纳米TiO2悬浮液对烟草青枯菌杀菌活性的研究

纳米T iO2是一种半导体光催化剂,在近紫外光的激发下,可以产生强的杀菌活性。以烟草青枯菌P seudomonas solanacearum为试验对象,采用平板稀释法对自制的纳米T iO2悬浮液进行杀菌活性测定。结果表明:在阳光或汞灯下照射4h后,T iO2对烟草青枯菌的杀菌率可达90%以上。说明用纳米T iO2防治烟草青枯病具有一定的可行性。     

Proliferation-inhibitory and apoptosis-inducing effects of cinnamic acid combined with cisplatin on human hepatocellular carcinoma cell line MHCC97

AIM：To investigate the effects of cinnamic acid (CA) combined with cisplatin on the proliferation and apoptosis of human hepatocellular carcinoma cell line MHCC97. METHODS：Human hepatocellular carcinoma cell line MHCC97 was culured and divided into CA group, cisplatin group, CA+cisplatin group and control group. MTT assay, inverted microscopy, annexin V-FITC/PI staining and flow cytometry were applied to identify the viability, morphology and apoptosis of the cells. The apoptosis-related signaling protein caspase-3 was detected by Western blotting. RESULTS：CA and cisplatin either alone or in combination significantly inhibited the proliferation and induced obvious apoptosis of MHCC97 cells, while CA alone or combined with cisplatin had no significant inhibitory effect on normal human liver L-02 cells. The rates of mid-and late apoptosis or necrosis were higher in cisplatin group than that in CA group or combination group, but the early apoptotic rate was just the opposite. Pro-apoptotic activity in combination group was much stronger than that in CA group or cisplatin group at lower concentration, and combination group promoted apoptosis and decreased the cytotoxic side effects of cisplatin. CA and cisplatin either alone or in combination also up-regulated the cleaved caspase-3 expression in a time-dependent manner, and the effects in CA group and combination group were higher than that in cisplatin group. CONCLUSION：CA and cisplatin either alone or in combination inhibit the growth of MHCC97 cells by inducing apoptosis, and the activation of caspase-3 may play important roles in these processes.     

塔里木河干流中游胡杨种群特征与动态分析

通过样地调查和数据统计,编制胡杨种群静态生命表,绘制存活曲线、生存率曲线、累计死亡率曲线、种群死亡密度曲线和危险率曲线,分析种群数量特征。同时,结合种群动态量化方法及时间序列预测,分析种群数量动态变化。结果表明:胡杨种群年龄结构呈金字塔型,幼龄级所占比例较大;存活曲线接近Deevey C(凹)型,且动态指数大于0,表明胡杨种群结构合理,为增长型种群。4个函数曲线表明,胡杨种群前期幅度变化较大,后期趋于稳定。时间序列分析表明,由于丰富的后续资源,种群能够稳定生长。     

Comparison Analysis of Immune Related Gene of Orf Virus in Sheep and Camels

The purpose of this study was to investigate the variation of Orf virus (ORFV) immune related genes after infection with different species.The ORFV genomes of sheep and camel were extracted and named ORFV-Y and ORFV-LT,respectively.Based on ORFV genome sequence published in GenBank (accession No.:KF234407.1),three pairs of specific primers were designed and synthesized to amplify the B2L,F1L and VIR gene fragments of ORFV-Y and ORFV-LT,respectively,and the amplified fragments were cloned into pMD19-T vector,transformed into E.coli DH5α competent cells.The recombinant plasmid was identified,and positive clones were selected for sequencing,DNAStar software was used to analyze the homology,amino acid sequence and phylogenetic tree of 13 ORFV genome sequences published on NCBI.The results showed that the nucleotide homology of B2L,F1L and VIR genes were 92.8% to 99.2%,95.7% to 99.5% and 77.6% to 100%,respectively.After comparing the amino acid sequence between the two genomes and the reference sequence,it was found that there were obvious differences in the immune related genes between the two genomes,and F1L gene had some rules to follow.The phylogenetic analysis of B2L,F1L and VIR genes showed that ORFV-Y was closely related to the Chinese Fujian goat strain,while ORFV-LT was far from the reference strains,and was a separate branch.The results showed that ORFV had obvious difference in immune related genes between sheep and camels,it provided a reference basis for further research on the changes of ORFV gene sequences in different species and the development of vaccines for different species in the future.     

