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31.
Berman DM Karhadkar SS Hallahan AR Pritchard JI Eberhart CG Watkins DN Chen JK Cooper MK Taipale J Olson JM Beachy PA 《Science (New York, N.Y.)》2002,297(5586):1559-1561
Constitutive Hedgehog (Hh) pathway activity is associated with initiation of neoplasia, but its role in the continued growth of established tumors is unclear. Here, we investigate the therapeutic efficacy of the Hh pathway antagonist cyclopamine in preclinical models of medulloblastoma, the most common malignant brain tumor in children. Cyclopamine treatment of murine medulloblastoma cells blocked proliferation in vitro and induced changes in gene expression consistent with initiation of neuronal differentiation and loss of neuronal stem cell-like character. This compound also caused regression of murine tumor allografts in vivo and induced rapid death of cells from freshly resected human medulloblastomas, but not from other brain tumors, thus establishing a specific role for Hh pathway activity in medulloblastoma growth. 相似文献
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33.
Fang W Vega-Rodríguez J Ghosh AK Jacobs-Lorena M Kang A St Leger RJ 《Science (New York, N.Y.)》2011,331(6020):1074-1077
Metarhizium anisopliae infects mosquitoes through the cuticle and proliferates in the hemolymph. To allow M. anisopliae to combat malaria in mosquitoes with advanced malaria infections, we produced recombinant strains expressing molecules that target sporozoites as they travel through the hemolymph to the salivary glands. Eleven days after a Plasmodium-infected blood meal, mosquitoes were treated with M. anisopliae expressing salivary gland and midgut peptide 1 (SM1), which blocks attachment of sporozoites to salivary glands; a single-chain antibody that agglutinates sporozoites; or scorpine, which is an antimicrobial toxin. These reduced sporozoite counts by 71%, 85%, and 90%, respectively. M. anisopliae expressing scorpine and an [SM1](8):scorpine fusion protein reduced sporozoite counts by 98%, suggesting that Metarhizium-mediated inhibition of Plasmodium development could be a powerful weapon for combating malaria. 相似文献
34.
A modification based on capillary flow type was proposed for three theoretical infiltration equations accounting for the physical properties of the soil. The equations were (a) Green and Ampt, (b) Fletcher, and (c) Fok and Hansen. The derived modified equations were tested by computer programs on four undisturbed field soils on which the mass infiltration and soil properties had been measured. Of the three equations, (b) exhibited the best adherence to the data points and (a) and (c) were reasonably good. In general, the types of relations revealed by the field measurements were such as would tend to linearize if the log of the mass infiltration was plotted against the log of time. The breaks exhibited by some of the soils could be explained by changes in the soil properties at that point. 相似文献
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36.
Thomas B. Moorman Jennifer K. Cowan Ellen L. Arthur Joel R. Coats 《Biology and Fertility of Soils》2001,33(6):541-545
Pesticide contamination of soil and groundwater at agricultural chemical distribution sites is a widespread problem in the USA. Alternatives to land-farming or solid waste disposal include biostimulation and phytoremediation. This research investigated the ability of compost, corn stalks, corn fermentation byproduct, peat, manure, and sawdust at rates of 0.5% and 5% (w/w) to stimulate biodegradation of atrazine [6-chloro-N-ethyl-N'-(1-methyethyl)-1,3,5-triazine-2,4-diamine], metolachlor [2-chloro-N-(2-ethyl-6-methylphenyl)-N-(2-methoxy-1-methylethyl)acetamide], and trifluralin [2,6-dinitro-N,N-dipropyl-4-(trifluoromethyl)benzenamine] added as a mixture to soil. Initial concentrations were 175ᆾ mg atrazine kg-1 soil, 182ᆭ mg metolachlor kg-1 soil, and 165ᆫ mg trifluralin kg-1 soil. After amendment addition, 30% of the atrazine, 33% of the metolachlor, and 44% of the trifluralin was degraded over 245 days, which included 63 days' aging prior to amendment additions. Atrazine degradation was enhanced by 0.5% manure, 5% peat, and 5% cornstalk amendments compared to nonamended soils. Metolachlor degradation was enhanced by all amendments at the 5% level, except for compost and peat. Amendments had no effect on trifluralin degradation. The 5% addition of compost, manure, and cornstalks resulted in significant increases in bacterial populations and dehydrogenase activity. A second experiment compared the persistence of atrazine, metolachlor, and trifluralin applied in a mixture to their persistence in soil individually. A combined average of 123 mg atrazine kg-1 remained in soil treated with the three-herbicide mixture compared to 31 mg atrazine kg-1 remaining in soil treated with atrazine only. Atrazine mineralization and atrazine-degrading microorganisms were suppressed by high concentrations of metolachlor, but not by trifluralin. 相似文献
37.
