Extensification of grassland use in the Welsh uplands: sheep performance in years 1–6

An experiment was established in 1991 on a 25-year-old perennial ryegrass/bent ( Lolium perenne L. /Agrostis capillaris L.)-dominated pasture in Wales to study the effects of reducing nutrient inputs to previously fertilized upland pasture. The effects of the removal of applications of (1) N (denoted by CaPK) (2) N, P and K (Ca) and (3) N, P, K, and Ca (Nil) were compared with a treatment which received applications of all four nutrients (CaPKN) over a 6-year period (1991–96) in a randomized block design replicated three times. The experiment was managed under a continuous variable stocking regime (ewes and lambs until weaning and ewes thereafter) maintaining a sward surface height of 4·0 cm throughout the grazing season. Although individual liveweight gain of the lambs was unaffected by the treatments, there was a significant reduction ( P  < 0·05) in total lamb liveweight gain, ewe stocking rate and length of grazing season as a result of the withdrawal of nutrients. Over the 6 years total lamb liveweight gain was reduced by 17%, 32% and 45% and ewe stocking rate by 21%, 36% and 49% on treatments CaPK, Ca and Nil, respectively, compared with treatment CaPKN. The effect of withdrawing nutrient inputs on ewe stocking rate was progressive and by 1996 the Nil input treatment displayed a 63% reduction compared with the CaPKN treatment and this was also coupled with a 21-day reduction in length of the grazing season. During the post-weaning period, ewes from the Nil input treatment recorded a liveweight loss in 1995 and only a modest liveweight gain in 1996. This coupled with significantly lower body condition scores ( P  < 0·01) of these ewes in the autumn indicated that the Nil input treatment could lead to reductions in reproductive performance.     

Effects of Rice Variety and Growth Location in Cambodia on Grain Composition and Starch Structure

The effects of variety and growth location on grain composition and starch structures were investigated using three rice （Oryza sativa L.） cultivars （Phka Romduol, Sen Pidao and IR66） with different amylose contents. All the three cultivars were planted in three different agro-climatic zones （Phnom Penh, Coastal and Plateau） of Cambodia. The protein content of polished grains increased when rice was planted at a location with higher average temperature, but their lipid content decreased. The amylose content and degree of branching were not greatly affected by the minor temperature differences among the growing locations. Starch fine structures characterized by the chain-length distribution were significantly different among the cultivars, but not significantly among different locations. The results suggested that protein and lipid biosyntheses were more sensitive to the environmental temperature than that of starch in rice grains.     

Diagnostic Goodness-of-Fit Tests for Joint Recapture and Recovery Models

Diagnostic goodness-of-fit tests for capture–recapture models are routinely used prior to model fitting and analysis. However, when data include a mixture of live recaptures and dead recoveries, it is frequently standard practice for the information from recoveries not to be used, so that tests are applied to the recapture data alone. We present new diagnostic tests for joint recapture–recovery data, which make full use of all of the data, and evaluate their power through simulation. The importance of including all available data is clearly shown. We see in addition that current procedures may fail to identify the correct model. The work is generalised to the case of multi-site joint recapture–recovery data and is illustrated on a data set of great cormorants. This article has supplementary material online.     

Belowground nematode herbivores are resistant to elevated atmospheric CO2 concentrations in grassland ecosystems

Grasslands are considered to be one of the most sensitive ecosystems to rising atmospheric CO₂ concentrations, since, in addition to direct effects of elevated CO₂ on plant growth, indirect increases in water availability as an effect of elevated CO₂ may enhance primary production and alter plant community composition in these typically dry ecosystems. Moreover, grasslands support large populations of belowground herbivores that consume a major portion of plant biomass. The direct trophic link between herbivores and plants suggests that primary consumers may be particularly sensitive to rising CO₂ concentrations; however, the responses of belowground grassland herbivores have rarely been investigated. Here, we report the response of a range of herbivorous nematode populations to elevated CO₂ concentrations from three distinct grassland experiments. The three studies each involved 5–6 years of CO₂ fumigation, utilized natural or representative plant and soil communities, and were sampled at the end of the growing season. In the vast majority of cases, elevated CO₂ did not affect the abundance of nematode families; only two nematode families were significantly influenced by CO₂ enrichment (Anguinidae increased in one case and Hoplolaimidae decreased in another). Similarly, elevated CO₂ did not influence the total abundance, family richness, diversity or plant parasitic index of the herbivorous nematode community. These neutral responses to CO₂ enrichment occurred despite increased root production in all three experiments, suggesting a simultaneous antagonistic mechanism may have operated, possibly decreased root quality and/or changes in the soil environment. Whatever the mechanism, our findings suggest that herbivorous nematodes in grassland ecosystems are resistant to rising atmospheric CO₂ concentrations.     

