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11.
The specificity of the infection of maize, wheat and rice roots by N2-fixing Azospirillum spp was studied in four greenhouse experiments using pots with unsterilized soil and in two field experiments. In all experiments A. lipoferum was most frequently isolated from externally sterilized roots of maize, and A. brasilense nir? (nitrite reductase negative) from wheat and rice. In pot experiments, A. brasilense nir+ was isolated with moderate frequency from within maize roots but rarely from within wheat or rice roots. Inoculation of the pots with a mixture of representative strains of the three Azospirillum groups had no effect on the proportion of strains recovered from each plant species. In the field experiments, inoculation with spontaneous streptomycin-resistant mutants of two of the representative strains confirmed the apparent specificity of A. lipoferum for maize roots and of A. brasilense for wheat but the results were partially obscured by the unexpectedly high proportion of streptomycin-resistant strains isolated from within the roots of uninoculated plants. 相似文献
12.
Irene Vänninen Heikki Hokkanen Johanna Tyni‐Juslin 《Acta Agriculturae Scandinavica, Section B - Plant Soil Science》2013,63(3):167-183
Finnish isolates of Beauveria bassiana (8 isolates), Metarhizium anisopliae (7), Tolypocladium sp. (2), Paecilomyces farinosus (2), P. fumosoroseus (1), Steinernema feltiae (3) and Bacillus thuringiensis ('Muscabac') were tested for efficacy against mixed populations of Delia radicum and D. floralis under field conditions in 1986–90. All pathogens were applied preventatively, the fungi as aqueous conidial or mycelial suspensions, dry conidia or dry mycelial powder. In only two of the nine experiments did B. bassiana or M. anisopliae give some control. In 1986, B. bassiana SF85–2 and Tolypocladium sp. SF85–4 (both at rate 1.2 x 109 conidia plant‐ 1), and ‘Muscabac’ (25 g 1?1, 1 dl plant?1) reduced the number of pupae by 80%, 60% and 50%, respectively, as compared with untreated and chemical (isophenphos) controls. In 1990, M. anisopliae SF86–39 at rate 1.6 x 109conidia plant?1 and 1.5 x 108 CFU plant?1 and S. feltiae SFS‐22 (35000 plant?1), increased the yield of cauliflower 2.2, 1.8, and 2.3‐fold, respectively, as compared with the untreated control, but these yields were only 19%, 15% and 19% of those of the chemical (diazinon) control. Paecilomyces isolates were ineffective in the 1986 experiment in which they were included. Our results suggest that it is difficult to find efficient control agents among the fungal and nematode species tested for use as biopesticides against cabbage root flies, but that the potential of M. anisopliae against these pests deserves further study. 相似文献
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15.
Cooper JC 《The Canadian veterinary journal. La revue veterinaire canadienne》2002,43(9):712-714
A midline exploratory laparotomy on a 1-year-old, neutered male, West Highland terrier with a history of lethargy, anorexia, and intermittent vomiting revealed a cranial abdominal cyst. The lining of the excised cyst was histologically identical with that of the small intestine and may have represented an uncommon intestinal malformation. 相似文献
16.
Genetic resistance to diseases is a multigenic trait governed mainly by the immune system and its interactions with many physiologic and environmental factors. In the adaptive immunity, T cell and B cell responses, the specific recognition of antigens and interactions between antigen presenting cells, T cells and B cells are crucial. It occurs through a network of mediator proteins such as the molecules of the major histocompatibility complex (MHC), T cell receptors, immunoglobulins and secreted proteins such as the cytokines and antibodies. The diversity of these proteins that mainly is due to an intrinsic polymorphism of the genes causes phenotypic variation in disease resistance. The well-known linkage of MHC polymorphism and Marek's disease resistance difference represents a classic model revealing immunological factors in resistance differences and diversity of mediator molecules. The molecular bases in any resistance variation to infectious pathogens are vaguely understood. This paper presents a review of the major immune mediators involved in resistance and susceptibility to infectious diseases and their functional mechanisms in the chicken. The genetic interaction of disease resistance with production traits and the environment is mentioned. 相似文献
17.