Kelsey L Batson Hilda I Caldern Mike D Tokach Jason C Woodworth Robert D Goodband Steve S Dritz Joel M DeRouchey 《Journal of animal science》2021,99(5)
Two experiments were conducted to determine the effects of crude protein (CP) level in diets containing coarse wheat bran (CWB) with or without pharmacological levels of Zn (provided by zinc oxide: ZnO) on growth performance and fecal DM of nursery pigs. In experiment 1, 360 barrows (Line 200 × 400, DNA, Columbus, NE, initially 5.6 kg) were allotted to 1 of 6 dietary treatments from d 0 to 21 after weaning with 5 pigs per pen and 12 pens per treatment. Treatments included a positive control diet (21% CP) with 3,000 mg/kg Zn in phase 1 and 2,000 mg/kg in phase 2; negative control (21% CP) with 110 mg/kg added Zn, and 4 diets containing 4% CWB and 110 mg/kg added Zn formulated to contain 21%, 19.5%, 18%, or 16.5% CP. The 2 control diets and 21% CP CWB diet contained 1.40% standardized ileal digestible (SID) Lys in phase 1 and 1.35% SID Lys in phase 2, while the 19.5%, 18%, and 16.5% CP diets contained 1.33, 1.25 and 1.20% Lys, respectively, in both phases. Pigs fed the positive control diet containing pharmacological ZnO had increased (P < 0.05) ADG and G:F compared with the negative control and the 21% CP CWB diet. Reducing CP (concurrently with SID Lys) in diets containing CWB decreased ADG and G:F (linear, P = 0.002); however, fecal DM increased (linear, P = 0.005). In experiment 2, two groups of 300 and 350 pigs, initially 7.0 and 6.2 kg, respectively, were used with 5 pigs per pen and 26 pens per treatment. The objective was to determine if adding back essential AA would improve growth performance of pigs fed the low CP diets. All dietary treatments were fed for 13 days, contained 4% CWB, and consisted of: (1) positive control with 2,000 mg/kg of Zn and 21% CP (1.35% SID Lys); (2) no ZnO and 21% CP; and 3 diets with no ZnO formulated to 18% CP and (3) 1.2% SID Lys; (4) 1.35% SID Lys by the addition of feed grade amino acids (AA), and (5) diet 4 with non-essential amino acids (NEAA; Gly and Glu). Pigs fed 21% CP with ZnO had increased (P = 0.001) ADG compared to those fed 18% CP (1.35% SID Lys) with high levels of feed grade amino acids or those fed the reduced SID Lys (1.2%) diet. Overall, G:F was improved (P < 0.001) for pigs fed 21% CP diets and those fed the 18% CP diet with NEAA compared to pigs fed 1.2% SID Lys and pigs fed high levels of feed grade amino acids. Fecal DM was increased for pigs fed the reduced SID Lys diet. In summary, pharmacological levels of Zn improve pig growth performance, but reducing CP (and subsequently SID Lys) decreased nursery pig growth performance. 相似文献
38.
Diane Frank Joel Dehasse 《Veterinary Clinics of North America: Small Animal Practice》2003,33(2):269-286
Human-directed aggression in cats should be evaluated as a multifactorial problem. It results from the combined actions of heredity, environment, learning, human social requirements (or needs), client interactions, lack of understanding of normal feline behavior, unrealistic client expectations, and lack of meeting the cat's basic ethologic needs. Managing human-directed aggression in cats encompasses the use of environmental modification, therapies, and, when and if needed, regulatory drugs so as to increase learning capabilities and adaptation and decrease danger to the human victims. 相似文献
39.
A review of aspects of diversity recently introduced in the statistical literature by Patil and Tallie is given: species diversity as an average property of a community is defined; the property averaged is identified as species rarity; and relations between common indices of diversity and associated measures of rarity are illuminated. This paper also computes common indices of diversity from substantial empirical data collected before clearcutting and site-preparation under two management regimes and for 3 years following planting of slash pine (Pinus elliottii) to illustrate that the initial response to these forest operations is increased species diversity, i.e., increased species rarity. This response may be expected whenever clearcutting and site preparation reduces but does not eliminate previously abundant, late-successional species and permits the reappearance of early-successional species, including forbs and grasses. 相似文献
40.
G L Gulliani A D Chanana E P Cronkite D D Joel J Laissue K R Rai 《American journal of veterinary research》1975,36(10):1469-1471
Apparent half-survival time of 51-Cr-tagged caprine erythrocytes after autotransfusion was 8 days. The 51Cr-tagged homologous caprine erythrocytes disappeared from circulation very rapidly, thereby indicating that it is not possible to produce sustained polycythemia in goats by hypertransfusing them with homologous erythrocytes without appropriate matching. 相似文献