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191.
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193.
Hossam EL-SHEIKH ALI Go KITAHARA Youji TAMURA Ikuo KOBAYASHI Koichiro HEMMI Shidow TORISU Hiroshi SAMESHIMA Yoichiro HORII Samy ZAABEL Shunichi KAMIMURA 《The Journal of reproduction and development》2013,59(1):59-65
The aim of the present study was to describe the temperature of the different portions
of the female genital tract and their relation to rectal temperature and to investigate
the effect of steroid hormones profiles on these variables over the estrous cycle in
cattle. Four nonpregnant Japanese Black cows were investigated daily over two successive
estrous cycles using a digital thermometer with a long probe and rounded-end sensor to
record the temperature of the rectum (RT), vagina (VT), cervix (CT), uterine body (UBT)
and uterine horns (UHT). Blood samples were collected immediately before temperature
recording to assay peripheral levels of progesterone (P4) and estradiol-17β
(E2). Moreover, transrectal ultrasonography was carried out after temperature
recording to monitor the ovulatory follicle and track ovulation. During the experiment,
the ambient temperature and relative humidity were recorded for further calculation of the
temperature humidity index (THI). The temperature within the genital tracts in these cows
progressively increased towards the uterine horns from the vagina. The VT, CT, UBT and
UHTs were significantly higher in association with peripheral P4 concentrations
greater than 4 ng/ml (mid-luteal phase) when compared with lower peripheral P4
concentrations. The VT was more significantly (P<0.01) correlated to the CT, UBT and
UHTs than RT. In conclusion, a temperature gradient was present among the vagina, cervix
and uterus over the estrous cycle, and changes in peripheral P4 concentrations
were associated with the thermal variations within these portions. The VT could be more
beneficial than RT in monitoring temperature of deeper portions of the female genital
tract in bovine. 相似文献
194.
195.
Atsuo IIDA Hitoshi TAKEMAE Ronald TARIGAN Ryosuke KOBAYASHI Hirokazu KATO Hiroshi SHIMODA Tsutomu OMATSU Supratikno Chaerul BASRI Ni Luh Putu Ika MAYASARI Srihadi AGUNGPRIYONO Ken MAEDA Tetsuya MIZUTANI Eiichi HONDO 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2021,83(7):1068
Here, we performed next-generation sequencing (NGS) on six large flying foxes (Pteropus vampyrus) collected in Indonesia. Seventy-five virus species in the liver tissue of each specimen were listed. Viral homologous sequences in the bat genome were identified from the listed viruses. This finding provides collateral evidence of viral endogenization into the host genome. We found that two of the six specimens bore partial sequences that were homologous to the plant pathogens Geminiviridae and Luteoviridae. These sequences were absent in the P. vampyrus chromosomal sequences. Hence, plant viral homologous sequences were localized to the hepatocytes as extrachromosomal DNA fragments. Therefore, this suggests that the bat is a potential carrier or vector of plant viruses. The present investigation on wild animals offered novel perspectives on viral invasion, variation, and host interaction. 相似文献
196.
YAN LU MD BRETT NEMKE BS DOUGLAS M. LORANG PhD ROEL TRIP MD HIROHITO KOBAYASHI MS MARK D. MARKEL DVM PhD Diplomate ACVS 《Veterinary surgery : VS》2009,38(4):467-476
Objectives— To compare bone healing of tibial osteotomy repaired with Nitinol wire braid and hardened steel rods (Braid system) and polymethylmethacrylate bone cement with an interlocking intramedullary (IM) nail fixation in an ovine model.
Study Design— In vitro and in vivo experimental study.
Animals— Adult female sheep (n=22).
Methods— Using sheep tibia, a middiaphyseal transverse osteotomy was performed in the right tibia, which were then randomly assigned to the Braid system group or IM nail group (n=5). The left tibia were used as controls. The torsional properties of tibial constructs were compared. The study was repeated in vivo in 12 sheep and mechanical properties and bone healing were evaluated at 12 weeks.
Results— In vitro, there was no significant difference in torsional stiffness between the groups. In vivo, operative time for the Braid system group was significantly shorter than the IM nail group. At 12 weeks, there were no significant differences in maximum torque and torsional stiffness between IM nail and Braid system groups nor were there significant radiographic or histologic differences between the groups.
Conclusions— The Braid system might decrease operative time for repair of transverse middiaphyseal tibial fractures and result in similar bone healing at 12 weeks after surgery compared with an interlocking IM nail repair.
Clinical Relevance— A Nitinol Braid system may be a treatment option for transverse midshaft tibial fractures. 相似文献
Study Design— In vitro and in vivo experimental study.
Animals— Adult female sheep (n=22).
Methods— Using sheep tibia, a middiaphyseal transverse osteotomy was performed in the right tibia, which were then randomly assigned to the Braid system group or IM nail group (n=5). The left tibia were used as controls. The torsional properties of tibial constructs were compared. The study was repeated in vivo in 12 sheep and mechanical properties and bone healing were evaluated at 12 weeks.
Results— In vitro, there was no significant difference in torsional stiffness between the groups. In vivo, operative time for the Braid system group was significantly shorter than the IM nail group. At 12 weeks, there were no significant differences in maximum torque and torsional stiffness between IM nail and Braid system groups nor were there significant radiographic or histologic differences between the groups.
Conclusions— The Braid system might decrease operative time for repair of transverse middiaphyseal tibial fractures and result in similar bone healing at 12 weeks after surgery compared with an interlocking IM nail repair.
Clinical Relevance— A Nitinol Braid system may be a treatment option for transverse midshaft tibial fractures. 相似文献
197.
Saishu YOSHIDA Hideaki YURINO Masaaki KOBAYASHI Naoto NISHIMURA Kentaro YANO Ken FUJIWARA Shin-ichi HASHIMOTO Takako KATO Yukio KATO 《The Journal of reproduction and development》2022,68(3):225
Pituitary endocrine cells are supplied by Sox2-expressing stem/progenitor cells in the anterior lobe of the adult pituitary gland. These SOX2-positive cells are maintained in two types of microenvironments (niches): the marginal cell layer (MCL)-niche and the parenchymal-niche. Recently, we isolated dense SOX2-positive cell clusters from the parenchymal-niche by taking advantage of their resistance to protease treatment as parenchymal stem/progenitor cell (PS)-clusters. In the present study, by analyzing these isolated PS-clusters, we attempted to identify novel structural characteristics of pituitary stem/progenitor cell niches. Quantitative real-time PCR showed that tight junction-related genes were distinctly expressed in the isolated PS-clusters. Immunocytostaining showed that the tight junction molecules, ZO-1 and occludin, were localized in the apical membrane facing the pseudo-follicle-like structure of the isolated PS-clusters regardless of the expression of S100β, which distinguishes the sub-population of SOX2-positive cells. Furthermore, immunohistochemistry of the pituitary glands of adult rats clearly demonstrated that ZO-1 and occludin were densely present in the parenchymal-niche encircling the pseudo-follicle, while they were observed in the apical membrane in the MCL-niche facing the residual lumen. Collectively, these tight junction-related proteins might be involved in the architecture and maintenance of the plasticity of pituitary stem/progenitor cell niches. 相似文献