Riikonen J Lindsberg MM Holopainen T Oksanen E Lappi J Peltonen P Vapaavuori E 《Tree physiology》2004,24(11):1227-1237
We studied the effects of elevated concentrations of carbon dioxide ([CO2]) and ozone ([O3]) on growth, biomass allocation and leaf area of field-grown O3-tolerant (Clone 4) and O3-sensitive clones (Clone 80) of European silver birch (Betula pendula Roth) trees during 1999-2001. Seven-year-old trees of Clones 4 and 80 growing outside in open-top chambers were exposed for 3 years to the following treatments: outside control (OC); chamber control (CC); 2 x ambient [CO2] (EC); 2 x ambient [O3] (EO); and 2 x ambient [CO2] + 2 x ambient [O3] (EC+EO). When the results for the two clones were analyzed together, elevated [CO2] increased tree growth and biomass, but had no effect on biomass allocation. Total leaf area increased and leaf abscission was delayed in response to elevated [CO2]. Elevated [O3] decreased dry mass of roots and branches and mean leaf size and induced earlier leaf abscission in the autumn; otherwise, the effects of elevated [O3] were small across the clones. However, there were significant interactions between elevated [CO2] and elevated [O3]. When results for the clones were analyzed separately, stem diameter, volume growth and total biomass of Clone 80 were increased by elevated [CO2] and the stimulatory effects of elevated [CO2] on stem volume growth and total leaf area increased during the 3-year study. Clone 80 was unaffected by elevated [O3]. In Clone 4, elevated [O3] decreased root and branch biomass by 38 and 29%, respectively, whereas this clone showed few responses to elevated [CO2]. Elevated [CO2] significantly increased total leaf area in Clone 80 only, which may partly explain the smaller growth responses to elevated [CO2] of Clone 4 compared with Clone 80. Although we observed responses to elevated [O3], the responses to the EC+EO and EC treatments were similar, indicating that the trees only responded to elevated [O3] under ambient [CO2] conditions, perhaps reflecting a greater quantity of carbohydrates available for detoxification and repair in elevated [CO2]. 相似文献
18.
Depending on the spatial density of built cover and location within a catchment, residential areas make varying contributions to surface runoff throughout different rainfall events. This study examined these contributions and the associated effect of different surface covers and sustainable drainage systems on runoff generation. The Soil Conservation Service curve number (SCS-CN) method was applied to analyse urban development in the Höjeå river catchment in southern Sweden.The results indicated that identical amounts of surface runoff are generated by low-density residential areas on heavy clay soils and high-density residential areas on sandy soils. However, increasing the density of built-up areas on sandy soils is likely to be more disruptive to the hydrological balance and to generate a greatly increasing difference in runoff as building density and impermeable surface area increase. A similar pattern is likely to occur if rainfall intensity increases. It may therefore be appropriate to apply different planning considerations to residential developments depending on the existing soil group, e.g. conserving existing vegetated surfaces on sandy soil and incorporating permeable paving materials and sub-surface infiltration beds in development on clayey soils. Increased area of impermeable surface cover will increase surface runoff in all residential areas, irrespective of building density, soil group and rainfall intensity. However, adapting a systems approach in combining vegetative structures and permeable paving materials with subsurface infiltration beds can help mitigate the impact of surface runoff, particularly in urban developments on clay-rich soils. 相似文献
19.
Tanja Grkovic Johanna S. Blees Magdalena M. Bayer Nancy H. Colburn Cheryl L. Thomas Curtis J. Henrich Megan L. Peach James B. McMahon Tobias Schmid Kirk R. Gustafson 《Marine drugs》2014,12(8):4593-4601
A cell-based high-throughput screen that assessed the cellular stability of a tumor suppressor protein PDCD4 (Programmed cell death 4) was used to identify a new guanidine-containing marine alkaloid mirabilin K (3), as well as the known compounds mirabilin G (1) and netamine M (2). The structures of these tricyclic guanidine alkaloids were established from extensive spectroscopic analyses. Compounds 1 and 2 inhibited cellular degradation of PDCD4 with EC50 values of 1.8 μg/mL and 2.8 μg/mL, respectively. Mirabilin G (1) and netamine M (2) are the first marine natural products reported to stabilize PDCD4 under tumor promoting conditions. 相似文献
20.
The aim of this study was to examine the embryogenic potential of floral material of the cassava cultivar MCOL 1505. Macerated
immature inflorescences were found to be highly embryogenic, with almost 78% of the explants producing somatic embryos. Somatic
embryos were also produced from whole male florets and half florets although at much lower rates. No regeneration was obtained
from anther, microspore or floret wall tissue. Somatic embryos derived from immature inflorescences were regenerated via organogenesis
and the plants derived from this process were assessed in terms of phenotype and ploidy level. If haploid plants could be
produced by this method, this would have significant implications in assisting traditional cassava breeding, as this would
allow homozygosity to be reached more rapidly. In a crop such as cassava, which is highly heterozygous in nature, the use
of haploids in a breeding programme could considerably shorten the time taken to produce new desirable cultivars. This is
the first report on plant regeneration through somatic embryogenesis from floral tissue of cassava.
This revised version was published online in July 2006 with corrections to the Cover Date. 相似文献