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71.
Normal mixtures are applied in interval mapping to model the segregation of genotypes following Mendel's Law in successive
generations of crossing. Standard methods use least squares or maximum likelihood estimates. Theoretically, maximum likelihood
is known to result in more efficient estimates than least squares. In the interval mapping literature, some authors state
that both methods yield equivalent results, whereas other authors emphasize the higher efficiency of maximum likelihood. The
present paper investigates differences of both methods more closely.
We show by example the occurrence of multiple LOD-Score profiles when applying maximum likelihood estimation methods for a
basic interval mapping and composite interval mapping model. This analysis results in some peaks of the LOD-Score profile
that distinctly differ from F-statistic profiles without being spurious. A spurious profile for IM and CIM was found as well. It is concluded that users
of IM and CIM mapping software must be prepared for the rare occurrence of spurious solutions in LOD profiles. The example
indicates that especially in sparse marker maps maximum likelihood estimation has a potential to result in non-spurious profiles
that are not similar to the F-statistic profiles. However, the discrimination of spurious and non-spurious further profiles is not straightforward in applications.
Until the mathematical background of this phenomenon is investigated more thoroughly and rules to ascertain the information
content of these additional solutions have been developed, the simultaneous use of the least squares and ML methods may caution
against the rare occurrence of spurious results.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献
72.
Vallentin Claudia Harfenmeister Katharina Itzerott Sibylle Kleinschmit Birgit Conrad Christopher Spengler Daniel 《Precision Agriculture》2022,23(1):52-82
Precision Agriculture - Information provided by satellite data is becoming increasingly important in the field of agriculture. Estimating biomass, nitrogen content or crop yield can improve farm... 相似文献
73.
Alarcon P Velasova M Mastin A Nevel A Stärk KD Wieland B 《Preventive veterinary medicine》2011,101(3-4):182-191
A cross-sectional study involving 147 pig farms across England was conducted in 2008-2009. Farm severity of post-weaning multi-systemic wasting syndrome (PMWS) was estimated through the use of an algorithm that combined data on post-weaning mortality, PMWS morbidity and proportion of porcine circovirus type 2 PCR positive pigs. Farms were classified as non/slightly, moderately or highly affected by PMWS. Data on potential PMWS risk factors were collected through interviews, on-farm assessment and serological sampling. Risk factors were identified using multivariable ordinal logistic regression and multivariable linear regression. Factors associated with increased PMWS severity were rearing growers indoors (OR=23.7), requiring a higher number of veterinarian visits per year (OR=9.6), having poorly isolated hospital pens (OR=6.4), buying replacement boars (OR=4.8) and seropositivity to Mycoplasma hyopneumoniae (OR=4.29); factors associated with decreased PMWS severity were low stocking density for growers (OR=0.07), adjusting diets at least three times between weaning and 14 weeks of age (OR=0.12), and requiring visitors to be at least 2 days pig free (OR=0.14). This study provides evidence of the association between environmental and management factors and PMWS severity, and suggests that other pathogens may be important co-factors for the disease. In addition, this study highlights the potential efficacy of biosecurity measures in the reduction/prevention of within-farm PMWS severity. 相似文献
74.
Lorenz K Bader M Klaus A Weiss W Görg A Hofmann T 《Journal of agricultural and food chemistry》2011,59(18):10219-10231
Saliva flow induced by 6-gingerol (pungent), hydroxy-α/β-sanshools (tingling), and citric acid (sour) was measured, and the time-dependent changes in the whole saliva proteome were analyzed by means of 2D-PAGE, followed by tryptic in-gel digestion and MALDI-TOF-MS peptide mass fingerprint analysis. The proteins showing significantly decreased abundance after oral 6-gingerol stimulation were identified as glutathione S-transferase P, the heat shock protein β-1, the heat shock 70 kDa protein 1, annexin A1, and cytoplasmic β-actin, whereas prolactin inducible proteins (PIP), short palate, lung and nasal epithelium carcinoma-associated protein 2 (SPLUNC2), zinc-α-2-glycoproteins (Zn-α-GP), and carbonic anhydrase VI (CAVI) were found with increased abundance. As the effects of this study were observed instantaneously upon stimulation, any proteome modulation is very likely to result from the release of proteins from preformed vesicles and not from de novo synthesis. The elevated levels of SPLUNC2, Zn-α-GP, and CAVI might be interpreted to trigger innate protective mechanisms in mucosal immunity and in nonimmune mucosal defense and might play an important role during the initial stage of inflammation. 相似文献
75.
Weisrock KU Winkelsett S Martin-Rosset W Forssmann WG Parvizi N Coenen M Vervuert I 《Veterinary journal (London, England : 1997)》2011,190(2):e130-e134
Intermittent administration of parathyroid hormone (PTH) is an anabolic therapy for osteoporotic conditions in humans. This study evaluated the effects of equine PTH fragment (ePTH-1-37) administration on bone metabolism in 12 healthy horses. Six horses each were treated once daily for 120days with subcutaneous injections of 0.5μg/kg ePTH-1-37 or placebo. Blood was collected to determine ionized calcium (Ca(++)), total Ca (Ca(T)), inorganic phosphorus, serum equine osteocalcin (eOC), carboxy-terminal telopeptide of type I collagen (ICTP), bone-specific alkaline phosphatase, and carboxy-terminal cross-linked telopeptide of type I collagen. Bone mineral density (BMD) was determined with dual X-ray absorptiometry of the metacarpus and calcaneus. Significantly higher blood Ca(++) and plasma Ca(T) concentrations were measured 5h after ePTH-1-37 administration compared to placebo. Higher serum eOC concentrations were found for ePTH-1-37 treatment at days 90 (P<0.05) and 120 (P=0.05). Significantly higher serum ICTP levels were observed with ePTH-1-37 treatment at days 60 and 90. For both study groups, BMD increased significantly in the calcaneus. Long-term use of ePTH-1-37 seemed to have no negative effects on bone metabolism in healthy horses. The absence of undesirable side effects is the premise to ensure safety for further clinical investigations in horses with increased bone resorption processes. 相似文献
76.