Transient elevation of carbon dioxide modifies the microbial community composition in a semi-arid grassland

Using open-top chambers (OTC) on the shortgrass steppe in northern Colorado, changes of microbial community composition were followed over the latter 3 years of a 5-year study of elevated atmospheric CO₂ as well as during 12 months after CO₂ amendment ended. The experiment was composed of nine experimental plots: three chambered plots maintained at ambient CO₂ levels of 360±20 μmol mol^?1 (ambient treatment), three chambered plots maintained at 720±20 μmol mol^?1 CO₂ (elevated treatment) and three unchambered plots. The abundance of fungal phospholipid fatty acids (PLFAs) shifted in the shortgrass steppe under the influence of elevation of CO₂ over the period of 3 years. Whereas the content of the fungal signature molecule (18:2ω6) was similar in soils of the ambient and elevated treatments in the third year of the experiment, CO₂ treatment increased the content of 18:2ω6 by around 60% during the two subsequent years. The shift of microbial community composition towards a more fungal dominated community was likely due to slowly changing substrate quality; plant community forage quality declined under elevated CO₂ because of a decline of N in all tested species as well as shift in species composition towards greater abundance of the low forage quality species (Stipa comata). In the year after which CO₂ enrichment had ceased, abundances of fungal and bacterial PLFAs in the post-CO₂ treatment plots shifted slowly back towards the control plots. Therefore, quantity and quality of available substrates had not changed sufficiently to shift the microbial community permanently to a fungal dominated community. We conclude from PLFA composition of soil microorganisms during the CO₂ elevation experiment and during the subsequent year after cessation of CO₂ treatment that a shift towards a fungal dominated system under higher CO₂ concentrations may slow down C cycling in soils and therefore enhance C sequestration in the shortgrass steppe in future CO₂-enriched atmospheres.     

Cancer regression in patients after transfer of genetically engineered lymphocytes

Through the adoptive transfer of lymphocytes after host immunodepletion, it is possible to mediate objective cancer regression in human patients with metastatic melanoma. However, the generation of tumor-specific T cells in this mode of immunotherapy is often limiting. Here we report the ability to specifically confer tumor recognition by autologous lymphocytes from peripheral blood by using a retrovirus that encodes a T cell receptor. Adoptive transfer of these transduced cells in 15 patients resulted in durable engraftment at levels exceeding 10% of peripheral blood lymphocytes for at least 2 months after the infusion. We observed high sustained levels of circulating, engineered cells at 1 year after infusion in two patients who both demonstrated objective regression of metastatic melanoma lesions. This study suggests the therapeutic potential of genetically engineered cells for the biologic therapy of cancer.     

First detection of Edwardsiella ictaluri (Proteobacteria: Enterobacteriaceae) in wild Australian catfish

The bacterium Edwardsiella ictaluri is considered to be one of the most significant pathogens of farmed catfish in the United States of America and has also caused mortalities in farmed and wild fishes in many other parts of the world. E. ictaluri is not believed to be present in wild fish populations in Australia, although it has previously been detected in imported ornamental fishes held in quarantine facilities. In an attempt to confirm freedom from the bacterium in Australian native fishes, we undertook a risk‐based survey of wild catfishes from 15 sites across northern Australia. E. ictaluri was detected by selective culturing, followed by DNA testing, in Wet Tropics tandan (Tandanus tropicanus) from the Tully River, at a prevalence of 0.40 (95% CI 0.21–0.61). The bacterium was not found in fishes sampled from any of the other 14 sites. This is the first report of E. ictaluri in wild fishes in Australia.     

Ammonium and nitrate transport during saturated and unsaturated water flow through sandy soils

Citrus production in Florida accounts for ≈ 60% of national production in USA. The sandy soil characteristic (> 95% sand) makes water and nutrient management extremely difficult, raising concerns about environmental sustainability as a result of nutrient inputs in citrus producing regions where sandy soils dominate. Thus, laboratory column and field experiments were conducted to better understand the leaching patterns of and ions in Florida's sandy soils. The soil columns were first saturated from the bottom with two pore volumes of simulated Florida rain followed by pumping a pulse of fertilizer mixture at a steady Darcy flux of 14 cm h^?1. Nitrate and Cl^? appeared earlier in the effluent than in the A and B_h horizons, due to cation exchange of . Essentially identical breakthrough curves (BTCs) for and were observed in the E‐horizon, due to very low sorption of . The convective and dispersive equilibrium (CDE) model simulations were in good agreement with measured breakthrough curves (BTCs) for , , and Cl^?. However, the sorption coefficient (K_D) values used in the CDE model to simulate the BTCs for were about 10 times less than the batch isotherm K_D values. This was attributed to differences in pH, cation composition, and ionic strength between batch (static) and dynamic (leaching) systems. The field experiment showed that under unsaturated flow, improved short‐pulse fertigation systems (drip and microsprinkler) limited and transport beyond the root zone (top 30 cm), which might have promoted nutrient and water uptake in citrus. The column study revealed that under extreme weather events such as hurricanes or storm surge in Florida, saturated soil conditions can trigger N mobility below the root zone to surficial or groundwater aquifers. In the field experiment, the use of judicious, minimal and split applications and accurate placement of N‐fertilizers reduced leaching of N especially during heavy storms in the summer rainy months of Florida. The field experiment demonstrated that it is possible to manage inorganic N forms for optimal residence time for uptake and minimal leaching concerns.     

