Glucagon stimulation test for estimating endogenous insulin secretion in dogs

Fifty-one dogs (27 diabetic dogs, four that had recovered from diabetes and 20 healthy control dogs) were given 0.5 or 1.0 mg glucagon intravenously. Blood samples were taken before the injection and 10 and 20 minutes after it. Samples were analysed to determine C-peptide, insulin and glucose concentrations, and one sample from each dog was analysed for fructosamine. The median (interquartile range) concentrations of C-peptide in the samples taken at 10 minutes were 0.5 (0.3 to 0.8) nmol/l in the control dogs, 0.1 (0 to 0.2) nmol/l in the diabetic dogs, and 0.3 (0.2 to 0.4) nmol/l in the dogs that had recovered from diabetes. Seven of the 51 dogs showed mild adverse reactions after the injection of glucagon.     

Use of topical selamectin for the treatment of Trichosomoides crassicauda infection in laboratory rats

The present study investigated the efficacy of topical selamectin for elimination of naturally acquired Trichosomoides crassicauda infection in rats. Twelve T. crassicauda-positive rats were assigned to the treatment group and six rats were assigned to the control group. Selamectin (6 mg/kg) was applied topically to the skin in a single spot at the base of the neck in front of the scapulae in the treatment group. To assess the efficacy of the treatment, animal faeces were investigated with the use of the flotation technique on days 0, 4, 14 and 24 after selamectin application. The rats of the treatment and control groups were necropsied on the day 24. In the treatment group, 7 of 12 infected rats were cured completely. Topical selamectin was found to be effective in eliminating T. crassicauda infection in rats.     

Promoter region of the bovine growth hormone receptor gene: single nucleotide polymorphism discovery in cattle and association with performance in Brangus bulls

Expression of the GH receptor (GHR) gene and its binding with GH is essential for growth and fat metabolism. A GT microsatellite exists in the promoter of bovine GHR segregating short (11 bp) and long (16 to 20 bp) allele sequences. To detect SNP and complete an association study of genotype to phenotype, we resequenced a 1,195-bp fragment of DNA including the GT microsatellite and exon 1A. Resequencing was completed in 48 familialy unrelated Holstein, Jersey, Brown Swiss, Simmental, Angus, Brahman, and Brangus cattle. Nine SNP were identified. Phylogeny analyses revealed minor distance (i.e., <5%) in DNA sequence among the 5 Bos taurus breeds; however, sequence from Brahman cattle averaged 27.4 +/- 0.07% divergence from the Bos taurus breeds, whereas divergence of Brangus was intermediate. An association study of genotype to phenotype was completed with data from growing Brangus bulls (n = 553 from 96 sires) and data from 4 of the SNP flanking the GT microsatellite. These SNP were found to be in Hardy-Weinberg equilibrium and in phase based on linkage disequilibrium analyses (r(2) = 0.84 and D'= 0.92). An A/G tag SNP was identified (ss86273136) and was located in exon 1A, which began 88 bp downstream from the GT microsatellite. Minor allele frequency of the tag SNP was greater than 10%, and Mendelian segregation was verified in 3 generation pedigrees. The A allele was derived from Brahman, and the G allele was derived from Angus. This tag SNP genotype was a significant effect in analyses of rib fat data collected with ultrasound when bulls were ~365 d of age. Specifically, bulls of the GG genotype had 6.1% more (P = 0.0204) rib fat than bulls of the AA and AG genotypes, respectively. Tag SNP (ss86273136), located in the promoter of GHR, appears to be associated with a measure of corporal fat in Bos taurus x Bos indicus composite cattle.     

