20个月季品种的抗寒性综合评价

以兰州植物园的20个月季品种一年生枝条为供试材料,测定了低温胁迫下的耐寒指标,利用灰色关联分析法得出各指标对抗寒性评价的影响程度,以各项指标的抗寒系数作为评价月季抗寒能力的指标,通过主成分分析法、隶属函数综合评价和聚类分析,将20个月季品种按抗寒性强弱划分成了5类,并进行了抗寒能力评价.结果表明:(1)低温胁迫下供试月季的枝条存活率(SR)和发芽率(GR)较对照平均下降了52.27％ 和33.57％,可溶性糖含量(SS)和可溶性蛋白含量(SP)较对照平均下降了54.73％ 和87.40％,相对电导率(RC)较对照平均上升了127.00％,丙二醛含量(MDA)变化规律不一致.(2)各指标中MDA含量对抗寒性评价的影响最大,其次为枝条发芽率和存活率,可溶性糖含量的影响最小.(3)从供试20个月季品种中筛选出抗寒性最强的为'香魔'香妃'百老汇',其次为'赞歌'阿尔卑斯'和'电子表',这些品种适宜在寒冷地区引种栽培.     

Cloning and Bioinformatic Analysis of L1 Gene of BPV from Guizhou Province

In order to study and analyze L1 gene of bovine papillomavirus（BPV）in Guizhou province,the L1 gene of BPV-GZ01 strain was amplified,cloned and sequenced using bioinformatic softwares and methods,and the secondary structure,tertiary structure,B-cell preponderant epitope,conserved domains analysis, transmembrane domain and signal peptide of L1 gene were predicted.The results showed that the length of L1 gene was 1 494 bp,encoding 497 amino acids.The L1 gene of BPV-GZ01 strain shared an amino acid identities of 98.6%,99.4%,98.4%,94.4% and 91.3%,and a nucleotide identities of 99.1%,99.8%,99.4%,87.6% and 82.8% with those of BPV2,BPV2-SW01,BPV2-AKS01,BPV13 and BPV1 strains,respectively.The results of phylogenetic tree analysis indicated that there was a close relationship between BPV-GZ01 and BPV2-SW01 strains.The prediction of secondary structure of L1 protein indicated that the random coil,extended strand and alphahelix took a higher percentage.The L1 protein was supposed contain 6 potential antigen epitopes.And no transmembrane domains and no signal peptide were found.The tertiary structure of L1 protein was curved spiral structure.These results provided a theoretical basis for immunologic diagnosis and further research of nucleic acid vaccine of BPV.     

褪黑素的生物合成及对繁殖性能的影响

褪黑素是一种广泛存在于动物体内的激素,不仅能够调节动物昼夜节律,还能够从多方面影响动物的繁殖性能,对于季节性繁殖动物,褪黑素根据日照时长的变化表现出抑制或促进繁殖性能的作用。对于幼年动物,褪黑素表现出抑制发情的作用,体成熟后则表现促进发情的作用。褪黑素主要通过下丘脑-垂体-性腺轴调控动物繁殖活动,除此之外,褪黑素还能够直接作用于生殖系统甚至早期胚胎,通过提高组织及细胞抗炎及抗氧化功能来改善动物繁殖性能。目前,褪黑素能够用于治疗精神疾病及炎症、促进畜禽繁殖和调节昼夜节律,是相关研究领域的热门话题。本文详细描述了褪黑素的生物合成以及分泌调控,并结合褪黑素的生理功能,阐述了对动物繁殖性能以及辅助生殖的影响。     

牛奶中褪黑素含量的检测方法

褪黑素作为一种多功能活性因子,在畜牧生产中具有十分重要的作用。近年来,有关天然高褪黑素奶的研究大规模开展,对奶中褪黑素含量测定需求也有所增加。目前比较常见的褪黑素检测方法有气相色谱法（GC）、气相色谱-质谱联用法（GC-MS）、高效液相色谱法（HPLC）、高效液相色谱-质谱联用法（LC-MS）、放射免疫测定法（RIA）、酶联免疫吸附测定法（ELISA）、紫外分光光度法（UV）。本文从原理、特点及优缺点等方面对这几种方法展开介绍,并对各方法的应用进行简单的比较,为后续相关研究与应用提供一定参考。     

突发性环境污染事故应急监测的速度保障

针对突发性环境事故如何保证应急监测速度的问题，从应急监测前准备工作及应急监测运行期两方面进行说明，为保障应急监测速度提供了切实可行的方法和步骤。     

Automatic Measuring Method of Eyeglass Lens Power

A method of measuring eyeglass lens power is proposed. A position detector and 3 beams of ray are used to measure the prismatic, spherical and astigmatic powers. Based on the method, an automatic focimeter with simple structure can be developed. The measure errors of prismatic and spherical powers are analyzed, from which it is concluded that the precision of the position detector is the key to the method.     

Experimental Study of Multifunctional Acid to Deliquesce Stratum-jam

Aiming at the oneness of acid to deliquesce stratum-jam applied in scene at the present time,which is incapable of deliquescing complex dirty simultaneously,this article confects a new multifunctional O/W acid.This acid is an emulsified mixture containing 15% hydrochloric acid as its extro-posture,high dissolved oil as its internal state under the function of some proper exterior active reagent.It is composed of 0.5%OP-10,0.5%Span80,0.2%Tween80,0.06%imidazole and 0.01%twelve-alcohol,and the content of oil is equal to the acid content.It can be indicated that this acid has good ability of resolving mineral(CaCO_3,MgCO_3) and organic compound(mainly colloidal bitumen) when it was poured into the stratum,so that it can deliquesce oleic stratum-jam,resume and improve the penetrability of stratum,boost the ability of liquid flowing up.     

Dropwise Condensation Heat Transfer Coefficient on the Horizontal Circular Surface with Radial Gradient Surface Energy

A theoretical model is developed for the dropwise condensation heat transfer on the horizontal circular surface with radial gradient surface energy based on the heat transfer model of individual condensate drop and the size distribution model of condensate drop on homogeneous condensation surface.The effect of variation of contact angle on the gradient surface on condensation heat transfer and condensate drop size distribution is taken account of in this model.The theoretical calculation method was obtained to predict the dropwise condensation heat transfer coefficient on the horizontal circular surface with radial gradient surface energy under various wall subcooled temperature,contact angle profile on wall surface,and working fluid.The effects of surface energy gradient,wall subcooled temperature,and thermophysical properties of condensate on the condensation heat transfer are discussed respectively.The calculation results show that the condensation heat transfer coefficient increases slightly with the increase of wall subcooled temperature.As latent heat and surface tension increasing,the condensation heat transfer coefficient increases.A larger surface energy gradient induces a larger condensation heat transfer coefficient.