Rapid Evaporative Ionization Mass Spectrometry (REIMS): a Potential and Rapid Tool for the Identification of Insecticide Resistance in Mosquito Larvae

Insecticide resistance is a significant challenge facing the successful control of mosquito vectors globally. Bioassays are currently the only method for phenotyping resistance. They require large numbers of mosquitoes for testing, the availability of a susceptible comparator strain, and often insectary facilities. This study aimed to trial the novel use of rapid evaporative ionization mass spectrometry (REIMS) for the identification of insecticide resistance in mosquitoes. No sample preparation is required for REIMS and analysis can be rapidly conducted within hours. Temephos resistant Aedes aegypti (Linnaeus) larvae from Cúcuta, Colombia and temephos susceptible larvae from two origins (Bello, Colombia, and the lab reference strain New Orleans) were analyzed using REIMS. We tested the ability of REIMS to differentiate three relevant variants: population source, lab versus field origin, and response to insecticide. The classification of these data was undertaken using linear discriminant analysis (LDA) and random forest. Classification models built using REIMS data were able to differentiate between Ae. aegypti larvae from different populations with 82% (±0.01) accuracy, between mosquitoes of field and lab origin with 89% (±0.01) accuracy and between susceptible and resistant larvae with 85% (±0.01) accuracy. LDA classifiers had higher efficiency than random forest with this data set. The high accuracy observed here identifies REIMS as a potential new tool for rapid identification of resistance in mosquitoes. We argue that REIMS and similar modern phenotyping alternatives should complement existing insecticide resistance management tools.     

Dietary soluble non-starch polysaccharide level and xylanase supplementation influence performance,egg quality and nutrient utilization in laying hens fed wheat-based diets

The aim of this study was to evaluate the effects of dietary soluble non-starch polysaccharide (sNSP) content and xylanase supplementation on production performance, egg quality parameters, and nutrient digestibility in Hy-line Brown layers from 25 to 32 wk of age. A total of 144 Hy-line Brown laying hens (25 wk of age) were randomly allocated to 1 of 4 wheat-based dietary treatments in a 2 × 2 factorial experimental design, with 36 replicates of individual hens per treatment. The diets were formulated to contain either a high or low sNSP level (at 13.3 or 10.8 g/kg) and were supplemented with either 0 or 12,000 BXU/kg exogenous xylanase. Birds were fed these treatment diets for an 8-wk period, and hen production performance, including daily egg production, average egg weight, daily egg mass, feed conversion ratio and proportion of dirty and abnormal eggs were measured at bird age 25 to 28 wk and 29 to 32 wk. An interaction between sNSP content of the diet and xylanase supplementation was observed on daily egg production from 25 to 28 wk of age (P = 0.018); birds fed the high sNSP diet without xylanase had lower egg production than those fed any other treatment. An interaction between the 2 dietary factors was also observed on hen weight gain at 29 to 32 wk of age (P = 0.014), with birds fed the low sNSP diet with 12,000 BXU/kg xylanase presenting greater weight gain compared to those fed the high sNSP diet with 12,000 BXU/kg xylanase. Feed intake at 29 to 32 wk of age was reduced by xylanase supplementation (P = 0.047). Xylanase supplementation also increased yolk colour score at both 28 and 32 wk of age, and decreased yolk weight at 32 wk of age (P = 0.014, 0.037 and 0.013, respectively). Birds fed the low sNSP diet presented lower protein digestibility (P = 0.024) than those fed the high sNSP diet. Additionally, birds fed high sNSP presented higher shell reflectivity at both 28 and 32 wk of age (P = 0.05 and 0.036, respectively). The influence of duration of feeding the treatment diets on egg quality was also determined. It was observed that egg weight, yolk weight and yolk colour score consistently increased over time, regardless of experimental treatment effects. In contrast, Haugh Unit and albumen height significantly decreased throughout the study period in all treatments, although this was less pronounced in hens fed the treatment with high sNSP and no supplemental xylanase. A reduction in shell breaking strength over time was observed only in hens fed the treatments without xylanase addition, and shell thickness was improved over time only in birds fed the low sNSP diet with xylanase. The impacts of the dietary treatments were largely inconsistent in this study, so a solid conclusion cannot be drawn. However, these findings do indicate that dietary NSP level influences layer production performance, and thus should be considered when formulating laying hen diets. It also proved that further research is warranted into how to optimize the benefits of xylanase application in laying hens.