the expression of miRNA-22 in the ovarian tissues and the effect of miRNA-22 over-expression on the proliferation, migration and invasion in SKOV-3 cells. METHODS: The expression levels of miRNA-22 in different ovarian tissues and SKOV-3 cells were determined by qPCR. miRNA-22 was over-expressed by transfection of miRNA-22 mimic. The cell viability was examined by CCK-8 assay. The cell migration was measured by wound healing test. The cell invasion was analyzed by Transwell assay. The protein expression levels of VEGF and P53 were determined by Western blot. RESULTS: Compared with the normal ovarian tissue, the expression level of miRNA-22 was remarkably decreased in the ovarian tumor tissues. After transfection with miRNA-22 mimic, the expression level of miRNA-22 in the SKOV-3 cells was significantly increased, while the cell viability, migration and invasion were obviously decreased. Moreover, the protein expression of VEGF and P53 was dramatically inhibited after over-expression of miRNA-22. CONCLUSION: The decreased miRNA-22 expression may be correlated with the development of ovarian can-cer. Over-expression of miRNA-22 decreases the cell viability, migration and invasion by reducing the protein expression of VEGF and P53.     

中国主要纸产品国际竞争力综合评价

文中在分析中国主要纸产品出口贸易现状的基础上,选取国际市场占有率、贸易竞争力指数和显示性比较优势指数测算中国主要纸产品2010-2019年的国际竞争力指数,并采用基于主成分分析的定权方法对纸产品国际市场竞争力进行综合评价。结果表明:主要纸产品国际竞争力较强的芬兰、瑞典和德国位居综合评价前3位,中国主要纸产品综合竞争力经过近10年发展已处于中等偏上水平,仍有改进和提升空间。基于上述研究成果,提出推进人工林速生材高效利用、促进科技创新能力提升和探索"出口转内销"等政策建议。     

链格孢菌（Alternaria tenuissima）cDNA酵母表达文库的构建

为了解链格孢菌（Alternaria tenuissima）遗传学背景,克隆及研究该菌功能基因,根据Gateway技术构建了既能在酵母细胞中表达外源基因又能在原核细胞大量复制的酵母表达载体pRS-DEST42,构建了细极链格孢菌（A. tenuissima）cDNA酵母表达文库。经检测,该文库的平均滴度为2.44×106（cfu/ml）,文库总容量为2.44×107,阳性克性率为100%,平均插入片段约为1.38kb。细极链格孢菌（A. tenuissima）cDNA酵母表达文库是一个质量较高的表达文库,为克隆与分离全长目的基因及其功能奠定了坚实的基础。     

新疆克拉玛依市兴农湖剖面古沉积环境分析

粒度是沉积物中各种粗细颗粒的机械组成,可以用于沉积环境类型的识别和物质运动方式的判定。通过对克拉玛依市兴农湖剖面的粒度数据测量分析,判定该地区沉积物为湖相沉积。联系周围区域构造形态,该地区应为古玛纳斯湖的湖盆地区。     

Low levels of haptoglobin and putative amino acid sequence in Taiwanese Lanyu miniature pigs

Porcine haptoglobin (Hp) is an acute phase protein. Its plasma level increases significantly during inflammation and infection. One of the main functions of Hp is to bind free hemoglobin (Hb) and inhibit its oxidative activity. In the present report, we studied the Hp phenotype of Taiwanese Lanyu miniature pigs (TLY minipigs; n=43) and found their Hp structure to be a homodimer (beta-alpha-alpha-beta) similar to human Hp 1-1. Interestingly, Western blot and high performance liquid chromatographic (HPLC) analysis showed that 25% of the TLY minipigs possessed low or no plasma Hp level (<0.05 mg/ml). The Hp cDNA of these TLY minipigs was then cloned, and the translated amino acid sequence was analyzed. No sequences were found to be deficient; they showed a 99.7% identity with domestic pigs (NP_999165). The mean overall Hp level of the TLY minipigs (0.21 +/- 0.25 mg/ml; n=43) determined by enzyme-linked immunosorbent assay (ELISA) was markedly lower than that of domestic pigs (0.78 +/- 0.45 mg/ml; p<0.001), while 25% of the TLY minipigs had an Hp level that was extremely low (<0.05 mg/ml). In addition, the initial recovery rate (first 40 min) in the circulation of infused fluorescein isothiocyanate (FITC)-Hb was significantly higher in the TLY minipigs with extremely low Hp levels than those with high levels. This data suggests that the low concentration of Hp-Hb complex is responsible for the higher recovery rate of Hb in the circulation. TLY minipigs have been used as an experimental model for cardiovascular diseases; whether they can be used as a model for inflammatory diseases, with Hp as a marker, remains a topic of interest. However, since the Hp level varies significantly among individual TLY minipigs, it is necessary to prescreen the Hp levels of the animals to minimize variation in the experimental baseline. The present study may provide a reference value for future use of the TLY minipig as an animal model for inflammation-associated diseases.     