Effects of ammonia load on glucose metabolism by isolated ovine duodenal mucosa

To determine the effects of ammonia load on glucose metabolism in ruminant small intestinal tissues, duodenal mucosal cells (DMC) were isolated from growing female sheep (n = 10; 46. 0 +/- 0. 8 kg of BW) fed diets differing in CP content: high (19. 4%) vs. low (13. 1%). Ammonia concentration in the duodenal digesta fluid was greater for sheep fed a high CP diet compared with those fed a low CP diet (16. 4 +/- 1. 0 vs. 9. 1 +/- 1. 8 mM). The isolated primary mucosal cells were incubated for 90 min with [2-(13)C] glucose (3 mM) and ammonium chloride (0, 0. 1, 1, 5, 10, 20, or 50 mM) in Krebs-Ringer HEPES buffer. It was hypothesized that DMC would increase glucose carbon utilization for the synthesis of nonessential AA when the ammonia concentration in the incubation media increased. However, utilization of glucose carbon for alanine synthesis decreased linearly (P = 0. 03) as the ammonia concentration in the incubation media increased. Furthermore, glucose disappearance and utilization of glucose carbon for aspartate synthesis were not affected (P > 0. 47) by the ammonia concentration. Contrarily, in vitro glucose disappearance was greater (P = 0. 03) for DMC isolated from sheep fed a low CP diet vs. a high CP diet [14. 6 +/- 1. 6 vs. 8. 6 +/- 1. 3 nmol.(10(6) cells)(-1).(90 min) (-1)], and hexokinase activity was greater (P = 0. 01) in the mucosa of sheep fed a low CP diet compared with a high CP diet (1. 22 +/- 0. 05 vs. 1. 04 +/- 0. 02 mUnit/mg of protein). These observations indicate that ammonia load does not affect the extent of glucose utilization by DMC, and that glucose carbon may not play a significant role for the synthesis of alanine, aspartate, or glutamate when DMC are exposed to increased concentrations of ammonia.     

Interpopulation and intrapopulation maternal lineage genetics of the Lanyu pig (Sus scrofa) by analysis of mitochondrial cytochrome b and control region sequences

The Lanyu pig is an indigenous breed from the Lanyu Islet, which is southeast of Taiwan. Two herds of Lanyu pigs were introduced from the Lanyu Islet into Taiwan in 1975 and 1980. The current population of conserved Lanyu pigs consists of only 44 animals with unknown genetic lineage. The Lanyu pig possesses a distinct maternal genetic lineage remote from Asian and European pigs. The present study aimed to understand the phylogenetic relationship among conserved Lanyu, Asian, and European type pigs based on the cytochrome b coding gene, to ascertain the maternal lineage and genetic diversity within the conserved Lanyu pigs, and to address whether genetic introgression from exotic or Formosan wild pigs had occurred in the conserved Lanyu pigs. Entire mitochondrial genomes of both types of Lanyu pig comprised 2 ribosomal RNA, 22 transfer RNA, and 13 protein-coding genes. Only 2 haplotypes of the mitochondrial DNA (mtDNA) control region and cytochrome b were identified in the conserved Lanyu pig herds. When maximum likelihood trees were constructed, the Type I Lanyu mitochondrial genes formed a unique clade with a large pairwise distance of both cytochrome b and the control region from Asian and European type breeds, Formosan wild pigs, and exotic breeds. Significant loss of genetic diversity of mtDNA within the conserved Lanyu pigs was demonstrated by low haplotype and nucleotide diversities, supported by Fu and Li's D* neutrality test (1.44055; P < 0.05). The mtDNA control region sequences of extant pigs in the Lanyu Islet, however, showed high haplotype and nucleotide diversity, and clustered with exotic pigs. These results indicate no maternal lineage mtD-NA gene introgression from Formosan wild pigs and introduced exotic pigs to conserved Type I Lanyu pigs, and a severe loss of heterozygosity of mtDNA in conserved Lanyu pigs. The remaining extant pigs on the Lanyu Islet have been introgressed with exotic breeds. Strategies for future conservation of native Lanyu pigs are now even more urgent and important.     

Reducing phosphorus inputs for grazing Holstein steers

A 2-yr study was conducted to confirm that managed pastures can provide Holstein steers adequate P to meet their daily requirement. Treatments offered were trace mineralized salt with or without additional P. In the first year, 80 Holstein steers (248 kg of BW) were assigned to 4 grazing groups. Treatments were trace mineralized salt only or a 67:33 mixture of trace mineralized salt and dicalcium phosphate. Steers rotationally grazed a cool-season grass/legume mixture for 137 d. Fecal bags were placed on 3 steers from each grazing group (n = 12) over a 4-d period for estimation of forage DMI and forage contribution to daily P intake twice during the grazing season. Analyzed pasture samples contained 3.28 mg of P/g of DM. During the second year, 72 Holstein steers (297 kg of BW) were blocked into 2 BW groups and subsequently assigned to 1 of 4 pasture groups. Steers rotationally grazed the same forage base as the first year for 126 d. Pasture samples contained 3.27 mg of P/g of DM. No significant differences (P > 0.10) were detected for BW, ADG, or free-choice supplemental mineral intake. Forage provided 126% of the recommended NRC P requirement. Thus, supplemental phosphorous was not required for Holstein steers grazing mixed, cool-season, grass/legume pastures.     

