禄丰县森林防火工作现状分析与发展思路探讨

森林防火工作是保护森林资源和发展林业事业的基础工作,重视和加强森林防火工作,对保护森林资源安全、巩固林业建设成果、改善生态环境,促进社会经济可持续发展,具有十分重要的意义。本文在对禄丰县森林防火工作现状进行分析的基础上,结合县情,从主、客观方面对存在问题进行了剖析,对今后森林防火工作中应采取的措施和对策进行了探讨。     

罗非鱼催乳素I基因的组织分布及盐胁迫对其表达的影响

采用同源克隆方法从橙色莫桑比克罗非鱼（Oreochromis mossambicus）、荷那龙罗非鱼（O.hornorum）及其正反交子代垂体中克隆了催乳素Ⅰ基因（PRLⅠ）,并对其核苷酸序列和推导的氨基酸序列进行了比对分析。结果显示,4种罗非鱼的PRLⅠ基因序列长度均为798 bp,ORF长639 bp,共编码212个氨基酸;橙色莫桑比克罗非鱼和荷那龙罗非鱼仅在31号位点存在一个氨基酸残基差异。用qPCR方法分析橙色莫桑比克罗非鱼和荷那龙罗非鱼PRLⅠ基因在不同组织或器官中的表达,结果表明罗非鱼PRLⅠ基因在垂体中的表达量最高,在其他组织中的表达量较低;盐胁迫后4种罗非鱼垂体中的PRLⅠmRNA水平均显著降低,而在其他组织中的表达变化不大。由此可知PRLⅠ基因主要在垂体表达,参与罗非鱼的渗透压调节。     

苗木质量、机械化抚育对营林生产的影响

阐述了机械化抚育、树种选择及苗木储存的方法,并提出了幼林抚育措施。     

Antiproliferation effects of 188Re labeled monoclonal antibody on prostate cancer cells in vitro

AIM: To investigate the effect of 188Re labeled monoclonal antibody on prostatic specific membrane antigen 7E11C5.3,radioimmunotherapy for the treatment of human prostate cancer cell line LNCaP in vitro.METHODS: 188Re-7E11C5.3 was prepared by direct 2-mercaptoethanol reduction method.Labeling efficiency and radiochemical purity was measured by paper chromatography.Immunoreactive fraction was determined by linear extrapolation.Cytotoxicity to LNCaP cells was determined by MTT assay.RESULTS: The labeling yield of 188Re-7E11C5.3 was (93.16±2.18)%,the radiochemical purity was (95.62±0.48)%,and the immunoreactive fraction was (74.86±1.86)%.The inhibitory effect of 188Re-7E11C5.3 on cell proliferation of LNCaP cells was significantly higher than that of 188Re-mIgG or 188ReO-4.The 50% inhibitory doses (IC50) of 188Re-7E11C5.3,188Re-mIgG,and 188ReO-4 were (23.38±3.73)×107 Bq/L,(59.21±8.02)×107 Bq/L and (68.89±10.91)×107 Bq/L,respectively.CONCLUSION: 188Re-7E11C5.3 can effectively inhibit the growth of in vitro cultured prostate cancer cells and shows much potential for prostate cancer radioimmunotherapy.     

秃杉种质资源迁地保存育苗技术研究

总结研究了13a来在地处亚热带在江苏省宁镇丘陵地区引种原产云贵高原、鄂西山地的珍稀树种秃杉获得在播种育苗与无性繁殖技术成果。该项 成果认为采用氧化剂消毒浸种侵芽,适时防治幼苗猝倒病,适度遮荫保湿,当年冬季防寒保苗等组装技术,可使每公斤种子产秃杉合格苗2．20万－5．60万株,1年生苗安全越冬;采用砻糠灰作基质,用KMnO4液浸条,在秋冬季扦插,生根率达41．3％－54．4％;用电子叶间隙喷雾,以珍珠岩作基质,或在日光温室内用塑料薄膜封闭保温,用KMnO4液浸条等技术,可使插条生根率达72．6％和76．1％。     

Effect of glycine on body weight and lipid metabolism in mice

AIM: To study the effect of glycine on body weight and lipid metabolism in mice. METHODS: Mice were randomly divided into four groups, control, high-fat diet group (HF), glycine group (Gly), and glycine treatment group (Gly+HF). The mice were fed with normal diet and high-fat diet for 12 days, respectively, and then were treated with glycine solution by intraperitoneal injection. Finally, the serum was collected to measure serum lipid, epididymal fat-pads, pararenal fat-pads and abdominal fat-pads were taken out to weight. Moreover, pathologic changes in liver were observed. RESULTS: The body weight of HF group was obviously lower than that in control; the body weight and adipose tissue content of Gly group were obviously lower than those in control; the body weight and adipose tissue content of Gly+HF group were obviously lower than those in HF group; the levels of TC and TG in Gly group were obviously lower than those in control; there was no statistical difference in serum lipid between Gly+HF and HF groups. In addition, marked hepatic steatosis in HF group, and slight hepatic steatosis in Gly+HF group were found. CONCLUSION: Gly can decrease the body weight and adipose tissue content and serum lipid in normal diet and high-fat diet mice, and it also can prevent hepatic steatosis.     

乳化-溶剂挥发法制备禽霍乱乙基纤维素微胶囊疫苗

为制备禽霍乱乙基纤维素微胶囊疫苗,笔者采用乳化 溶剂挥发法制备禽霍乱乙基纤维素微胶囊,对制得的微胶囊外形、粒径分布、包封率及缓释性能等进行了研究。该方法制成的微胶囊呈球形,外观圆整,流动性好,粒径分布范围为10～80 μm,包封率70％以上,体外释放72 h的释药率为39.72%。该方法重复性好,简单易行,体外释药试验结果表明,所得的微胶囊疫苗有明显的缓释作用。