不同化学激活方法和电激活参数对猪体外成熟卵母细胞早期孤雌发育的影响

利用屠宰场猪卵巢卵母细胞,在体外成熟培养44～48 h后,对核成熟卵母细胞进行激活.试验1为不同化学激活方法10% 乙醇 10 mg/L 放线菌酮组、2.5 mmol/L 氯化锶 10 mg/L放线菌酮组、5 μmol/L离子霉素 2.5 mmol/L 6-二甲氨基嘌呤(6-DMAP)组、200 μmol/L 硫柳汞 8 mmol/L二硫苏糖醇组和对照组(不用任何激活剂).试验2为用不同电场强度和脉冲时程进行电激活之后放入胚胎培养液中进行体外培养7 d.结果显示,(1)4种不同化学激活方法处理卵子的1原核形成率、2原核形成率和原核形成率都显著比对照组高(P＜0.05),其中离子霉素 6-DMAP组的2原核形成率和总原核形成率最高,分别为(23.1±3.5)%和(65.2±3.5)%,显著高于其他处理组(P＜0.05);(2)4种不同化学激活方法处理组的囊胚率都比对照组高(P＜0.01),其中离子霉素 6-DMAP组的卵裂率及囊胚率最高,分别为(46.6±18.5)% 和(5.6±4.2)%;(3)本试验所用的4种不同化学激活方法对猪4-细胞孤雌胚SDS-PAGE电泳的蛋白质表达图谱没有显著影响;(4)电场强度为1.7 kV/cm、脉冲时程为50、70 μs时猪体外成熟卵母细胞激活效果最好,卵裂率、囊胚率、囊胚细胞数分别为(77.4±9.7)%、(12.4±3.7)%、(17.6±5.9)%和(75.1±10.6)%、(12.3±2.6)%、(19.1±8.1).以上结果说明本试验所用的4种化学激活方法均能有效激活猪体外成熟卵母细胞,其中离子霉素 6-DMAP的激活效果最理想;在本实验室条件下,采用1.7 kV/cm的电场强度、50～70 μs的脉冲时程均能有效地激活猪体外成熟卵母细胞;本试验所用的4种不同化学激活方法对猪4-细胞孤雌胚SDS-PAGE电泳的蛋白质表达图谱没有显著影响.     

提高柠条饲料利用率的研究

柠条Caragana korshinskii粗纤维高、适口性差、利用率低,采用物理、生物、化学处理技术进行加工处理.结果表明:柠条经过粉碎制粒后饲喂家畜,利用率提高50%,家畜采食量增加20%～30%,质量增加15%左右;柠条添加5%玉米进行氨化处理,效果好,粗蛋白含量提高6.32%,粗纤维含量降低3.67%,木质素含量降低1.32%,无氮浸出物含量降低1.49%;柠条青贮后粗蛋白含量降低0.06%,粗纤维含量降低1.6%,木质素含量降低2.68%,无氮浸出物含量降低1.87%;添加5%尿素膨化处理,效果好,柠条粗蛋白含量提高10.53%,粗纤维含量降低1.37%,木质素含量降低3.03%,无氮浸出物含量降低8.69%;柠条微贮后,粗蛋白、粗脂肪、粗纤维、木质素含量变化不大,粗灰分含量增加1.36%,无氮浸出物含量增加2.59%.