Mineral content of hay harvested in Bavarian and Swiss horse farms. Predictive value of cutting time,number of cut,botanical composition,origin and fertilization

Hay samples from 29 horse farms in Southern Upper Bavaria and 31 horse farms in Switzerland were taken and analysed for minerals such as calcium, magnesium, phosphorus and potassium. The content of herbs and legumes in the hay was determined and the Weende analysis was performed. Comparison between Bavaria and Switzerland resulted in comparable calcium contents of approximately 4 g Ca/kg dry matter (DM) in grass hay. Hay with more than 10% of herbs and legumes found only in Switzerland showed higher Ca contents of >7 g Ca/kg DM in hay. The mineral contents of phosphorus and potassium were lower in hay from Switzerland (P: 1.8 ± 0.7 g/kg DM, K: 15.4 ± 5.1 g/kg DM) than from Bavaria (P: 3.8 ± 0.6 g/kg DM, K: 20.0 ± 6.0 DM), whereas the magnesium content of the hay showed no difference between the regions (∼1.5 g Mg/kg DM). Very late first grass hay cuts showed low magnesium and calcium (<4 g/kg DM) contents. Further minerals in the hay for horses differed from those in dairy cattle in the same region. Fertilization showed only small effects on the mineral contents of the hay produced for horses. Therefore, in calculation of ration and production of supplements for horses, these differences should be taken into account.     

Genetic analyses of piglet survival and individual birth weight on first generation data of a selection experiment for piglet survival under outdoor conditions

Genetic parameters of piglet survival traits and birth weight were estimated on the first generation data of a selection experiment aimed at improving piglet survival using a multiple trait linear and threshold model. Data on 5293 piglets for survival at birth, at day one after birth and during the entire nursing period, as well as individual birth weight and litter size, were recorded in an outdoor production system. Genetic effects of piglet survival traits and birth weight were estimated based on threshold and Gaussian models, respectively, using a Bayesian approach. The statistical model included as fixed effects selection group, parity, gender, fostering, gestation length and month of farrowing and, alternatively, an adjustment for litter size. Direct genetic effects (i.e. the piglet's genetic potential) for piglet survival and birth weight were estimated separately, whereas maternal genetic and environmental effects could only be estimated for the given data structure in a combined litter effect. Posterior means of heritabilities for direct genetic effects of survival at birth, at first day after birth and the entire nursing period, as well as birth weight, were 0.08, 0.07, 0.08 and 0.20, respectively. Genetic correlations among survival traits were in the range of 0.29 to 0.40 and indicate that these traits were mainly attributable to different genetic effects. Genetic correlations between direct effects of survival traits and birth weight ranged between 0.18 and 0.23 and were reduced when weights of stillborn piglets were omitted in the analysis or the traits were adjusted for litter size. The magnitudes of direct genetic effects of survival traits are substantially higher than estimates in the literature, which may indicate that these traits have a higher genetic influence under outdoor conditions. The use of birth weight in the multiple trait estimation provided important information for the estimation of survival traits due to its favourable genetic correlations with survival, its high heritability and its high information content as a continuously measured trait.     

Lack of detection of circulating skin-specific IgE autoantibodies in dogs with moderate or severe atopic dermatitis

Human patients with atopic dermatitis (AD) commonly exhibit IgE reactivity to cutaneous self-antigens. The presence of serum IgE autoantibodies appears to correlate with disease severity, and it is suspected to reflect or contribute to tissue damage. The objective of this study was to determine whether IgE autoantibodies specific for cutaneous antigens could be detected in the serum of dogs with AD. Serum was collected from 19 dogs with untreated moderate to severe AD and four specific-pathogen free (SPF) dogs. Indirect immunofluorescence was performed using normal canine skin collected at four different locations (concave ear, nose, medial thigh and lateral thorax), while Western immunoblotting was done using normal canine ear pinna epidermal and dermal extracts and reducing conditions. In both methods, IgE was detected using a monoclonal antibody specific for heat stable epitopes of canine IgE. At 1:10 dilution, specific IgE autoantibodies against cutaneous autoantigens were not detected, with either method, in AD and SPF canine sera. Either IgE autoreactivity is not associated with moderate to severe AD in dogs, or the methods employed herein were not sensitive enough to permit IgE autoantibody detection.