适用于转录组测序的大白菜小孢子总RNA提取方法筛选

为了找到适用于转录组测序要求的大白菜小孢子总RNA 提取方法，以出胚率较高的吉红82 为试材，以热激诱导处理后的小孢子为研究对象，比较分析了TRIzol 法、改良CTAB 法及超纯试剂盒法3 种方法的RNA 提取效果。结果表明：TRIzol法（磨样）和改良CTAB 法提取的RNA 浓度较高，但完整性较差；超纯RNA 提取试剂盒法提取的RNA 质量最高，且完整性好，能够满足转录组测序的要求。     

Effect of miR-19a on lipid catabolism in hepatocyte LO2

AIM: To observe the effect of microRNA-19a (miR-19a) on the lipid catabolism of hepatocyte LO2, and to explore the potential mechanism. METHODS: miR-19a was over-expressed or silenced by transfection of miR-19a mimics or miR-19a inhibitor into LO2 cells, then the mRNA level of miR-19a was detected by real-time PCR. The potential target of miR-19a was found by the method of bioinformatics through internet website. The effect of miR-19a on the 3' UTR of peroxisome proliferator-activated receptor α (PPARα) was measured by dual luciferase reporter assay, and the protein level of PPARα and its 2 major downstream rate-limiting enzymes involved in lipid catabolism, acyl-coenzyme a dehydrogenase (ACADM) and carnitine palmitoyltransferase 1A (CPT1A), were detected by Western blotting. Meanwhile, the effect of miR-19a on the generation of ketone body was measured by beta-hydroxybutyric acid (β-OHB) detection assay. RESULTS: The mRNA level of miR-19a was dramatically elevated by the transfection of miR-19a mimics, and sharply decreased by the transfection of miR-19a inhibitor (P<0.05). PPARα was found as a potential target of miR-19a, and dual luciferase reporter assay and Western blotting confirmed the regulatory effect of miR-19a on the expression of PPARα, with the protein level changes of ACADM and CPT1A. miR-19a mimics down-regulated, while miR-19a inhibitor up-regulated the concentration of β-OHB in LO2 cells (P<0.05). CONCLUSION: miR-19a regulates the lipid catabolism of hepatocytes by targeting the PPARα and its 2 downstream rate-limiting enzymes.     

大白菜花青素含量及色差指标相关性研究

为了探寻大白菜花青素含量快速测定的方法,本文利用pH差计法和色差仪分别测定了‘城阳青’(普通大白菜)、‘秋宝黄’(黄心白菜)和‘彩凤’3个不同叶色大白菜品种花青素含量以及色差指标,并对6个色泽参数与花青素含量进行了相关和回归分析。结果表明:6个色泽参数均能反映白菜叶片间叶色的细微变化,‘城阳青’和‘秋宝黄’不含花青素,紫色白菜‘彩凤’的花青素主要分布于由外向内的第1～4层叶片。色泽参数L,b,h和C分别与花青素含量呈极显著或显著负相关关系(相关系数分别为-0.85**,-0.58**,-0.49*,-0.58**)。以色泽参数值L、a、h与花青素含量建立了多元回归方程:y=90.24-1.07x1+5.65x2-142.71x3(x1为L值,x2为a值,x3为h值,y为花青素含量),该回归方程相关系数为0.9629。通过色差计依据建立的回归方程可快速测定紫色大白菜花青素的含量。     

文化创意产业与旅游业的融合模式研究--以五大连池风景区为例

文章从文化创意产业与旅游业的融合角度着手,以五大连池风景区为例,通过分析五大连池风景区火山与矿泉文化资源,进一步从文化创意与旅游融合的角度提出拓展型融合、渗入型融合、重装型融合的五大连池火山矿泉疗养文化与旅游业的融合模式。     

一株新疆灵芝的鉴定及生长和产糖性能初探

以新疆阿勒泰采集的灵芝子实体XJ-001为试材,采用组织分离纯化获得该菌纯培养,以菌丝体基因组DNA为模板进行ITS片段扩增,测序并进行系统发育分析;以5种不同培养基培养菌株,通过比较生长速度,选出适宜母种培养基;摇瓶发酵研究不同碳氮源对菌株菌丝体生物量、胞内及胞外多糖含量的影响。结果表明:根据形态特征结合rDNA-ITS分析,初步将XJ-001鉴定为Ganoderma lucidum,并获得GenBank登录号为KT997431;菌株在玉米粉、豆饼粉琼脂培养基长势最优,生长速度为18.7mm·d~(-1);以葡萄糖、酵母浸粉为最适碳、氮源,在转速150r·min~(-1),30℃培养5d,菌株XJ-001菌丝体生物量、胞内/外多糖含量分别为8.15、1.18、0.05mg·mL~(-1)。该菌株具有生长迅速和产糖较高的潜在性能,可为开发新疆野生灵芝资源提供参考。     

不同行道树降温增湿及滞尘效应

以海南热带海洋学院(三亚校区)校园行道树优势树种高山榕、大叶相思、印度胶树、菩提树、非洲楝、火焰木、马占相思、香樟、鸭脚木、小叶榄仁、垂叶榕、隆缘桉为研究对象,比较了乔木绿化树种的降温增湿和滞尘能力。结果表明:在相同环境生长的种龄相当的树种,降温增湿效应不同,高山榕、大叶相思、印度胶树的降温增湿能力最好;降温增湿效应比较差的是隆缘桉、小叶榄仁和垂叶榕。不同树种的滞尘能力也有差异,其中滞尘能力较强的乔木树种是火焰木、菩提树、高山榕和印度胶树。该研究结果对校园绿化和城市行道树的规划选择都具有一定的参考价值。     

On Noise Control of the Environment in Advanced Living District

The living environment is becoming more and more important,one of the main elements which affect it is noises.This paper takes the Zhonghuafang for example,to research how the whole planning and the designing for the living district affect the control of noises.Applying architectural technology,in order to cut down noises,a sound screen around the site is made and the distinctive constitution for the buildings is designed.The methods of disposing noises are proposed to make sure that the residents of the advanced living district are content with the living environment.     

Study and Practice of Safety Grade Assessment for Large Convention and Exhibition Centre

This paper takes "how to estimate the complex security of Large Convention and Exhibition Centre effectively" as the research goal.Based on analyzing the difficulties,target system,technical constitution,probing into the architecture,safety science,and using for reference the mathematics,operational research,statistics and modern system theory and method,trying to explore establishing an evaluation methodological system,which is provided with integrated characters on subject.Based on these ways,it exerts "quality to quantity" fuzzy analytical hierarchy process(FAHP) and aims at discussing the safety grade assessment of the Chongqing International Conference Exhibition Center focusing on designing theories.The aim is to enhance the integration of design practice and safe consciousness,strengthen the building safety management,and perfect city public security system.In practice,it is also an efficient tool for favoring values of architectural environmental safety.     

黑龙江省农业经济增长方式转变的实证分析

在分析农业经济增长理论的基础上，运用算术指数法得到黑龙江省农业的全要素生产率。依据全要素生产率对黑龙江省农业改革以来的增长方式做了定量描述，从实证分析的角度，考察各种要素投入的增长及其对农业经济增长的贡献，衡量改革以来科技进步在黑龙江省农业生产中的状况和作用，进而描述黑龙江省农业经济增长方式的演进过程，为黑龙江省确定今后加快实现农业经济增长方式转变的政策和战略提供参考依据。