四川省主要杂交稻组合DNA杂合性的比较

利用9个随机引物分析了近年来四川省选育的籼型杂交水稻新组合的DNA杂合性。结果表明,不同的引物揭示多态性的能力存在差异;同一引物在不同的水稻组合间扩增的多态性也不一样。14个组合DNA杂合性变异较小,分布于0.2283～0.3451之间。14个组合可以分为3类,但经t检验表明,13个组合与汕优63无显著差异。     

绿僵菌油剂防治新疆山地草原蝗虫的田间试验

用绿僵菌油剂在新疆山地草原对蝗虫进行了防治试验。喷药后采样,罩笼饲养从第3天开始感病死亡,第5天死亡明显增多,第6天绝大部分蝗虫死亡。该绿僵菌在自然条件下对试验区内的5种蝗虫具有相同的感染致病力。田间防效在第15天为60.9%,第18天为73.7%。     

云南派姬小蜂对其寄主及寄主植物相关化学线索的行为反应

与寄主植物和寄主相关的化学线索在寄生蜂的寄主搜寻过程中起着重要作用。云南派姬小蜂Pediobius yunnanensis是松树钻蛀性害虫——微红梢斑螟Dioryctria rubella的蛹寄生蜂,为了探究该寄生蜂对其寄主和寄主植物相关化学线索的行为反应,本研究进行了一系列试验。利用Y形嗅觉仪,测定了该寄生蜂对不同处理的油松球果（含蛹球果、含空蛹壳的球果、幼虫为害的球果和健康球果）和松枝（含蛹松枝、幼虫为害的松枝、含蛹球果的系统枝、含蛹松枝的系统枝和健康松枝）以及幼虫虫粪（3龄幼虫虫粪、含蛹球果内的幼虫虫粪）挥发物的趋性行为;在开放场中,测定了老熟幼虫爬行痕迹对该寄生蜂搜索行为的影响;利用双选择试验,在培养皿中测定了该寄生蜂对微红梢斑螟茧或蛹浸提液的行为反应。结果显示,在嗅觉试验中,含蛹球果、含有空蛹壳的球果挥发物对寄生蜂有极显著的引诱作用,而幼虫为害的球果和健康球果对寄生蜂的行为无明显影响;该寄生蜂对含蛹松枝挥发物表现出明显的正趋性反应,而对幼虫为害的松枝、含蛹球果的系统枝、含蛹松枝的系统枝以及健康松枝则无趋性反应;寄生蜂对含蛹球果中的幼虫虫粪挥发物表现出偏爱性,而对3龄幼虫虫粪无明显反应;在开放场中,老熟幼虫的爬行痕迹对寄生蜂的搜索行为无显著影响;在培养皿中,微红梢斑螟茧的甲醇、二氯甲烷或正己烷浸提液以及蛹的二氯甲烷或正己烷浸提液,不仅能强烈地吸引该寄生蜂,还能明显延长其停留时间。该结果为揭示云南派姬小蜂寄主搜寻行为的化学调控机制以及微红梢斑螟的生物防治提供理论依据。     

甘薯潜隐病毒莲藕分离物辅助成分-蛋白酶基因原核表达及抗血清制备

根据已报道的甘薯潜隐病毒莲藕分离物sweet potato latent virus-lotus(SPLV-lotus)核苷酸序列设计引物,采用RT-PCR从感病莲藕样品中扩增获得SPLV-lotus辅助成分-蛋白酶基因(HC-Pro),大小为1 375 bp。通过序列测定和分析后,将其克隆到原核表达载体pGEX4T-1,转化大肠杆菌Rosetta(DE3),经IPTG诱导并纯化获得大小为74 kD的融合蛋白pGEX4T-1-HC-ProSPLV-lotus。SDS-PAGE结果显示,融合蛋白获得过量表达。以纯化蛋白为抗原免疫新西兰大白兔制备抗血清,采用ELISA和Western blot对抗血清效价和特异性进行检测,当HC-Pro抗血清稀释比为1∶256 000时,ELISA显色后OD450读数仍高于0.6,表明所制备抗血清合格;Western blot结果显示,在感染SPLV-lotus植株样品中仍能检测到单一目的条带。这些结果表明,制备的抗血清效价合格且可用于SPLV-lotus的特异性检测。     

人孕酮受体激素结合区基因表达产物的检测与制备

PuPR为含有编码人孕激素受体（hPR）激素结合区（LBD）基因（631-933氨基酸）的pUC19重建质拉,在其宿主Escherichiacoli（BL21）中,经1mmol/L的IPTG诱导,该转化的细菌产生一C端为6×His结尾的蛋白质,分子量45KD。该表达产物可利用Ni-NTAHRP在ng水平用Western印迹检测,并可通过亲和层析的方法,利用His－bindingresin（Ni树脂）从菌体的裂解液中方便地回收到该蛋白质。     

干旱区农田蒸散量的日间变化分析

塔里木灌区位于中国新疆的塔克拉玛干沙漠北缘 ,本区开垦前是沙漠起伏、灌木丛生的原始荒漠 ,原始胡杨林点片连绵生长于荒漠之中。 1 95 2年 3月开垦至 1 996年 ,全灌区总灌溉面积已达到 660 0 0 ha。形成了稳定的农业生态环境 ,成为塔里木盆地最大的新绿洲。绿洲内部农田蒸散的日变化规律 ,极具干旱区特点。本文通过中国科学院阿克苏水平衡试验站的观测试验和一些干旱、半干旱及湿润地区的蒸发日变化过程进行了对比分析 ,论述了干旱区蒸发日变化的某些特征。