Shirasuna K Watanabe S Nagai K Sasahara K Shimizu T Ricken AM Spanel-Borowski K Miyamoto A 《The Journal of reproduction and development》2007,53(6):1319-1328
Cell-to-cell interaction via cell contact-dependent pathway is essentially important for maintenance and regulation of corpus luteum (CL) integrity and its physiological actions. The objective of the present study was to evaluate the mRNA expression of the cell adhesion molecules (CAMs) that are constituent factors of gap junctions [connexin (Cx) 43] and adherence junctions (VE-, E-, N-cadherin) in two types of endothelial cells from the mid CL and in CL tissue during the estrous cycle and PGF(2alpha)-induced luteolysis in the cow. Specific mRNA expression for Cx43 and N-cadherin was detected in cytokeratin-positive (CK+) and cytokeratin-negative (CK-) luteal endothelial cells (EC) and fully luteinized granulosa cells (LGC). E-cadherin mRNA was expressed in CK+EC and LGC, but not in CK-EC. VE-cadherin mRNA was expressed in both CK+ and CK-EC. During the estrous cycle, Cx43 mRNA expression was significantly lower in the regressing CL. VE-cadherin expression also tended to increase in the mid CL and increased significantly in the regressing CL. E-cadherin mRNA expression was higher in the early and late CL than in the mid- and regressing CL. N-cadherin mRNA expression gradually increased from the early to late CL followed by a decrease in the regressing CL. During PGF(2alpha)-induced luteolysis, Cx43 mRNA expression appeared to increase, and VE-cadherin and E-cadherin mRNA significantly increased at 24 h. N-cadherin mRNA expression decreased 2 and 4 h after PGF(2alpha) administration. Collectively, expression of the mRNAs for CAMs was different in the two types of luteal endothelial cells and fully luteinized granulosa cells and changed independently in the CL during the estrous cycle and PGF(2alpha)-induced luteolysis in the cow. The results suggest that CAMs play physiological roles in cell-to-cell communication to regulate both gap and adherence junctions during CL development and regression in the cow. 相似文献
77.
Velia-Isabel Hülsmeyer Katharina Flatz Katrin Putschbach Martina Ramona Bechter Sebastian Weiler Andrea Fischer Melanie Feist 《Irish veterinary journal》2015,68(1)
A 6-week-old female Simmental calf was evaluated for acute non-ambulatory tetraparesis. Physical and laboratory examinations revealed no clinically relevant abnormalities. Neurological findings were consistent with acute, progressive and painful cervical myelopathy. Radiographs displayed a fractured odontoid process (dens axis) and vertebral step misalignment at the fracture site. A traumatic origin was suspected. Advanced diagnostic imaging was considered to allow better planning of potential surgical stabilisation and to exclude any additional lesions of the cervical vertebral column. However, during trailer transportation to the advanced diagnostic imaging and surgery site, the calf deteriorated neurologically and was humanely euthanised. Magnetic resonance imaging (MRI) and computed tomography (CT) were performed immediately post-mortem for scientific reasons. The MRI examination reflected the radiographic findings and confirmed severe spinal cord compression at the fracture site. In addition, a T2W-hyperintense signal change within the paravertebral soft tissue dorsal to the fracture site was indicative of a traumatic event. CT identified the fracture site at the synchondrosis between the odontoid process and the body of the axis, and this finding was confirmed by post-mortem examination. Advanced diagnostic imaging and post-mortem examination did not identify any other cervical lesion. In summary, this calf was diagnosed with a traumatic odontoid process synchondrosis fracture, which has not been reported previously in calves but presents a challenging and well-known fracture type in young children. This case report indicates that the odontoid process synchondrosis is a potential predisposed injury site and that traumatic odontoid process synchondrosis fractures should be considered as a potential differential in calves with acute cervical pain and/or signs of a cervical myelopathy. 相似文献
78.
79.
Surface sealing and runoff generation on soils derived from loess and pleistocene deposits An attempt is undertaken to bring together existing knowledge on the occurence of surface sealing, on subprocesses and factors controlling surface sealing, and on hydraulic properties of seals, with special reference to the soil conditions prevailing in Germany. A conceptional model is proposed that relates different subprocesses such as aggregate breakdown, aggregate deformation, particle rearrangement and different factors such as soil cover, microrelief, stone cover, and macropores to surface sealing and runoff generation. We conclude that loess soils and soils formed on pleistocene deposits are particularly prone to surface sealing, and seals formed on these soils can induce runoff at rainfall intensities of 5 mm h?1. 相似文献
80.